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  1. Book ; Online: Southern Ocean Pleistocene calcareous nannofossil events: calibration with isotope and geomagnetic stratigraphies, supplementary data to: Flores, Jos? Abel; Gersonde, Rainer; Sierro, Francisco Javier; Niebler, Hans-Stefan (2000): Southern Ocean Pleistocene calcareous nannofossil events: calibration with isotope and geomagnetic stratigraphies. Marine Micropaleontology, 40(4), 377-402

    Flores, Jos? Abel / Gersonde, Rainer / Niebler, Hans-Stefan / Sierro, Francisco Javier

    2000  

    Abstract: Several cores recovered from the northern belt of the Southern Ocean were analysed to study the Pleistocene calcareous nannofossil records. Calcareous nannofossil events previously described in medium and low latitudes were identified and calibrated with ...

    Abstract Several cores recovered from the northern belt of the Southern Ocean were analysed to study the Pleistocene calcareous nannofossil records. Calcareous nannofossil events previously described in medium and low latitudes were identified and calibrated with the oxygen isotope and geomagnetic time scales. Although sedimentation rates, hiatuses and degree of calcareous nannofossil preservation sometimes prevent the identification and/or accurate calibration of some of these events, a useful stratigraphic framework was obtained. The possibility of using these calibrated events from high to low latitudes facilitates correlations and should facilitate isotope event identification in a region with low temperature, where calcareous plankton stratigraphies are in general restricted. In general, Pleistocene southern high latitude calcareous nannofossil events show synchronism with those observed in warm and temperate surficial waters. Small discrepancies in the assigned ages are sometimes related to low sampling resolution due to low sedimentation rates. The first occurrence (FO) of Emiliania huxleyi and the last occurrence (LO) of Pseudoemiliania lacunosa are observed in Marine Isotope Stages (MIS) 8 and 12, respectively. A reversal in abundance between Gephyrocapsa muellerae and E. huxleyi is observed close to the MIS 4/5 boundary. MIS 6 is characterised by an increase in G. muellerae and MIS 7 features a dramatic decrease in the proportion of Gephyrocapsa caribbeanica. This latter species began to increase its proportions from the MIS 13/14 boundary to MIS13, showing diachronism between the different sites. The LO of Reticulofenestra asanoi is observed at MIS 22, confirming this event as a global synchronous reference datum. By contrast, the FO of R. asanoi occurs at MIS 35 and is diachronous with the existing data from other oceanic regions. A re-entry of medium sized Gephyrocapsa (3-5 mm maximum diameters) can be identified in some cores close to MIS 25; although the low abundance of this taxon prevents an accurate calibration, it may be concluded that this event is diachronous as compared with the existing low-latitude data. The LO of large morphotypes of Gephyrocapsa is well correlated with MIS 37, showing synchronism with other oceanic regions, whereas the FO of this species is not well calibrated due to the absence of age-control points.
    Language English
    Dates of publication 2000-9999
    Size Online-Ressource
    Publisher PANGAEA - Data Publisher for Earth & Environmental Science
    Publishing place Bremen/Bremerhaven
    Document type Book ; Online
    Note This dataset is supplement to doi:10.1016/S0377-8398(00)00047-5
    DOI 10.1594/PANGAEA.679318
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  2. Article ; Online: Anaphylaxis induced bradycardia, renal failure, AV-nodal blockade, shock, and hyperkalemia: A-BRASH in the emergency department.

    Flores, Stefan

    The American journal of emergency medicine

    2020  Volume 38, Issue 9, Page(s) 1987.e1–1987.e3

    Abstract: The emergency department (ED) is responsible for managing a variety of acute illnesses including undifferentiated shock. A newer less recognized syndrome termed BRASH - bradycardia, renal failure, AV-node blockers, shock and hyperkalemia - is a cycle of ... ...

    Abstract The emergency department (ED) is responsible for managing a variety of acute illnesses including undifferentiated shock. A newer less recognized syndrome termed BRASH - bradycardia, renal failure, AV-node blockers, shock and hyperkalemia - is a cycle of synergy between hyperkalemia and AV-blockade that can result in shock. This entity is more common amongst the elderly, considering polypharmacy and co-morbid diseases. Some cases have an inciting trigger of hypovolemia. Anaphylaxis is a potentially lethal form of shock that most emergency physicians (EP) treat in the ED. The two entities have never occurred simultaneously. Herein, we report a case of anaphylaxis induced BRASH syndrome occurring in an elderly diabetic man. The EP should be aware of this unique presentation of BRASH. It was concluded that the patient's anaphylaxis, caused hypovolemia, coupled with polypharmacy, and physiological stress, precipitated this severe form of shock and multi-organ system failure.
    MeSH term(s) Aged ; Anaphylaxis/complications ; Atrioventricular Block/etiology ; Bradycardia/etiology ; Emergency Service, Hospital ; Epinephrine/therapeutic use ; Humans ; Hyperkalemia/etiology ; Male ; Renal Insufficiency/etiology ; Shock/etiology
    Chemical Substances Epinephrine (YKH834O4BH)
    Language English
    Publishing date 2020-05-16
    Publishing country United States
    Document type Case Reports
    ZDB-ID 605890-5
    ISSN 1532-8171 ; 0735-6757
    ISSN (online) 1532-8171
    ISSN 0735-6757
    DOI 10.1016/j.ajem.2020.05.033
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Suffocating in the eye of the storm: attempting to breathe at the epicentre of New York's COVID-19 pandemic.

    Jiang, Lynn / Flores, Stefan

    Emergency medicine journal : EMJ

    2020  Volume 37, Issue 6, Page(s) 330–331

    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections/epidemiology ; Coronavirus Infections/psychology ; Emergency Medicine/organization & administration ; Humans ; New York City/epidemiology ; Pandemics ; Physicians/psychology ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/psychology ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-05-03
    Publishing country England
    Document type Editorial
    ZDB-ID 2040124-3
    ISSN 1472-0213 ; 1472-0205
    ISSN (online) 1472-0213
    ISSN 1472-0205
    DOI 10.1136/emermed-2020-209825
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Phosphatidylserine-dependent structure of synaptogyrin remodels the synaptic vesicle membrane.

    Yu, Taekyung / Flores-Solis, David / Eastep, Gunnar N / Becker, Stefan / Zweckstetter, Markus

    Nature structural & molecular biology

    2023  Volume 30, Issue 7, Page(s) 926–934

    Abstract: Synaptic vesicles are small membrane-enclosed organelles that store neurotransmitters at presynaptic terminals. The uniform morphology of synaptic vesicles is important for brain function, because it enables the storage of well-defined amounts of ... ...

    Abstract Synaptic vesicles are small membrane-enclosed organelles that store neurotransmitters at presynaptic terminals. The uniform morphology of synaptic vesicles is important for brain function, because it enables the storage of well-defined amounts of neurotransmitters and thus reliable synaptic transmission. Here, we show that the synaptic vesicle membrane protein synaptogyrin cooperates with the lipid phosphatidylserine to remodel the synaptic vesicle membrane. Using NMR spectroscopy, we determine the high-resolution structure of synaptogyrin and identify specific binding sites for phosphatidylserine. We further show that phosphatidylserine binding changes the transmembrane structure of synaptogyrin and is critical for membrane bending and the formation of small vesicles. Cooperative binding of phosphatidylserine to both a cytoplasmic and intravesicular lysine-arginine cluster in synaptogyrin is required for the formation of small vesicles. Together with other synaptic vesicle proteins, synaptogyrin thus can sculpt the membrane of synaptic vesicles.
    MeSH term(s) Synaptogyrins/metabolism ; Synaptic Vesicles/metabolism ; Phosphatidylserines ; Nerve Tissue Proteins/chemistry ; Membrane Proteins/metabolism ; Synaptic Transmission
    Chemical Substances Synaptogyrins ; Phosphatidylserines ; Nerve Tissue Proteins ; Membrane Proteins
    Language English
    Publishing date 2023-05-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2126708-X
    ISSN 1545-9985 ; 1545-9993
    ISSN (online) 1545-9985
    ISSN 1545-9993
    DOI 10.1038/s41594-023-01004-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Neutrophil-like cells derived from the HL-60 cell-line as a genetically-tractable model for neutrophil degranulation.

    Bhakta, Suhani B / Lundgren, Stefan M / Sesti, Bethany N / Flores, Barbara A / Akdogan, Emel / Collins, Sean R / Mercer, Frances

    PloS one

    2024  Volume 19, Issue 2, Page(s) e0297758

    Abstract: Research on neutrophil biology has been limited by the short life span and limited genetic manipulability of these cells, driving the need for representative and efficient model cell lines. The promyelocytic cell line HL-60 and its subline PLB-985 can be ...

    Abstract Research on neutrophil biology has been limited by the short life span and limited genetic manipulability of these cells, driving the need for representative and efficient model cell lines. The promyelocytic cell line HL-60 and its subline PLB-985 can be differentiated into neutrophil-like cells (NLCs) and have been used to study neutrophil functions including chemotaxis, phagocytosis, endocytosis, and degranulation. Compared to neutrophils derived from hematopoietic stem cells, NLCs serve as a cost-effective neutrophil model. NLCs derived from both HL-60 and PLB-985 cells have been shown to perform degranulation, an important neutrophil function. However, no study has directly compared the two lines as models for degranulation including their release of different types of mobilizable organelles. Furthermore, Nutridoma, a commercially available supplement, has recently been shown to improve the chemotaxis, phagocytosis, and oxidative burst abilities of NLCs derived from promyelocytic cells, however it is unknown whether this reagent also improves the degranulation ability of NLCs. Here, we show that NLCs derived from both HL-60 and PLB-985 cells are capable of degranulating, with each showing markers for the release of multiple types of secretory organelles, including primary granules. We also show that differentiating HL-60 cells using Nutridoma does not enhance their degranulation activity over NLCs differentiated using Dimethyl Sulfoxide (DMSO) plus Granulocyte-colony stimulating factor (G-CSF). Finally, we show that promyelocytic cells can be genetically engineered and differentiated using these methods, to yield NLCs with a defect in degranulation. Our results indicate that both cell lines serve as effective models for investigating the mechanisms of neutrophil degranulation, which can advance our understanding of the roles of neutrophils in inflammation and immunity.
    MeSH term(s) Humans ; Neutrophils/metabolism ; HL-60 Cells ; Phagocytosis ; Cell Differentiation/physiology ; Granulocyte Precursor Cells ; Cell Degranulation
    Language English
    Publishing date 2024-02-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0297758
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Online: Suffocating in the eye of the storm

    Jiang, Lynn / Flores, Stefan

    attempting to breathe at the epicentre of New Yorks COVID-19 pandemic

    2020  

    Keywords The view from here ; covid19
    Language English
    Publishing date 2020-06-01 00:00:00.0
    Publisher BMJ Publishing Group Ltd
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: The ternary phase diagram of nitrogen doped lutetium hydrides can not explain its claimed high Tc superconductivity

    Moritz Gubler / Marco Krummenacher / Jonas A Finkler / José A Flores-Livas / Stefan Goedecker

    New Journal of Physics, Vol 25, Iss 12, p

    2023  Volume 123008

    Abstract: This paper presents the results of an extensive structural search of ternary solids containing lutetium, nitrogen and hydrogen. Based on thousands of thermodynamically stable structures the convex hull of the formation enthalpies is constructed. To ... ...

    Abstract This paper presents the results of an extensive structural search of ternary solids containing lutetium, nitrogen and hydrogen. Based on thousands of thermodynamically stable structures the convex hull of the formation enthalpies is constructed. To obtain the correct energetic ordering, the highly accurate RSCAN DFT functional is used in high quality all-electron calculations, eliminating possible pseudopotential errors. In this way, a novel lutetium hydride structure (HLu _2 ) is found that is on the convex hull. An electron phonon analysis however shows that it is not a candidate structure for near ambient superconductivity. Besides this structure, which appears to have been missed in previous searches, possibly due to different DFT methodologies, our results agree closely with the results of previously published structure search efforts. This shows, that the field of crystal structure prediction has matured to a state where independent methodologies produce consistent and reproducible results, underlining the trustworthiness of modern crystal structure predictions. Hence it is quite unlikely that a structure, that would give rise within standard BCS theory to the superconducting properties, claimed to have been observed by Dasenbrock-Gammon et al (2023 Nature 615 244), exists. This solidifies the evidence that structures with high T _c conventional superconductivity, that could give rise to the experimental claims, do not exist in this material.
    Keywords room temperature superconductivity ; structure prediction ; electronic structure calculations ; Science ; Q ; Physics ; QC1-999
    Subject code 540
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher IOP Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Suffocating in the eye of the storm

    Jiang, Lynn / Flores, Stefan

    Emergency Medicine Journal

    attempting to breathe at the epicentre of New York’s COVID-19 pandemic

    2020  Volume 37, Issue 6, Page(s) 330–331

    Keywords Critical Care and Intensive Care Medicine ; Emergency Medicine ; General Medicine ; covid19
    Language English
    Publisher BMJ
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 2040124-3
    ISSN 1472-0205
    ISSN 1472-0205
    DOI 10.1136/emermed-2020-209825
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Genome sequences of BCG Pasteur ATCC 35734 and its derivative, the vaccine candidate BCGΔBCG1419c.

    D'Auria, Giuseppe / Hodzhev, Yordan / Aceves-Sánchez, Michel de Jesús / Moya, Andrés / Panaiotov, Stefan / Flores-Valdez, Mario Alberto

    BMC genomics

    2023  Volume 24, Issue 1, Page(s) 69

    Abstract: Background: Bacillus Calmette-Guérin (BCG) remains the only vaccine to prevent tuberculosis (TB) during childhood, with relatively low to no efficacy against pulmonary TB in adolescents and adults. BCG consists of close to 15 different substrains, where ...

    Abstract Background: Bacillus Calmette-Guérin (BCG) remains the only vaccine to prevent tuberculosis (TB) during childhood, with relatively low to no efficacy against pulmonary TB in adolescents and adults. BCG consists of close to 15 different substrains, where genetic variations among them might contribute to the variable protective efficacy afforded against pulmonary TB. We have shown that the vaccine candidate, BCGΔBCG1419c, which is based on BCG Pasteur, improved protection against chronic TB in murine models, as well as against pulmonary and extrapulmonary TB in guinea pigs. Here, to confirm deletion of the BCG1419c gene and to detect possible genetic variations occurring as a consequence of the spontaneous mutations that may arise during in vitro culture of mycobacteria, the genomes of BCG Pasteur ATCC 35734 and its isogenic derivative, BCGΔBCG1419c, were sequenced and subjected to a comparative analysis between them and against BCG Pasteur 1173P2.
    Results: The complete catalog of variants in genes relative to the reference genome BCG Pasteur 1173P2 (GenBank NC008769) showed that the parental strain BCG Pasteur ATCC 35734, from which the mutant BCGΔBCG1419c originated, showed five synonymous mutations, three missense mutations, and five codon insertions, whereas the BCGΔBCG1419c mutant reported the same changes. When BCG Pasteur ATCC 35734 and BCGΔBCG1419c were compared, we confirmed that the latter was devoid of the BCG1419c gene, with only one unanticipated SNP at position 2, 828, 791  which we consider has no role in vaccine properties reported thus far.
    Conclusion: We provide evidence that the mutagenesis performed to remove BCG1419c from BCG Pasteur ATCC 35734 solely deleted this gene, and that compared with the reference strain BCG Pasteur 1173P2, few changes were present confirming that they are BCG Pasteur strains, and that changes in immunogenicity or efficacy observed thus far in BCGΔBCG1419c are most likely derived solely from the elimination of the BCG1419c gene.
    MeSH term(s) Animals ; Mice ; Guinea Pigs ; BCG Vaccine/genetics ; Mycobacterium bovis/genetics ; Tuberculosis/microbiology ; Tuberculosis, Pulmonary ; Genome
    Chemical Substances BCG Vaccine
    Language English
    Publishing date 2023-02-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041499-7
    ISSN 1471-2164 ; 1471-2164
    ISSN (online) 1471-2164
    ISSN 1471-2164
    DOI 10.1186/s12864-023-09169-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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