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  1. Article: Physical Activity, Body Composition, Physical Fitness, and Body Dissatisfaction in Physical Education of Extremadura Adolescents: An Exploratory Study.

    Moreno-Díaz, María Isabel / Vaquero-Solís, Miguel / Tapia-Serrano, Miguel Ángel / Sánchez-Miguel, Pedro Antonio

    Children (Basel, Switzerland)

    2024  Volume 11, Issue 1

    Abstract: In recent years, physical activity levels among youths have declined significantly. This has led to a decline in adherence to physical activity recommendations. In this sense, physical education offers an ideal environment that contributes positively to ... ...

    Abstract In recent years, physical activity levels among youths have declined significantly. This has led to a decline in adherence to physical activity recommendations. In this sense, physical education offers an ideal environment that contributes positively to improving adherence to physical activity recommendations, as it teaches students movement-related skills and knowledge. The objective of the present research was to investigate the relationship between physical activity levels, body composition, fitness in Physical Education, and body dissatisfaction levels, and to analyse sex differences in relation to the study variables. The sample was formed of 1166 participants from the 1st and 2nd secondary compulsory education, of which 642 were boys (age 13.16 ± 0.91), and 524 girls (age 13.08 ± 0.85). The measure of physical activity was the Physical Activity Questionnaire for Adolescents (PAQ-A). Body composition was assessed using weight and height to calculate their body mass index. Cardiorespiratory capacity was assessed using the Course-Navette test in Physical Education lessons. The results showed the relationship between physical activity and body mass index, cardiorespiratory capacity, and body satisfaction. It was also confirmed that higher levels of physical activity were associated with a lower body mass index, improvements in cardiorespiratory fitness, and lower levels of body dissatisfaction to a greater extent in boys than in girls. The study concludes that improvements in the study variables were associated with increased physical activity. In addition, it seems necessary to promote healthy lifestyles in physical education lessons, especially during adolescence, as they could serve as a gateway for the improvement of health-related fitness in future generations. Increasing the amount of physical activity among young people is vital. Therefore, it would be essential to develop intervention programs in physical education classes, especially during adolescence, aimed at promoting and increasing physical activity and its benefits.
    Language English
    Publishing date 2024-01-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2732685-8
    ISSN 2227-9067
    ISSN 2227-9067
    DOI 10.3390/children11010083
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Nested PCR Telomere Fusion Assay Highlights the Widespread End-Capping Protection of Arabidopsis CTC1.

    Vaquero-Sedas, María I / Vega-Palas, Miguel A

    International journal of molecular sciences

    2024  Volume 25, Issue 1

    Abstract: Telomeres protect the ends of linear eukaryotic chromosomes from being recognized as DNA double-strand breaks. Two major protein complexes are involved in the protection of telomeres: shelterin and CST. The dysfunction of these complexes can challenge ... ...

    Abstract Telomeres protect the ends of linear eukaryotic chromosomes from being recognized as DNA double-strand breaks. Two major protein complexes are involved in the protection of telomeres: shelterin and CST. The dysfunction of these complexes can challenge the function of telomeres and lead to telomere fusions, breakage-fusion-bridge cycles, and cell death. Therefore, monitoring telomere fusions helps to understand telomeres biology. Telomere fusions are often analyzed by Fluorescent In Situ Hybridization (FISH) or PCR. Usually, both methods involve hybridization with a telomeric probe, which allows the detection of fusions containing telomeric sequences, but not of those lacking them. With the aim of detecting both types of fusion events, we have developed a nested PCR method to analyze telomere fusions in
    MeSH term(s) Arabidopsis/genetics ; In Situ Hybridization, Fluorescence ; Polymerase Chain Reaction ; Shelterin Complex ; Telomere/genetics ; Telomere-Binding Proteins/genetics ; Arabidopsis Proteins/genetics
    Chemical Substances Shelterin Complex ; CTC1 protein, Arabidopsis ; Telomere-Binding Proteins ; Arabidopsis Proteins
    Language English
    Publishing date 2024-01-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25010672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A Nested PCR Telomere Fusion Assay Highlights the Widespread End-Capping Protection of Arabidopsis CTC1

    María I. Vaquero-Sedas / Miguel A. Vega-Palas

    International Journal of Molecular Sciences, Vol 25, Iss 1, p

    2024  Volume 672

    Abstract: Telomeres protect the ends of linear eukaryotic chromosomes from being recognized as DNA double-strand breaks. Two major protein complexes are involved in the protection of telomeres: shelterin and CST. The dysfunction of these complexes can challenge ... ...

    Abstract Telomeres protect the ends of linear eukaryotic chromosomes from being recognized as DNA double-strand breaks. Two major protein complexes are involved in the protection of telomeres: shelterin and CST. The dysfunction of these complexes can challenge the function of telomeres and lead to telomere fusions, breakage–fusion–bridge cycles, and cell death. Therefore, monitoring telomere fusions helps to understand telomeres biology. Telomere fusions are often analyzed by Fluorescent In Situ Hybridization (FISH) or PCR. Usually, both methods involve hybridization with a telomeric probe, which allows the detection of fusions containing telomeric sequences, but not of those lacking them. With the aim of detecting both types of fusion events, we have developed a nested PCR method to analyze telomere fusions in Arabidopsis thaliana . This method is simple, accurate, and does not require hybridization. We have used it to analyze telomere fusions in wild-type and mutant plants altered in CTC1, one of the three components of the Arabidopsis CST telomere capping complex. Our results show that null ctc1-2 mutant plants display fusions between all telomeric regions present in Arabidopsis chromosomes 1, 3 and 5, thus highlighting the widespread end-capping protection achieved by CTC1.
    Keywords Arabidopsis thaliana ; telomere capping ; telomere fusions analysis ; genome stability ; CST complex ; CTC1 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Sedentary behaviours and their relationship with academic performance in adolescents: A mediation analysis.

    Sánchez-Miguel, Pedro Antonio / Molina-López, Jorge / Vaquero-Solís, Miguel / Tapia-Serrano, Miguel Angel

    Journal of sports sciences

    2023  Volume 40, Issue 23, Page(s) 2570–2577

    Abstract: The present research aims to examine the relationship between the four dimensions of sedentary behaviours and academic performance, as well as to test the association between sedentary behaviours and academic performance in boys and girls in a sample of ... ...

    Abstract The present research aims to examine the relationship between the four dimensions of sedentary behaviours and academic performance, as well as to test the association between sedentary behaviours and academic performance in boys and girls in a sample of Spanish adolescents. A total of 2,216 adolescents aged 11 to 16 years old, both males (13.30 ± 0.92) and females (13.06 ± 0.86), participated. Sleep duration, physical activity, and sedentary behaviours were measured through questionnaires. Academic performance was assessed with students´ marks. Potential confounders such as sex, age, socioeconomic status, and cardiorespiratory fitness were also measured. Descriptive analysis, correlation analysis, analysis of differences, and multiple mediation analysis were conducted. It was observed that girls spend more time on sedentary educational or social behaviours, while boys spend much more time on screen time. Sedentary behaviours such as educational time, screen time, and relaxing time were associated with academic performance. Specifically, educational time appears to be positively related to academic performance. Nevertheless, screen time and relaxing time were negatively associated with academic performance. The multiple mediation analysis revealed that screen time was the most negative behaviour on academic performance. The outcomes are discussed to improve knowledge about sedentary behaviours and academic performance.
    MeSH term(s) Male ; Female ; Humans ; Adolescent ; Child ; Sedentary Behavior ; Mediation Analysis ; Academic Performance ; Exercise ; Educational Status
    Language English
    Publishing date 2023-02-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 995528-8
    ISSN 1466-447X ; 0264-0414
    ISSN (online) 1466-447X
    ISSN 0264-0414
    DOI 10.1080/02640414.2023.2174731
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Intervention Programme Based on Self-Determination Theory to Promote Extracurricular Physical Activity through Physical Education in Primary School: A Study Protocol.

    Llanos-Muñoz, Rubén / Vaquero-Solís, Mikel / López-Gajardo, Miguel Ángel / Sánchez-Miguel, Pedro Antonio / Tapia-Serrano, Miguel Ángel / Leo, Francisco Miguel

    Children (Basel, Switzerland)

    2023  Volume 10, Issue 3

    Abstract: Low levels of physical activity (PA) are a concern among students, producing negative physical, health and mental consequences. This study aims to present a protocol intervention in physical education (PE) based on self-determination theory (SDT) to ... ...

    Abstract Low levels of physical activity (PA) are a concern among students, producing negative physical, health and mental consequences. This study aims to present a protocol intervention in physical education (PE) based on self-determination theory (SDT) to enhance students' motivation towards performing PA and increasing their PA levels in their leisure time. This protocol is a convenience study with two allocation arms (intervention group versus control group). SDT-based motivational strategies will be carried out and co-created with PE teachers to increase motivation and out-of-school PA levels. Data collection will be conducted three times: before the intervention, after the intervention (four months after baseline) and at the end of the intervention (retention measurement, seven months after baseline). The measures will assess perceived teacher support for PA, motivation towards PA, intention to be physically active, PA levels, engagement in PE and academic performance. Overall, this intervention programme is expected to increase students' autonomous motivation for PA and their PA levels in their free time. This intervention might encourage teachers to establish strategies and resources to increase their students' adaptive outcomes.
    Language English
    Publishing date 2023-03-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2732685-8
    ISSN 2227-9067
    ISSN 2227-9067
    DOI 10.3390/children10030504
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: The Mediating Role of Motivational Regulation on the Relationship of Emotional Intelligence with Physical Activity in Spanish Schoolchildren.

    Vaquero-Solís, Mikel / Tapia-Serrano, Miguel Angel / Sánchez-Miguel, Pedro Antonio

    Children (Basel, Switzerland)

    2022  Volume 9, Issue 11

    Abstract: The present study aimed to analyze the mediating role of different types of motivational regulations in the relationship established between emotional intelligence and physical activity. Participants were 431 secondary school students aged 12 to 16 years, ...

    Abstract The present study aimed to analyze the mediating role of different types of motivational regulations in the relationship established between emotional intelligence and physical activity. Participants were 431 secondary school students aged 12 to 16 years, 51.5% females (13.59 ± 1.03 years) and 48.5% males (13.50 ± 0.94 years), who completed a self-reported questionnaire of emotional intelligence, motivation and physical activity. The results showed a statistically significant positive association between emotional intelligence, physical activity, and more self-determined forms of motivation (intrinsic regulation, identified regulation and introjected regulation) (p < 0.05). Several mediation models were also presented that confirmed the mediating value of the more self-determined motivational regulations in the association established between emotional intelligence and physical activity, with the indirect effects being significant for intrinsic regulation, identified regulation, and introjected regulation (p < 0.05). Finally, we conclude on the importance of the management of emotions in order to propitiate a suitable motivational state that leads to physical activity. Therefore, this study highlights the importance of emotional intelligence for the practice of different forms of physical activity in young people.
    Language English
    Publishing date 2022-10-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2732685-8
    ISSN 2227-9067
    ISSN 2227-9067
    DOI 10.3390/children9111656
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Epigenetic nature of Arabidopsis thaliana telomeres.

    Vaquero-Sedas, María I / Vega-Palas, Miguel A

    Plant physiology

    2022  Volume 191, Issue 1, Page(s) 47–55

    Abstract: The epigenetic features of defined chromosomal domains condition their biochemical and functional properties. Therefore, there is considerable interest in studying the epigenetic marks present at relevant chromosomal loci. Telomeric regions, which ... ...

    Abstract The epigenetic features of defined chromosomal domains condition their biochemical and functional properties. Therefore, there is considerable interest in studying the epigenetic marks present at relevant chromosomal loci. Telomeric regions, which include telomeres and subtelomeres, have been traditionally considered heterochromatic. However, whereas the heterochromatic nature of subtelomeres has been widely accepted, the epigenetic status of telomeres remains controversial. Here, we studied the epigenetic features of Arabidopsis (Arabidopsis thaliana) telomeres by analyzing multiple genome-wide ChIP-seq experiments. Our analyses revealed that Arabidopsis telomeres are not significantly enriched either in euchromatic marks like H3K4me2, H3K9ac, and H3K27me3 or in heterochromatic marks such as H3K27me1 and H3K9me2. Thus, telomeric regions in Arabidopsis have a bimodal chromatin organization with telomeres lacking significant levels of canonical euchromatic and heterochromatic marks followed by heterochromatic subtelomeres. Since heterochromatin is known to influence telomere function, the heterochromatic modifications present at Arabidopsis subtelomeres could play a relevant role in telomere biology.
    MeSH term(s) Arabidopsis/genetics ; Epigenesis, Genetic ; Telomere/genetics ; Heterochromatin/genetics
    Chemical Substances Heterochromatin
    Language English
    Publishing date 2022-10-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiac471
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Next generation in ultrasound imaging to assess upper airway.

    Fernandez-Vaquero, Miguel Ángel / Delgado-Cidranes, Ernesto / Greif, Robert

    Brazilian journal of anesthesiology (Elsevier)

    2022  Volume 72, Issue 5, Page(s) 673–675

    MeSH term(s) Airway Management/methods ; Humans ; Intubation, Intratracheal/methods ; Laryngoscopy/methods ; Larynx ; Trachea ; Ultrasonography/methods
    Language English
    Publishing date 2022-06-15
    Publishing country Brazil
    Document type Journal Article
    ISSN 2352-2291
    ISSN (online) 2352-2291
    DOI 10.1016/j.bjane.2022.05.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Response to the letter to the editor: A comment on C-reactive protein and SOFA scale: a simple scale as an early predictor of the need for critical care in patients with COVID-19 pneumonia in Spain.

    Vaquero Roncero, Luis Mario / Sánchez Barrado, Elisa / Sánchez Hernández, Miguel Vicente

    Revista espanola de anestesiologia y reanimacion

    2022  Volume 69, Issue 7, Page(s) 442–443

    MeSH term(s) C-Reactive Protein/analysis ; COVID-19 ; Critical Care ; Humans ; SARS-CoV-2 ; Spain/epidemiology
    Chemical Substances C-Reactive Protein (9007-41-4)
    Language English
    Publishing date 2022-07-20
    Publishing country Spain
    Document type Letter ; Comment
    ISSN 2341-1929
    ISSN (online) 2341-1929
    DOI 10.1016/j.redare.2022.07.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Pattern of Consumption of Sports Supplements of Spanish Handball Players: Differences According to Gender and Competitive Level.

    Romero-García, David / Martínez-Sanz, José Miguel / Sebastiá-Rico, Jaime / Manchado, Carmen / Vaquero-Cristóbal, Raquel

    Nutrients

    2024  Volume 16, Issue 2

    Abstract: 1) Background: Given the physiological characteristics of handball, players may require the use of certain sports supplements (SS). However, very few studies have investigated the consumption of SS in handball. The aims were to determine the number of ... ...

    Abstract (1) Background: Given the physiological characteristics of handball, players may require the use of certain sports supplements (SS). However, very few studies have investigated the consumption of SS in handball. The aims were to determine the number of handball players who consume SS, to analyze their SS consumption pattern according to gender and competitive level, and to assess whether the SS they consume are supported by scientific evidence, and to which group they belong according to the classification of the Australian Institute of Sport (AIS). (2) Methods: A descriptive-correlational study was carried out on the habitual consumption of SS in 360 federated Spanish players by using a self-administered and validated questionnaire. (3) Results: These showed 65.8% of the sample consumed SS. According to the total number of participants, the most consumed supplements were from Group A: sports drinks (30.8%) and whey protein (30.4%). When analyzing the data by gender, the men's consumption was significantly higher for whey protein (
    MeSH term(s) Male ; Humans ; Creatine ; Whey Proteins ; Australia ; Sports/physiology ; Athletes
    Chemical Substances Creatine (MU72812GK0) ; Whey Proteins
    Language English
    Publishing date 2024-01-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu16020315
    Database MEDical Literature Analysis and Retrieval System OnLINE

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