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  1. Article: Tetraspanins from

    Ruangsuwast, Apisit / Smout, Michael J / Brindley, Paul J / Loukas, Alex / Laha, Thewarach / Chaiyadet, Sujittra

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The liver ... ...

    Abstract The liver fluke
    Language English
    Publishing date 2023-06-12
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.06.12.544604
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Recombinant protein production and functional analysis of a M60-like-2 metallopeptidase enzyme from the carcinogenic liver fluke Opisthorchis viverrini.

    Jumpajan, Jiraporn / Chaiyadet, Sujittra / Saichua, Prasert / Tangkawatana, Sirikachorn / Talabnin, Krajang / Laha, Thewarach / Suttiprapa, Sutas

    Protein expression and purification

    2024  Volume 216, Page(s) 106429

    Abstract: Mucin plays a crucial role in safeguarding mucosal tissues by obstructing the translocation of microorganisms. Mucosal tissue-dwelling parasites must devise a strategy to surmount this mucin barrier in order to establish colonization. In a recent ... ...

    Abstract Mucin plays a crucial role in safeguarding mucosal tissues by obstructing the translocation of microorganisms. Mucosal tissue-dwelling parasites must devise a strategy to surmount this mucin barrier in order to establish colonization. In a recent discovery, it was observed that the liver fluke Opisthorchis viverrini secretes two mucinases, namely Ov-M60-like-1 and Ov-M60-like-2. Ov-M60-like-1 was previously characterized. Here, we study the Ov-M60-like-2 by utilizing the wheat germ expression system to produce recombinant proteins and conducted a functional analysis of its enzymatic activity on bovine submaxillary mucin (BSM). Subsequently, we delved deeper into understanding the role of this enzyme in host-parasite interactions by evaluating its mucinase activity on mucins from the bile duct of O. viverrini-infected hamsters. Through successful production of recombinant proteins using the wheat germ expression system, we observed that this enzyme displayed mucinase activity over a wide pH range (pH 2 to pH 10) against BSM. Our investigations revealed it ability to digest mucin from the bile duct. These findings suggest that Ov-M60-like-2 possess a mucinase activity, together with Ov-M60-like-1, enabling the liver fluke to successful colonization of the host's bile duct.
    MeSH term(s) Cricetinae ; Animals ; Cattle ; Opisthorchis/genetics ; Opisthorchis/chemistry ; Fasciola hepatica ; Carcinogens ; Recombinant Proteins/chemistry ; Metalloproteases ; Mucins
    Chemical Substances Carcinogens ; Recombinant Proteins ; Metalloproteases (EC 3.4.-) ; Mucins
    Language English
    Publishing date 2024-01-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1055455-5
    ISSN 1096-0279 ; 1046-5928
    ISSN (online) 1096-0279
    ISSN 1046-5928
    DOI 10.1016/j.pep.2024.106429
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3084: Show issues Location:
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  3. Article ; Online: Opisthorchis viverrini excretory-secretory products suppress GLUT8 of cholangiocytes.

    Kha, Sandy / Chaiyadet, Sujittra / Saichua, Prasert / Tangkawatana, Sirikachorn / Sripa, Banchob / Suttiprapa, Sutas

    Parasitology research

    2024  Volume 123, Issue 3, Page(s) 161

    Abstract: Opisthorchis viverrini infection and the subsequent bile duct cancer it induces remains a significant public health problem in Southeast Asia. Opisthorchiasis has been reported to cause reduced plasma glucose levels among infected patients. The ... ...

    Abstract Opisthorchis viverrini infection and the subsequent bile duct cancer it induces remains a significant public health problem in Southeast Asia. Opisthorchiasis has been reported to cause reduced plasma glucose levels among infected patients. The underlying mechanism for this phenomenon is unclear. In the present study, evidence is presented to support the hypothesis that O. viverrini exploits host cholangiocyte glucose transporters (GLUTs) in a similar manner to that of rodent intestinal nematodes, to feed on unabsorbed glucose in the bile for survival. GLUT levels in a cholangiocyte H69 cell line co-cultured with excretory-secretory products of O. viverrini were examined using qPCR and immunoblotting. GLUT 8 mRNA and expressed proteins were found to be downregulated in H69 cells in the presence of O. viverrini. This suggests that O. viverrini alters glucose metabolism in cells within its vicinity by limiting transporter expression resulting in increased bile glucose that it can utilize and potentially explains the previously reported anti-insulin effect of opisthorchiasis.
    MeSH term(s) Animals ; Humans ; Bile Duct Neoplasms/metabolism ; Bile Ducts, Intrahepatic ; Glucose/metabolism ; Opisthorchiasis/complications ; Opisthorchiasis/metabolism ; Opisthorchis/metabolism ; Antigens, Helminth/metabolism ; Glucose Transport Proteins, Facilitative/metabolism
    Chemical Substances Glucose (IY9XDZ35W2) ; Antigens, Helminth ; Glucose Transport Proteins, Facilitative
    Language English
    Publishing date 2024-03-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-024-08184-3
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    Uh II Zs.124: Show issues Location:
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  4. Article ; Online: T helper cell responses to Opisthorchis viverrini infection associate with host susceptibility.

    Srisai, Pattaraporn / Chaiyadet, Sujittra / Jumnainsong, Amonrat / Suttiprapa, Sutas / Leelayuwat, Chanvit / Saichua, Prasert

    Parasitology research

    2024  Volume 123, Issue 2, Page(s) 135

    Abstract: Opisthorchis viverrini infection is endemic in the lower Mekong subregion. The liver is an organ that worms are drawn to and cause damage. However, the immune-related susceptibility in the liver is poorly understood. In this study, we investigated T ... ...

    Abstract Opisthorchis viverrini infection is endemic in the lower Mekong subregion. The liver is an organ that worms are drawn to and cause damage. However, the immune-related susceptibility in the liver is poorly understood. In this study, we investigated T helper (Th) cell responses in the liver of BALB/c mice and golden Syrian hamsters during 2-28 days post-infection (DPI). We found that Th cell responses were distinct between mice and hamsters in terms of dynamics and polarization. Mice exhibited the early induction of Th1, Th2, Th17, and regulatory T (Treg) cells responses after the presence of O. viverrini worms at 2 DPI. In hamsters, the late induction of Th1/Th17, downregulation of Th2/Treg responses and early elevation of suppressive cytokine interleukin (IL)-10 were found together with swift reduction of Th cell numbers. Interestingly, expressions of IL-4 (Th2 functional cytokine) and Foxp3 (Treg lineage) were completely different between mice and hamsters which elevated in mice but suppressed in hamsters. These results suggest that early induction and well-regulation are related to host resistance. In contrast, late induction of Th cell response might allow immature worms to develop in the host. Our findings provide a greater understanding in Th cell response-related susceptibility in O. viverrini infection which would be targeting immunity for the development of immune-based intervention such as vaccine.
    MeSH term(s) Cricetinae ; Animals ; Mice ; Opisthorchiasis/prevention & control ; Opisthorchis ; Mesocricetus ; Cytokines ; T-Lymphocytes, Helper-Inducer/metabolism
    Chemical Substances Cytokines
    Language English
    Publishing date 2024-02-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-024-08154-9
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  5. Article ; Online: Tetraspanins from the liver fluke Opisthorchis viverrini stimulate cholangiocyte migration and inflammatory cytokine production.

    Ruangsuwast, Apisit / Smout, Michael J / Brindley, Paul J / Loukas, Alex / Laha, Thewarach / Chaiyadet, Sujittra

    Folia parasitologica

    2023  Volume 70

    Abstract: The liver fluke Opisthorchis viverrini (Poirier, 1886) (Digenea) secretes extracellular vesicles (EVs) bearing CD63-like tetraspanins on their surface. Fluke EVs are actively internalised by host cholangiocytes in the bile ducts, where they drive ... ...

    Abstract The liver fluke Opisthorchis viverrini (Poirier, 1886) (Digenea) secretes extracellular vesicles (EVs) bearing CD63-like tetraspanins on their surface. Fluke EVs are actively internalised by host cholangiocytes in the bile ducts, where they drive pathology and promote neoplasia through induction of cellular proliferation and secretion of inflammatory cytokines. We investigated the effects of tetraspanins of the CD63 superfamily by co-culturing recombinant forms of the large extracellular loop (LEL) of O. viverrini tetraspanin-2 (rLEL-Ov-TSP-2) and tetraspanin-3 (rLEL-Ov-TSP-3) with non-cancerous human bile duct (H69) and cholangiocarcinoma (CCA, M213) cell lines. The results showed that cell lines co-cultured with excretory/secretory products from adult O. viverrini (Ov-ES) underwent significantly increased cell proliferation at 48 hours but not 24 hours compared to untreated control cells (P < 0.05), whereas rLEL-Ov-TSP-3 co-culture resulted in significantly increased cell proliferation at both 24 hours (P < 0.05) and 48 hours (P < 0.01) time points. In like fashion, H69 cholangiocytes co-cultured with both Ov-ES and rLEL-Ov-TSP-3 underwent significantly elevated Il-6 and Il-8 gene expression for at least one of the time points assessed. Finally, both rLEL-Ov-TSP-2 and rLEL-Ov-TSP-3 significantly enhanced migration of both M213 and H69 cell lines. These findings indicated that O. viverrini CD63 family tetraspanins can promote a cancerous microenvironment by enhancing innate immune responses and migration of biliary epithelial cells.
    MeSH term(s) Adult ; Humans ; Animals ; Fasciola hepatica ; Opisthorchis ; Epithelial Cells ; Cell Line ; Cytokines
    Chemical Substances Cytokines
    Language English
    Publishing date 2023-09-16
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 162048-4
    ISSN 1803-6465 ; 0015-5683
    ISSN (online) 1803-6465
    ISSN 0015-5683
    DOI 10.14411/fp.2023.017
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 212: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  6. Article: Peptide derived from progranulin of the carcinogenic liver fluke, Opisthorchis viverrini stimulates cell hyperproliferation and proinflammatory cytokine production.

    Hembasat, Thanapat / Chaiyadet, Sujittra / Ittiprasert, Wannaporn / Smout, Michael J / Young, Neil D / Loukas, Alex / Brindley, Paul J / Laha, Thewarach

    Research square

    2023  

    Abstract: Purpose: Progranulin (PGRN) is a secreted glycoprotein growth factor with roles in wound healing, inflammation, angiogenesis and malignancy. An orthologue of the gene encoding human PGRN was identified in the carcinogenic liver fluke : Methods: ... ...

    Abstract Purpose: Progranulin (PGRN) is a secreted glycoprotein growth factor with roles in wound healing, inflammation, angiogenesis and malignancy. An orthologue of the gene encoding human PGRN was identified in the carcinogenic liver fluke
    Methods: Sequence structure, general characteristics and possible function of
    Results: The structure of the gene coding for
    Conclusion: Ov-PGRN is expressed throughout the life cycle of liver fluke, and likely plays a key role in development and growth.
    Language English
    Publishing date 2023-03-14
    Publishing country United States
    Document type Preprint
    DOI 10.21203/rs.3.rs-2586058/v1
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  7. Article: Small extracellular vesicles but not microvesicles from

    Chaiyadet, Sujittra / Sotillo, Javier / Smout, Michael / Cooper, Martha / Doolan, Denise L / Waardenberg, Ashley / Eichenberger, Ramon M / Field, Matt / Brindley, Paul J / Laha, Thewarach / Loukas, Alex

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Chronic infection ... ...

    Abstract Chronic infection with
    Language English
    Publishing date 2023-05-22
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.22.540805
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Monoclonal Antibodies Targeting an

    Phumrattanaprapin, Wuttipong / Pearson, Mark / Pickering, Darren / Tedla, Bemnet / Smout, Michael / Chaiyadet, Sujittra / Brindley, Paul J / Loukas, Alex / Laha, Thewarach

    Vaccines

    2021  Volume 9, Issue 7

    Abstract: Opisthorchis ... ...

    Abstract Opisthorchis viverrini
    Language English
    Publishing date 2021-07-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9070740
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  9. Article ; Online: Silencing of

    Chaiyadet, Sujittra / Sotillo, Javier / Krueajampa, Watchara / Thongsen, Sophita / Smout, Michael / Brindley, Paul J / Laha, Thewarach / Loukas, Alex

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 827521

    Abstract: Inter-phylum transfer of molecular information is exquisitely exemplified in the uptake of parasite extracellular vesicles (EVs) by their target mammalian host tissues. The oriental liver fluke, ...

    Abstract Inter-phylum transfer of molecular information is exquisitely exemplified in the uptake of parasite extracellular vesicles (EVs) by their target mammalian host tissues. The oriental liver fluke,
    MeSH term(s) Animals ; Extracellular Vesicles ; Gene Expression ; Humans ; Mammals/genetics ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Opisthorchis/genetics ; Opisthorchis/metabolism ; Tetraspanins/genetics
    Chemical Substances MicroRNAs ; Tetraspanins
    Language English
    Publishing date 2022-02-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.827521
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  10. Article ; Online: Monoclonal Antibodies Targeting an Opisthorchis viverrini Extracellular Vesicle Tetraspanin Protect Hamsters against Challenge Infection

    Wuttipong Phumrattanaprapin / Mark Pearson / Darren Pickering / Bemnet Tedla / Michael Smout / Sujittra Chaiyadet / Paul J. Brindley / Alex Loukas / Thewarach Laha

    Vaccines, Vol 9, Iss 740, p

    2021  Volume 740

    Abstract: Opisthorchis viverrini causes severe pathology in the bile ducts of infected human hosts, and chronic infection can culminate in bile duct cancer. The prevention of infection by vaccination would decrease opisthorchiasis-induced morbidity and mortality. ... ...

    Abstract Opisthorchis viverrini causes severe pathology in the bile ducts of infected human hosts, and chronic infection can culminate in bile duct cancer. The prevention of infection by vaccination would decrease opisthorchiasis-induced morbidity and mortality. The tetraspanin protein, Ov -TSP-2, is located on the membrane of secreted extracellular vesicles (EVs), and is a candidate antigen for inclusion in a subunit vaccine. To address the role of anti- Ov -TSP-2 antibodies in protection, we assessed the protective capacity of anti- Ov -TSP-2 monoclonal antibodies (mAbs) against opisthorchiasis. Two anti-TSP-2 IgM mAbs, 1D6 and 3F5, and an isotype control were passively transferred to hamsters, followed by parasite challenge one day later. Hamsters that received 3F5 had 74.5% fewer adult flukes and 67.4% fewer eggs per gram of feces compared to hamsters that received the control IgM. Both 1D6 and 3F5 (but not the control IgM) blocked the uptake of fluke EVs by human bile duct epithelial cells in vitro. This is the first report of passive immunization against human liver fluke infection, and the findings portend the feasibility of antibody-directed therapies for liver fluke infection, bolstering the selection of TSPs as components of a subunit vaccine for opisthorchiasis and fluke infections generally.
    Keywords passive immunization ; monoclonal antibody ; Opisthorchis viverrini ; tetraspanin ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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