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  1. Article: Two Methods of AuNPs Synthesis Induce Differential Vascular Effects. The Role of the Endothelial Glycocalyx.

    Maldonado-Ortega, Daniel Alberto / Martínez-Castañón, Gabriel / Palestino, Gabriela / Navarro-Tovar, Gabriela / Gonzalez, Carmen

    Frontiers in medicine

    2022  Volume 9, Page(s) 889952

    Abstract: AuNPs are synthesized through several methods to tune their physicochemical properties. Although AuNPs are considered biocompatible, a change in morphology or properties can modify their biological impact. In this work, AuNPs (~12 to 16 nm) capping with ... ...

    Abstract AuNPs are synthesized through several methods to tune their physicochemical properties. Although AuNPs are considered biocompatible, a change in morphology or properties can modify their biological impact. In this work, AuNPs (~12 to 16 nm) capping with either sodium citrate (CA) or gallic acid (GA) were evaluated in a rat aorta
    Language English
    Publishing date 2022-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2022.889952
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  2. Article ; Online: Two Methods of AuNPs Synthesis Induce Differential Vascular Effects. The Role of the Endothelial Glycocalyx

    Daniel Alberto Maldonado-Ortega / Gabriel Martínez-Castañón / Gabriela Palestino / Gabriela Navarro-Tovar / Carmen Gonzalez

    Frontiers in Medicine, Vol

    2022  Volume 9

    Abstract: AuNPs are synthesized through several methods to tune their physicochemical properties. Although AuNPs are considered biocompatible, a change in morphology or properties can modify their biological impact. In this work, AuNPs (~12 to 16 nm) capping with ... ...

    Abstract AuNPs are synthesized through several methods to tune their physicochemical properties. Although AuNPs are considered biocompatible, a change in morphology or properties can modify their biological impact. In this work, AuNPs (~12 to 16 nm) capping with either sodium citrate (CA) or gallic acid (GA) were evaluated in a rat aorta ex vivo model, which endothelial inner layer surface is formed by glycocalyx (hyaluronic acid, HA, as the main component), promoting vascular processes, most of them dependent on nitric oxide (NO) production. Results showed that contractile effects were more evident with AuNPsCA, while dilator effects predominated with AuNPsGA. Furthermore, treatments with AuNPsCA and AuNPsGA in the presence or absence of glycocalyx changed the NO levels, differently. This work contributes to understanding the biological effects of AuNPs with different capping agents, as well as the key role that of HA in the vascular effects induced by AuNPs in potential biomedical applications.
    Keywords gold nanoparticles ; aorta ; vascular tone ; endothelium ; glycocalyx ; nitric oxide ; Medicine (General) ; R5-920
    Subject code 500
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Use of Trehalose as an Additive to Bacteriophage Vb_Pd_PDCC-1: Long-Term Preservation Analysis and Its Biocontrol Against Vibrio diabolicus Infection.

    Moreno-Figueroa, Luis Daniel / Quiroz-Guzmán, Eduardo / Tovar-Ramírez, Dariel / Palestino, Gabriela / Cisneros-Covarrubias, Cándida A / Hernández-Adame, Luis

    Current microbiology

    2023  Volume 80, Issue 12, Page(s) 372

    Abstract: Phage therapy is a promising alternative to control bacterial diseases and the increasing problem of antibiotic resistance. In this sense, this research evaluates the viability of lyophilized vibrio phage vB_Pd_PDCC-1 using trehalose as a preservative ... ...

    Abstract Phage therapy is a promising alternative to control bacterial diseases and the increasing problem of antibiotic resistance. In this sense, this research evaluates the viability of lyophilized vibrio phage vB_Pd_PDCC-1 using trehalose as a preservative excipient at different concentrations (4, 2, 1, and 0.5% w/v) and its potential for phage therapy application against a pathogenic bacteria Vibrio diabolicus in brine shrimp nauplii (Artemia franciscana). The lyophilized phages were stored at 4 and 23 °C and rehydrated using biological sterile saline solution to test their viability at days 1, 15, and 60 post-lyophilization. The results showed that trehalose is beneficial in maintaining the viability of post-lyophilization phages (without titer losses) at 4 °C and even at room temperature (23 °C). When lyophilized phages with 4% w/v trehalose concentration were stored at 23 °C, they had not titer losses among the trials; viability and titer concentration were maintained up to 60 days at log 7. The use of lyophilized phage PDCC-1 increased brine shrimp survival and reduced Vibrio concentrations. The present study has identified trehalose as a promising lyophilization excipient to effectively preserve lyophilized bacteriophages for biotechnological applications and long-term storage.
    MeSH term(s) Bacteriophages ; Trehalose/pharmacology ; Excipients ; Vibrio ; Myoviridae
    Chemical Substances Trehalose (B8WCK70T7I) ; Excipients
    Language English
    Publishing date 2023-10-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-023-03487-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Core-shell chitosan/Porphyridium-exopolysaccharide microgels: Synthesis, properties, and biological evaluation.

    Ruiz-Dávila, Claudia Elizabeth / Solís-Andrade, Karla Ivón / Olvera-Sosa, Miguel / Palestino, Gabriela / Rosales-Mendoza, Sergio

    International journal of biological macromolecules

    2023  Volume 246, Page(s) 125655

    Abstract: Advanced materials used in the biomedicine field comprises a diverse group of organic molecules, including polymers, polysaccharides, and proteins. A significant trend in this area is the design of new micro/nano gels whose small size, physical stability, ...

    Abstract Advanced materials used in the biomedicine field comprises a diverse group of organic molecules, including polymers, polysaccharides, and proteins. A significant trend in this area is the design of new micro/nano gels whose small size, physical stability, biocompatibility, and bioactivity could lead to new applications. Herein a new synthesis route is described to obtain core-shell microgels based on chitosan and Porphyridium exopolysaccharides (EPS) crosslinked with sodium tripolyphosphate (TPP). First, the synthesis of EPS-chitosan gels through ionic interactions was explored, leading to the formation of unstable gels. Alternatively, the use of TTP as crosslinker agent led to stable core-shell structures. The influence of reaction temperature, sonication time, and exopolysaccharide concentration, pH and TPP concentration were determined as a function of particle size and polydispersity index (PDI). The obtained EPS-chitosan gels were characterized by TEM, TGA, and FTIR; followed by the assessment of protein load capacity, stability upon freezing, cytotoxicity, and mucoadhesivity. Experimentation revealed that the core-shell particles size ranges 100-300 nm, have a 52 % loading capacity for BSA and a < 90 % mucoadhesivity, and no toxic effects in mammalian cell cultures. The potential application of the obtained microgels in the biomedical field is discussed.
    MeSH term(s) Animals ; Chitosan/chemistry ; Microgels ; Porphyridium ; Gels/chemistry ; Ions ; Particle Size ; Mammals
    Chemical Substances Chitosan (9012-76-4) ; Microgels ; Gels ; Ions
    Language English
    Publishing date 2023-07-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2023.125655
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  5. Article: Synthesis and Characterization of Innovative Microgels Based on Polyacrylic Acid and Microalgae Cell Wall and Their Potential as Antigen Delivery Vehicles.

    García-Silva, Ileana / Olvera-Sosa, Miguel / Ortega-Berlanga, Benita / Ruíz-Rodríguez, Víctor / Palestino, Gabriela / Rosales-Mendoza, Sergio

    Pharmaceutics

    2022  Volume 15, Issue 1

    Abstract: In this study, hybrid polyacrylic acid ... ...

    Abstract In this study, hybrid polyacrylic acid and
    Language English
    Publishing date 2022-12-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics15010133
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  6. Article ; Online: Core-shell chitosan/Porphyridium-exopolysaccharide microgels: Synthesis, properties, and biological evaluation

    Ruiz-Dávila, Claudia Elizabeth / Solís Andrade, Karla Ivon / Olvera-Sosa, Miguel / Palestino, Gabriela / Rosales Mendoza, Sergio

    International Journal of Biological Macromolecules. 2023 Aug., v. 246 p.125655-

    2023  

    Abstract: Advanced materials used in the biomedicine field comprises a diverse group of organic molecules, including polymers, polysaccharides, and proteins. A significant trend in this area is the design of new micro/nano gels whose small size, physical stability, ...

    Abstract Advanced materials used in the biomedicine field comprises a diverse group of organic molecules, including polymers, polysaccharides, and proteins. A significant trend in this area is the design of new micro/nano gels whose small size, physical stability, biocompatibility, and bioactivity could lead to new applications. Herein a new synthesis route is described to obtain core-shell microgels based on chitosan and Porphyridium exopolysaccharides (EPS) crosslinked with sodium tripolyphosphate (TPP). First, the synthesis of EPS-chitosan gels through ionic interactions was explored, leading to the formation of unstable gels. Alternatively, the use of TTP as crosslinker agent led to stable core-shell structures. The influence of reaction temperature, sonication time, and exopolysaccharide concentration, pH and TPP concentration were determined as a function of particle size and polydispersity index (PDI). The obtained EPS-chitosan gels were characterized by TEM, TGA, and FTIR; followed by the assessment of protein load capacity, stability upon freezing, cytotoxicity, and mucoadhesivity. Experimentation revealed that the core-shell particles size ranges 100–300 nm, have a 52 % loading capacity for BSA and a < 90 % mucoadhesivity, and no toxic effects in mammalian cell cultures. The potential application of the obtained microgels in the biomedical field is discussed.
    Keywords Porphyridium ; biocompatibility ; biological assessment ; chitosan ; crosslinking ; cytotoxicity ; exopolysaccharides ; mammals ; medicine ; microgels ; pH ; particle size ; sodium tripolyphosphate ; sonication ; temperature ; Hydrogel ; Core-shell
    Language English
    Dates of publication 2023-08
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2023.125655
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: An overview on the role of silica-based materials in vaccine development.

    Navarro-Tovar, Gabriela / Palestino, Gabriela / Rosales-Mendoza, Sergio

    Expert review of vaccines

    2016  Volume 15, Issue 11, Page(s) 1449–1462

    Abstract: Introduction: Although vaccination has prevented millions of deaths, the development of highly immunogenic subunit vaccines is still required. Since the number of adjuvants approved for human use is limited, the new paths for the development of delivery ...

    Abstract Introduction: Although vaccination has prevented millions of deaths, the development of highly immunogenic subunit vaccines is still required. Since the number of adjuvants approved for human use is limited, the new paths for the development of delivery vehicles offered by nanotechnology are of key relevance. Areas covered: Herein, the potential of silica nanoparticles (SP) as both adjuvants and vaccine delivery vehicles is discussed based on the analysis of the current biomedical literature. Expert commentary: SP are reported not only as biodegradable and biocompatible material but also as easy to modify and with a low production cost. Additionally, several reports suggest that SP enhance the immune response. Therefore, SP are a promising delivery vehicle and/or adjuvant in vaccines. However, knowledge on the industrial production and specific aspects of immunity are still required.
    MeSH term(s) Adjuvants, Immunologic/administration & dosage ; Animals ; Drug Carriers/administration & dosage ; Humans ; Models, Animal ; Nanoparticles/administration & dosage ; Silicon Dioxide/administration & dosage ; Vaccines/administration & dosage ; Vaccines/immunology
    Chemical Substances Adjuvants, Immunologic ; Drug Carriers ; Vaccines ; Silicon Dioxide (7631-86-9)
    Language English
    Publishing date 2016-11
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2181284-6
    ISSN 1744-8395 ; 1476-0584
    ISSN (online) 1744-8395
    ISSN 1476-0584
    DOI 10.1080/14760584.2016.1188009
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    Zs.A 6077: Show issues Location:
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  8. Article ; Online: Chitosan sponges loaded with metformin and microalgae as dressing for wound healing: A study in diabetic bio-models.

    Vazquez-Ayala, Laura / Del Ángel-Olarte, César / Escobar-García, Diana María / Rosales-Mendoza, Sergio / Solis-Andrade, Ivon / Pozos-Guillén, Amaury / Palestino, Gabriela

    International journal of biological macromolecules

    2023  Volume 254, Issue Pt 1, Page(s) 127691

    Abstract: Among the conditions caused by diabetes, the diabetic foot is a significant public health problem due to its delayed healing process. That makes it essential to design, manufacture, and apply auxiliary dressings during healing. In this work, chitosan ... ...

    Abstract Among the conditions caused by diabetes, the diabetic foot is a significant public health problem due to its delayed healing process. That makes it essential to design, manufacture, and apply auxiliary dressings during healing. In this work, chitosan sponges were developed and evaluated as wound dressings. Metformin, fucoidan, and exopolysaccharide from Porphyridium purpureum algae were loaded into the sponges and studied as healing promoters. The composite sponges were physicochemically, morphologically, and thermally characterized, allowing us to determine the chemical mechanisms involved in the sponge formation. The mechanical analysis demonstrated that sponge composites have shape memory and good mechanical performance under compression stress, showing a compressive strength above 30 kPa. These results correlated with the materials' porosity, influencing the swelling capacity that reached a maximum of 70 %. The morphology of materials was observed by SEM, resulting in folded films with surface porosity. The results of the biocompatibility tests confirmed that the materials are not cytotoxic or hemolytic and have good antibacterial activity. In vivo wound healing evaluation showed that metformin-loaded chitosan sponges regenerated skin tissue after 21 days of treatment, highlighting the rate of healing provided when exopolysaccharide was added to promote tissue regeneration, which can be corroborated by histological analysis. These results make chitosan sponge compounds promising dressings for diabetic foot wound treatment.
    MeSH term(s) Humans ; Chitosan/chemistry ; Microalgae ; Diabetic Foot ; Wound Healing ; Anti-Bacterial Agents/pharmacology ; Bandages ; Diabetes Mellitus
    Chemical Substances Chitosan (9012-76-4) ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-10-28
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2023.127691
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  9. Article ; Online: Can nanotechnology help in the fight against COVID-19?

    Palestino, Gabriela / García-Silva, Ileana / González-Ortega, Omar / Rosales-Mendoza, Sergio

    Expert review of anti-infective therapy

    2020  Volume 18, Issue 9, Page(s) 849–864

    Abstract: Introduction: The current COVID-19 pandemic caused by the SARS-CoV-2 virus demands the development of strategies not only to detect or inactivate the virus, but to treat it (therapeutically and prophylactically). COVID-19 is not only a critical threat ... ...

    Abstract Introduction: The current COVID-19 pandemic caused by the SARS-CoV-2 virus demands the development of strategies not only to detect or inactivate the virus, but to treat it (therapeutically and prophylactically). COVID-19 is not only a critical threat for the population with risk factors, but also generates a dramatic economic impact in terms of morbidity and the overall interruption of economic activities.
    Areas covered: Advanced materials are the basis of several technologies that could diminish the impact of COVID-19: biosensors might allow early virus detection, nanosized vaccines are powerful agents that could prevent viral infections, and nanosystems with antiviral activity could bind the virus for inactivation or destruction upon application of an external stimulus. Herein all these methods are discussed under the light of cutting-edge technologies and the previously reported prototypes targeting enveloped viruses similar to SARS-CoV-2. This analysis was derived from an extensive scientific literature search (including pubmed) performed on April 2020.
    Expert opinion: Perspectives on how biosensors, vaccines, and antiviral nanosystems can be implemented to fight COVID-19 are envisioned; identifying the approaches that can be implemented in the short term and those that deserve long term research to cope with respiratory viruses-related pandemics in the future.
    MeSH term(s) Betacoronavirus/isolation & purification ; Betacoronavirus/physiology ; Biosensing Techniques/methods ; COVID-19 ; COVID-19 Vaccines ; Coronavirus Infections/diagnosis ; Coronavirus Infections/prevention & control ; Coronavirus Infections/therapy ; Humans ; Nanostructures/therapeutic use ; Nanotechnology/methods ; Pandemics/prevention & control ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/prevention & control ; Pneumonia, Viral/therapy ; SARS-CoV-2 ; Viral Vaccines/pharmacology
    Chemical Substances COVID-19 Vaccines ; Viral Vaccines
    Keywords covid19
    Language English
    Publishing date 2020-06-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2181279-2
    ISSN 1744-8336 ; 1478-7210
    ISSN (online) 1744-8336
    ISSN 1478-7210
    DOI 10.1080/14787210.2020.1776115
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    Zs.A 6106: Show issues Location:
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  10. Article ; Online: Synthesis of Bamboo-like Multiwall Carbon Nanotube-Poly(Acrylic Acid-co-Itaconic Acid)/NaOH Composite Hydrogel and its Potential Application for Electrochemical Detection of Cadmium(II).

    Chazaro-Ruiz, Luis F / Olvera-Sosa, Miguel / Vidal, Gabriela / Rangel-Mendez, J Rene / Palestino, Gabriela / Perez, Fatima / Zhang, Wei

    Biosensors

    2020  Volume 10, Issue 10

    Abstract: A poly(acrylic acid-co-itaconic acid) (PAA-co-IA)/NaOH hydrogel containing bamboo-type multiwall carbon nanotubes (B-MWCNTs) doped with nitrogen (PAA-co-IA/NaOH/B-MWCNTs) was synthesized and characterized by SEM, absorption of water, point of zero ... ...

    Abstract A poly(acrylic acid-co-itaconic acid) (PAA-co-IA)/NaOH hydrogel containing bamboo-type multiwall carbon nanotubes (B-MWCNTs) doped with nitrogen (PAA-co-IA/NaOH/B-MWCNTs) was synthesized and characterized by SEM, absorption of water, point of zero charges, infrared spectroscopy, thermogravimetric analysis, and differential scanning calorimetry. The possible use of the PAA-co-IA/NaOH/B-MWCNT hydrogel as an electrode modifier and pre-concentrator agent for Cd(II) sensing purposes was then evaluated using carbon paste electrodes via differential pulse voltammetry. The presence of the B-MWCNTs in the hydrogel matrix decreased its degree of swelling, stabilized the structure of the swollen gel, and favored the detection of 3 ppb Cd(II), which is comparable to the World Health Organization's allowable maximum value in drinking water. A calibration curve was obtained in the concentration range of 2.67 × 10
    MeSH term(s) Acrylates ; Cadmium/analysis ; Electrochemical Techniques ; Electrodes ; Graphite ; Hydrogels/chemistry ; Ions ; Limit of Detection ; Mercury ; Nanotubes, Carbon/chemistry ; Sodium Hydroxide/chemistry ; Succinates ; Water
    Chemical Substances Acrylates ; Hydrogels ; Ions ; Nanotubes, Carbon ; Succinates ; Cadmium (00BH33GNGH) ; Water (059QF0KO0R) ; Sodium Hydroxide (55X04QC32I) ; Graphite (7782-42-5) ; Mercury (FXS1BY2PGL) ; acrylic acid (J94PBK7X8S) ; itaconic acid (Q4516562YH)
    Language English
    Publishing date 2020-10-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios10100147
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