LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 52

Search options

  1. Article ; Online: Assessing IgE sensitization profiles to birch and timothy grass pollen allergens in birch pollen allergic blood donors using an oligoplex specific IgE assay.

    Mahmood, Faiza / Nissen-Meyer, Lise Sofie Haug / Hetland, Geir / Nentwich, Ivo

    Scandinavian journal of clinical and laboratory investigation

    2023  Volume 83, Issue 7, Page(s) 444–447

    Abstract: IgE sensitization profiles to single birch allergens in birch-sensitized patients differ among European countries. The aim of this study was to determine the distribution of specific IgE antibodies to major and minor birch pollen allergens in a ... ...

    Abstract IgE sensitization profiles to single birch allergens in birch-sensitized patients differ among European countries. The aim of this study was to determine the distribution of specific IgE antibodies to major and minor birch pollen allergens in a population of allergic Norwegian individuals by using a birch allergic blood donor population as a surrogate sample. Sixty blood donors were recruited and sampled based on birch allergy symptoms such as rhinitis, rhinoconjunctivitis and/or mild asthma in previous seasons. All sera were collected before start of the pollen season and tested using a line blot assay (Euroimmun AG, Lübeck, Germany) for IgE to birch and timothy pollen. Both extracts, single allergens, and cross-reacting carbohydrate determinants (CCD) were analysed. Only donors with specific IgE to birch and/or timothy grass were further evaluated. Specific IgE to birch pollen extract was found in 52 sera, and sensitization to timothy grass in 40 sera. Specific IgE to Bet v 1 was predominant in contrast to Bet v 4 which was absent. However, sensitization to the minor allergens Bet v 2 and 6 was always found together with high levels of IgE to Bet v 1. Subjects sensitized to the profilin Bet v 2 from birch were also sensitized to Phl p 12 from timothy grass. In conclusion, there was predominantly Bet v 1 sensitization in this cohort and low sensitization to minor allergens and cross-reactive allergens (Bet v 2, Bet v 4, Phl p 7 and Phl p 12).
    MeSH term(s) Humans ; Betula ; Phleum ; Blood Donors ; Immunoglobulin E ; Hypersensitivity/diagnosis ; Pollen ; Allergens ; Cross Reactions
    Chemical Substances Immunoglobulin E (37341-29-0) ; Allergens
    Language English
    Publishing date 2023-11-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 3150-1
    ISSN 1502-7686 ; 0036-5513
    ISSN (online) 1502-7686
    ISSN 0036-5513
    DOI 10.1080/00365513.2023.2255972
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  2. Article ; Online: Effects of storage on quality and function of acid-treated platelets with reduced HLA Class I surface expression.

    Mirlashari, Mohammad Reza / Vetlesen, Annette / Nissen-Meyer, Lise Sofie H / Hetland, Geir

    Transfusion medicine (Oxford, England)

    2023  Volume 33, Issue 4, Page(s) 329–336

    Abstract: Background: Refractory patients need to be provided with HLA-matched platelets (PLTs), which require time-consuming cross-matching. Treatment of PLTs with citric acid leads to denaturation of the HLA Class I complexes without significant damage to the ... ...

    Abstract Background: Refractory patients need to be provided with HLA-matched platelets (PLTs), which require time-consuming cross-matching. Treatment of PLTs with citric acid leads to denaturation of the HLA Class I complexes without significant damage to the PLTs. HLA Class I depleted PLTs could alternatively be used to HLA-matched PLTs for transfusion. These PLTs have verified normal function up to 4-6 h after acid treatment.
    Materials and methods: Buffy coat (BC) PLT concentrates were depleted of HLA Class I complexes by incubation in citric acid. The days after acid-treatment, surface expression of HLA Class I complexes, CD62P and CD63 were determined by flow cytometry, in addition to viability and mitochondrial membrane potential (MMP). Thromboelastography (TEG) tested PLT functionality.
    Results: Expression of HLA Class I complexes was reduced by 70%-75% in acid-treated PLTs compared to untreated PLTs from day 1 through day 7. Controls and acid-treated PLTs showed insignificant loss of MMP stored for 4 days. Analysis of the residual PLT activation and viability showed no significant differences for 4 days of storage. However, the residual PLT activation potential and viability were significantly decreased in acid-treated PLTs and control PLTs after 7 days of storage. Acid treatment caused a significant decrease in the TEG variable, reaction time (R time), for acid-treated PLTs as compared to control PLTs from days 1 through day 3.
    Conclusion: Our data suggest that extended storage of acid-treated PLTs is possible and will improve flexibility when planning for transfusion of patients with alloimmune PLT refractoriness caused by anti-HLA-antibodies.
    MeSH term(s) Humans ; Blood Platelets ; Platelet Transfusion ; Flow Cytometry ; Blood Grouping and Crossmatching ; Citric Acid/metabolism ; Blood Preservation
    Chemical Substances Citric Acid (2968PHW8QP)
    Language English
    Publishing date 2023-06-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 1067989-3
    ISSN 1365-3148 ; 0958-7578
    ISSN (online) 1365-3148
    ISSN 0958-7578
    DOI 10.1111/tme.12983
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article: Safety of hypoxic red blood cell administration in patients with transfusion-dependent hematological malignancies: An interim analysis.

    Reikvam, Håkon / Hetland, Geir / Ezligini, Farshid / Dorsch, Kim / Omert, Laurel / Dunham, Andrew / Almeland, Stian K

    Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis

    2023  Volume 62, Issue 5, Page(s) 103755

    Abstract: Anemia is a common symptom of hematological malignancies and red blood cell (RBC) transfusion is the primary supportive treatment, with many patients becoming transfusion dependent. Hemanext Inc. (Lexington, MA, United States) has developed a CE mark ... ...

    Abstract Anemia is a common symptom of hematological malignancies and red blood cell (RBC) transfusion is the primary supportive treatment, with many patients becoming transfusion dependent. Hemanext Inc. (Lexington, MA, United States) has developed a CE mark certified device to process and store RBCs hypoxically - citrate-phosphatedextrose (CPD)/phosphate-adenine-glucose-guanosine-saline-mannitol (PAGGSM) RBCs, leukocytes-reduced (LR), O
    MeSH term(s) Humans ; Male ; Aged ; Female ; Carbon Dioxide ; Erythrocytes/chemistry ; Anemia ; Hematologic Neoplasms/therapy ; Hematologic Neoplasms/complications ; Hypoxia/therapy ; Hemoglobins/analysis
    Chemical Substances Carbon Dioxide (142M471B3J) ; citrate phosphate dextrose (51404-37-6) ; Hemoglobins
    Language English
    Publishing date 2023-06-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2046795-3
    ISSN 1878-1683 ; 1473-0502
    ISSN (online) 1878-1683
    ISSN 1473-0502
    DOI 10.1016/j.transci.2023.103755
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  4. Article ; Online: [No title information]

    Nissen-Meyer, Lise Sofie H / Ezligini, Farshid / Bergerud, Unni E / Holtan, Anders / Akkök, Çiğdem A / Danilova, Elena / Hetland, Geir

    Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke

    2022  Volume 142, Issue 11

    Title translation Blod og beredskap.
    MeSH term(s) Disaster Planning ; Humans
    Language Norwegian
    Publishing date 2022-08-02
    Publishing country Norway
    Document type Journal Article ; Comment
    ZDB-ID 603504-8
    ISSN 0807-7096 ; 0029-2001
    ISSN (online) 0807-7096
    ISSN 0029-2001
    DOI 10.4045/tidsskr.22.0494
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: DNase analysed by a novel competitive assay in patients with complications after ChAdOx1 nCoV-19 vaccination and in normal unvaccinated blood donors.

    Fagerhol, Magne Kristoffer / Schultz, Nina Haagenrud / Mirlashari, Mohammad Reza / Wiedmann, Markus Karl Hermann / Nissen-Meyer, Lise Sofie Haug / Søraas, Arne Vasli Lund / Hetland, Geir

    Scandinavian journal of immunology

    2023  Volume 98, Issue 1, Page(s) e13274

    Abstract: Increased levels of neutrophil extracellular traps (NETs) have been detected in individuals with vaccine complications after the ChAdOx1 nCov vaccine with a correlation between the severity of vaccine side effects and the level of NETosis. DNases may ... ...

    Abstract Increased levels of neutrophil extracellular traps (NETs) have been detected in individuals with vaccine complications after the ChAdOx1 nCov vaccine with a correlation between the severity of vaccine side effects and the level of NETosis. DNases may disrupt NETs by degrading their content of DNA, and a balance has been reported between NETs and DNases. Because of this and since the inflammatory marker NETs may be used as a confirmatory test in diagnosing VITT, it is of interest to monitor levels of DNase in patients with increased NETs levels. The current novel rapid DNase ELISA was tested in blood samples of patients with known increased levels of NETs with or without VITT after ChAdOx1 nCoV-19 vaccination. DNase levels in VITT patients were significantly increased compared with normal unvaccinated blood donors and compared with patients with post-vaccination symptoms but not VITT. However, since EDTA was found to inhibit DNase, serum and not EDTA-plasma samples should be applied for DNase testing. The novel DNase assay may serve as a supplementary test to the NETs test when analysing samples from patients with suspected increased NETs levels.
    MeSH term(s) Humans ; Deoxyribonucleases ; ChAdOx1 nCoV-19 ; Blood Donors ; Vaccination/adverse effects ; Drug-Related Side Effects and Adverse Reactions
    Chemical Substances Deoxyribonucleases (EC 3.1.-) ; ChAdOx1 nCoV-19 (B5S3K2V0G8)
    Language English
    Publishing date 2023-05-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 120476-2
    ISSN 1365-3083 ; 0300-9475
    ISSN (online) 1365-3083
    ISSN 0300-9475
    DOI 10.1111/sji.13274
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article ; Online: Can medicinal mushrooms have prophylactic or therapeutic effect against COVID-19 and its pneumonic superinfection and complicating inflammation?

    Hetland, Geir / Johnson, Egil / Bernardshaw, Soosaipillai V / Grinde, Bjørn

    Scandinavian journal of immunology

    2020  Volume 93, Issue 1, Page(s) e12937

    Abstract: Medicinal mushrooms have documented effects against different diseases, including infections and inflammatory disorders. The related Basidiomycota Agaricus blazei Murill (AbM), Hericium erinaceus (HE), and Grifola frondosa (GF) have been shown to exert ... ...

    Abstract Medicinal mushrooms have documented effects against different diseases, including infections and inflammatory disorders. The related Basidiomycota Agaricus blazei Murill (AbM), Hericium erinaceus (HE), and Grifola frondosa (GF) have been shown to exert antimicrobial activity against viral agents, Gram-positive and Gram-negative bacteria, and parasites in vitro and in vivo. Since the mechanism is immunomodulatory and not antibiotical, the mushrooms should be active against multi-drug resistant microbes as well. Moreover, since these Basidiomycota also have anti-inflammatory properties, they may be suited for treatment of the severe lung inflammation that often follows COVID-19 infection. An AbM-based mushroom extract (Andosan™), also containing HE and GF, has been shown to significantly reduce bacteraemia and increase survival in mice with pneumococcal sepsis, and to improve symptoms and quality of life in IBD patients via an anti-inflammatory effect. Hence, such mushroom extracts could have prophylactic or therapeutic effect against the pneumonic superinfection and severe lung inflammation that often complicates COVID-19 infection. Here, we review antimicrobial and anti-inflammatory properties of AbM, HE and GF mushrooms, which could be used for the battle against COVID-19.
    MeSH term(s) Agaricales ; Anti-Infective Agents/therapeutic use ; Anti-Inflammatory Agents/therapeutic use ; COVID-19/complications ; COVID-19/prevention & control ; COVID-19/therapy ; Humans ; Immunologic Factors/pharmacology ; SARS-CoV-2
    Chemical Substances Anti-Infective Agents ; Anti-Inflammatory Agents ; Immunologic Factors
    Keywords covid19
    Language English
    Publishing date 2020-07-29
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 120476-2
    ISSN 1365-3083 ; 0300-9475
    ISSN (online) 1365-3083
    ISSN 0300-9475
    DOI 10.1111/sji.12937
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: Can medicinal mushrooms have prophylactic or therapeutic effect against COVID‐19 and its pneumonic superinfection and complicating inflammation?

    Hetland, Geir / Johnson, Egil / Bernardshaw, Soosaipillai V. / Grinde, Bjørn

    Scandinavian Journal of Immunology ; ISSN 0300-9475 1365-3083

    2020  

    Keywords Immunology ; General Medicine ; covid19
    Language English
    Publisher Wiley
    Publishing country us
    Document type Article ; Online
    DOI 10.1111/sji.12937
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  8. Article ; Online: IgE-sensitization to food and inhalant allergens in IBD patients compared with normal blood donors at Oslo University Hospital, Norway.

    Johnson, Egil / Therkelsen, Stig Palm / Nentwich, Ivo / Nissen-Meyer, Lise Sofie Haug / Hetland, Geir

    Scandinavian journal of gastroenterology

    2019  Volume 54, Issue 9, Page(s) 1107–1110

    Abstract: Objectives: ...

    Abstract Objectives:
    MeSH term(s) Adult ; Aged ; Allergens/immunology ; Blood Donors ; Case-Control Studies ; Colitis, Ulcerative/blood ; Colitis, Ulcerative/immunology ; Crohn Disease/blood ; Crohn Disease/immunology ; Female ; Food Hypersensitivity/immunology ; Hospitals, University ; Humans ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Male ; Middle Aged ; Norway ; Young Adult
    Chemical Substances Allergens ; Immunoglobulin E (37341-29-0)
    Language English
    Publishing date 2019-09-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 82042-8
    ISSN 1502-7708 ; 0036-5521
    ISSN (online) 1502-7708
    ISSN 0036-5521
    DOI 10.1080/00365521.2019.1663445
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article ; Online: HLA class I depletion by citric acid, and irradiation of apheresis platelets for transfusion of refractory patients.

    Mirlashari, Mohammad Reza / Vetlesen, Annette / Nissen-Meyer, Lise Sofie H / Naper, Christian / Tjønnfjord, Geir Erland / Njerve, Ida Unhammer / Ezligini, Farshid / Landmark, Brynjar Fowels / Meinke, Stephan / Sandgren, Per / Höglund, Petter / Hetland, Geir

    Transfusion

    2021  Volume 61, Issue 4, Page(s) 1222–1234

    Abstract: Background: Patients can form antibodies to foreign human leukocyte antigen (HLA) Class I antigens after exposure to allogeneic cells. These anti-HLA class I antibodies can bind transfused platelets (PLTs) and mediate their destruction, thus leading to ... ...

    Abstract Background: Patients can form antibodies to foreign human leukocyte antigen (HLA) Class I antigens after exposure to allogeneic cells. These anti-HLA class I antibodies can bind transfused platelets (PLTs) and mediate their destruction, thus leading to PLT refractoriness. Patients with PLT refractoriness need HLA-matched PLTs, which require expensive HLA typing of donors, antibody analyses of patient sera and/or crossmatching. An alternative approach is to reduce PLT HLA Class I expression using a brief incubation in citric acid on ice at low pH.
    Methods and materials: Apheresis PLT concentrates were depleted of HLA Class I complexes by 5 minutes incubation in ice-cold citric acid, at pH 3.0. Surface expression of HLA Class I complexes, CD62P, CD63, phosphatidylserine, and complement factor C3c was analyzed by flow cytometry. PLT functionality was tested by thromboelastography (TEG).
    Results: Acid treatment reduced the expression of HLA Class I complexes by 71% and potential for C3c binding by 11.5-fold compared to untreated PLTs. Acid-treated PLTs were significantly more activated than untreated PLTs, but irrespective of this increase in steady-state activation, CD62P and CD63 were strongly upregulated on both acid-treated and untreated PLTs after stimulation with thrombin receptor agonist peptide. Acid treatment did not induce apoptosis over time. X-ray irradiation did not significantly influence the expression of HLA Class I complexes, CD62P, CD63, and TEG variables on acid treated PLTs.
    Conclusion: The relatively simple acid stripping method can be used with irradiated apheresis PLTs and may prevent transfusion-associated HLA sensitization and overcome PLT refractoriness.
    MeSH term(s) Antibodies/immunology ; Blood Grouping and Crossmatching/methods ; Blood Platelets/radiation effects ; Citric Acid/adverse effects ; Female ; Histocompatibility Antigens Class I/drug effects ; Histocompatibility Antigens Class I/immunology ; Histocompatibility Antigens Class I/metabolism ; Histocompatibility Antigens Class I/radiation effects ; Histocompatibility Testing/economics ; Histocompatibility Testing/methods ; Humans ; P-Selectin/metabolism ; Platelet Transfusion/adverse effects ; Platelet Transfusion/methods ; Plateletpheresis/methods ; Severe Combined Immunodeficiency/chemically induced ; Tetraspanin 30/metabolism ; Thrombelastography/methods ; Thrombocytopenia/therapy ; Up-Regulation/genetics
    Chemical Substances Antibodies ; Histocompatibility Antigens Class I ; P-Selectin ; Tetraspanin 30 ; Citric Acid (2968PHW8QP)
    Language English
    Publishing date 2021-02-13
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.16282
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article: Antitumor, Anti-inflammatory and Antiallergic Effects of Agaricus blazei Mushroom Extract and the Related Medicinal Basidiomycetes Mushrooms, Hericium erinaceus and Grifola frondosa: A Review of Preclinical and Clinical Studies

    Hetland, Geir / Tangen, Jon-Magnus / Mahmood, Faiza / Mirlashari, Mohammad Reza / Nissen-Meyer, Lise Sofie Haug / Nentwich, Ivo / Therkelsen, Stig Palm / Tjønnfjord, Geir Erland / Johnson, Egil

    Nutrients. 2020 May 08, v. 12, no. 5

    2020  

    Abstract: Since the 1980s, medicinal effects have been documented in scientific studies with the related Basidiomycota mushrooms Agaricus blazei Murill (AbM), Hericium erinaceus (HE) and Grifola frondosa (GF) from Brazilian and Eastern traditional medicine. ... ...

    Abstract Since the 1980s, medicinal effects have been documented in scientific studies with the related Basidiomycota mushrooms Agaricus blazei Murill (AbM), Hericium erinaceus (HE) and Grifola frondosa (GF) from Brazilian and Eastern traditional medicine. Special focus has been on their antitumor effects, but the mushrooms’ anti-inflammatory and antiallergic properties have also been investigated. The antitumor mechanisms were either direct tumor attack, e.g., apoptosis and metastatic suppression, or indirect defense, e.g., inhibited tumor neovascularization and T helper cell (Th) 1 immune response. The anti-inflammatory mechanisms were a reduction in proinflammatory cytokines, oxidative stress and changed gut microbiota, and the antiallergic mechanism was amelioration of a skewed Th1/Th2 balance. Since a predominant Th2 milieu is also found in cancer, which quite often is caused by a local chronic inflammation, the three conditions—tumor, inflammation and allergy—seem to be linked. Further mechanisms for HE were increased nerve and beneficial gut microbiota growth, and oxidative stress regulation. The medicinal mushrooms AbM, HE and GF appear to be safe, and can, in fact, increase longevity in animal models, possibly due to reduced tumorigenesis and oxidation. This article reviews preclinical and clinical findings with these mushrooms and the mechanisms behind them.
    Keywords Agaricus subrufescens ; CD4-positive T-lymphocytes ; Erinaceus ; Grifola frondosa ; Hericium ; angiogenesis ; animal models ; anti-allergic agents ; antineoplastic activity ; apoptosis ; carcinogenesis ; clinical trials ; cytokines ; immune response ; inflammation ; intestinal microorganisms ; longevity ; medicinal fungi ; metastasis ; mushrooms ; neoplasms ; nerve tissue ; nutrients ; oxidation ; oxidative stress ; traditional medicine
    Language English
    Dates of publication 2020-0508
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2518386-2
    ISSN 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu12051339
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

To top