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Article ; Online: Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells.

Sparkes, Imogen / White, Rhiannon R / Bateman, Benji / Botchway, Stanley / Ward, Andy

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2772, Page(s) 179–190

Abstract: Optical tweezers have been used to trap and micro-manipulate several biological specimens ranging from DNA, macromolecules, organelles, to single-celled organisms. Using a combination of the refraction and scattering of laser light from a focused laser ... ...

Abstract	Optical tweezers have been used to trap and micro-manipulate several biological specimens ranging from DNA, macromolecules, organelles, to single-celled organisms. Using a combination of the refraction and scattering of laser light from a focused laser beam, refractile objects are physically captured and can be moved within the surrounding media. The technique is routinely used to determine biophysical properties such as the forces exerted by motor proteins. Here, we describe how optical tweezers combined with total internal reflection fluorescence microscopy (TIRF) can be used to assess physical interactions between organelles, more specifically the ER and Golgi bodies in plant cells.
MeSH term(s)	Microscopy ; Optical Tweezers ; Plant Cells ; Golgi Apparatus ; Biophysics
Language	English
Publishing date	2024-02-27
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3710-4_13
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Label-free complete absorption microscopy using second generation photoacoustic remote sensing.

Ecclestone, Benjamin R / Bell, Kevan / Sparkes, Sarah / Dinakaran, Deepak / Mackey, John R / Haji Reza, Parsin

Scientific reports

2022 Volume 12, Issue 1, Page(s) 8464

Abstract: In the past decades, absorption modalities have emerged as powerful tools for label-free functional and structural imaging of cells and tissues. Many biomolecules present unique absorption spectra providing chromophore-specific information on properties ... ...

Abstract	In the past decades, absorption modalities have emerged as powerful tools for label-free functional and structural imaging of cells and tissues. Many biomolecules present unique absorption spectra providing chromophore-specific information on properties such as chemical bonding, and sample composition. As chromophores absorb photons the absorbed energy is emitted as photons (radiative relaxation) or converted to heat and under specific conditions pressure (non-radiative relaxation). Modalities like fluorescence microscopy may capture radiative relaxation to provide contrast, while modalities like photoacoustic microscopy may leverage non-radiative heat and pressures. Here we show an all-optical non-contact total-absorption photoacoustic remote sensing (TA-PARS) microscope, which can capture both radiative and non-radiative absorption effects in a single acquisition. The TA-PARS yields an absorption metric proposed as the quantum efficiency ratio (QER), which visualizes a biomolecule's proportional radiative and non-radiative absorption response. The TA-PARS provides label-free visualization of a range of biomolecules enabling convincing analogues to traditional histochemical staining of tissues, effectively providing label-free Hematoxylin and Eosin (H&E)-like visualizations. These findings establish an effective all-optical non-contact total-absorption microscope for label-free inspection of biological materials.
MeSH term(s)	Eosine Yellowish-(YS) ; Hematoxylin ; Microscopy, Fluorescence ; Photoacoustic Techniques/methods ; Remote Sensing Technology/methods
Chemical Substances	Eosine Yellowish-(YS) (TDQ283MPCW) ; Hematoxylin (YKM8PY2Z55)
Language	English
Publishing date	2022-05-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-11235-3
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Article ; Online: Impact of Steroid Only Induction on Rejection in Simultaneous Liver-Kidney Transplantation.

Ruiz, Irene / Sparkes, Tracy / Masters, Brian / Barth, Rolf / Maluf, Daniel / Freedman, Sari

Progress in transplantation (Aliso Viejo, Calif.)

2022 Volume 32, Issue 4, Page(s) 363–369

Abstract: Introduction: ...

Abstract	Introduction:
MeSH term(s)	Adult ; Humans ; Kidney Transplantation ; Basiliximab ; Immunosuppressive Agents/therapeutic use ; Graft Rejection/prevention & control ; Retrospective Studies ; Cohort Studies ; Antibodies, Monoclonal/therapeutic use ; Mycophenolic Acid/therapeutic use ; Tacrolimus/therapeutic use ; Steroids ; Adrenal Cortex Hormones ; Kidney ; Liver
Chemical Substances	Basiliximab (9927MT646M) ; Immunosuppressive Agents ; Antibodies, Monoclonal ; Mycophenolic Acid (HU9DX48N0T) ; Tacrolimus (WM0HAQ4WNM) ; Steroids ; Adrenal Cortex Hormones
Language	English
Publishing date	2022-09-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2864264-8
ISSN	2164-6708 ; 1526-9248
ISSN (online)	2164-6708
ISSN	1526-9248
DOI	10.1177/15269248221122883
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Article: Genetic counseling.

Sparkes, R S

California medicine

2008 Volume 119, Issue 1, Page(s) 62

Language	English
Publishing date	2008-08-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	390024-1
ISSN	0008-1264 ; 0093-4038
ISSN	0008-1264 ; 0093-4038
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Article: Kombucha tea as an anti-hyperglycemic agent in humans with diabetes - a randomized controlled pilot investigation.

Mendelson, Chagai / Sparkes, Sabrina / Merenstein, Daniel J / Christensen, Chloe / Sharma, Varun / Desale, Sameer / Auchtung, Jennifer M / Kok, Car Reen / Hallen-Adams, Heather E / Hutkins, Robert

Frontiers in nutrition

2023 Volume 10, Page(s) 1190248

Abstract: Introduction: Kombucha is a popular fermented tea that has attracted considerable attention due, in part, to its suggested health benefits. Previous results from animal models led us to hypothesize kombucha may reduce blood sugar levels in humans with ... ...

Abstract	Introduction: Kombucha is a popular fermented tea that has attracted considerable attention due, in part, to its suggested health benefits. Previous results from animal models led us to hypothesize kombucha may reduce blood sugar levels in humans with diabetes. The objective of this pilot clinical study was to evaluate kombucha for its anti-hyperglycemic activities in adults with diabetes mellitus type II. Methods: The study was organized as a prospective randomized double-blinded crossover study at a single-center urban hospital system. Participants ( Results: Kombucha lowered average fasting blood glucose levels at 4 weeks compared to baseline (164 vs. 116 mg/dL, Discussion: Although this pilot study was limited by a small sample size, kombucha was associated with reduced blood glucose levels in humans with diabetes. Larger follow-up studies are warranted. Clinical trial registration: ClinicalTrials.gov, identifier NCT04107207.
Language	English
Publishing date	2023-08-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2776676-7
ISSN	2296-861X
ISSN	2296-861X
DOI	10.3389/fnut.2023.1190248
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Article ; Online: Priorities for peer support delivery among adults living with chronic kidney disease: a patient-oriented consensus workshop.

Elliott, Meghan J / Donald, Maoliosa / Farragher, Janine / Verdin, Nancy / Love, Shannan / Manns, Kate / Baragar, Brigitte / Sparkes, Dwight / Fox, Danielle / Hemmelgarn, Brenda R

CMAJ open

2023 Volume 11, Issue 4, Page(s) E736–E744

Abstract: Background: Peer support can address the informational and emotional needs of people living with chronic kidney disease (CKD) and enable self-management. We aimed to identify preferences and priorities for content, format and processes of peer support ... ...

Abstract	Background: Peer support can address the informational and emotional needs of people living with chronic kidney disease (CKD) and enable self-management. We aimed to identify preferences and priorities for content, format and processes of peer support delivery for patients with non-dialysis CKD and their loved ones. Methods: Using a patient-oriented research approach, we conducted a half-day, virtual consensus workshop with stakeholder participants from across Canada, including patients, caregivers, peer mentors and clinicians. Using personas (fictional characters), participants discussed and voted on preferences for delivery of peer support across format, content and process categories. We analyzed transcripts from small- and large-group discussions inductively using content analysis. Results: Twenty-one stakeholders, including 9 patients and 4 caregivers, participated in the workshop. In the voting exercise on format, participants prioritized peer mentor matching, programming for both patients and caregivers, and flexible scheduling. For content, participants prioritized informational and emotional support focus, and for process, they prioritized leveraging kidney care programs and alternative sources (e.g., social media) for promotion and referral. Analysis of workshop transcripts complemented prioritization results and emphasized tailoring of peer support delivery to accommodate the diversity of people living with CKD and their support needs. This concept was elaborated in 3 themes, namely alignment of program features with needs, inclusive peer support options and multiple access points. Interpretation: We identified preferences for peer support delivery for people living with CKD and underscore the importance of tailored, flexible programming in this context. Findings could be used to develop, adapt or study CKD-focused peer support interventions.
Language	English
Publishing date	2023-08-15
Publishing country	Canada
Document type	Journal Article
ZDB-ID	2701622-5
ISSN	2291-0026 ; 2291-0026
ISSN (online)	2291-0026
ISSN	2291-0026
DOI	10.9778/cmajo.20220171
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Article ; Online: Survival benefit of renal transplantation in octogenarians.

Ravichandran, Bharath R / Sparkes, Tracy M / Masters, Brian M / Thomas, Beje / Demehin, Moses / Bromberg, Jonathan S / Haririan, Abdolreza

Clinical transplantation

2020 Volume 34, Issue 11, Page(s) e14074

Abstract: Background: Elderly patients are the fastest growing population requiring renal replacement therapy. As previous studies have shown a survival benefit of kidney transplantation compared to dialysis for end-stage renal disease, we sought to evaluate if ... ...

Abstract	Background: Elderly patients are the fastest growing population requiring renal replacement therapy. As previous studies have shown a survival benefit of kidney transplantation compared to dialysis for end-stage renal disease, we sought to evaluate if this survival benefit extends to octogenarians. Methods: This was a single-center retrospective cohort study of renal allograft recipients ≥80 years transplanted from 1999 to 2014 who were compared to patients listed during the same period that did not proceed to transplantation. A secondary matched group was selected from the UNOS transplant waitlist database. The primary outcome was patient survival. Secondary outcomes included graft survival and rejection incidence. Results: Thirty-three transplanted patients were compared to 71 patients waitlisted at our center and 66 patients from the UNOS database. Patients in the study group were transplanted 20.8 ± 16.1 months after listing. Patient survival was 87.8% at 6 months and 1 year and 71.4% at 3 years. Kidney transplantation was associated with a significant decrease in the risk of death after listing (HR: 0.22, CI: 0.11-0.45, P < .001). Conclusion: With escalating life expectancy, kidney transplantation is a suitable treatment option in eligible octogenarians.
MeSH term(s)	Aged ; Aged, 80 and over ; Graft Rejection/etiology ; Graft Survival ; Humans ; Kidney Failure, Chronic/surgery ; Kidney Transplantation ; Renal Dialysis ; Retrospective Studies ; Survival Rate
Language	English
Publishing date	2020-09-27
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	639001-8
ISSN	1399-0012 ; 0902-0063
ISSN (online)	1399-0012
ISSN	0902-0063
DOI	10.1111/ctr.14074
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Zs.A 2204: Show issues

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Article ; Online: 3-Hydroxyisobutyric acid dehydrogenase deficiency: Expanding the clinical spectrum and quantitation of D- and L-3-Hydroxyisobutyric acid by an LC-MS/MS method.

Sasarman, Florin / Ferdinandusse, Sacha / Sinasac, David S / Fung, Ernest / Sparkes, Rebecca / Reeves, Melanie / Rombough, Catherine / Sass, Jörn Oliver / Voit, Renate / Ruiter, Jos P N / Koster, Janet / Waterham, Hans R / Pasquini, Elisabetta / Donati, Maria A / Marquardt, Thorsten / Wanders, Ronald J A / Al-Hertani, Walla

Journal of inherited metabolic disease

2022 Volume 45, Issue 3, Page(s) 445–455

Abstract: A deficiency of 3-hydroxyisobutyric acid dehydrogenase (HIBADH) has been recently identified as a cause of primary 3-hydroxyisobutyric aciduria in two siblings; the only previously recognized primary cause had been a deficiency of methylmalonic ... ...

Abstract	A deficiency of 3-hydroxyisobutyric acid dehydrogenase (HIBADH) has been recently identified as a cause of primary 3-hydroxyisobutyric aciduria in two siblings; the only previously recognized primary cause had been a deficiency of methylmalonic semialdehyde dehydrogenase, the enzyme that is immediately downstream of HIBADH in the valine catabolic pathway and is encoded by the ALDH6A1 gene. Here we report on three additional patients from two unrelated families who present with marked and persistent elevations of urine L-3-hydroxyisobutyric acid (L-3HIBA) and a range of clinical findings. Molecular genetic analyses revealed novel, homozygous variants in the HIBADH gene that are private within each family. Evidence for pathogenicity of the identified variants is presented, including enzymatic deficiency of HIBADH in patient fibroblasts. This report describes new variants in HIBADH as an underlying cause of primary 3-hydroxyisobutyric aciduria and expands the clinical spectrum of this recently identified inborn error of valine metabolism. Additionally, we describe a quantitative method for the measurement of D- and L-3HIBA in plasma and urine and present the results of a valine restriction therapy in one of the patients.
MeSH term(s)	Amino Acid Metabolism, Inborn Errors/metabolism ; Chromatography, Liquid ; Humans ; Hydroxybutyrates/urine ; Oxidoreductases ; Tandem Mass Spectrometry ; Valine
Chemical Substances	Hydroxybutyrates ; Oxidoreductases (EC 1.-) ; Valine (HG18B9YRS7) ; 3-hydroxyisobutyric acid (K75C8JDF5W)
Language	English
Publishing date	2022-03-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	438341-2
ISSN	1573-2665 ; 0141-8955
ISSN (online)	1573-2665
ISSN	0141-8955
DOI	10.1002/jimd.12486
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Zs.A 1508: Show issues

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Article ; Online: Defining Steric Requirements at CB

Markham, Jack / Sparkes, Eric / Boyd, Rochelle / Chen, Shuli / Manning, Jamie J / Finlay, David / Lai, Felcia / McGregor, Eila / Maloney, Callan J / Gerona, Roy R / Connor, Mark / McGregor, Iain S / Hibbs, David E / Glass, Michelle / Kevin, Richard C / Banister, Samuel D

ACS chemical neuroscience

2022 Volume 13, Issue 8, Page(s) 1281–1295

Abstract: Synthetic cannabinoid receptor agonists (SCRAs) are a diverse class of new psychoactive substances (NPS). They commonly ... ...

Abstract	Synthetic cannabinoid receptor agonists (SCRAs) are a diverse class of new psychoactive substances (NPS). They commonly comprise
MeSH term(s)	Animals ; Cannabinoid Receptor Agonists/chemistry ; Cannabinoid Receptor Agonists/pharmacology ; Cannabinoids/chemistry ; Central Nervous System Agents ; Indazoles/chemistry ; Indazoles/pharmacology ; Mice ; Receptor, Cannabinoid, CB1 ; Receptor, Cannabinoid, CB2 ; Receptors, Cannabinoid ; Valine/analogs & derivatives
Chemical Substances	5-F-ADB-PINACA ; Cannabinoid Receptor Agonists ; Cannabinoids ; Central Nervous System Agents ; Indazoles ; Receptor, Cannabinoid, CB1 ; Receptor, Cannabinoid, CB2 ; Receptors, Cannabinoid ; N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-(5-fluoropentyl)-1H-indazole-3-carboxamide (8FST4IAG3N) ; Valine (HG18B9YRS7)
Language	English
Publishing date	2022-04-11
Publishing country	United States
Document type	Journal Article
ISSN	1948-7193
ISSN (online)	1948-7193
DOI	10.1021/acschemneuro.2c00034
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Article ; Online: Simple and reliable protocol for identifying talented junior players in team sports using small-sided games.

Wilson, Robbie S / Hunter, Andrew H / Camata, Thiago V / Foster, Charles S P / Sparkes, Gabriella R / Moura, Felipe A / Santiago, Paulo R P / Smith, Nicholas M A

Scandinavian journal of medicine & science in sports

2021 Volume 31, Issue 8, Page(s) 1647–1656

Abstract: We designed and tested a protocol for measuring the performance of individuals in small-sided soccer games. We tested our protocol on three different groups of youth players from elite Brazilian football academies. Players in each group played a series ... ...

Abstract	We designed and tested a protocol for measuring the performance of individuals in small-sided soccer games. We tested our protocol on three different groups of youth players from elite Brazilian football academies. Players in each group played a series of 3v3 games, in which individuals were randomly assigned into new teams and against new opponents for each game. We calculated each individual's average individual goals scored, goals scored by teammates, goals conceded, and net team goals per game. Our protocol was consistent across days and repeatable across groups, with intraclass correlation coefficients (ICCs) of 0.57-0.69 for average net goals per game across testing days. Players could achieve high success by scoring goals or ensuring their team concede few goals. We also calculated the first and second dimension of a principal component analysis based on each player's number of goals scored, goals scored by teammates, and number of goals conceded per game. Players that were overall high performers had higher PC
MeSH term(s)	Aptitude ; Athletes/statistics & numerical data ; Athletic Performance/physiology ; Athletic Performance/statistics & numerical data ; Child ; Exercise Test ; Humans ; Reproducibility of Results ; Soccer/physiology ; Soccer/statistics & numerical data
Language	English
Publishing date	2021-04-24
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	1077418-x
ISSN	1600-0838 ; 0905-7188
ISSN (online)	1600-0838
ISSN	0905-7188
DOI	10.1111/sms.13969
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