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  1. Article ; Online: COVID-19: Multiorgan Dissemination of SARS-CoV-2 Is Driven by Pulmonary Factors.

    Odilov, Akmaljon / Volkov, Alexey / Abdullaev, Adhamjon / Gasanova, Tatiana / Lipina, Tatiana / Babichenko, Igor

    Viruses

    2021  Volume 14, Issue 1

    Abstract: Multi-organ failure is one of the common causes of fatal outcome in COVID-19 patients. However, the pathogenetic association of the SARS-CoV-2 viral load (VL) level with fatal dysfunctions of the lungs, liver, kidneys, heart, spleen and brain, as well as ...

    Abstract Multi-organ failure is one of the common causes of fatal outcome in COVID-19 patients. However, the pathogenetic association of the SARS-CoV-2 viral load (VL) level with fatal dysfunctions of the lungs, liver, kidneys, heart, spleen and brain, as well as with the risk of death in COVID-19 patients remains poorly understood. SARS-CoV-2 VL in the lungs, heart, liver, kidneys, brain, spleen and lymph nodes have been measured by RT qPCR using the following formula: N
    MeSH term(s) Aged ; Aged, 80 and over ; Autopsy ; COVID-19/pathology ; COVID-19/virology ; Female ; Humans ; Lung/pathology ; Lung/virology ; Male ; Middle Aged ; Multiple Organ Failure/pathology ; Multiple Organ Failure/virology ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/metabolism ; SARS-CoV-2/pathogenicity ; Viral Load ; Viral Proteins/metabolism
    Chemical Substances RNA, Viral ; Viral Proteins
    Language English
    Publishing date 2021-12-26
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14010039
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: COVID-19: Multiorgan Dissemination of SARS-CoV-2 Is Driven by Pulmonary Factors

    Odilov, Akmaljon / Volkov, Alexey / Abdullaev, Adhamjon / Gasanova, Tatiana / Lipina, Tatiana / Babichenko, Igor

    Viruses. 2021 Dec. 26, v. 14, no. 1

    2021  

    Abstract: Multi-organ failure is one of the common causes of fatal outcome in COVID-19 patients. However, the pathogenetic association of the SARS-CoV-2 viral load (VL) level with fatal dysfunctions of the lungs, liver, kidneys, heart, spleen and brain, as well as ...

    Abstract Multi-organ failure is one of the common causes of fatal outcome in COVID-19 patients. However, the pathogenetic association of the SARS-CoV-2 viral load (VL) level with fatal dysfunctions of the lungs, liver, kidneys, heart, spleen and brain, as well as with the risk of death in COVID-19 patients remains poorly understood. SARS-CoV-2 VL in the lungs, heart, liver, kidneys, brain, spleen and lymph nodes have been measured by RT qPCR using the following formula: NSᴬᴿS⁻Cᵒⱽ⁻²/Nᴬᴮᴸ¹ × 100. Dissemination of SARS-CoV-2 in 30.5% of cases was mono-organ, and in 63.9% of cases, it was multi-organ. The average SARS-CoV-2 VL in the exudative phase of diffuse alveolar damage (DAD) was 60 times higher than in the proliferative phase. The SARS-CoV-2 VL in the lungs ranged from 0 to 250,281 copies. The “pulmonary factors” of SARS-CoV-2 multi-organ dissemination are the high level of SARS-CoV-2 VL (≥4909) and the exudative phase of DAD. The frequency of SARS-CoV-2 dissemination to lymph nodes was 86.9%, heart–56.5%, spleen–52.2%, liver–47.8%, kidney–26%, and brain–13%. We found no link between the SARS-CoV-2 VL level in the liver, kidneys, and heart and the serum level of CPK, LDH, ALP, ALT, AST and Cr of COVID-19 patients. Isolated detection of SARS-CoV-2 RNA in the myocardium of COVID-19 patients who died from heart failure is possible. The pathogenesis of COVID-19-associated multi-organ failure requires further research in a larger cohort of patients.
    Keywords COVID-19 infection ; RNA ; Severe acute respiratory syndrome coronavirus 2 ; blood serum ; brain ; death ; heart failure ; liver ; lymph ; myocardium ; pathogenesis ; risk ; spleen ; viral load
    Language English
    Dates of publication 2021-1226
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14010039
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Viral Load and Patterns of SARS-CoV-2 Dissemination to the Lungs, Mediastinal Lymph Nodes, and Spleen of Patients with COVID-19 Associated Lymphopenia.

    Abdullaev, Adhamjon / Odilov, Akmaljon / Ershler, Maxim / Volkov, Alexey / Lipina, Tatiana / Gasanova, Tatiana / Lebedin, Yuri / Babichenko, Igor / Sudarikov, Andrey

    Viruses

    2021  Volume 13, Issue 7

    Abstract: Lymphopenia is a frequent hematological manifestation, associated with a severe course of COVID-19, with an insufficiently understood pathogenesis. We present molecular genetic immunohistochemical, and electron microscopic data on SARS-CoV-2 ... ...

    Abstract Lymphopenia is a frequent hematological manifestation, associated with a severe course of COVID-19, with an insufficiently understood pathogenesis. We present molecular genetic immunohistochemical, and electron microscopic data on SARS-CoV-2 dissemination and viral load (VL) in lungs, mediastinum lymph nodes, and the spleen of 36 patients who died from COVID-19. Lymphopenia <1 × 10
    MeSH term(s) Aged ; Aged, 80 and over ; COVID-19/virology ; COVID-19 Testing ; Female ; Humans ; Immunohistochemistry ; Lung/pathology ; Lung/virology ; Lymph Nodes/virology ; Lymphopenia/immunology ; Lymphopenia/virology ; Male ; Mediastinum/virology ; Middle Aged ; Multiplex Polymerase Chain Reaction ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Spike Glycoprotein, Coronavirus/genetics ; Spleen/virology ; Viral Load
    Chemical Substances Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2021-07-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13071410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Viral Load and Patterns of SARS-CoV-2 Dissemination to the Lungs, Mediastinal Lymph Nodes, and Spleen of Patients with COVID-19 Associated Lymphopenia

    Abdullaev, Adhamjon / Odilov, Akmaljon / Ershler, Maxim / Volkov, Alexey / Lipina, Tatiana / Gasanova, Tatiana / Lebedin, Yuri / Babichenko, Igor / Sudarikov, Andrey

    Viruses. 2021 July 20, v. 13, no. 7

    2021  

    Abstract: Lymphopenia is a frequent hematological manifestation, associated with a severe course of COVID-19, with an insufficiently understood pathogenesis. We present molecular genetic immunohistochemical, and electron microscopic data on SARS-CoV-2 ... ...

    Abstract Lymphopenia is a frequent hematological manifestation, associated with a severe course of COVID-19, with an insufficiently understood pathogenesis. We present molecular genetic immunohistochemical, and electron microscopic data on SARS-CoV-2 dissemination and viral load (VL) in lungs, mediastinum lymph nodes, and the spleen of 36 patients who died from COVID-19. Lymphopenia <1 × 10⁹/L was observed in 23 of 36 (63.8%) patients. In 12 of 36 cases (33%) SARS-CoV-2 was found in lung tissues only with a median VL of 239 copies (range 18–1952) SARS-CoV-2 cDNA per 100 copies of ABL1. Histomorphological changes corresponding to bronchopneumonia and the proliferative phase of DAD were observed in these cases. SARS-CoV-2 dissemination into the lungs, lymph nodes, and spleen was detected in 23 of 36 patients (58.4%) and was associated with the exudative phase of DAD in most of these cases. The median VL in the lungs was 12,116 copies (range 810–250281), lymph nodes—832 copies (range 96–11586), and spleen—71.5 copies (range 0–2899). SARS-CoV-2 in all cases belonged to the 19A strain. A immunohistochemical study revealed SARS-CoV-2 proteins in pneumocytes, alveolar macrophages, and bronchiolar epithelial cells in lung tissue, sinus histiocytes of lymph nodes, as well as cells of the Billroth pulp cords and spleen capsule. SARS-CoV-2 particles were detected by transmission electron microscopy in the cytoplasm of the endothelial cell, macrophages, and lymphocytes. The infection of lymphocytes with SARS-CoV-2 that we discovered for the first time may indicate a possible link between lymphopenia and SARS-CoV-2-mediated cytotoxic effect.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; bronchopneumonia ; cytoplasm ; cytotoxicity ; endothelial cells ; epithelium ; histiocytes ; immunohistochemistry ; lymph ; mediastinum ; pathogenesis ; pneumocytes ; pulp ; spleen ; transmission electron microscopy ; viral load
    Language English
    Dates of publication 2021-0720
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13071410
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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