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  1. Article: Sravnitel'noe laboratornoe issledovanie rezul'tatov otobrazheniya granitsy preparirovaniya kul'ti zuba, poluchennykh s pomoshch'yu intraoral'nykh skanerov.

    Ryakhovskiy, A N / Kostyukova, V V

    Stomatologiia

    2016  Volume 95, Issue 5, Page(s) 39–46

    Abstract: ... models with prepared teeth were scanned using intraoral scanning systems: 3D Progress (MHT S.P ... A., IT - MHT Optic Research AG, CH); True Definition (3M ESPE, USA); Trios (3Shape A/S, DNK); CEREC AC Bluecam ...

    Title translation Comparative analysis of 3D data visibility of the prepared tooth finishing line on a synthetic jaw model, captured by international scanners in a laboratory conditions.
    Abstract The aim of the study was to compare accuracy of digital impression's finishing line and the zone under it taken by different intraoral scanning systems. Parameters of comparison were: different level of the finishing line to the gingiva and width of sulcus after retraction. For this purpose two synthetic jaw models with prepared teeth were scanned using intraoral scanning systems: 3D Progress (MHT S.P.A., IT - MHT Optic Research AG, CH); True Definition (3M ESPE, USA); Trios (3Shape A/S, DNK); CEREC AC Bluecam, CEREC Omnicam (Sirona Dental System GmbH, DE); Planscan (Planmeca, FIN) (each n=10). Reference-scanning was done by ATOS Core (GOM mbH, DE). The resulting digital impressions were superimposed with the master-scan. The lowest measured deviations (trueness) for intraoral scanners, where the finishing line was 0.5 mm above gingiva were with scanner True Definition - 18.8±6.63 (on the finishing line) and 51.0±14.33 µm (0.3 mm under the finishing line). In conditions where finishing line was on the same level with gingiva, scanner Trios showed the best results: 17.0±3.96 and 52.7±6.52 µm. When the finishing line was 0.5 mm under gingiva, none of the testing scanners could visualize the zone 0.3 mm lower the finishing line. The best results for accuracy o the finishing line in that circumstances showed Trios: 15.1±5.05 µm. The optimum visualization of the finishing line and the zone under it was reached when the sulcus was 0.3 mm after retraction. Thus, the best accuracy was obtained with Trios: 10.3±2.69 (on the finishing line) and 57.2±13.58 µm (0.3 mm under finishing line). The results show that intraoral scanners also provide enough accuracy for indicating finishing line and the zone under it in different conditions of preparation and gingiva retraction. However, not all of the testing scanners can properly indicate finishing line and the zone under it when shoulder is below gingiva and the width of sulcus is less than 0.2 mm.
    MeSH term(s) Dental Impression Technique/instrumentation ; Dental Models ; Gingiva ; Gingival Retraction Techniques ; Humans ; Imaging, Three-Dimensional/instrumentation ; Jaw/anatomy & histology ; Periodontium ; Tooth Preparation/methods
    Language Russian
    Publishing date 2016
    Publishing country Russia (Federation)
    Document type Comparative Study ; Journal Article
    ZDB-ID 421717-2
    ISSN 0039-1735
    ISSN 0039-1735
    DOI 10.17116/stomat201695539-46
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  2. Article ; Online: Digitalization of the Japanese Economy by the Example of the Banking Sector

    K. S. Kostyukova

    Модернизация, инновация, развитие, Vol 12, Iss 4, Pp 434-

    Current Results, Prospects and Problems

    2022  Volume 449

    Abstract: Purpose: the purpose of this study is to examine the current state of Japan's digital transformation, with a focus on the banking sector, analyzing specifc examples, identifying obstacles that hinder the achievement of expected results.Methods: the ... ...

    Abstract Purpose: the purpose of this study is to examine the current state of Japan's digital transformation, with a focus on the banking sector, analyzing specifc examples, identifying obstacles that hinder the achievement of expected results.Methods: the article is based on the analysis of scientifc and analytical materials devoted to the problem of research, a set of general scientific methods is applied, including comparison, scientifc generalization, analogy, etc.Results: the article presents the main trends and current results of digitalization of Japanese banks, provides examples of projects, identifies problematic areas of the implemented policy. A hypothesis is put forward about the key role of the government in the development of new standards for the organization of work, taking into account the use of innovative technologies. For Japan, digitalization is chosen as a key tool to mitigate the current socio-economic situation. Digitalization is already being implemented in the healthcare sector, agriculture, etc. Another sector at the center of digitalization is the fnancial one. Financial technologies form new markets and products. Non-banking companies penetrate into the areas occupied by classical banks, competing and even displacing them.These processes are observed both in Japan and in other countries of the world, including Russia. Therefore, studying the Japanese experience of integrating digital technologies into the fnancial sector is important. Although Russia is among the leaders in the digitalization of the financial sector, it is difcult for fntech companies to break into the Russian market due to pressure from large classical banks, as well as the lack of trust in fntech companies from the population.Conclusions and Relevance: in contrast to the manufacturing sector, digitalization of non-manufacturing areas is slower in Japan. This is due to a shortage of qualifed personnel, fnancial risks, etc. Today, the priority for fnancial institutions remains to create the basis for the introduction of ...
    Keywords цифровая трансформация ; цифровизация банков ; финтех ; цифровые платформы ; большие данные ; искусственный интеллект ; научно-техническая политика японии ; Economics as a science ; HB71-74
    Subject code 950
    Language Russian
    Publishing date 2022-01-01T00:00:00Z
    Publisher Nauka
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Nuclear Magnetic Resonance-Guided Structural Analysis of Moderate-Affinity Protein Complexes with Intrinsically Disordered Polypeptides.

    Tolkatchev, Dmitri / Smith, Garry E / Kostyukova, Alla S

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2652, Page(s) 405–437

    Abstract: Binding affinity of an individual binding site of an intrinsically disordered protein for its folded partner may be moderate. In such cases, a straightforward determination of the structure of the binding interface is difficult. We offer a hybrid ... ...

    Abstract Binding affinity of an individual binding site of an intrinsically disordered protein for its folded partner may be moderate. In such cases, a straightforward determination of the structure of the binding interface is difficult. We offer a hybrid protocol combining NMR chemical shift information, NMR spectral data on amino acid residue sequence substitution effects, residual dipolar coupling, and molecular dynamics simulation that allowed us to determine the structure of a complex between the intrinsically disordered tropomyosin-binding site of leiomodin and a coiled-coil peptide modeling the N-terminal fragment of tropomyosin. The protocol can be used for other moderate-affinity complexes composed of an intrinsically disordered peptide bound to a structured protein partner.
    MeSH term(s) Tropomyosin/chemistry ; Tropomyosin/metabolism ; Nuclear Magnetic Resonance, Biomolecular/methods ; Magnetic Resonance Spectroscopy ; Protein Binding ; Intrinsically Disordered Proteins/chemistry ; Peptides/metabolism
    Chemical Substances Tropomyosin ; Intrinsically Disordered Proteins ; Peptides
    Language English
    Publishing date 2023-04-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3147-8_23
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  4. Article ; Online: Digitalization of Agriculture Sector in Japan

    K. S. Kostyukova

    Modernizaciâ, Innovaciâ, Razvitie, Vol 11, Iss 4, Pp 358-

    2020  Volume 369

    Abstract: Purpose: this article includes the review of the Japanese policy on the digital transformation transformation of the agricultural sector, description and analysis of the implementation cases, as well as identification of obstacles to achieve the expected ...

    Abstract Purpose: this article includes the review of the Japanese policy on the digital transformation transformation of the agricultural sector, description and analysis of the implementation cases, as well as identification of obstacles to achieve the expected results.Methods: the study uses the methods of structural, comparative and systems analysis and the principle of formal logic. The article is based on the analysis of scientific and analytical materials devoted to the problem of research. The factual basis is the framework documents of the Cabinet of Japan, media, reports of Japanese research institutes.Results: the article provides a brief review and analysis of the Japanese public of digital transformation of the agricultural sector, provides some cases, summarizes the interim results of the measures taken, identifies problematic factors that prevent the achievement of the expected results. The hypothesis is put forward about the key role of the government in the process of developing new standards for the digital transformation.Conclusions and Relevance: Japan agricultural sector is facing a severe labor shortage. The situation is expected to get worse in the next 5 years. The Japanese government expects that digital technologies and autonomous equipment will compensate for the lack of labor on farms, as well as provide a new stage in the development of the traditional agricultural sector. However, the digitalization of agriculture sector is not limited to the introduction of the latest technology, but also involves the development of new safety rules for the use of robotic tractors, unmanned aerial vehicles, improving farmers ICT education, and standardizing new agricultural terms. However, despite the promise of using digital technologies, the high cost of new equipment and the complexity of its use is a significant obstacle to the digitalization of agriculture in Japan. The study of the features of the digital transformation of the Japanese agricultural sector is very important for the effective ...
    Keywords digital transformation ; digitalization of agriculture sector ; digital platforms ; big data ; internet of things ; artificial intelligence ; autonomous machines ; drones ; science and technology policy of japan ; Economics as a science ; HB71-74
    Subject code 028
    Language Russian
    Publishing date 2020-12-01T00:00:00Z
    Publisher Nauka
    Document type Article ; Online
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  5. Article ; Online: The role of leiomodin in actin dynamics: a new road or a secret gate.

    Tolkatchev, Dmitri / Gregorio, Carol C / Kostyukova, Alla S

    The FEBS journal

    2021  Volume 289, Issue 20, Page(s) 6119–6131

    Abstract: Leiomodin is an important emerging regulator of thin filaments. As novel molecular, cellular, animal model, and human data accumulate, the mechanisms of its action become clearer. Structural studies played a significant part in understanding the ... ...

    Abstract Leiomodin is an important emerging regulator of thin filaments. As novel molecular, cellular, animal model, and human data accumulate, the mechanisms of its action become clearer. Structural studies played a significant part in understanding the functional significance of leiomodin's interacting partners and functional domains. In this review, we present the current state of knowledge on the structural and cellular properties of leiomodin which has led to two proposed mechanisms of its function. Although it is known that leiomodin is essential for life, numerous domains within leiomodin remain unstudied and as such, we outline future directions for investigations that we predict will provide evidence that leiomodin is a multifunctional protein.
    MeSH term(s) Actin Cytoskeleton/metabolism ; Actins/metabolism ; Animals ; Binding Sites ; Humans ; Tropomodulin/metabolism ; Tropomyosin/chemistry
    Chemical Substances Actins ; Tropomodulin ; Tropomyosin
    Language English
    Publishing date 2021-07-26
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2173655-8
    ISSN 1742-4658 ; 1742-464X
    ISSN (online) 1742-4658
    ISSN 1742-464X
    DOI 10.1111/febs.16128
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  6. Article: Role of Tropomodulin’s Leucine Rich Repeat Domain in the Formation of Neurite-like Processes

    Guillaud, Laurent / Gray Kevin T / Moroz Natalia / Pantazis Caroline / Pate Edward / Kostyukova Alla S

    Biochemistry. 2014 Apr. 29, v. 53, no. 16

    2014  

    Abstract: ... the role of Tmod’s C-terminal (LRR) domain in the formation of neurite-like processes by overexpressing ... of conserved residues. Removal of Tmod2’s LRR domain does not significantly alter the outgrowth of neurite ... domains switched between Tmod1 and Tmod2 reinforces the idea that Tmod1’s LRR domain counteracts ...

    Abstract Actin dynamics is fundamental for neurite development; monomer depolymerization from pointed ends is rate-limiting in actin treadmilling. Tropomodulins (Tmod) make up a family of actin pointed end-capping proteins. Of the four known isoforms, Tmod1–Tmod3 are expressed in brain cells. We investigated the role of Tmod’s C-terminal (LRR) domain in the formation of neurite-like processes by overexpressing Tmod1 and Tmod2 with deleted or mutated LRR domains in PC12 cells, a model system used to study neuritogenesis. Tmod1 overexpression results in a normal quantity and a normal length of processes, while Tmod2 overexpression reduces both measures. The Tmod2 overexpression phenotype is mimicked by overexpression of Tmod1 with the LRR domain removed or with three point mutations in the LRR domain that disrupt exposed clusters of conserved residues. Removal of Tmod2’s LRR domain does not significantly alter the outgrowth of neurite-like processes compared to that of Tmod2. Overexpression of chimeras with the N-terminal and C-terminal domains switched between Tmod1 and Tmod2 reinforces the idea that Tmod1’s LRR domain counteracts the reductive effect of the Tmod N-terminal domain upon formation of processes while Tmod2’s LRR domain does not. We suggest that the TM-dependent actin capping ability of both Tmods inhibits the formation of processes, but in Tmod1, this inhibition can be controlled via its LRR domain. Circular dichroism, limited proteolysis, and molecular dynamics demonstrate structural differences in the C-terminal region of the LRR domains of Tmod1, Tmod2, and the Tmod1 mutant.
    Keywords actin ; brain ; chimerism ; circular dichroism spectroscopy ; depolymerization ; gene overexpression ; leucine ; molecular dynamics ; mutants ; neurites ; phenotype ; point mutation ; proteolysis
    Language English
    Dates of publication 2014-0429
    Size p. 2689-2700.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021%2Fbi401431k
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Digital Transformation Policy in Japan

    K. S. Kostyukova

    Модернизация, инновация, развитие, Vol 10, Iss 4, Pp 516-

    the Case of Artificial Intelligence

    2019  Volume 529

    Abstract: Purpose: this article includes the review of the Japanese policy on the national economy Digital Transformation, using the case of implementation of artificial intelligence for production and services, description and analysis of the current results, as ... ...

    Abstract Purpose: this article includes the review of the Japanese policy on the national economy Digital Transformation, using the case of implementation of artificial intelligence for production and services, description and analysis of the current results, as well as identification of obstacles to achieve the expected results. Methods: the article is based on the analysis of scientific and analytical materials to the problem of research. The factual basis is the framework documents of the Cabinet of Japan, media, reports on research conducted by Japanese research institutes. Results: the article provides a brief review and analysis of the Japanese public policy of the development of AI technology, summarizes the interim results of the measures taken, identifies problematic factors that prevent the achievement of the expected results. The hypothesis is put forward about the insufficiency of government efforts to monitor and analyze the activities already implemented, the lack of practice of taking into account failures in the formation of new programs and projects. Recently, significant attention to the development of AI technology is paid in Russia. In 2019, Russian President Vladimir Putin approved the "National strategy for the development of AI until 2030". It is expected that the using of digital technologies, will increase the competitiveness of the national economy, improve the welfare of society. In this regard, the study of Japan's experience as one of the technological leaders in the development and using of digital transformation, is especially important. Conclusions and Relevance: To solve the current socio-economic problems, Japan government relies on the development of the advanced technologies. At the same time, the government continues to develop measures to stimulate the cooperation of the academic and industrial sectors to conduct joint R&D on priority technologies. However, due to the "closed" nature of Japanese corporations and the relative independence of government plans in determining the ...
    Keywords цифровая трансформация ; абэномика ; искусственный интеллект ; роботизация и автоматизация производства ; научно-техническая политика японии ; Economics as a science ; HB71-74
    Subject code 950
    Language Russian
    Publishing date 2019-12-01T00:00:00Z
    Publisher Nauka
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Increasing the "Productivity" of the Academic Sector and Ensuring Cooperation with the Large Corporate Sector in Japan

    K. S. Kostyukova

    Модернизация, инновация, развитие, Vol 9, Iss 4, Pp 657-

    2019  Volume 670

    Abstract: Purpose: the main purpose of this article is to review the Japanese policy to promote cooperation between the academic and large corporate sectors in the field of science and technology, and to analyze its role in the formation of the innovation system ... ...

    Abstract Purpose: the main purpose of this article is to review the Japanese policy to promote cooperation between the academic and large corporate sectors in the field of science and technology, and to analyze its role in the formation of the innovation system in Japan.Methods: this article is based on the analysis of scientific and analytical materials on the problem of research. The factual basis is the framework documents of the Cabinet of Japan.Results: the article provides a brief retrospective review on the support policy for universities and large corporate sector cooperation, identifies the key factors for the establishment of intersectoral cooperation; identifies the problem areas of the implemented policy that hinder the achievement of the set goals. The relative failure of the applied measures in the sphere of venture business support, commercialization of technologies and other instruments was noted. The hypothesis is put forward about the insufficiency of government efforts to monitor and analyze the activities already implemented, the lack of practice of taking into account failures in the formation of new programs and projects. Also revealed contradictions between the support programs implemented through various government agencies, which also leads to the inefficiency of scientific and technological projects and the establishment of dialogue with the corporate sector, including the need to resolve bureaucratic disagreements. It is also necessary to emphasize, that Japanese experience in implementing of science and technology and innovation policy is very important for Russian economic development. In particular, special attention is paid to study foreign experience in creating an effective mechanism of academic and private sectors interaction, where the key-supporting role is played by government in case of Japan. No doubt, it is not justified to try to copy any foreign institutional system, but the analysis of the negative and positive effects of foreign economic system cases is very valuable for ...
    Keywords абэномика ; партнерство академического и корпоративного сектора ; межсекторальное взаимодействие ; стратегия возрождения японии ; национальная инновационная система ; Economics as a science ; HB71-74
    Subject code 950
    Language Russian
    Publishing date 2019-01-01T00:00:00Z
    Publisher Nauka
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: A nemaline myopathy-linked mutation inhibits the actin-regulatory functions of tropomodulin and leiomodin.

    Schultz, Lauren E / Colpan, Mert / Smith, Garry E / Mayfield, Rachel M / Larrinaga, Tania M / Kostyukova, Alla S / Gregorio, Carol C

    Proceedings of the National Academy of Sciences of the United States of America

    2023  Volume 120, Issue 47, Page(s) e2315820120

    Abstract: Actin is a highly expressed protein in eukaryotic cells and is essential for numerous cellular processes. In particular, efficient striated muscle contraction is dependent upon the precise regulation of actin-based thin filament structure and function. ... ...

    Abstract Actin is a highly expressed protein in eukaryotic cells and is essential for numerous cellular processes. In particular, efficient striated muscle contraction is dependent upon the precise regulation of actin-based thin filament structure and function. Alterations in the lengths of actin-thin filaments can lead to the development of myopathies. Leiomodins and tropomodulins are members of an actin-binding protein family that fine-tune thin filament lengths, and their dysfunction is implicated in muscle diseases. An Lmod3 mutation [G326R] was previously identified in patients with nemaline myopathy (NM), a severe skeletal muscle disorder; this residue is conserved among Lmod and Tmod isoforms and resides within their homologous leucine-rich repeat (LRR) domain. We mutated this glycine to arginine in Lmod and Tmod to determine the physiological function of this residue and domain. This G-to-R substitution disrupts Lmod and Tmod's LRR domain structure, altering their binding interface with actin and destroying their abilities to regulate thin filament lengths. Additionally, this mutation renders Lmod3 nonfunctional in vivo. We found that one single amino acid is essential for folding of Lmod and Tmod LRR domains, and thus is essential for the opposing actin-regulatory functions of Lmod (filament elongation) and Tmod (filament shortening), revealing a mechanism underlying the development of NM.
    MeSH term(s) Humans ; Actins/metabolism ; Tropomodulin/genetics ; Tropomodulin/metabolism ; Myopathies, Nemaline/genetics ; Myopathies, Nemaline/metabolism ; Muscle Proteins/metabolism ; Actin Cytoskeleton/genetics ; Actin Cytoskeleton/metabolism ; Sarcomeres/genetics ; Sarcomeres/metabolism ; Mutation ; Muscle, Skeletal/metabolism
    Chemical Substances Actins ; Tropomodulin ; Muscle Proteins
    Language English
    Publishing date 2023-11-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2315820120
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  10. Article ; Online: Effects of Tropomodulin 2 on Dendritic Spine Reorganization and Dynamics.

    Kuruba, Balaganesh / Starks, Nickolas / Josten, Mary Rose / Naveh, Ori / Wayman, Gary / Mikhaylova, Marina / Kostyukova, Alla S

    Biomolecules

    2023  Volume 13, Issue 8

    Abstract: Dendritic spines are actin-rich protrusions that receive a signal from the axon at the synapse. Remodeling of cytoskeletal actin is tightly connected to dendritic spine morphology-mediated synaptic plasticity of the neuron. Remodeling of cytoskeletal ... ...

    Abstract Dendritic spines are actin-rich protrusions that receive a signal from the axon at the synapse. Remodeling of cytoskeletal actin is tightly connected to dendritic spine morphology-mediated synaptic plasticity of the neuron. Remodeling of cytoskeletal actin is required for the formation, development, maturation, and reorganization of dendritic spines. Actin filaments are highly dynamic structures with slow-growing/pointed and fast-growing/barbed ends. Very few studies have been conducted on the role of pointed-end binding proteins in the regulation of dendritic spine morphology. In this study, we evaluated the role played by tropomodulin 2 (Tmod2)-a brain-specific isoform, on the dendritic spine re-organization. Tmod2 regulates actin nucleation and polymerization by binding to the pointed end via actin and tropomyosin (Tpm) binding sites. We studied the effects of Tmod2 overexpression in primary hippocampal neurons on spine morphology using confocal microscopy and image analysis. Tmod2 overexpression decreased the spine number and increased spine length. Destroying Tpm-binding ability increased the number of shaft synapses and thin spine motility. Eliminating the actin-binding abilities of Tmod2 increased the number of mushroom spines. Tpm-mediated pointed-end binding decreased F-actin depolymerization, which may positively affect spine stabilization; the nucleation ability of Tmod2 appeared to increase shaft synapses.
    MeSH term(s) Actins ; Dendritic Spines ; Tropomodulin ; Actin Cytoskeleton ; Cytoskeleton
    Chemical Substances Actins ; Tropomodulin
    Language English
    Publishing date 2023-08-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom13081237
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