LIVIVO - Search results -

Search results

Result 1 - 10 of total 3109

Search options

Article ; Online: Catch per Unit Effort of Decapod Species, C. pagurus and H. gammarus, from a Voluntary Marine Reserve

Easton, Blair Alexander Andrew / Scott, Kevin / Richards, Joe / Rees, Adam

Fishes. 2023 July 27, v. 8, no. 8

2023

Abstract: C. pagurus and H. gammarus are deemed to be declining in abundance in the Berwickshire Marine ... these decapod species. In this study, CPUE was higher for H. gammarus and C. pagurus outside the Berwickshire ... variables and showed that soak time (days) and depth (m) significantly affected CPUE for C. pagurus, not H ...

Abstract	C. pagurus and H. gammarus are deemed to be declining in abundance in the Berwickshire Marine Reserve from personal communications with local inshore fishers. Fisheries data in the form of catch per unit effort (CPUE) were collected for these two commercially important decapods. Other explanatory variables from fishing activity such as the creel and bait type used, the soak time of the fishing gear, and deployment depth were recorded to provide as much detail as possible to describe the effort applied to catch these decapod species. In this study, CPUE was higher for H. gammarus and C. pagurus outside the Berwickshire Marine Reserve. General additive models (GAMs) were used to describe the effects of the explanatory variables and showed that soak time (days) and depth (m) significantly affected CPUE for C. pagurus, not H. gammarus. Sea temperature (°C) showed a negative correlation with the CPUE of both H. gammarus and C. pagurus; however, a positive correlation was found with the number of C. pagurus caught. The data collected in this study provide a foundation in understanding the current abundance of C. pagurus and H. gammarus in a voluntary marine reserve on the east coast of Scotland, which can be used to inform future changes in fisheries management in Berwickshire.
Keywords	Decapoda ; additives ; aquacultural and fisheries equipment ; fish ; fisheries ; fisheries statistics ; models ; soaking ; temperature
Language	English
Dates of publication	2023-0727
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ISSN	2410-3888
DOI	10.3390/fishes8080390
Database	NAL-Catalogue (AGRICOLA)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Magnesium-mediated benzothiazole activation: a room-temperature cascade of C-H deprotonation, C-C coupling, ring-opening, and nucleophilic addition reactions.

Blair, Victoria L / Clegg, William / Kennedy, Alan R / Livingstone, Zoe / Russo, Luca / Hevia, Eva

Angewandte Chemie (International ed. in English)

2011 Volume 50, Issue 42, Page(s) 9857–9860

Abstract: ... magnesiation initiates an unstoppable domino reaction of C-C coupling, ring opening, nucleophilic addition, and ...

Abstract	Ligand domin(o)ated: In contrast to the straightforward deprotonation of benzothiazole using Grignard reagents, treatment of benzothiazole with 1 leads to a novel type of activation. The initial magnesiation initiates an unstoppable domino reaction of C-C coupling, ring opening, nucleophilic addition, and deprotonation to give 2. THF=tetrahydrofuran.
MeSH term(s)	Benzothiazoles/chemistry ; Crystallography, X-Ray ; Magnesium/chemistry ; Models, Molecular ; Molecular Structure ; Organometallic Compounds/chemical synthesis ; Organometallic Compounds/chemistry ; Protons ; Temperature
Chemical Substances	Benzothiazoles ; Organometallic Compounds ; Protons ; benzothiazole (G5BW2593EP) ; Magnesium (I38ZP9992A)
Language	English
Publishing date	2011-10-10
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.201103275
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Tumors induced in mice by direct inoculation of plasmid DNA expressing both activated H-ras and c-myc.

Sheng-Fowler, Li / Cai, Fang / Fu, Haiqing / Zhu, Yong / Orrison, Brian / Foseh, Gideon / Blair, Don G / Hughes, Stephen H / Coffin, John M / Lewis, Andrew M / Peden, Keith

International journal of biological sciences

2010 Volume 6, Issue 2, Page(s) 151–162

Abstract: ... human activated T24-H-ras and murine c-myc--and had shown that these two plasmids, pMSV-T24-H-ras and pMSV-c-myc ... tested. The dual-expression plasmid, pMSV-T24-H-ras/MSV-c-myc, is about 20-fold more efficient at tumor ... increased the efficiency of tumor induction. Based on these data, the dual-expression plasmid pMSV-T24-H-ras/MSV-c-myc ...

Abstract	Vaccines contain residual DNA derived from the cells used to produce them. As part of our investigation to assess the risk of this cellular DNA, we are developing a quantitative in vivo assay to assess the oncogenicity of DNA. In an earlier study, we had generated expression plasmids for two oncogenes--human activated T24-H-ras and murine c-myc--and had shown that these two plasmids, pMSV-T24-H-ras and pMSV-c-myc, could act in concert to induce tumors in mice, although the efficiency was low. In this study, we took two approaches to increase the oncogenic efficiency: 1) both oncogene-expression cassettes were placed on the same plasmid; 2) transfection facilitators, which increase DNA uptake and expression in vitro, were tested. The dual-expression plasmid, pMSV-T24-H-ras/MSV-c-myc, is about 20-fold more efficient at tumor induction in newborn NIH Swiss mice than the separate expression plasmids, with tumors being induced with 1 microg of the dual-expression plasmid DNA. However, none of the transfection facilitators tested increased the efficiency of tumor induction. Based on these data, the dual-expression plasmid pMSV-T24-H-ras/MSV-c-myc will be used as the positive control to develop a sensitive and quantitative animal assay that can be used to assess the oncogenic activity of DNA.
MeSH term(s)	Animals ; DNA/metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Mice ; Mice, Inbred C57BL ; NIH 3T3 Cells ; Neoplasms/metabolism ; Oncogenes ; Plasmids/metabolism ; Polymerase Chain Reaction/methods ; Proto-Oncogene Proteins c-myc/metabolism ; Rats ; Transfection ; ras Proteins/metabolism
Chemical Substances	Proto-Oncogene Proteins c-myc ; DNA (9007-49-2) ; ras Proteins (EC 3.6.5.2)
Language	English
Publishing date	2010-03-29
Publishing country	Australia
Document type	Journal Article ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2179208-2
ISSN	1449-2288 ; 1449-2288
ISSN (online)	1449-2288
ISSN	1449-2288
DOI	10.7150/ijbs.6.151
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A mouse strain defective in both T cells and NK cells has enhanced sensitivity to tumor induction by plasmid DNA expressing both activated H-Ras and c-Myc.

Sheng-Fowler, Li / Tu, Wei / Fu, Haiqing / Murata, Haruhiko / Lanning, Lynda / Foseh, Gideon / Macauley, Juliete / Blair, Donald / Hughes, Stephen H / Coffin, John M / Lewis, Andrew M / Peden, Keith

PloS one

2014 Volume 9, Issue 10, Page(s) e108926

Abstract: ... We generated a plasmid expressing both an activated human H-ras gene and murine c-myc gene and showed that 1 µg ... of this plasmid, pMSV-T24-H-ras/MSV-c-myc, was capable of inducing tumors in newborn NIH Swiss mice ... form of pMSV-T24-H-ras/MSV-c-myc. When this plasmid was inoculated as linear DNA, amounts of DNA as low ...

Abstract	As part of safety studies to evaluate the risk of residual cellular DNA in vaccines manufactured in tumorigenic cells, we have been developing in vivo assays to detect and quantify the oncogenic activity of DNA. We generated a plasmid expressing both an activated human H-ras gene and murine c-myc gene and showed that 1 µg of this plasmid, pMSV-T24-H-ras/MSV-c-myc, was capable of inducing tumors in newborn NIH Swiss mice. However, to be able to detect the oncogenicity of dominant activated oncogenes in cellular DNA, a more sensitive system was needed. In this paper, we demonstrate that the newborn CD3 epsilon transgenic mouse, which is defective in both T-cell and NK-cell functions, can detect the oncogenic activity of 25 ng of the circular form of pMSV-T24-H-ras/MSV-c-myc. When this plasmid was inoculated as linear DNA, amounts of DNA as low as 800 pg were capable of inducing tumors. Animals were found that had multiple tumors, and these tumors were independent and likely clonal. These results demonstrate that the newborn CD3 epsilon mouse is highly sensitive for the detection of oncogenic activity of DNA. To determine whether it can detect the oncogenic activity of cellular DNA derived from four human tumor-cell lines (HeLa, A549, HT-1080, and CEM), DNA (100 µg) was inoculated into newborn CD3 epsilon mice both in the presence of 1 µg of linear pMSV-T24-H-ras/MSV-c-myc as positive control and in its absence. While tumors were induced in 100% of mice with the positive-control plasmid, no tumors were induced in mice receiving any of the tumor DNAs alone. These results demonstrate that detection of oncogenes in cellular DNA derived from four human tumor-derived cell lines in this mouse system was not possible; the results also show the importance of including a positive-control plasmid to detect inhibitory effects of the cellular DNA.
MeSH term(s)	Animals ; Cell Line, Tumor ; DNA, Circular/genetics ; DNA, Neoplasm/genetics ; Genes, myc ; Genes, ras ; Humans ; Killer Cells, Natural/metabolism ; Killer Cells, Natural/pathology ; Mice/genetics ; Mice/physiology ; Mice, Transgenic ; Neoplasms/genetics ; Neoplasms/pathology ; Plasmids/genetics ; T-Lymphocytes/metabolism ; T-Lymphocytes/pathology
Chemical Substances	DNA, Circular ; DNA, Neoplasm
Language	English
Publishing date	2014-10-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0108926
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Oncogenicity of DNA in vivo: tumor induction with expression plasmids for activated H-ras and c-myc.

Sheng, Li / Cai, Fang / Zhu, Yong / Pal, Achintya / Athanasiou, Meropi / Orrison, Brian / Blair, Donald G / Hughes, Stephen H / Coffin, John M / Lewis, Andrew M / Peden, Keith

Biologicals : journal of the International Association of Biological Standardization

2008 Volume 36, Issue 3, Page(s) 184–197

Abstract: ... inoculated with different amounts of either plasmid alone or with a combination of the H-ras and c-myc ... step in resolving this issue, we have generated expression plasmids for the activated human H ... ras oncogene and for the murine c-myc proto-oncogene. Their oncogenic activity was confirmed in vitro using ...

Abstract	All vaccines and other biological products contain contaminating residual DNA derived from the production cell substrate. Whether this residual cell-substrate DNA can induce tumors in vaccine recipients and thus represent a risk factor has been debated for over 50 years without resolution. As a first step in resolving this issue, we have generated expression plasmids for the activated human H-ras oncogene and for the murine c-myc proto-oncogene. Their oncogenic activity was confirmed in vitro using the focus-formation transformation assay. Two strains of adult and newborn immune-competent mice were inoculated with different amounts of either plasmid alone or with a combination of the H-ras and c-myc plasmids. Tumors developed only in mice inoculated with both plasmids and only at the highest amount of DNA (12.5 microg of each plasmid). The NIH Swiss mouse was more sensitive than the C57BL/6 mouse, and newborn animals were more sensitive than adults. Cell lines were established from the tumors. PCR and Southern hybridization analyses demonstrated that both inoculated oncogenes were present in all of the tumor-derived cell lines and that the cells in the tumors were clonal. Western analysis demonstrated that both oncoproteins were expressed in these cell lines. These results demonstrate that cellular oncogenes can induce tumors following subcutaneous inoculation. Such information provides a possible way of evaluating and estimating the theoretical oncogenic risk posed by residual cell-substrate DNA in vaccines.
MeSH term(s)	Animals ; Cancer Vaccines/metabolism ; DNA/chemistry ; DNA/metabolism ; Mice ; Mice, Inbred C57BL ; Models, Biological ; NIH 3T3 Cells ; Neoplasm Transplantation ; Neoplasms/metabolism ; Oligonucleotides/chemistry ; Plasmids/metabolism ; Proto-Oncogene Proteins c-myc/metabolism ; Rats ; Risk Factors ; ras Proteins/metabolism
Chemical Substances	Cancer Vaccines ; Oligonucleotides ; Proto-Oncogene Proteins c-myc ; DNA (9007-49-2) ; ras Proteins (EC 3.6.5.2)
Language	English
Publishing date	2008-01-22
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	1017370-5
ISSN	1095-8320 ; 1045-1056
ISSN (online)	1095-8320
ISSN	1045-1056
DOI	10.1016/j.biologicals.2007.11.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 879: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Tumors Induced in Mice by Direct Inoculation of Plasmid DNA Expressing Both Activated H-ras and c-myc

Li Sheng-Fowler, Fang Cai, Haiqing Fu, Yong Zhu, Brian Orrison, Gideon Foseh, Don G. Blair, Stephen H. Hughes, John M. Coffin, Andrew M. Lewis Jr, Keith Peden

International Journal of Biological Sciences, Vol 6, Iss 2, Pp 151-

2010 Volume 162

Abstract: ... human activated T24-H- ras and murine c- myc - and had shown that these two plasmids, pMSV-T24-H- ras and pMSV-c ... in vitro, were tested. The dual-expression plasmid, pMSV-T24-H- ras /MSV-c- myc , is about 20-fold more ... plasmid pMSV-T24-H- ras /MSV-c- myc will be used as the positive control to develop a sensitive and ...

Abstract	Vaccines contain residual DNA derived from the cells used to produce them. As part of our investigation to assess the risk of this cellular DNA, we are developing a quantitative in vivo assay to assess the oncogenicity of DNA. In an earlier study, we had generated expression plasmids for two oncogenes - human activated T24-H- ras and murine c- myc - and had shown that these two plasmids, pMSV-T24-H- ras and pMSV-c- myc , could act in concert to induce tumors in mice, although the efficiency was low. In this study, we took two approaches to increase the oncogenic efficiency: 1) both oncogene-expression cassettes were placed on the same plasmid; 2) transfection facilitators, which increase DNA uptake and expression in vitro, were tested. The dual-expression plasmid, pMSV-T24-H- ras /MSV-c- myc , is about 20-fold more efficient at tumor induction in newborn NIH Swiss mice than the separate expression plasmids, with tumors being induced with 1 µg of the dual-expression plasmid DNA. However, none of the transfection facilitators tested increased the efficiency of tumor induction. Based on these data, the dual-expression plasmid pMSV-T24-H- ras /MSV-c- myc will be used as the positive control to develop a sensitive and quantitative animal assay that can be used to assess the oncogenic activity of DNA.
Keywords	Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2010-01-01T00:00:00Z
Publisher	Ivyspring International Publisher
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A mouse strain defective in both T cells and NK cells has enhanced sensitivity to tumor induction by plasmid DNA expressing both activated H-Ras and c-Myc.

Li Sheng-Fowler / Wei Tu / Haiqing Fu / Haruhiko Murata / Lynda Lanning / Gideon Foseh / Juliete Macauley / Donald Blair / Stephen H Hughes / John M Coffin / Andrew M Lewis / Keith Peden

PLoS ONE, Vol 9, Iss 10, p e

2014 Volume 108926

Abstract	As part of safety studies to evaluate the risk of residual cellular DNA in vaccines manufactured in tumorigenic cells, we have been developing in vivo assays to detect and quantify the oncogenic activity of DNA. We generated a plasmid expressing both an activated human H-ras gene and murine c-myc gene and showed that 1 µg of this plasmid, pMSV-T24-H-ras/MSV-c-myc, was capable of inducing tumors in newborn NIH Swiss mice. However, to be able to detect the oncogenicity of dominant activated oncogenes in cellular DNA, a more sensitive system was needed. In this paper, we demonstrate that the newborn CD3 epsilon transgenic mouse, which is defective in both T-cell and NK-cell functions, can detect the oncogenic activity of 25 ng of the circular form of pMSV-T24-H-ras/MSV-c-myc. When this plasmid was inoculated as linear DNA, amounts of DNA as low as 800 pg were capable of inducing tumors. Animals were found that had multiple tumors, and these tumors were independent and likely clonal. These results demonstrate that the newborn CD3 epsilon mouse is highly sensitive for the detection of oncogenic activity of DNA. To determine whether it can detect the oncogenic activity of cellular DNA derived from four human tumor-cell lines (HeLa, A549, HT-1080, and CEM), DNA (100 µg) was inoculated into newborn CD3 epsilon mice both in the presence of 1 µg of linear pMSV-T24-H-ras/MSV-c-myc as positive control and in its absence. While tumors were induced in 100% of mice with the positive-control plasmid, no tumors were induced in mice receiving any of the tumor DNAs alone. These results demonstrate that detection of oncogenes in cellular DNA derived from four human tumor-derived cell lines in this mouse system was not possible; the results also show the importance of including a positive-control plasmid to detect inhibitory effects of the cellular DNA.
Keywords	Medicine ; R ; Science ; Q
Subject code	500
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: 1993 C.H. McCloy Research Lecture: physical activity, physical fitness, and health.

Blair, S N

Research quarterly for exercise and sport

1993 Volume 64, Issue 4, Page(s) 365–376

MeSH term(s)	Epidemiology ; Female ; Health ; Humans ; Male ; Mortality ; Motor Activity/physiology ; Physical Fitness/physiology ; Preventive Medicine
Language	English
Publishing date	1993-12
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S. ; Review
ZDB-ID	225654-x
ISSN	2168-3824 ; 0270-1367
ISSN (online)	2168-3824
ISSN	0270-1367
DOI	10.1080/02701367.1993.10607589
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 58: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A homologous series of alkaline earth phosphanides: syntheses, crystal structures, and unusual dynamic behavior of (THF)(n)M[P(CH(SiMe(3))(2))(C(6)H(4)-2-CH(2)NMe(2))](2) (M = Mg, Ca, Sr, Ba).

Blair, Stuart / Izod, Keith / Clegg, William

Inorganic chemistry

2002 Volume 41, Issue 15, Page(s) 3886–3893

Abstract: The secondary phosphine R(Me(2)NCH(2)-2-C(6)H(4))PH reacts with Bu(2)Mg to give the homoleptic ... complex Mg[PR(C(6)H(4)-2-CH(2)NMe(2))](2) (1) [R = CH(SiMe(3))(2)]. The analogous heavier ... alkaline earth metal complexes (THF)(n)Ae[PR(C(6)H(4)-2-CH(2)NMe(2))](2) [Ae = Ca (2), n = 0; Ae = Sr (3), Ba (4), n ...

Abstract	The secondary phosphine R(Me(2)NCH(2)-2-C(6)H(4))PH reacts with Bu(2)Mg to give the homoleptic complex Mg[PR(C(6)H(4)-2-CH(2)NMe(2))](2) (1) [R = CH(SiMe(3))(2)]. The analogous heavier alkaline earth metal complexes (THF)(n)Ae[PR(C(6)H(4)-2-CH(2)NMe(2))](2) [Ae = Ca (2), n = 0; Ae = Sr (3), Ba (4), n = 1] have been synthesized by metathesis reactions between K[PR(C(6)H(4)-2-CH(2)NMe(2))] and 0.5 equiv of the respective alkaline earth metal diiodide. Compounds 1-4 have been characterized by X-ray crystallography and multielement NMR spectroscopy. In the solid state, compounds 1-4 are monomeric, complexes 1 and 2 adopting a distorted tetrahedral geometry and complexes 3 and 4 adopting a distorted square pyramidal geometry (1: orthorhombic, P2(1)2(1)2(1), a = 11.413(3) A, b = 12.072(3) A, c = 32.620(11) A, Z = 4. 2: monoclinic, P2(1)/c, a = 9.5550(4) A, b = 17.4560(7) A, c = 24.5782(10) A, beta = 91.673(2) degrees, Z = 4. 3: monoclinic, C2/c, a = 15.0498(9) A, b = 13.0180(8) A, c = 24.3664(14) A, beta = 104.593(2) degrees, Z = 4. 4: monoclinic, C2/c, a = 15.2930(10) A, b = 13.0326(9) A, c = 24.6491(17) A, beta = 105.542(2) degrees, Z = 4). In toluene solution, compounds 2-4 are subject to dynamic processes which are attributed to a monomer-dimer equilibrium for which bridge-terminal exchange of the phosphanide ligands in the dimer may be frozen out at low temperatures.
Language	English
Publishing date	2002-07-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	1484438-2
ISSN	1520-510X ; 0020-1669
ISSN (online)	1520-510X
ISSN	0020-1669
DOI	10.1021/ic0201194
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The ecto-nucleotide pyrophosphatase/phosphodiesterase 2 promotes early osteoblast differentiation and mineralization in stromal stem cells.

Tourkova, Irina L / Larrouture, Quitterie C / Liu, Silvia / Luo, Jianhua / Schlesinger, Paul H / Blair, Harry C

American journal of physiology. Cell physiology

2024 Volume 326, Issue 3, Page(s) C843–C849

Abstract: The phosphodiesterase enzymes mediate calcium-phosphate deposition in various tissues, although which enzymes are active in bone mineralization is unclear. Using gene array analysis, we found that a member of ecto-nucleotide pyrophosphatase/ ... ...

Abstract	The phosphodiesterase enzymes mediate calcium-phosphate deposition in various tissues, although which enzymes are active in bone mineralization is unclear. Using gene array analysis, we found that a member of ecto-nucleotide pyrophosphatase/phosphodiesterase family, ENPP2, was strongly down-regulated with age in stromal stem cells that produce osteoblasts and make bone. This is in keeping with reduced bone formation in older animals. Thus, we hypothesized that ENPP2 is, at least in part, an early mediator of bone formation and thus may reflect reduced bone formation with age. Since ENPP2 has not previously been shown to have a role in osteoblast differentiation, we studied its effect on bone differentiation from stromal stem cells, verified by flow cytometry for stem cell antigens. In these remarkably uniform osteoblast precursors, we did transfection with ENPP2 DsiRNA, scrambled DsiRNA, or no transfection to make cells with normal or greatly reduced ENPP2 and analyzed osteoblast differentiation and mineralization. Osteoblast differentiation down-regulation was shown by alizarin red binding, silver staining, and alkaline phosphatase activity. Differences were confirmed by real-time PCR for alkaline phosphatase (ALPL), osteocalcin (BGLAP), and ENPP2 and by Western Blot for Enpp2. These were decreased, ∼50%, in osteoblasts transfected with ENPP2 DsiRNA compared with cells transfected with a scrambled DsiRNA or not transfected (control) cells. This finding is the first evidence for the role of ENPP2 in osteoblast differentiation and mineralization.
MeSH term(s)	Animals ; Osteogenesis ; Alkaline Phosphatase/genetics ; Cell Differentiation ; Phosphoric Diester Hydrolases/genetics ; Calcinosis ; Pyrophosphatases
Chemical Substances	nucleotide pyrophosphatase (EC 3.6.1.9) ; Alkaline Phosphatase (EC 3.1.3.1) ; Phosphoric Diester Hydrolases (EC 3.1.4.-) ; Pyrophosphatases (EC 3.6.1.-)
Language	English
Publishing date	2024-01-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	392098-7
ISSN	1522-1563 ; 0363-6143
ISSN (online)	1522-1563
ISSN	0363-6143
DOI	10.1152/ajpcell.00692.2023
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.89: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito