Article: Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1 alpha.
2005 Volume 23, Issue 3, Page(s) 383–391
Abstract: Mesenchymal stem cells (MSCs) exhibit immune-suppressive properties, follow a pattern of multilineage differentiation, and exhibit transdifferentiation potential. Ease in expansion from adult bone marrow, as well as its separation from ethical issues, ... ...
| Abstract | Mesenchymal stem cells (MSCs) exhibit immune-suppressive properties, follow a pattern of multilineage differentiation, and exhibit transdifferentiation potential. Ease in expansion from adult bone marrow, as well as its separation from ethical issues, makes MSCs appealing for clinical application. MSCs treated with retinoic acid resulted in synaptic transmission, based on immunostaining of synaptophysin and electrophysiological studies. In situ hybridization indicated that the neurotransmitter gene preprotachykinin-I was expressed in these cells. However, translation of this gene only occurred after stimulation with interleukin (IL)-1 alpha. This effect was blunted by costimulation with IL-1 receptor antagonist. This study reports on the ability of MSCs to be transdifferentiated into neurons with functional synapses with the potential to become polarized towards producing specific neurotransmitters. |
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| MeSH term(s) | Bone Marrow Cells/cytology ; Cell Differentiation/drug effects ; Electrophysiology ; Gene Expression/drug effects ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Interleukin 1 Receptor Antagonist Protein ; Interleukin-1/pharmacology ; Intermediate Filament Proteins/metabolism ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/metabolism ; Microtubule-Associated Proteins/metabolism ; Nerve Tissue Proteins/metabolism ; Nestin ; Neurofilament Proteins/metabolism ; Neurons/cytology ; Neurons/metabolism ; Neurons/physiology ; Protein Precursors/genetics ; Sialoglycoproteins/pharmacology ; Substance P/metabolism ; Synaptic Transmission/physiology ; Synaptophysin/metabolism ; Tachykinins/genetics ; Tretinoin/pharmacology |
| Chemical Substances | IL1RN protein, human ; Interleukin 1 Receptor Antagonist Protein ; Interleukin-1 ; Intermediate Filament Proteins ; MAP2 protein, human ; Microtubule-Associated Proteins ; NES protein, human ; Nerve Tissue Proteins ; Nestin ; Neurofilament Proteins ; Protein Precursors ; Sialoglycoproteins ; Synaptophysin ; Tachykinins ; preprotachykinin ; Substance P (33507-63-0) ; Tretinoin (5688UTC01R) |
| Language | English |
| Publishing date | 2005-03 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 1143556-2 |
| ISSN | 1549-4918 ; 1066-5099 |
| ISSN (online) | 1549-4918 |
| ISSN | 1066-5099 |
| DOI | 10.1634/stemcells.2004-0251 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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