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  1. Article ; Online: Missense mutation of a class B heat shock factor is responsible for the tomato bushy root-2 phenotype.

    Kevei, Zoltan / Ferreira, Silva Demetryus Silva / Casenave, Cristina Maria Perez / Kurowski, Tomasz / Mohareb, Fady / Rickett, Daniel / Stain, Chris / Thompson, Andrew J

    Molecular horticulture

    2022  Volume 2, Issue 1, Page(s) 4

    Abstract: ... S75C) substitution in the DNA binding domain (DBD) of a heat shock factor class B (HsfB) encoded ...

    Abstract The bushy root-2 (brt-2) tomato mutant has twisting roots, and slower plant development. Here we used whole genome resequencing and genetic mapping to show that brt-2 is caused by a serine to cysteine (S75C) substitution in the DNA binding domain (DBD) of a heat shock factor class B (HsfB) encoded by SolycHsfB4a. This gene is orthologous to the Arabidopsis SCHIZORIZA gene, also known as AtHsfB4. The brt-2 phenotype is very similar to Arabidopsis lines in which the function of AtHsfB4 is altered: a proliferation of lateral root cap and root meristematic tissues, and a tendency for lateral root cap cells to easily separate. The brt-2 S75C mutation is unusual because all other reported amino acid substitutions in the highly conserved DBD of eukaryotic heat shock factors are dominant negative mutations, but brt-2 is recessive. We further show through reciprocal grafting that brt-2 exerts its effects predominantly through the root genotype even through BRT-2 is expressed at similar levels in both root and shoot meristems. Since AtHsfB4 is induced by root knot nematodes (RKN), and loss-of-function mutants of this gene are resistant to RKNs, BRT-2 could be a target gene for RKN resistance, an important trait in tomato rootstock breeding.Gene & accession numbersSolycHsfB4a - Solyc04g078770.
    Language English
    Publishing date 2022-02-08
    Publishing country England
    Document type Journal Article
    ISSN 2730-9401
    ISSN (online) 2730-9401
    DOI 10.1186/s43897-022-00025-0
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  2. Article ; Online: Significant changes in the composition of the precursor B-cell compartment in children less than 2 years old.

    Piątosa, Barbara / Birbach, Mariusz / Siewiera, Katarzyna / Ussowicz, Marek / Kałwak, Krzysztof / Drabko, Katarzyna / Rękawek, Aneta / Tkaczyk, Katarzyna / Kurowski, Przemysław Norbert

    Cytometry. Part B, Clinical cytometry

    2013  Volume 84, Issue 3, Page(s) 179–186

    Abstract: Background: Defects in early B lymphocyte maturation in bone marrow (BM) compose a characteristic ... on the composition of the precursor B-cell compartment in BM is available. The aim of this study was to define normal ... age-related ranges of total B-cell content and distribution of precursor B-cell stages in BM ...

    Abstract Background: Defects in early B lymphocyte maturation in bone marrow (BM) compose a characteristic feature of many primary immune deficiencies associated with agammaglobulinemia. To date, only limited data on the composition of the precursor B-cell compartment in BM is available. The aim of this study was to define normal age-related ranges of total B-cell content and distribution of precursor B-cell stages in BM for the future use in clinical diagnostics.
    Methods: Four color flow cytometry was used to analyze the composition of the B-cell compartment in specimens from 59 hematologically healthy children, aged 14 days to 16 years, assigned to six age groups: neonates less than 1 month old, infants >1-12 months old, children >1-2 years old, >2-5 years old, >5-10 years old, and older than 10 years.
    Results: Analysis of the composition of the B-cell compartment revealed significant age-related variation in the distribution of individual B-cell maturation stages, most seriously affecting children during first 2 years of life, with the shift from domination of the earliest stages, to gradually increasing content of mature B-cells. Significantly higher proportions of pro-B lymphocytes were observed in neonates than in any other age group.
    Conclusion: Physiological age-related variation in the precursor B-cell compartment composition affects most seriously very young children below the age of 2 years. Proper interpretation of immunophenotyping results performed in cases of suspected early B-cell differentiation defect requires application of adequate reference data.
    MeSH term(s) Adolescent ; Age Factors ; Antigens, CD/analysis ; Antigens, CD/immunology ; Bone Marrow Cells/cytology ; Bone Marrow Cells/immunology ; Cell Differentiation ; Child ; Child, Preschool ; Female ; Flow Cytometry ; Humans ; Immunophenotyping/standards ; Infant ; Infant, Newborn ; Male ; Precursor Cells, B-Lymphoid/cytology ; Precursor Cells, B-Lymphoid/immunology
    Chemical Substances Antigens, CD
    Language English
    Publishing date 2013-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2099657-3
    ISSN 1552-4957 ; 1552-4949 ; 0196-4763
    ISSN (online) 1552-4957
    ISSN 1552-4949 ; 0196-4763
    DOI 10.1002/cyto.b.21085
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  3. Article ; Online: Lopinavir/ritonavir pharmacokinetics, efficacy, and safety in HIV and hepatitis B or C coinfected adults without symptoms of hepatic impairment.

    Khaykin, Pavel / Kotzerke, Peter / Stephan, Christoph / Nisius, Gabi / Bickel, Markus / Haberl, Annette / Stürmer, Martin / Kurowski, Michael / Brodt, Reinhard / von Hentig, Nils

    Therapeutic drug monitoring

    2014  Volume 36, Issue 2, Page(s) 192–201

    Abstract: ... antiretroviral therapy regimen, both in patients with HIV alone and coinfected with hepatitis B or C ...

    Abstract Objective: Lopinavir/ritonavir plus nucleoside reverse transcriptase inhibitors is one standard antiretroviral therapy regimen, both in patients with HIV alone and coinfected with hepatitis B or C. Our objective was to investigate whether hepatitis coinfection without clinical signs of hepatic impairment is a cofactor altering lopinavir pharmacokinetics and influencing therapy outcome.
    Methods: Steady-state 12-hour pharmacokinetic profiles of lopinavir/ritonavir were assessed in patients with (group 1, n = 20) or without (group 2, n = 36) hepatitis coinfection, taking lopinavir/ritonavir 400/100 mg twice a day plus nucleoside reverse transcriptase inhibitors, measured by means of high-performance liquid chromatography-tandem mass spectrometry. Demographic (sex, age, weight), pharmacological (formulation, comedication), clinical, and virological/immunologic parameters (HIV-RNA PCR, CD4(+) cell count) were compared between the groups and included in regression analyses for correlations with lopinavir pharmacokinetic parameters (C(min), C(max), AUC, CL, and t(1/2)) and viral load evolution over 48 weeks on therapy. Patient pairs were matched 1:2 for the parameters sex, age, weight, ethnicity, and drug formulation.
    Results: None of the hepatitis-related cofactors (aspartate aminotransferase, alanine aminotransferase, γGT, HBe Ag, HBsAg, HCV-RNA PCR, HCV-therapy) had an influence on lopinavir pharmacokinetics in this group of patients. Lopinavir C(min) (P = 0.039) and area under the curve (P = 0.038) and ritonavir C(max) (P = 0.049) were significantly enhanced in hepatitis-coinfected patients, but correlated only with drug formulation (ie, soft gel capsule or Meltrex tablet formulation, multivariate regression analysis, P = 0.001), not hepatitis coinfection.
    Conclusions: Despite moderately enhanced lopinavir/ritonavir plasma concentrations, regular therapeutic drug monitoring is not to be considered in hepatitis-coinfected patients without hepatic impairment. Antiviral efficacy is comparable between both groups, a less-pronounced CD4(+) cell increase in hepatitis-coinfected patients is in line with previously published data.
    MeSH term(s) Adult ; Case-Control Studies ; Chemistry, Pharmaceutical ; Coinfection/drug therapy ; Drug Combinations ; HIV Infections/complications ; HIV Infections/drug therapy ; HIV Protease Inhibitors/adverse effects ; HIV Protease Inhibitors/pharmacokinetics ; HIV Protease Inhibitors/therapeutic use ; Hepatitis B/complications ; Hepatitis B/drug therapy ; Hepatitis C/complications ; Hepatitis C/drug therapy ; Humans ; Liver/drug effects ; Lopinavir/adverse effects ; Lopinavir/pharmacokinetics ; Lopinavir/therapeutic use ; Male ; Middle Aged ; Ritonavir/adverse effects ; Ritonavir/pharmacokinetics ; Ritonavir/therapeutic use ; Treatment Outcome ; Viral Load
    Chemical Substances Drug Combinations ; HIV Protease Inhibitors ; Lopinavir (2494G1JF75) ; Ritonavir (O3J8G9O825)
    Language English
    Publishing date 2014-04
    Publishing country United States
    Document type Controlled Clinical Trial ; Journal Article
    ZDB-ID 424443-6
    ISSN 1536-3694 ; 0163-4356
    ISSN (online) 1536-3694
    ISSN 0163-4356
    DOI 10.1097/FTD.0b013e3182a28c6a
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  4. Article: Impact of amphotericin B on the cytochrome P450 system in HIV-infected patients.

    Brockmeyer, Norbert H / Gambichler, T / Bader, A / Kreuter, A / Kurowski, M / Sander, P / Altmeyer, P

    European journal of medical research

    2004  Volume 9, Issue 2, Page(s) 51–54

    Abstract: ... B (Am-B) during the treatment of Candida oesophagitis in HIV-infected patients.: Methods: Twelve ... enrolled into a prospective clinical trial. The patients were treated with Am-B (0.4 mg/kg body weight ... for two weeks. At baseline and after Am-B therapy the clearance of antipyrine and its metabolites were ...

    Abstract Objective: To investigate whether cytochrome P450-dependent enzymes are influenced by amphotericin B (Am-B) during the treatment of Candida oesophagitis in HIV-infected patients.
    Methods: Twelve HIV-infected, antiretroviral-naive patients (CDC/WHO stage C3) with Candida oesophagitits were enrolled into a prospective clinical trial. The patients were treated with Am-B (0.4 mg/kg body weight) for two weeks. At baseline and after Am-B therapy the clearance of antipyrine and its metabolites were investigated by high-performance liquid chromatography. In addition, the urinary excretion of 6-beta-hydroxycortisol and 17-hydroxycorticosteroids was assessed by means of a radioimmunoassay.
    Results: The following significant changes were observed after Am-B treatment (P < 0.01): increase of antipyrine half-life (12.4 h vs 16.8 h) and the area under the plasma concentration-time curve (27.9 mg min/ml vs 38.1 mg min/ml); decrease of the total body clearance (61.2 ml/min vs 43.7 ml/min); decrease of the renal clearance of antipyrine metabolites - norantipyrine (7.45 ml/min vs 5.31 ml/min), 4-hydroxyantipyrine (15.4 ml/min vs 10.3 ml/min), hydroxymethylantipyrine (4.31 ml/min vs 3.65 ml/min); decrease of urinary 6-beta-hydroxycortisol excretion (453 microg/24h vs 298 microg/24h) and the ratio of 6-beta-hydroycortisol to 17-hydroxycorticosteroids (8.8% vs 6.4%).
    Conclusions: Our data indicate that Am-B therapy has an inhibitory effect on cytochrome P450-dependent enzymes in HIV-infected patients. These results are of particular significance for HIV-infected patients who are concomitantly treated with drugs that are predominantly metabolised in the liver. A careful drug monitoring system seems advisable, especially for proteinase inhibitor experienced HIV-1-infected subjects.
    MeSH term(s) 17-Hydroxycorticosteroids/urine ; AIDS-Related Opportunistic Infections/drug therapy ; AIDS-Related Opportunistic Infections/enzymology ; AIDS-Related Opportunistic Infections/urine ; Amphotericin B/pharmacology ; Anti-Bacterial Agents/pharmacology ; Antipyrine/pharmacokinetics ; Candidiasis/complications ; Chromatography, High Pressure Liquid ; Cytochrome P-450 Enzyme System/drug effects ; Cytochrome P-450 Enzyme System/metabolism ; Esophagitis/drug therapy ; Esophagitis/etiology ; Esophagitis/microbiology ; Humans ; Hydrocortisone/analogs & derivatives ; Hydrocortisone/urine ; Immunocompromised Host
    Chemical Substances 17-Hydroxycorticosteroids ; Anti-Bacterial Agents ; 6 beta-hydroxycortisol (53-35-0) ; Amphotericin B (7XU7A7DROE) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Antipyrine (T3CHA1B51H) ; Hydrocortisone (WI4X0X7BPJ)
    Language English
    Publishing date 2004-02-27
    Publishing country England
    Document type Clinical Trial ; Journal Article
    ZDB-ID 1329381-3
    ISSN 2047-783X ; 0949-2321
    ISSN (online) 2047-783X
    ISSN 0949-2321
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  5. Article ; Online: A Novel Method for Intelligibility Assessment of Nonlinearly Processed Speech in Spaces Characterized by Long Reverberation Times.

    Kurowski, Adam / Kotus, Jozef / Odya, Piotr / Kostek, Bozena

    Sensors (Basel, Switzerland)

    2022  Volume 22, Issue 4

    Abstract: Objective assessment of speech intelligibility is a complex task that requires taking into account a number of factors such as different perception of each speech sub-bands by the human hearing sense or different physical properties of each frequency ... ...

    Abstract Objective assessment of speech intelligibility is a complex task that requires taking into account a number of factors such as different perception of each speech sub-bands by the human hearing sense or different physical properties of each frequency band of a speech signal. Currently, the state-of-the-art method used for assessing the quality of speech transmission is the speech transmission index (STI). It is a standardized way of objectively measuring the quality of, e.g., an acoustical adaptation of conference rooms or public address systems. The wide use of this measure and implementation of this method on numerous measurement devices make STI a popular choice when the speech-related quality of rooms has to be estimated. However, the STI measure has a significant drawback which excludes it from some particular use cases. For instance, if one would like to enhance speech intelligibility by employing a nonlinear digital processing algorithm, the STI method is not suitable to measure the impact of such an algorithm, as it requires that the measurement signal should not be altered in a nonlinear way. Consequently, if a nonlinear speech enhancing algorithm has to be tested, the STI-a standard way of estimating speech transmission cannot be used. In this work, we would like to propose a method based on the STI method but modified in such a way that it makes it possible to employ it for the estimation of the performance of the nonlinear speech intelligibility enhancement method. The proposed approach is based upon a broadband comparison of cumulated energy of the transmitted envelope modulation and the received modulation, so we called it broadband STI (bSTI). Its credibility with regard to signals altered by the environment or nonlinear speech changed by a DSP algorithm is checked by performing a comparative analysis of ten selected impulse responses for which a baseline value of STI was known.
    MeSH term(s) Algorithms ; Hearing ; Humans ; Noise ; Speech Intelligibility ; Speech Perception
    Language English
    Publishing date 2022-02-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s22041641
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  6. Article ; Online: Surgical interventions after emergency endovascular stent-grafting for acute type B aortic dissections.

    Duebener, Lennart / Hartmann, Franz / Kurowski, Volkhard / Richardt, Gert / Geist, Volker / Erasmi, Armin / Sievers, Hans-Hinrich / Misfeld, Martin

    Interactive cardiovascular and thoracic surgery

    2007  Volume 6, Issue 3, Page(s) 288–292

    Abstract: ... emergency placement of aortic stents for acute type B dissection. From October 2000 to June 2006 ... endovascular stent-grafting (ESG) was performed in 13 patients as an emergency procedure for acute type B ... emergency endovascular stent-grafting for acute type B dissection. Indications for surgery included acute ...

    Abstract In this retrospective study we reviewed our results of secondary surgery for complications after emergency placement of aortic stents for acute type B dissection. From October 2000 to June 2006, endovascular stent-grafting (ESG) was performed in 13 patients as an emergency procedure for acute type B dissection. Self-expanding nitinol stents (mean diameter 39.8+/-4.7 mm) were placed into the descending aorta distal to the left subclavian artery. In-hospital mortality was 15.4% (2/13) and related to persistent visceral malperfusion. Three patients (23%) required consecutive open surgery of the thoracic aorta after emergency endovascular stent-grafting for acute type B dissection. Indications for surgery included acute development of retrograde type A aortic dissection and acute stent dislocation by fractured wires and secondary leakage. Elective surgery was necessary in one patient 6 months after stent-grafting for late formation of an aneurysm of the descending aorta. There were no deaths or major morbidity after surgery of the thoracic aorta early or during follow-up. Mean follow-up was 38.0+/-13.9 months (range 1-70 months) and complete. We conclude from our study that stent-grafting of the descending aorta is a feasible, relatively safe and effective approach even in the emergency treatment of patients with complicated acute type B dissection. However, in a relevant number of patients emergency stent-grafting for acute type B aortic dissection results in complications that require secondary surgical treatment.
    MeSH term(s) Aged ; Alloys ; Aneurysm, Dissecting/diagnostic imaging ; Aneurysm, Dissecting/surgery ; Aortic Aneurysm, Thoracic/diagnostic imaging ; Aortic Aneurysm, Thoracic/surgery ; Blood Vessel Prosthesis Implantation ; Emergencies ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Stents ; Tomography, X-Ray Computed ; Treatment Outcome
    Chemical Substances Alloys ; nitinol (2EWL73IJ7F)
    Language English
    Publishing date 2007-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2095298-3
    ISSN 1569-9285 ; 1569-9293
    ISSN (online) 1569-9285
    ISSN 1569-9293
    DOI 10.1510/icvts.2006.144840
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    Zs.A 5730: Show issues Location:
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  7. Article ; Online: New strides towards fair processes for financing universal health coverage.

    Kurowski, Christoph / Evans, David B / Ottersen, Trygve / Gopinathan, Unni / Dale, Elina / Norheim, Ole Frithjof

    Health policy and planning

    2023  Volume 38, Issue Supplement_1, Page(s) i5–i8

    MeSH term(s) Humans ; Universal Health Insurance ; Health Care Reform ; Health Expenditures ; Healthcare Financing
    Language English
    Publishing date 2023-11-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 632896-9
    ISSN 1460-2237 ; 0268-1080
    ISSN (online) 1460-2237
    ISSN 0268-1080
    DOI 10.1093/heapol/czad065
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  8. Book ; Thesis: Medizinische Vorträge in der Lübecker Gesellschaft zur Beförderung Gemeinnütziger Tätigkeit 1789 - 1839

    Kurowski, Rüdiger

    eine patriotische Sozietät während der Aufklärung und Romantik

    (Veröffentlichungen zur Geschichte der Hansestadt Lübeck : Reihe B ; 25)

    1995  

    Author's details von Rüdiger Kurowski
    Series title Veröffentlichungen zur Geschichte der Hansestadt Lübeck : Reihe B ; 25
    Veröffentlichungen zur Geschichte der Hansestadt Lübeck
    Veröffentlichungen zur Geschichte der Hansestadt Lübeck ; Reihe B
    Collection Veröffentlichungen zur Geschichte der Hansestadt Lübeck
    Veröffentlichungen zur Geschichte der Hansestadt Lübeck ; Reihe B
    Keywords Societies / history / Lübeck ; History of Medicine, 18th Cent. / Lübeck ; History of Medicine, 19th Cent. / Lübeck ; Lübeck / societies / history ; Lübeck / history of medicine, 18th cent ; Lübeck / history of medicine, 19th cent ; Gesellschaft zur Beförderung Gemeinnütziger Tätigkeit ; Geschichte
    Language German
    Size 228 S. : Ill.
    Publisher Schmidt-Römhild
    Publishing place Lübeck
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Lübeck, Univ., Diss., 1993
    HBZ-ID HT006581127
    ISBN 3-7950-0463-2 ; 978-3-7950-0463-7
    Database Catalogue ZB MED Medicine, Health

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    1996 B 1924 order for loan Magazin

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  9. Article: Hyperglycemia inhibits insulin activation of Akt/protein kinase B but not phosphatidylinositol 3-kinase in rat skeletal muscle.

    Kurowski, T G / Lin, Y / Luo, Z / Tsichlis, P N / Buse, M G / Heydrick, S J / Ruderman, N B

    Diabetes

    1999  Volume 48, Issue 3, Page(s) 658–663

    Abstract: ... signaling in the latter muscles revealed that activation of Akt/protein kinase B (PKB) was diminished by 60 ...

    Abstract Sustained hyperglycemia impairs insulin-stimulated glucose utilization in the skeletal muscle of both humans and experimental animals--a phenomenon referred to clinically as glucose toxicity. To study how this occurs, a model was developed in which hyperglycemia produces insulin resistance in vitro. Rat extensor digitorum longus muscles were preincubated for 4 h in Krebs-Henseleit solution containing glucose or glucose + insulin at various concentrations, after which insulin action was studied. Preincubation with 25 mmol/l glucose + insulin (10 mU/ml) led to a 70% decrease in the ability of insulin (10 mU/ml) to stimulate glucose incorporation into glycogen and a 30% decrease in 2-deoxyglucose (2-DG) uptake, compared with muscles incubated with 0 mmol/l glucose. Glucose incorporation into lipid and its oxidation to CO2 were marginally diminished, if at all. The alterations of glycogen synthesis and 2-DG uptake were first evident after 1 h and were maximal after 2 h of preincubation; they were not observed in muscles preincubated with 25 mmol/l glucose + insulin for 5 min. Preincubation for 4 h with 25 mmol/l glucose in the absence of insulin produced a similar although somewhat smaller decrease in insulin-stimulated glycogen synthesis; however, it did not alter 2-DG uptake, glucose oxidation to CO2, or incorporation into lipids. Studies of insulin signaling in the latter muscles revealed that activation of Akt/protein kinase B (PKB) was diminished by 60%, compared with that of muscles preincubated in a glucose-free medium; whereas activation of phosphatidylinositol (PI) 3-kinase, an upstream regulator of Akt/PKB in the insulin-signaling cascade, and of mitogen-activated protein (MAP) kinase, a parallel signal, was unaffected. Immunoblots demonstrated that this was not due to a change in Akt/PKB abundance. The results indicate that hyperglycemia-induced insulin resistance can be studied in rat skeletal muscle in vitro. They suggest that impairment of insulin action in these muscles is related to inhibition of Akt/PKB by events that do not affect PI 3-kinase.
    MeSH term(s) Animals ; Calcium-Calmodulin-Dependent Protein Kinases/metabolism ; Deoxyglucose/metabolism ; Enzyme Activation ; Glucose/pharmacology ; Glycogen/biosynthesis ; Hyperglycemia/enzymology ; In Vitro Techniques ; Insulin/pharmacology ; Kinetics ; Male ; Mitogen-Activated Protein Kinase 1 ; Muscle, Skeletal/drug effects ; Muscle, Skeletal/enzymology ; Muscle, Skeletal/metabolism ; Phosphatidylinositol 3-Kinases/antagonists & inhibitors ; Phosphatidylinositol 3-Kinases/metabolism ; Protein-Serine-Threonine Kinases ; Protein-Tyrosine Kinases/metabolism ; Proto-Oncogene Proteins/antagonists & inhibitors ; Proto-Oncogene Proteins/metabolism ; Proto-Oncogene Proteins c-akt ; Rats ; Rats, Sprague-Dawley
    Chemical Substances Insulin ; Proto-Oncogene Proteins ; Glycogen (9005-79-2) ; Deoxyglucose (9G2MP84A8W) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; Akt1 protein, rat (EC 2.7.11.1) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Calcium-Calmodulin-Dependent Protein Kinases (EC 2.7.11.17) ; Mitogen-Activated Protein Kinase 1 (EC 2.7.11.24) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 1999-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 80085-5
    ISSN 1939-327X ; 0012-1797
    ISSN (online) 1939-327X
    ISSN 0012-1797
    DOI 10.2337/diabetes.48.3.658
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  10. Article: Polymyxin B inhibits contraction-stimulated glucose uptake in rat skeletal muscle.

    Young, J C / Kurowski, T G / Maurice, A M / Nesher, R / Ruderman, N B

    Journal of applied physiology (Bethesda, Md. : 1985)

    1991  Volume 70, Issue 4, Page(s) 1650–1654

    Abstract: ... in the postexercise state. Polymyxin B, a cyclic decapeptide antibiotic, inhibits the stimulation of glucose uptake ... muscle. The purpose of this study was to determine whether polymyxin B also inhibits contraction ... the effects of polymyxin B on tension development and its effects on contraction-stimulated glucose uptake ...

    Abstract Glucose transport in muscle is activated by contractile activity, an effect that persists in the postexercise state. Polymyxin B, a cyclic decapeptide antibiotic, inhibits the stimulation of glucose uptake in isolated muscle by contractile activity but also decreases tension development in electrically stimulated muscle. The purpose of this study was to determine whether polymyxin B also inhibits contraction-stimulated glucose uptake after in vivo administration of the drug and to examine the relationship between the effects of polymyxin B on tension development and its effects on contraction-stimulated glucose uptake. When polymyxin B was administered to rats in vivo, glucose uptake in muscle after electrical stimulation was decreased, despite the same amount of tension developed as in control rats, indicating an effect of polymyxin B on glucose transport independent of tension development. Our results also indicate that the postexercise increase in glucose uptake is a function of the tension developed by prior contractions. When muscles were perfused with medium containing polymyxin B, this relationship was disrupted. These results provide evidence that polymyxin B causes a decrease in muscle glucose uptake independent of its effects on tension development. The extent to which its effects on glucose uptake are also the result of a diminution in contractile force is uncertain.
    MeSH term(s) Animals ; Biological Transport, Active/drug effects ; Deoxyglucose/metabolism ; Electric Stimulation ; Glucose/metabolism ; In Vitro Techniques ; Male ; Muscle Contraction/drug effects ; Muscle Contraction/physiology ; Muscles/drug effects ; Muscles/metabolism ; Perfusion ; Polymyxin B/pharmacology ; Rats ; Rats, Inbred Strains
    Chemical Substances Polymyxin B (1404-26-8) ; Deoxyglucose (9G2MP84A8W) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 1991-04
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 219139-8
    ISSN 1522-1601 ; 8750-7587 ; 0021-8987 ; 0161-7567
    ISSN (online) 1522-1601
    ISSN 8750-7587 ; 0021-8987 ; 0161-7567
    Database MEDical Literature Analysis and Retrieval System OnLINE

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