Result 1 - 10 of total 22
Untersuchung von Protein-Protein-Interaktionen
2007
| Author's details | von Isabell Christine Erler |
|---|---|
| Keywords | Biowissenschaften, Biologie ; Life Science, Biology |
| Subject code | sg570 |
| Document type | Book ; Online ; Thesis |
| Database | Digital theses on the web |
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2023 Volume 11, Issue 7, Page(s) 946–961
Abstract: Despite the remarkable success of autologous chimeric antigen receptor (CAR) T cells, some patients relapse due to tumor antigen escape and low or uneven antigen expression, among other mechanisms. Therapeutic options after relapse are limited, ... ...
| Abstract | Despite the remarkable success of autologous chimeric antigen receptor (CAR) T cells, some patients relapse due to tumor antigen escape and low or uneven antigen expression, among other mechanisms. Therapeutic options after relapse are limited, emphasizing the need to optimize current approaches. In addition, there is a need to develop allogeneic "off-the-shelf" therapies from healthy donors that are readily available at the time of treatment decision and can overcome limitations of current autologous approaches. To address both challenges simultaneously, we generated a CD20xCD22 dual allogeneic CAR T cell. Herein, we demonstrate that allogeneic CD20x22 CAR T cells display robust, sustained and dose-dependent activity in vitro and in vivo, while efficiently targeting primary B-cell non-Hodgkin lymphoma (B-NHL) samples with heterogeneous levels of CD22 and CD20. Altogether, we provide preclinical proof-of-concept data for an allogeneic dual CAR T cell to overcome current mechanisms of resistance to CAR T-cell therapies in B-NHL, while providing a potential alternative to CD19 targeting. |
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| MeSH term(s) | Humans ; Receptors, Antigen, T-Cell ; Neoplasm Recurrence, Local ; T-Lymphocytes ; B-Lymphocytes ; Lymphoma, B-Cell ; Immunotherapy, Adoptive ; Antigens, CD19 ; Hematopoietic Stem Cell Transplantation |
| Chemical Substances | Receptors, Antigen, T-Cell ; Antigens, CD19 |
| Language | English |
| Publishing date | 2023-05-31 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2732489-8 |
| ISSN | 2326-6074 ; 2326-6066 |
| ISSN (online) | 2326-6074 |
| ISSN | 2326-6066 |
| DOI | 10.1158/2326-6066.CIR-22-0910 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 7022: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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Journal of law and the biosciences
2023 Volume 10, Issue 2, Page(s) lsad034
Abstract: Epigenetic research has brought several important technological achievements, including identifying epigenetic clocks and signatures, and developing epigenetic editing. The potential military applications of such technologies we discuss are stratifying ... ...
| Abstract | Epigenetic research has brought several important technological achievements, including identifying epigenetic clocks and signatures, and developing epigenetic editing. The potential military applications of such technologies we discuss are stratifying soldiers' health, exposure to trauma using epigenetic testing, information about biological clocks, confirming child soldiers' minor status using epigenetic clocks, and inducing epigenetic modifications in soldiers. These uses could become a reality. This article presents a comprehensive literature review, and analysis by interdisciplinary experts of the scientific, legal, ethical, and societal issues surrounding epigenetics and the military. Notwithstanding the potential benefit from these applications, our findings indicate that the current lack of scientific validation for epigenetic technologies suggests a careful scientific review and the establishment of a robust governance framework before consideration for use in the military. In this article, we highlight general concerns about the application of epigenetic technologies in the military context, especially discrimination and data privacy issues if soldiers are used as research subjects. We also highlight the potential of epigenetic clocks to support child soldiers' rights and ethical questions about using epigenetic engineering for soldiers' enhancement and conclude with considerations for an ethical framework for epigenetic applications in the military, defense, and security contexts. |
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| Language | English |
| Publishing date | 2023-12-13 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2756090-9 |
| ISSN | 2053-9711 |
| ISSN | 2053-9711 |
| DOI | 10.1093/jlb/lsad034 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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The Journal of biological chemistry
2004 Volume 279, Issue 33, Page(s) 34456–34463
Abstract: Transient receptor potential (TRP) proteins form cation-conducting ion channels with currently 28 known genes encoding TRP channel monomers in mammals. These monomers are thought to coassemble to form homo- or heterotetrameric channels, but the signals ... ...
| Abstract | Transient receptor potential (TRP) proteins form cation-conducting ion channels with currently 28 known genes encoding TRP channel monomers in mammals. These monomers are thought to coassemble to form homo- or heterotetrameric channels, but the signals governing their assembly are unknown. Within the TRPV subgroup, TRPV5 and TRPV6 show exclusive calcium selectivity and play an important role in calcium uptake. To identify signals that mediate assembly of functional TRPV6, we screened domains for self-association using co-immunoprecipitation, sucrose gradient centrifugation, bacterial two-hybrid assays, and patch clamp analysis. Of the two identified interaction domains within the N-terminal region, we showed that the first domain encompassing the third ankyrin repeat is the stringent requirement for physical assembly of TRPV6 subunits and when transferred to an unrelated protein enables its interaction with TRPV6. Deletion of this repeat or mutation of critical residues within this repeat rendered nonfunctional channels that do not co-immunoprecipitate or form tetramers. Suppression of dominant-negative inhibitors of TRPV6-specific currents was achieved by deletion of ankyrin (ANK) 3. We propose that the third ANK repeat initiates a molecular zippering process that proceeds past the fifth ANK repeat and creates an intracellular anchor that is necessary for functional subunit assembly. |
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| MeSH term(s) | Amino Acid Sequence ; Ankyrins/chemistry ; Biotinylation ; Calcium/metabolism ; Calcium Channels/chemistry ; Calcium Channels/physiology ; Cell Line ; Centrifugation, Density Gradient ; Gene Deletion ; Genes, Dominant ; Green Fluorescent Proteins ; Humans ; Ions ; Luminescent Proteins/metabolism ; Microscopy, Fluorescence ; Models, Biological ; Models, Molecular ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Mutation ; Oligonucleotides, Antisense/chemistry ; Patch-Clamp Techniques ; Precipitin Tests ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry ; Sequence Homology, Amino Acid ; Sucrose/pharmacology ; TRPV Cation Channels ; Transfection ; Two-Hybrid System Techniques |
| Chemical Substances | Ankyrins ; Calcium Channels ; Ions ; Luminescent Proteins ; Oligonucleotides, Antisense ; Recombinant Proteins ; TRPV Cation Channels ; TRPV5 protein, human ; TRPV6 channel ; Green Fluorescent Proteins (147336-22-9) ; Sucrose (57-50-1) ; Calcium (SY7Q814VUP) |
| Language | English |
| Publishing date | 2004-06-10 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2997-x |
| ISSN | 1083-351X ; 0021-9258 |
| ISSN (online) | 1083-351X |
| ISSN | 0021-9258 |
| DOI | 10.1074/jbc.M404778200 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Uc I Zs.108: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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Journal of biological chemistry. 2006 Dec. 15, v. 281, no. 50
2006
Abstract: TRPM (transient receptor potential melastatin-like) channels are distinct from many other members of the transient receptor potential family in regard to their overall size (>1000 amino acids), the lack of N-terminal ankyrin-like repeats, and ... ...
| Abstract | TRPM (transient receptor potential melastatin-like) channels are distinct from many other members of the transient receptor potential family in regard to their overall size (>1000 amino acids), the lack of N-terminal ankyrin-like repeats, and hydrophobicity predictions that may allow for more than six transmembrane regions. Common to each TRPM member is a prominent C-terminal coiled coil region. Here we have shown that TRPM8 channels assemble as multimers using the putative coiled coil region within the intracellular C terminus and that this assembly can be disturbed by a single point mutation within the coiled coil region. This mutant neither gives rise to functional channels nor do its subunits interact or form protein complexes that correspond to a multimer. However, they are still transported to the plasma membrane. Furthermore, wild-type currents can be suppressed by expressing the membrane-attached C-terminal region of TRPM8. To separate assembly from trafficking, we investigated the maturation of TRPM8 protein by identifying and mutating the relevant N-linked glycosylation site and showing that glycosylation is neither essential for multimerization nor for transport to the plasma membrane per se but appears to facilitate efficient multimerization and transport. |
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| Language | English |
| Dates of publication | 2006-1215 |
| Size | p. 38396-38404. |
| Publishing place | American Society for Biochemistry and Molecular Biology |
| Document type | Article |
| ZDB-ID | 2997-x |
| ISSN | 1083-351X ; 0021-9258 |
| ISSN (online) | 1083-351X |
| ISSN | 0021-9258 |
| Database | NAL-Catalogue (AGRICOLA) |
| Z 4' 65/118: Show issues | ||||
| Z 6.2: Show issues |
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eine Geschichte
2013
| Author's details | von Isabelle Erler. Mit Ill. von Igor Lange |
|---|---|
| Keywords | Schiffsreise ; Konfliktbewältigung ; Meereis ; Weihnachten |
| Language | German |
| Size | [14] Bl., zahlr. Ill., 193 mm x 170 mm |
| Publisher | Carlsen |
| Publishing place | Hamburg |
| Document type | Book |
| ISBN | 3551518017 ; 9783551518019 |
| Database | Former special subject collection: coastal and deep sea fishing |
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The Journal of biological chemistry
2006 Volume 281, Issue 50, Page(s) 38396–38404
Abstract: TRPM (transient receptor potential melastatin-like) channels are distinct from many other members of the transient receptor potential family in regard to their overall size (>1000 amino acids), the lack of N-terminal ankyrin-like repeats, and ... ...
| Abstract | TRPM (transient receptor potential melastatin-like) channels are distinct from many other members of the transient receptor potential family in regard to their overall size (>1000 amino acids), the lack of N-terminal ankyrin-like repeats, and hydrophobicity predictions that may allow for more than six transmembrane regions. Common to each TRPM member is a prominent C-terminal coiled coil region. Here we have shown that TRPM8 channels assemble as multimers using the putative coiled coil region within the intracellular C terminus and that this assembly can be disturbed by a single point mutation within the coiled coil region. This mutant neither gives rise to functional channels nor do its subunits interact or form protein complexes that correspond to a multimer. However, they are still transported to the plasma membrane. Furthermore, wild-type currents can be suppressed by expressing the membrane-attached C-terminal region of TRPM8. To separate assembly from trafficking, we investigated the maturation of TRPM8 protein by identifying and mutating the relevant N-linked glycosylation site and showing that glycosylation is neither essential for multimerization nor for transport to the plasma membrane per se but appears to facilitate efficient multimerization and transport. |
|---|---|
| MeSH term(s) | Animals ; Cell Line ; Cold Temperature ; Humans ; Immunoprecipitation ; Mutagenesis ; Mutagenesis, Site-Directed ; Protein Transport ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism ; Two-Hybrid System Techniques |
| Chemical Substances | Recombinant Proteins ; TRPM Cation Channels ; TRPM8 protein, human |
| Language | English |
| Publishing date | 2006-10-25 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2997-x |
| ISSN | 1083-351X ; 0021-9258 |
| ISSN (online) | 1083-351X |
| ISSN | 0021-9258 |
| DOI | 10.1074/jbc.M607756200 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Uc I Zs.108: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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(Pixi-Wissen ; 25)
2009
| Author's details | einfach gut erkl. von Isabelle Erler. Mit Bildern von Friederike Rave |
|---|---|
| Series title | Pixi-Wissen ; 25 |
| Keywords | Körper |
| Language | German |
| Size | 29 S., zahlr. Ill. und graph. Darst., 16 cm |
| Publisher | Carlsen |
| Publishing place | Hamburg |
| Document type | Book |
| Note | Hier auch später erschienene, unveränd. Nachdr. |
| ISBN | 9783551240750 ; 3551240752 |
| Database | Former special subject collection: coastal and deep sea fishing |
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Spiele, Rätsel, Tore
2010
| Author's details | [Text: Isabelle Erler. Illustrationen von Sigrid Leberer] |
|---|---|
| Size | [circa 96 Blatt]: zahlreiche Illustrationen |
| Publisher | Carlsen; Hamburg |
| Document type | Book |
| HBZ-ID | HT016002582 |
| ISBN | 978-3-551-18581-5 ; 3-551-18581-6 |
| Database | Central Library of Sport Science of the German Sport University Cologne |
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International journal of radiation oncology, biology, physics
2017 Volume 98, Issue 2, Page(s) 419–427
Abstract: Purpose: To assess the association between colorectal cancer (CRC) histology, dose, and local failure (LF) after stereotactic ablative radiation therapy (SABR) for pulmonary metastases, and to describe subsequent cancer progression, change of systemic ... ...
| Abstract | Purpose: To assess the association between colorectal cancer (CRC) histology, dose, and local failure (LF) after stereotactic ablative radiation therapy (SABR) for pulmonary metastases, and to describe subsequent cancer progression, change of systemic therapy (CST), survival, and their association with treatment indications. Methods and materials: From a prospective SABR cohort, 180 pulmonary metastases in 120 patients were identified. Treatment indications were single metastasis, oligometastases, oligoprogression, and dominant areas of progression. Doses of 48 to 52 Gy/4 to 5 fractions were delivered. Since 2010 the dose for peripheral CRC metastases was increased to 60 Gy/4 fractions. Cumulative incidence function (CIF) was used to report LF, progression probability, and CST. The Kaplan-Meier method estimated overall survival (OS). Univariate and multivariable analyses to assess variable associations were conducted. Results: Median follow-up was 22 months (interquartile range, 14-33 months). At 24 months, the CIF of LF was 23.6% (95% confidence interval [CI] 15.1%-33.3%) and 8.3% (95% CI 2.6%-18.6%), respectively, for CRC and non-CRC metastases (P<.001). This association remained significant after adjusting for confounders (subdistribution hazard ratio [SHR] 13.6, 95% CI 4.2-44.1, P<.001). Among CRC metastases, 56 and 45 received <60 Gy and 60 Gy, respectively. Delivering 60 Gy was independently associated with a lower hazard of LF (SHR 0.271, 95% CI 0.078-0.940, P=.040). At 12 months the CIF of progression was 41.67% (95% CI 21.69%-60.56%), 42.51% (95% CI 29.09%-55.29%), 62.96% (95% CI 41.25%-78.53%), and 78.57% (95% CI 42.20%-93.48%), respectively, for patients treated for single metastasis, oligometastases, oligoprogression, and dominant area of progression (P<.001). A CST was observed, respectively, in 4 (17%), 17 (31%), 12 (44%), and 10 (71%) patients with a median time of 13.1, 11.1, 8.4, and 8.4 months. Conclusion: Colorectal cancer lung metastases are associated with a higher hazard of LF and require higher SABR doses. Outcomes for patients with oligometastases and oligoprogression treated with SABR seem favorable. Prospective clinical trials are needed to confirm these benefits. |
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| MeSH term(s) | Aged ; Analysis of Variance ; Colorectal Neoplasms/pathology ; Disease Progression ; Dose Fractionation ; Female ; Follow-Up Studies ; Four-Dimensional Computed Tomography ; Humans ; Kaplan-Meier Estimate ; Lung Neoplasms/diagnostic imaging ; Lung Neoplasms/mortality ; Lung Neoplasms/radiotherapy ; Lung Neoplasms/secondary ; Male ; Probability ; Radiosurgery/adverse effects ; Radiosurgery/methods ; Radiotherapy Planning, Computer-Assisted/methods ; Time Factors ; Treatment Failure |
| Language | English |
| Publishing date | 2017-06-01 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 197614-x |
| ISSN | 1879-355X ; 0360-3016 |
| ISSN (online) | 1879-355X |
| ISSN | 0360-3016 |
| DOI | 10.1016/j.ijrobp.2017.02.093 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 1218: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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