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Article ; Online: Biotechnologies and Strategies for Grapevine Improvement

Butiuc-Keul, Anca / Coste, Ana

Horticulturae. 2023 Jan. 04, v. 9, no. 1

2023

Abstract: Grapevine (Vitis vinifera subsp. vinifera) is one of the most widespread and economically important perennial fruit crops in the world. Viticulture has changed over the years in response to changing environmental conditions and market demands, triggering ...

Abstract	Grapevine (Vitis vinifera subsp. vinifera) is one of the most widespread and economically important perennial fruit crops in the world. Viticulture has changed over the years in response to changing environmental conditions and market demands, triggering the development of new and improved varieties to ensure the crop’s sustainability. The aim of this review is to provide a perspective on the recent developments in biotechnology and molecular biology and to establish the potential of these technologies for the genetic improvement of grapevine. The following aspects are discussed: (i) the importance of molecular marker-based methods for proper cultivar identification and how NGS-based high-throughput technologies have greatly benefited the development of genotyping techniques, trait mapping, and genomic selection; (ii) the recent advances in grapevine regeneration, genetic transformation, and genome editing, such as new breeding technology approaches for enhanced grapevine yield, quality improvement, and the selection of valuable varieties and cultivars. The specific problems and challenges linked to grapevine biotechnology, along with the importance of integrating classical and new technologies, are highlighted.
Keywords	Vitis ; biotechnology ; cultivar identification ; cultivars ; fruits ; genetic improvement ; genetic transformation ; genome ; genotyping ; marker-assisted selection ; markets ; molecular biology ; viticulture
Language	English
Dates of publication	2023-0104
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2813983-5
ISSN	2311-7524
ISSN	2311-7524
DOI	10.3390/horticulturae9010062
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Double-Network Chitosan-Based Hydrogels with Improved Mechanical, Conductive, Antimicrobial, and Antibiofouling Properties.

Carpa, Rahela / Farkas, Anca / Dobrota, Cristina / Butiuc-Keul, Anca

Gels (Basel, Switzerland)

2023 Volume 9, Issue 4

Abstract: In recent years, the antimicrobial activity of chitosan-based hydrogels has been at the forefront of research in wound healing and the prevention of medical device contamination. Anti-infective therapy is a serious challenge given the increasing ... ...

Abstract	In recent years, the antimicrobial activity of chitosan-based hydrogels has been at the forefront of research in wound healing and the prevention of medical device contamination. Anti-infective therapy is a serious challenge given the increasing prevalence of bacterial resistance to antibiotics as well as their ability to form biofilms. Unfortunately, hydrogel resistance and biocompatibility do not always meet the demands of biomedical applications. As a result, the development of double-network hydrogels could be a solution to these issues. This review discusses the most recent techniques for creating double-network chitosan-based hydrogels with improved structural and functional properties. The applications of these hydrogels are also discussed in terms of tissue recovery after injuries, wound infection prevention, and biofouling of medical devices and surfaces for pharmaceutical and medical applications.
Language	English
Publishing date	2023-03-29
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2813982-3
ISSN	2310-2861 ; 2310-2861
ISSN (online)	2310-2861
ISSN	2310-2861
DOI	10.3390/gels9040278
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Phytochemical Characterization and Antimicrobial Activity of Several

Barbu, Ioana Andreea / Ciorîță, Alexandra / Carpa, Rahela / Moț, Augustin Catalin / Butiuc-Keul, Anca / Pârvu, Marcel

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 10

Abstract: Microbial infections affect both the human population and animals. The appearance of more and more microbial strains resistant to classical treatments led to the need to develop new treatments. ...

Abstract	Microbial infections affect both the human population and animals. The appearance of more and more microbial strains resistant to classical treatments led to the need to develop new treatments.
MeSH term(s)	Animals ; Humans ; Allium/chemistry ; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Anti-Infective Agents/pharmacology ; Anti-Infective Agents/chemistry ; Garlic/chemistry ; Staphylococcus aureus ; Polyphenols/pharmacology ; Phytochemicals/pharmacology ; Flavonoids/pharmacology
Chemical Substances	allicin (3C39BY17Y6) ; Plant Extracts ; Anti-Infective Agents ; Polyphenols ; Phytochemicals ; Flavonoids
Language	English
Publishing date	2023-05-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28103980
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Inherent and Composite Hydrogels as Promising Materials to Limit Antimicrobial Resistance.

Carpa, Rahela / Remizovschi, Alexei / Culda, Carla Andreea / Butiuc-Keul, Anca Livia

Gels (Basel, Switzerland)

2022 Volume 8, Issue 2

Abstract: Antibiotic resistance has increased significantly in the recent years, and has become a global problem for human health and the environment. As a result, several technologies for the controlling of health-care associated infections have been developed ... ...

Abstract	Antibiotic resistance has increased significantly in the recent years, and has become a global problem for human health and the environment. As a result, several technologies for the controlling of health-care associated infections have been developed over the years. Thus, the most recent findings in hydrogel fabrication, particularly antimicrobial hydrogels, could offer valuable solutions for these biomedical challenges. In this review, we discuss the most promising strategies in the development of antimicrobial hydrogels and the application of hydrogels in the treatment of microbial infections. The latest advances in the development of inherently and composite antimicrobial hydrogels will be discussed, as well as hydrogels as carriers of antimicrobials, with a focus on antibiotics, metal nanoparticles, antimicrobial peptides, and biological extracts. The emergence of CRISR-Cas9 technology for removing the antimicrobial resistance has led the necessity of new and performant carriers for delivery of the CRISPR-Cas9 system. Different delivery systems, such as composite hydrogels and many types of nanoparticles, attracted a great deal of attention and will be also discussed in this review.
Language	English
Publishing date	2022-01-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2813982-3
ISSN	2310-2861 ; 2310-2861
ISSN (online)	2310-2861
ISSN	2310-2861
DOI	10.3390/gels8020070
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Molecular Typing Reveals Environmental Dispersion of Antibiotic-Resistant Enterococci under Anthropogenic Pressure.

Farkas, Anca / Coman, Cristian / Szekeres, Edina / Teban-Man, Adela / Carpa, Rahela / Butiuc-Keul, Anca

Antibiotics (Basel, Switzerland)

2022 Volume 11, Issue 9

Abstract: As a consequence of global demographic challenges, both the artificial and the natural environment are increasingly impacted by contaminants of emerging concern, such as bacterial pathogens and their antibiotic resistance genes (ARGs). The aim of this ... ...

Abstract	As a consequence of global demographic challenges, both the artificial and the natural environment are increasingly impacted by contaminants of emerging concern, such as bacterial pathogens and their antibiotic resistance genes (ARGs). The aim of this study was to determine the extent to which anthropogenic contamination contributes to the spread of antibiotic resistant enterococci in aquatic compartments and to explore genetic relationships among
Language	English
Publishing date	2022-09-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics11091213
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: CRISPR-Cas System: The Powerful Modulator of Accessory Genomes in Prokaryotes.

Butiuc-Keul, Anca / Farkas, Anca / Carpa, Rahela / Iordache, Dumitrana

Microbial physiology

2021 Volume 32, Issue 1-2, Page(s) 2–17

Abstract: Being frequently exposed to foreign nucleic acids, bacteria and archaea have developed an ingenious adaptive defense system, called CRISPR-Cas. The system is composed of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) array, ... ...

Abstract	Being frequently exposed to foreign nucleic acids, bacteria and archaea have developed an ingenious adaptive defense system, called CRISPR-Cas. The system is composed of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) array, together with CRISPR (cas)-associated genes. This system consists of a complex machinery that integrates fragments of foreign nucleic acids from viruses and mobile genetic elements (MGEs), into CRISPR arrays. The inserted segments (spacers) are transcribed and then used by cas proteins as guide RNAs for recognition and inactivation of the targets. Different types and families of CRISPR-Cas systems consist of distinct adaptation and effector modules with evolutionary trajectories, partially independent. The origin of the effector modules and the mechanism of spacer integration/deletion is far less clear. A review of the most recent data regarding the structure, ecology, and evolution of CRISPR-Cas systems and their role in the modulation of accessory genomes in prokaryotes is proposed in this article. The CRISPR-Cas system's impact on the physiology and ecology of prokaryotes, modulation of horizontal gene transfer events, is also discussed here. This system gained popularity after it was proposed as a tool for plant and animal embryo editing, in cancer therapy, as antimicrobial against pathogenic bacteria, and even for combating the novel coronavirus - SARS-CoV-2; thus, the newest and promising applications are reviewed as well.
MeSH term(s)	Archaea/genetics ; Bacteria/genetics ; COVID-19/genetics ; CRISPR-Cas Systems/genetics ; Humans ; SARS-CoV-2/genetics
Language	English
Publishing date	2021-06-30
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	3042601-7
ISSN	2673-1673 ; 2673-1665
ISSN (online)	2673-1673
ISSN	2673-1665
DOI	10.1159/000516643
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Correlation between CRISPR Loci Diversity in Three Enterobacterial Taxa.

Iordache, Dumitrana / Baci, Gabriela-Maria / Căpriță, Oana / Farkas, Anca / Lup, Andreea / Butiuc-Keul, Anca

International journal of molecular sciences

2022 Volume 23, Issue 21

Abstract: CRISPR-Cas is an adaptive immunity system of prokaryotes, composed of CRISPR arrays and the associated proteins. The successive addition of spacer sequences in the CRISPR array has made the system a valuable molecular marker, with multiple applications. ... ...

Abstract	CRISPR-Cas is an adaptive immunity system of prokaryotes, composed of CRISPR arrays and the associated proteins. The successive addition of spacer sequences in the CRISPR array has made the system a valuable molecular marker, with multiple applications. Due to the high degree of polymorphism of the CRISPR loci, their comparison in bacteria from various sources may provide insights into the evolution and spread of the CRISPR-Cas systems. The aim of this study was to establish a correlation between the enterobacterial CRISPR loci, the sequence of direct repeats (DR), and the number of spacer units, along with the geographical origin and collection source. For this purpose, 3474 genomes containing CRISPR loci from the CRISPRCasdb of Salmonella enterica, Escherichia coli, and Klebsiella pneumoniae were analyzed, and the information regarding the isolates was recorded from the NCBI database. The most prevalent was the I-E CRISPR-Cas system in all three studied taxa. E. coli also presents the I-F type, but in a much lesser percentage. The systems found in K. pneumoniae can be classified into I-E and I-E. The I-E and I-F systems have two CRISPR loci, while I-E has only one locus upstream of the Cas cluster. PCR primers have been developed in this study for each CRISPR locus. Distinct clustering was not evident, but statistically significant relationships occurred between the different CRISPR loci and the number of spacer units. For each of the queried taxa, the number of spacers was significantly different (p < 0.01) by origin (Africa, Asia, Australia and Oceania, Europe, North America, and South America) but was not linked to the isolation source type (human, animal, plant, food, or laboratory strains).
MeSH term(s)	Humans ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; CRISPR-Cas Systems/genetics ; Enterobacteriaceae/genetics ; Escherichia coli/genetics ; Klebsiella pneumoniae/genetics ; Animals
Language	English
Publishing date	2022-10-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms232112766
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Impact of Arieş River Contaminants on Algae and Plants.

Halmagyi, Adela / Butiuc-Keul, Anca / Keul, Martin / Dobrotă, Cristina / Fodorpataki, László / Pintea, Adela / Mocan, Aurel / Pop, Valeria / Coste, Ana

Toxics

2023 Volume 11, Issue 10

Abstract: The Arieş River (Western Romania) represents one of the most important affluents of the Mureş River, with great significance in the Mureş Tisza basin. The environmental quality of the Arieş basin is significantly affected by both historic mining ... ...

Abstract	The Arieş River (Western Romania) represents one of the most important affluents of the Mureş River, with great significance in the Mureş Tisza basin. The environmental quality of the Arieş basin is significantly affected by both historic mining activities and contemporary impacts. Thus, an evaluation of the effects of the main contaminants found in water (organochlorine pesticides-OCPs, monocyclic aromatic hydrocarbons-MAHs, polycyclic aromatic hydrocarbons-PAHs, and metals) on cyanobacteria and plants was performed. Among OCPs, hexachlorocyclohexane isomers, dichlorodiphenyltrichloroethane, and derivatives were detected in plants while admissible concentrations were detected in water. Among MAHs, high levels of benzene were detected both in water and in plants. The levels of PAHs exceeded the allowable values in all samples. Increased concentrations of metals in water were found only at Baia de Arieş, but in plants, all metal concentrations were high. The pH, nitrates, nitrites, and phosphates, as well as metals, pesticides, and aromatic hydrocarbons, influenced the physiological characteristics of algae, test plants, and aquatic plants exposed to various compounds dissolved in water. Considering that the Arieş River basin is the site of intense past mining activities, these data provide information about the impact on water quality as a consequence of pollution events.
Language	English
Publishing date	2023-09-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2733883-6
ISSN	2305-6304 ; 2305-6304
ISSN (online)	2305-6304
ISSN	2305-6304
DOI	10.3390/toxics11100817
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Antibiotic resistance profiling of pathogenic Enterobacteriaceae from Cluj-Napoca, Romania.

Farkas, Anca / Tarco, Emma / Butiuc-Keul, Anca

Germs

2019 Volume 9, Issue 1, Page(s) 17–27

Abstract: Introduction: Members of the family Enterobacteriaceae are commonly identified in the clinical laboratory, being responsible for a substantial range of infections. This study aimed to investigate phenotypic and genotypic resistance traits in pathogenic ... ...

Abstract	Introduction: Members of the family Enterobacteriaceae are commonly identified in the clinical laboratory, being responsible for a substantial range of infections. This study aimed to investigate phenotypic and genotypic resistance traits in pathogenic Enterobacteriaceae isolated from outpatients in Cluj-Napoca, Romania. Methods: Pathogenic Enterobacteriaceae were isolated from urinary tract infections, wound infections and persistent diarrhea in a private laboratory from Cluj-Napoca, Romania. Bacterial strains were biochemically identified and subjected to antimicrobial susceptibility testing by disk diffusion. The carriage of antibiotic resistance genes and of class 1 integron were assessed by PCR. Results: E. coli Conclusions: An important proportion of pathogenic Enterobacteriaceae were multidrug resistant, due to a wide diversity of mechanisms encoding genetic resistance.
Language	English
Publishing date	2019-03-01
Publishing country	Romania
Document type	Journal Article
ZDB-ID	2649305-6
ISSN	2248-2997
ISSN	2248-2997
DOI	10.18683/germs.2019.1153
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Correlation between CRISPR Loci Diversity in Three Enterobacterial Taxa

Dumitrana Iordache / Gabriela-Maria Baci / Oana Căpriță / Anca Farkas / Andreea Lup / Anca Butiuc-Keul

International Journal of Molecular Sciences, Vol 23, Iss 12766, p

2022 Volume 12766

Abstract	CRISPR-Cas is an adaptive immunity system of prokaryotes, composed of CRISPR arrays and the associated proteins. The successive addition of spacer sequences in the CRISPR array has made the system a valuable molecular marker, with multiple applications. Due to the high degree of polymorphism of the CRISPR loci, their comparison in bacteria from various sources may provide insights into the evolution and spread of the CRISPR-Cas systems. The aim of this study was to establish a correlation between the enterobacterial CRISPR loci, the sequence of direct repeats (DR), and the number of spacer units, along with the geographical origin and collection source. For this purpose, 3474 genomes containing CRISPR loci from the CRISPRCasdb of Salmonella enterica, Escherichia coli, and Klebsiella pneumoniae were analyzed, and the information regarding the isolates was recorded from the NCBI database. The most prevalent was the I-E CRISPR-Cas system in all three studied taxa. E. coli also presents the I-F type, but in a much lesser percentage. The systems found in K. pneumoniae can be classified into I-E and I-E. The I-E and I-F systems have two CRISPR loci, while I-E has only one locus upstream of the Cas cluster. PCR primers have been developed in this study for each CRISPR locus. Distinct clustering was not evident, but statistically significant relationships occurred between the different CRISPR loci and the number of spacer units. For each of the queried taxa, the number of spacers was significantly different ( p < 0.01) by origin (Africa, Asia, Australia and Oceania, Europe, North America, and South America) but was not linked to the isolation source type (human, animal, plant, food, or laboratory strains).
Keywords	CRISPR-Cas ; loci polymorphism ; PCR primers ; Salmonella enterica ; Escherichia coli ; Klebsiella pneumoniae ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	630
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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