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  1. Article ; Online: Perforated Amyand hernia with an adenocarcinoma tumour presenting as a groin abscess.

    Gbegli, Emmanuel / Miremadi, Ahmad / Serrao, Eva Mendes / Sadler, Timothy J

    BJR case reports

    2024  Volume 10, Issue 2, Page(s) uaae008

    Abstract: An Amyand hernia is an incarcerated inguinal hernia containing the appendix with or without appendicitis. This is a rare form of inguinal hernia, making up approximately 0.4%-1% of all cases. As with any hernia, this may become strangulated at any time, ... ...

    Abstract An Amyand hernia is an incarcerated inguinal hernia containing the appendix with or without appendicitis. This is a rare form of inguinal hernia, making up approximately 0.4%-1% of all cases. As with any hernia, this may become strangulated at any time, leading to the loss of blood supply and further development of gangrene and complications. Clinically, this can present in a manner indistinguishable from other types of inguinal hernias. In addition, the appendix can be affected by its own set of pathological processes, such as infection, inflammation, and malignancy. Not uncommonly both hernial and appendiceal complications coexist. The clinical diagnosis of an Amyand hernia remains challenging due to its low incidence and indistinct clinical presentation. At present, surgery is usually diagnostic and therapeutic. However, there is a growing number of recent reports showing the invaluable role of imaging on the diagnosis of Amyand hernias and associated complications. The correct and timely recognition of their imaging features including complications can optimize and expedite patient care by guiding diagnosis, treatment, and prognosis. Here, we report for the first time the radiological and pathological findings of a patient with a unique complicated Amyand hernia, which posed a diagnostic challenge for the clinical and radiological teams.
    Language English
    Publishing date 2024-03-06
    Publishing country England
    Document type Case Reports
    ISSN 2055-7159
    ISSN (online) 2055-7159
    DOI 10.1093/bjrcr/uaae008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Understanding the malignant potential of gastric metaplasia of the oesophagus and its relevance to Barrett's oesophagus surveillance: individual-level data analysis.

    Black, Emily L / Ococks, Emma / Devonshire, Ginny / Ng, Alvin Wei Tian / O'Donovan, Maria / Malhotra, Shalini / Tripathi, Monika / Miremadi, Ahmad / Freeman, Adam / Coles, Hannah / Fitzgerald, Rebecca C

    Gut

    2024  Volume 73, Issue 5, Page(s) 729–740

    Abstract: Objective: Whether gastric metaplasia (GM) of the oesophagus should be considered as Barrett's oesophagus (BO) is controversial. Given concern intestinal metaplasia (IM) may be missed due to sampling, the UK guidelines include GM as a type of BO. Here, ... ...

    Abstract Objective: Whether gastric metaplasia (GM) of the oesophagus should be considered as Barrett's oesophagus (BO) is controversial. Given concern intestinal metaplasia (IM) may be missed due to sampling, the UK guidelines include GM as a type of BO. Here, we investigated whether the risk of misdiagnosis and the malignant potential of GM warrant its place in the UK surveillance.
    Design: We performed a thorough pathology and endoscopy review to follow clinical outcomes in a novel UK cohort of 244 patients, covering 1854 person years of follow-up. We complemented this with a comparative genomic analysis of 160 GM and IM specimens, focused on early molecular hallmarks of BO and oesophageal adenocarcinoma (OAC).
    Results: We found that 58 of 77 short-segment (
    Conclusion: SS-GM is a distinct entity from SS-IM and the malignant potential of GM is lower than IM. It is questionable whether SS-GM warrants inclusion in BO surveillance.
    MeSH term(s) Humans ; Barrett Esophagus/diagnosis ; Barrett Esophagus/genetics ; Barrett Esophagus/complications ; Esophageal Neoplasms/diagnosis ; Esophageal Neoplasms/genetics ; Metaplasia ; Endoscopy, Gastrointestinal ; Adenocarcinoma
    Language English
    Publishing date 2024-04-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 80128-8
    ISSN 1468-3288 ; 0017-5749
    ISSN (online) 1468-3288
    ISSN 0017-5749
    DOI 10.1136/gutjnl-2023-330721
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: De novo distal terminal ileum adenocarcinoma mimicking Crohn's disease and diagnostic challenges in imaging: a case series.

    Lim, Dongwhee / Fernando, Suresh / Hyder, Syed / Malhotra, Shalini / Miremadi, Ahmad / Menon, Mukil

    BJR case reports

    2021  Volume 7, Issue 6, Page(s) 20210103

    Abstract: ... De ... ...

    Abstract De novo
    Language English
    Publishing date 2021-06-24
    Publishing country England
    Document type Case Reports
    ISSN 2055-7159
    ISSN (online) 2055-7159
    DOI 10.1259/bjrcr.20210103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole slide imaging with accurate single-cell segmentation.

    Kim, Eun Na / Chen, Phyllis Zixuan / Bressan, Dario / Tripathi, Monika / Miremadi, Ahmad / di Pietro, Massimiliano / Coussens, Lisa M / Hannon, Gregory J / Fitzgerald, Rebecca C / Zhuang, Lizhe / Chang, Young Hwan

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Imaging mass cytometry (IMC) is a powerful multiplexed tissue imaging technology that allows simultaneous detection of more than 30 makers on a single slide. It has been increasingly used for singlecell-based spatial phenotyping in a wide range of ... ...

    Abstract Imaging mass cytometry (IMC) is a powerful multiplexed tissue imaging technology that allows simultaneous detection of more than 30 makers on a single slide. It has been increasingly used for singlecell-based spatial phenotyping in a wide range of samples. However, it only acquires a small, rectangle field of view (FOV) with a low image resolution that hinders downstream analysis. Here, we reported a highly practical dual-modality imaging method that combines high-resolution immunofluorescence (IF) and high-dimensional IMC on the same tissue slide. Our computational pipeline uses the whole slide image (WSI) of IF as a spatial reference and integrates small FOVs IMC into a WSI of IMC. The high-resolution IF images enable accurate single-cell segmentation to extract robust high-dimensional IMC features for downstream analysis. We applied this method in esophageal adenocarcinoma of different stages, identified the single-cell pathology landscape via reconstruction of WSI IMC images, and demonstrated the advantage of the dual-modality imaging strategy.
    Motivation: Highly multiplexed tissue imaging allows visualization of the spatially resolved expression of multiple proteins at the single-cell level. Although imaging mass cytometry (IMC) using metal isotope-conjugated antibodies has a significant advantage of low background signal and absence of autofluorescence or batch effect, it has a low resolution that hampers accurate cell segmentation and results in inaccurate feature extraction. In addition, IMC only acquires mm
    Language English
    Publishing date 2023-02-23
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.23.529718
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation.

    Kim, Eun Na / Chen, Phyllis Zixuan / Bressan, Dario / Tripathi, Monika / Miremadi, Ahmad / di Pietro, Massimiliano / Coussens, Lisa M / Hannon, Gregory J / Fitzgerald, Rebecca C / Zhuang, Lizhe / Chang, Young Hwan

    Cell reports methods

    2023  Volume 3, Issue 10, Page(s) 100595

    Abstract: Imaging mass cytometry (IMC) is a powerful technique capable of detecting over 30 markers on a single slide. It has been increasingly used for single-cell-based spatial phenotyping in a wide range of samples. However, it only acquires a rectangle field ... ...

    Abstract Imaging mass cytometry (IMC) is a powerful technique capable of detecting over 30 markers on a single slide. It has been increasingly used for single-cell-based spatial phenotyping in a wide range of samples. However, it only acquires a rectangle field of view (FOV) with a relatively small size and low image resolution, which hinders downstream analysis. Here, we reported a highly practical dual-modality imaging method that combines high-resolution immunofluorescence (IF) and high-dimensional IMC on the same tissue slide. Our computational pipeline uses the whole-slide image (WSI) of IF as a spatial reference and integrates small-FOV IMC into a WSI of IMC. The high-resolution IF images enable accurate single-cell segmentation to extract robust high-dimensional IMC features for downstream analysis. We applied this method in esophageal adenocarcinoma of different stages, identified the single-cell pathology landscape via reconstruction of WSI IMC images, and demonstrated the advantage of the dual-modality imaging strategy.
    MeSH term(s) Humans ; Barrett Esophagus/pathology ; Esophageal Neoplasms/pathology ; Adenocarcinoma/diagnostic imaging ; Fluorescent Antibody Technique ; Image Cytometry
    Language English
    Publishing date 2023-09-22
    Publishing country United States
    Document type Journal Article
    ISSN 2667-2375
    ISSN (online) 2667-2375
    DOI 10.1016/j.crmeth.2023.100595
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Deciphering the Immune Complexity in Esophageal Adenocarcinoma and Pre-Cancerous Lesions With Sequential Multiplex Immunohistochemistry and Sparse Subspace Clustering Approach.

    Sundaram, Srinand / Kim, Eun Na / Jones, Georgina M / Sivagnanam, Shamilene / Tripathi, Monika / Miremadi, Ahmad / Di Pietro, Massimiliano / Coussens, Lisa M / Fitzgerald, Rebecca C / Chang, Young Hwan / Zhuang, Lizhe

    Frontiers in immunology

    2022  Volume 13, Page(s) 874255

    Abstract: Esophageal adenocarcinoma (EAC) develops from a chronic inflammatory environment across four stages: intestinal metaplasia, known as Barrett's esophagus, low- and high-grade dysplasia, and adenocarcinoma. Although the genomic characteristics of this ... ...

    Abstract Esophageal adenocarcinoma (EAC) develops from a chronic inflammatory environment across four stages: intestinal metaplasia, known as Barrett's esophagus, low- and high-grade dysplasia, and adenocarcinoma. Although the genomic characteristics of this progression have been well defined
    MeSH term(s) Adenocarcinoma/genetics ; Antigen-Antibody Complex ; Barrett Esophagus/pathology ; Cluster Analysis ; Ecosystem ; Esophageal Neoplasms ; Forkhead Transcription Factors ; Humans ; Immunohistochemistry ; Metaplasia ; Tumor Microenvironment
    Chemical Substances Antigen-Antibody Complex ; Forkhead Transcription Factors
    Language English
    Publishing date 2022-05-19
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.874255
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Feasibility of combined screening for upper gastrointestinal adenocarcinoma risk by serology and Cytosponge testing: the SUGAR study.

    Xu, Yiwang / Miremadi, Ahmad / Link, Alexander / Malfertheiner, Peter / Fitzgerald, Rebecca C / Bornschein, Jan

    Journal of clinical pathology

    2019  Volume 72, Issue 12, Page(s) 825–829

    Abstract: Aims: Aim was to assess the feasibility of serum markers to identify individuals at risk for gastro-oesophageal adenocarcinoma to reduce the number of individuals requiring invasive assessment by endoscopy.: Methods: Blood samples from 56 patients ... ...

    Abstract Aims: Aim was to assess the feasibility of serum markers to identify individuals at risk for gastro-oesophageal adenocarcinoma to reduce the number of individuals requiring invasive assessment by endoscopy.
    Methods: Blood samples from 56 patients with Barrett's oesophagus and 202 non-Barrett controls who previously took part in a trial assessing the accuracy of the Cytosponge for Barrett's oesophagus were assessed for serum pepsinogen (PG) 1 and 2, gastrin-17, trefoil factor 3 (TFF3) and
    Results: PG1 was pathological (<50 ng/mL) in 26 patients (10.1%), none of whom had Barrett's oesophagus (p<0.001). Smoking and drinking had no influence on these results. Pathological PG1 was associated with stomach pain (p=0.029), disruption of sleep (p=0.027) and disruption of diet by symptoms (p=0.019). Serum TFF3 was not associated with any clinical parameter.
    Conclusions: Assessment of serum PG1 could be combined with a test for Barrett's oesophagus to identify additional patients requiring endoscopy.
    MeSH term(s) Adenocarcinoma/blood ; Adenocarcinoma/epidemiology ; Adenocarcinoma/pathology ; Adult ; Aged ; Aged, 80 and over ; Barrett Esophagus/blood ; Barrett Esophagus/epidemiology ; Barrett Esophagus/pathology ; Biomarkers, Tumor/blood ; Case-Control Studies ; Early Detection of Cancer/instrumentation ; Early Detection of Cancer/methods ; Esophageal Neoplasms/blood ; Esophageal Neoplasms/epidemiology ; Esophageal Neoplasms/pathology ; Feasibility Studies ; Female ; Humans ; Male ; Middle Aged ; Pepsinogen A/blood ; Pepsinogen C/blood ; Predictive Value of Tests ; Prognosis ; Reproducibility of Results ; Risk Assessment ; Risk Factors ; Serologic Tests ; Specimen Handling/instrumentation ; Stomach Neoplasms/blood ; Stomach Neoplasms/epidemiology ; Stomach Neoplasms/pathology ; Trefoil Factor-3/analysis ; Young Adult
    Chemical Substances Biomarkers, Tumor ; TFF3 protein, human ; Trefoil Factor-3 ; Pepsinogen C (61536-72-9) ; Pepsinogen A (9001-10-9)
    Language English
    Publishing date 2019-06-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 80261-x
    ISSN 1472-4146 ; 0021-9746
    ISSN (online) 1472-4146
    ISSN 0021-9746
    DOI 10.1136/jclinpath-2019-205700
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The utility of P53 immunohistochemistry in the diagnosis of Barrett's oesophagus with indefinite for dysplasia.

    Januszewicz, Wladyslaw / Pilonis, Nastazja D / Sawas, Tarek / Phillips, Richard / O'Donovan, Maria / Miremadi, Ahmad / Malhotra, Shalini / Tripathi, Monika / Blasko, Adrienn / Katzka, David A / Fitzgerald, Rebecca C / di Pietro, Massimiliano

    Histopathology

    2022  Volume 80, Issue 7, Page(s) 1081–1090

    Abstract: Aims: Barrett's oesophagus with indefinite for dysplasia (BE-IND) is a subjective diagnosis with a low interobserver agreement (IOA) among pathologists and uncertain clinical implications. This study aimed to assess the utility of p53 ... ...

    Abstract Aims: Barrett's oesophagus with indefinite for dysplasia (BE-IND) is a subjective diagnosis with a low interobserver agreement (IOA) among pathologists and uncertain clinical implications. This study aimed to assess the utility of p53 immunohistochemistry (p53-IHC) in assessing BE-IND specimens.
    Methods and results: Archive endoscopic biopsies with a BE-IND diagnosis from two academic centres were analysed. First, haematoxylin and eosin-stained slides (H&E) were reviewed by four expert gastrointestinal (GI) pathologists allocated into two groups (A and B). After a washout period of at least 8 weeks, H&E slides were reassessed side-to-side with p53-IHC available. We compared the rate of changed diagnosis and the IOA for all BE grades before and after p53-IHC. We included 216 BE-IND specimens from 185 patients, 44.0 and 32.9% of which were confirmed after H&E slide revision by groups A and B, respectively. More than half the cases were reclassified to a non-dysplastic BE (NDBE), while 5.6% of cases in group A and 7.4% in group B were reclassified to definite dysplasia. The IOA for NDBE, BE-IND, low-grade dysplasia (LGD) and high-grade dysplasia (HGD)/intramucosal cancer (IMC) was 0.31, 0.21, -0.03 and -0.02, respectively. Use of p53-IHC led to a >40% reduction in BE-IND diagnoses (P < 0.001) and increased IOA for all BE grades [κ = 0.46 (NDBE), 0.26 (BE-IND), 0.49 (LGD), 0.35 (HGD/IMC)]. An aberrant p53-IHC pattern significantly increased the likelihood of reclassifying BE-IND to definite dysplasia (odds ratio = 44.3, 95% confidence interval = 18.8-113.0).
    Conclusion: P53-IHC reduces the rate of BE-IND diagnoses and improves the IOA among pathologists when reporting BE with equivocal epithelial changes.
    MeSH term(s) Barrett Esophagus/diagnosis ; Barrett Esophagus/pathology ; Disease Progression ; Esophageal Neoplasms/diagnosis ; Esophageal Neoplasms/pathology ; Humans ; Hyperplasia ; Immunohistochemistry ; Precancerous Conditions/diagnosis ; Precancerous Conditions/pathology ; Tumor Suppressor Protein p53
    Chemical Substances Tumor Suppressor Protein p53
    Language English
    Publishing date 2022-03-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 131914-0
    ISSN 1365-2559 ; 0309-0167
    ISSN (online) 1365-2559
    ISSN 0309-0167
    DOI 10.1111/his.14642
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Low-cost and clinically applicable copy number profiling using repeat DNA.

    Abujudeh, Sam / Zeki, Sebastian S / van Lanschot, Meta C J / Pusung, Mark / Weaver, Jamie M J / Li, Xiaodun / Noorani, Ayesha / Metz, Andrew J / Bornschein, Jan / Bower, Lawrence / Miremadi, Ahmad / Fitzgerald, Rebecca C / Morrissey, Edward R / Lynch, Andy G

    BMC genomics

    2022  Volume 23, Issue 1, Page(s) 599

    Abstract: Background: Somatic copy number alterations (SCNAs) are an important class of genomic alteration in cancer. They are frequently observed in cancer samples, with studies showing that, on average, SCNAs affect 34% of a cancer cell's genome. Furthermore, ... ...

    Abstract Background: Somatic copy number alterations (SCNAs) are an important class of genomic alteration in cancer. They are frequently observed in cancer samples, with studies showing that, on average, SCNAs affect 34% of a cancer cell's genome. Furthermore, SCNAs have been shown to be major drivers of tumour development and have been associated with response to therapy and prognosis. Large-scale cancer genome studies suggest that tumours are driven by somatic copy number alterations (SCNAs) or single-nucleotide variants (SNVs). Despite the frequency of SCNAs and their clinical relevance, the use of genomics assays in the clinic is biased towards targeted gene panels, which identify SNVs but provide limited scope to detect SCNAs throughout the genome. There is a need for a comparably low-cost and simple method for high-resolution SCNA profiling.
    Results: We present conliga, a fully probabilistic method that infers SCNA profiles from a low-cost, simple, and clinically-relevant assay (FAST-SeqS). When applied to 11 high-purity oesophageal adenocarcinoma samples, we obtain good agreement (Spearman's rank correlation coefficient, r
    Conclusions: We show that conliga provides high-resolution SCNA profiles using a convenient, low-cost assay. We believe conliga makes FAST-SeqS a more clinically valuable assay as well as a useful research tool, enabling inexpensive and fast copy number profiling of pre-malignant and cancer samples.
    MeSH term(s) Base Sequence ; DNA ; DNA Copy Number Variations ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Neoplasms/genetics
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2022-08-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041499-7
    ISSN 1471-2164 ; 1471-2164
    ISSN (online) 1471-2164
    ISSN 1471-2164
    DOI 10.1186/s12864-022-08681-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: An Analysis of GLOBE’s Organization Culture on Work Ethics

    Alireza Miremadi / Syed Reza Jalili / Ahmad Sharbatoghile

    International Business and Management, Vol 10, Iss 1, Pp 55-

    A Case Study of Iran

    2015  Volume 61

    Abstract: The purpose of this research is to express the importance of ethics in business and also the expression of organizational culture dimensions (GLOBE) and the power of organizational culture in institutionalizing ethics in business as an efficient ... ...

    Abstract The purpose of this research is to express the importance of ethics in business and also the expression of organizational culture dimensions (GLOBE) and the power of organizational culture in institutionalizing ethics in business as an efficient competitive advantage. This study seeks to find a relationship between organizational culture dimensions and implementation of business ethics and also the most important organizational culture dimension in improving ethics in business. The research methodology is descriptive based on data collection method. For this purpose, the correlation method was used to describe the research variables and explain relationships. The research questionnaire was done in the temporal domain until winter 2014 in the spatial domain of Social Security Organization of Golpayegan (as the mother organization, Khansar and Daran, all of which located in Isfahan Province. The Study reveals that the significance probability value of each regression independent variable shows that only variables “Power Distance, Gender Egalitarianism, Future Orientation, and Human Orientation”, due to P-value Considering the results of the research, it’s suggested that a senior manager intending to revive the business ethics in his organization by means of the powerful leverage of organizational culture, should invest on power distance, gender equality, foresight and altruism more. Key words: Business ethics; Organizational culture; GLOBE; Nobel Quran
    Keywords Business ethics ; Organizational culture ; GLOBE ; Nobel Quran ; Business ; HF5001-6182 ; Commerce ; HF1-6182 ; Social Sciences ; H
    Subject code 170
    Language English
    Publishing date 2015-02-01T00:00:00Z
    Publisher Canadian Research & Development Center of Sciences and Cultures
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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