Article ; Online: Autoantibodies to survival of motor neuron complex in patients with polymyositis: immunoprecipitation of D, E, F, and G proteins without other components of small nuclear ribonucleoproteins.
2011 Volume 63, Issue 7, Page(s) 1972–1978
Abstract: ... of (35) S-methionine-labeled cell extracts. Sera with which D, E, F, and G proteins of snRNP was ... immunofluorescence.: Results: Three sera that immunoprecipitated D, E, F, and G proteins without other components ... of the diagnosis or of certain clinical characteristics. An unusual pattern of immunoprecipitation, in which the D ...
| Abstract | Objective: Autoantibodies in the systemic rheumatic diseases are clinically useful biomarkers of the diagnosis or of certain clinical characteristics. An unusual pattern of immunoprecipitation, in which the D, E, F, and G proteins of small nuclear RNPs (snRNP) but without other components of the snRNP, was noticed at the autoantibody screening. The purpose of this study was to examine the target antigens and clinical manifestations associated with this specificity. Methods: Autoantibodies in sera from 1,966 American patients (including 434 with systemic lupus erythematosus, 121 with scleroderma, 86 with polymyositis/dermatomyositis [PM/DM]) and 248 Italian patients with autoimmune diseases were screened by immunoprecipitation of (35) S-methionine-labeled cell extracts. Sera with which D, E, F, and G proteins of snRNP was immunoprecipitated, but without the other snRNP proteins, were further examined by analysis of RNA components by immunoprecipitation (silver staining), Western blotting using survival of motor neuron (SMN) complex, and immunofluorescence. Results: Three sera that immunoprecipitated D, E, F, and G proteins without other components (U1-70K, A, B'/B, C) of the snRNP were found. Four additional proteins (130 kd, 120 kd, 38 kd, and 33 kd) were also commonly immunoprecipitated. The target antigen was identified as SMN complex (Gemin 3, Gemin 4, SMN, and Gemin 2, respectively), which plays a critical role in the assembly of snRNP. In immunofluorescence analyses, all 3 sera showed nuclear dots (Cajal bodies) and cytoplasmic staining. Only 1 serum was weakly positive on Western blotting of SMN, suggesting that these sera mainly recognize native molecule or quaternary structure. All 3 patients were white women with PM, an interesting finding, since deletion or mutation of SMN is known to cause spinal muscular atrophy. Conclusion: SMN complex was identified as a new Cajal body autoantigen recognized by sera from white patients with PM. The biologic and clinical significance of anti-SMN autoantibodies will need to be clarified. |
|---|---|
| MeSH term(s) | Adult ; Autoantibodies/immunology ; Autoimmune Diseases/immunology ; Blotting, Western ; Female ; Fluorescent Antibody Technique ; Humans ; Immunoprecipitation ; Lupus Erythematosus, Systemic/immunology ; Male ; Middle Aged ; Polymyositis/immunology ; Ribonucleoproteins, Small Nuclear/immunology ; SMN Complex Proteins/immunology ; Scleroderma, Systemic/immunology |
| Chemical Substances | Autoantibodies ; Ribonucleoproteins, Small Nuclear ; SMN Complex Proteins |
| Language | English |
| Publishing date | 2011-03-21 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 127294-9 |
| ISSN | 1529-0131 ; 0004-3591 ; 2326-5191 |
| ISSN (online) | 1529-0131 |
| ISSN | 0004-3591 ; 2326-5191 |
| DOI | 10.1002/art.30349 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 24: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
|||
| Zs.MO 523: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.