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Article: Improving NonViral Gene Delivery Using MHz Bursts of Nanosecond Pulses and Gold Nanoparticles for Electric Field Amplification.

Radzevičiūtė-Valčiukė, Eivina / Gečaitė, Jovita / Želvys, Augustinas / Zinkevičienė, Auksė / Žalnėravičius, Rokas / Malyško-Ptašinskė, Veronika / Nemeikaitė-Čenienė, Aušra / Kašėta, Vytautas / German, Natalija / Novickij, Jurij / Ramanavičienė, Almira / Kulbacka, Julita / Novickij, Vitalij

Pharmaceutics

2023 Volume 15, Issue 4

Abstract: Gene delivery by the pulsed electric field is a promising alternative technology for nonviral transfection; however, the application of short pulses (i.e., nanosecond) is extremely limited. In this work, we aimed to show the capability to improve gene ... ...

Abstract	Gene delivery by the pulsed electric field is a promising alternative technology for nonviral transfection; however, the application of short pulses (i.e., nanosecond) is extremely limited. In this work, we aimed to show the capability to improve gene delivery using MHz frequency bursts of nanosecond pulses and characterize the potential use of gold nanoparticles (AuNPs: 9, 13, 14, and 22 nm) in this context. We have used bursts of MHz pulses 3/5/7 kV/cm × 300 ns × 100 and compared the efficacy of the parametric protocols to conventional microsecond protocols (100 µs × 8, 1 Hz) separately and in combination with nanoparticles. Furthermore, the effects of pulses and AuNPs on the generation of reactive oxygen species (ROS) were analyzed. It was shown that gene delivery using microsecond protocols could be significantly improved with AuNPs; however, the efficacy is strongly dependent on the surface charge of AuNPs and their size. The capability of local field amplification using AuNPs was also confirmed by finite element method simulation. Finally, it was shown that AuNPs are not effective with nanosecond protocols. However, MHz protocols are still competitive in the context of gene delivery, resulting in low ROS generation, preserved viability, and easier procedure to trigger comparable efficacy.
Language	English
Publishing date	2023-04-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics15041178
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Article ; Online: Extracellular Vesicles from Human Teeth Stem Cells Trigger ATP Release and Promote Migration of Human Microglia through P2X4 Receptor/MFG-E8-Dependent Mechanisms.

Jonavičė, Ugnė / Romenskaja, Diana / Kriaučiūnaitė, Karolina / Jarmalavičiūtė, Akvilė / Pajarskienė, Justina / Kašėta, Vytautas / Tunaitis, Virginijus / Malm, Tarja / Giniatulin, Rashid / Pivoriūnas, Augustas

International journal of molecular sciences

2021 Volume 22, Issue 20

Abstract: Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate the neuroinflammatory response of microglia remains largely unexplored. Here, ...

Abstract	Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate the neuroinflammatory response of microglia remains largely unexplored. Here, we addressed this issue by testing the action of EVs derived from human exfoliated deciduous teeth stem cells (SHEDs) on immortalized human microglial cells. We found that EVs induced a rapid increase in intracellular Ca
MeSH term(s)	Adenosine Triphosphate/metabolism ; Antigens, Surface/metabolism ; Calcium/metabolism ; Cell Movement ; Cells, Cultured ; Dental Pulp/cytology ; Extracellular Vesicles/metabolism ; Extracellular Vesicles/transplantation ; Humans ; Microglia/cytology ; Microglia/metabolism ; Milk Proteins/metabolism ; Receptors, Purinergic P2X4/metabolism ; Stem Cells/cytology ; Stem Cells/metabolism
Chemical Substances	Antigens, Surface ; MFGE8 protein, human ; Milk Proteins ; Receptors, Purinergic P2X4 ; Adenosine Triphosphate (8L70Q75FXE) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2021-10-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms222010970
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Article ; Online: Tooth Loss and Nutritional Status in Older Adults: A Systematic Review and Meta-analysis.

Zelig, R / Goldstein, S / Touger-Decker, R / Firestone, E / Golden, A / Johnson, Z / Kaseta, A / Sackey, J / Tomesko, J / Parrott, J S

JDR clinical and translational research

2020 Volume 7, Issue 1, Page(s) 4–15

Abstract: Background/objective: Older adults are at risk for tooth loss and compromised nutritional status. Our objective was to conduct a systematic review and meta-analysis to answer the following question: Among adults aged ≥60 y living in developed countries, ...

Abstract	Background/objective: Older adults are at risk for tooth loss and compromised nutritional status. Our objective was to conduct a systematic review and meta-analysis to answer the following question: Among adults aged ≥60 y living in developed countries, what are the associations between tooth loss and nutritional status as assessed by a validated nutrition screening or assessment tool? Methods: PRISMA guidelines were followed. PubMed, Scopus, CINAHL, Web of Science, and MEDLINE were searched for studies published in English between 2009 and 2019 that met inclusion criteria. Data extracted included study and participant characteristics, dentition, and nutritional status. Risk of bias was assessed with a modified Newcastle-Ottawa Scale. Random effects meta-analysis was used. Results: Of the 588 unduplicated articles identified, 78 were reviewed in full text, and 7 met inclusion criteria. Six studies were combined for a meta-analysis, which revealed that individuals who were completely edentulous or who lacked functional dentition had a 21% increased likelihood of being at risk of malnutrition or being malnourished, as compared with those who were dentulous or had functionally adequate dentition (risk ratio, 1.21; 95% CI, 1.11 to 1.32; I Conclusions and implications: Findings suggest that older adults with tooth loss are at greater risk of malnutrition than those with functionally adequate dentition. Use of validated tools to assess risk of malnutrition in older adults with tooth loss is important to promote early intervention and referral to optimize nutrition and oral health status. Findings were limited by heterogeneity, risk of bias, and overall quality of the studies reviewed. Cohort studies that adjust for known confounders and use consistent approaches to assess tooth loss and nutritional status are needed. Knowledge transfer statement: The results of this study suggest that older adults with tooth loss are at greater risk of malnutrition than those with functionally adequate dentition. Screening of this population for malnutrition by health care professionals, including dentists and dietitians, may result in corresponding referrals to optimize nutrition and oral health status. Further research is needed with consistent approaches to assess tooth loss and nutritional status.
MeSH term(s)	Aged ; Eating ; Humans ; Malnutrition/diagnosis ; Mouth, Edentulous ; Nutritional Status ; Tooth Loss/epidemiology
Language	English
Publishing date	2020-12-21
Publishing country	United States
Document type	Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	2842304-5
ISSN	2380-0852 ; 2380-0844
ISSN (online)	2380-0852
ISSN	2380-0844
DOI	10.1177/2380084420981016
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Article ; Online: Incidence of the remnant femoral attachment of the ruptured ACL.

Wittstein, Jocelyn / Kaseta, Maria / Sullivan, Robert / Garrett, William E

Clinical orthopaedics and related research

2009 Volume 467, Issue 10, Page(s) 2691–2694

Abstract: The presence of remnant tibial and femoral attachments of the ruptured ACL has been described in the literature but the femoral remnant has not been well described as a landmark for tunnel placement during reconstruction. We reviewed operative reports, ... ...

Abstract	The presence of remnant tibial and femoral attachments of the ruptured ACL has been described in the literature but the femoral remnant has not been well described as a landmark for tunnel placement during reconstruction. We reviewed operative reports, pictures, and videotapes from 111 ACL reconstructions to determine the incidence of a remnant femoral stump. Patients were divided into two groups: Group A included patients treated from January 2006 through September 2006 (n = 63) when the presence of the femoral footprint was documented retrospectively and Group B included patients treated from September 2006 through June 2007 (n = 48) when the presence or absence of the femoral footprint was documented prospectively. In Group A, there were 48 of 58 (83%) patients with a visible stump and 10 (17%) patients in whom we could not verify the existence of the stump. In Group B, 43 of 44 (98%) patients had a visible stump on the lateral femoral wall that was adequate as a guide for femoral tunnel placement. The native femoral footprint is seen in most cases of ACL reconstruction and can be used for guidance during femoral tunnel preparation.
MeSH term(s)	Adolescent ; Adult ; Anterior Cruciate Ligament/pathology ; Anterior Cruciate Ligament/surgery ; Anterior Cruciate Ligament Injuries ; Arthroscopy/adverse effects ; Female ; Femur/pathology ; Femur/surgery ; Humans ; Male ; Middle Aged ; Prospective Studies ; Retrospective Studies ; Rupture ; Treatment Outcome ; Young Adult
Language	English
Publishing date	2009-04-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	80301-7
ISSN	1528-1132 ; 0009-921X
ISSN (online)	1528-1132
ISSN	0009-921X
DOI	10.1007/s11999-009-0805-y
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Article: Cardiovascular disease in the diabetic woman.

Kaseta, J R / Skafar, D F / Ram, J L / Jacober, S J / Sowers, J R

The Journal of clinical endocrinology and metabolism

1999 Volume 84, Issue 6, Page(s) 1835–1838

Abstract: The incidence of cardiovascular disease (CVD) with age is increasing in the United States, and elderly women constitute a disproportional component of the aging population. Elderly women also have a relatively high incidence of diabetes, which ... ...

Abstract	The incidence of cardiovascular disease (CVD) with age is increasing in the United States, and elderly women constitute a disproportional component of the aging population. Elderly women also have a relatively high incidence of diabetes, which contributes to this relatively high CVD risk. Although CVD is less common in premenopausal women than in men, this difference begins to disappear after the onset of menopause, presumably related to decreased levels of female sex hormones (estrogen and/or progesterone). Diabetes mellitus removes the normal premenopausal gender-related differences in the prevalence of CVD by mechanisms that are not clearly defined, including metabolic and hemodynamic factors associated with diabetes. Dyslipidemia in diabetes mellitus consists of low high density lipoprotein cholesterol, elevated triglyceride levels, and a small, dense, more atherogenic low density lipoprotein particle (i.e. oxidized). Dyslipidemia interacts with associated hemodynamic (i.e. hypertension) and metabolic abnormalities (i.e. increased platelet aggregation and plasminogen activator inhibitor-1 levels) to promote CVD risks in diabetic women. Recent controlled trials underscore the critical importance of aggressively treating CVD risk factors, especially dyslipidemia, in women with diabetes.
MeSH term(s)	Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/etiology ; Cardiovascular Diseases/pathology ; Cardiovascular Diseases/prevention & control ; Clinical Trials as Topic ; Diabetes Complications ; Diabetes Mellitus/epidemiology ; Diabetes Mellitus/pathology ; Diabetes Mellitus/prevention & control ; Female ; Humans ; Male
Language	English
Publishing date	1999-06
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	3029-6
ISSN	1945-7197 ; 0021-972X
ISSN (online)	1945-7197
ISSN	0021-972X
DOI	10.1210/jcem.84.6.5735
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Article: Effect of CGS 20267 on ovarian aromatase and gonadotropin levels in the rat.

Sinha, S / Kaseta, J / Santner, S J / Demers, L M / Bremmer, W J / Santen, R J

Breast cancer research and treatment

1998 Volume 48, Issue 1, Page(s) 45–51

Abstract: Aromatase catalyzes the rate limiting step that converts androgens to estrogens. Postmenopausal women with hormone dependent breast cancer respond to first generation aromatase inhibitors such as aminoglutethimide with a marked suppression of circulating ...

Abstract	Aromatase catalyzes the rate limiting step that converts androgens to estrogens. Postmenopausal women with hormone dependent breast cancer respond to first generation aromatase inhibitors such as aminoglutethimide with a marked suppression of circulating estradiol levels. In contrast, premenopausal women appear to be resistant to first generation aromatase inhibitors. The inability to block ovarian aromatase results from the low affinity of first generation inhibitors for the active site of the enzyme. Under these circumstances, the high substrate levels in the premenopausal ovary compete effectively with these inhibitors and do not allow binding of inhibitor to the active site of the enzyme. Second and third generation aromatase inhibitors with higher affinity for aromatase have now been developed and potentially could block ovarian aromatase. To test this possibility, we administered CGS 20267 (letrozole), a highly potent aromatase inhibitor, to cycling female rats. A dose dependent inhibition of uterine weight occurred with maximum effects produced by the 5 mg/kg/day dosage. During a period of 4 weeks, uterine weight was reduced to levels induced by ovariectomy. Ovarian tissue estradiol levels were inhibited by approximately 80%. As a reflection of inhibition of ovarian aromatase activity, the levels of androstenedione in the ovary increased by an order of magnitude. Both LH and FSH plasma levels increased but not to those observed after ovariectomy. The rise in gonadotropin levels induced a statistically significant but relatively small increase in ovarian weights. These results demonstrate the ability to persistently block ovarian aromatase activity in cycling rats with a potent aromatase inhibitor. This study provides a rationale for clinical trials of potent aromatase inhibitors in pre-menopausal women with breast cancer.
MeSH term(s)	Animals ; Antineoplastic Agents/pharmacology ; Aromatase Inhibitors ; Enzyme Inhibitors/pharmacology ; Female ; Gonadotropins, Pituitary/blood ; Letrozole ; Nitriles/pharmacology ; Organ Size/drug effects ; Ovary/drug effects ; Ovary/metabolism ; Rats ; Rats, Sprague-Dawley ; Triazoles/pharmacology
Chemical Substances	Antineoplastic Agents ; Aromatase Inhibitors ; Enzyme Inhibitors ; Gonadotropins, Pituitary ; Nitriles ; Triazoles ; Letrozole (7LKK855W8I)
Language	English
Publishing date	1998-03
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article
ZDB-ID	604563-7
ISSN	1573-7217 ; 0167-6806
ISSN (online)	1573-7217
ISSN	0167-6806
DOI	10.1023/a:1005937900788
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Article: Aromatase activity and expression in breast cancer and benign breast tissue stromal cells.

Santner, S J / Pauley, R J / Tait, L / Kaseta, J / Santen, R J

The Journal of clinical endocrinology and metabolism

1997 Volume 82, Issue 1, Page(s) 200–208

Abstract: In situ estrogen synthesis by hormone-dependent breast cancers could potentially regulate cellular proliferation through autocrine or paracrine mechanisms. Several biochemical studies have demonstrated activity of the enzyme aromatase, the rate-limiting ... ...

Abstract	In situ estrogen synthesis by hormone-dependent breast cancers could potentially regulate cellular proliferation through autocrine or paracrine mechanisms. Several biochemical studies have demonstrated activity of the enzyme aromatase, the rate-limiting step for estrogen synthesis, in breast tumor homogenates. Prior immunohistochemical studies in breast neoplasms demonstrated aromatase antibody binding to both stroma and parenchyma, but biochemically measured enzyme activity significantly correlated only with the level of staining in the stromal component. The present study sought to provide more direct evidence of the predominant role for stromal cell aromatase in breast tumor tissue. Accordingly, breast tumor stromal and epithelial cells were examined for aromatase enzyme activity and messenger ribonucleic acid (mRNA) expression. Stromal and epithelial cells from benign tissue served as a means of comparing activity and regulation in benign and tumor tissue. Enzyme activity in stromal cells from breast tumor tissue was low basally, but increased by 30- to 1200-fold when induced by dexamethasone. Combining dexamethasone with phorbol esters and cAMP produced an additional 1.2- to 4.1-fold stimulation. Analyses of exons III/V and exons IX/X demonstrated that aromatase mRNA expression was also substantially increased by these treatments. Increases in enzyme activity and mRNA expression in cells from benign breast stroma paralleled those observed in tumor stroma, although the increases in enzyme activity were generally lower. In contrast to the responses observed in stromal cells, epithelial cells from breast tumor or nonmalignant breast tissue were unresponsive to dexamethasone, either added alone or in combination with phorbol esters and cAMP. This study provides direct biochemical evidence that aromatase is present in stroma within breast tumors, as in surrounding tissues, and suggests that estrogen synthesis within the tumor may modulate tumor growth via a paracrine mechanism.
MeSH term(s)	Aged ; Aromatase/genetics ; Aromatase/metabolism ; Breast/enzymology ; Breast Neoplasms/enzymology ; Bucladesine/pharmacology ; Dexamethasone/pharmacology ; Epithelium/enzymology ; Female ; Gene Expression/drug effects ; Humans ; Middle Aged ; Phorbol Esters/pharmacology ; RNA, Messenger/metabolism ; Stromal Cells/drug effects ; Stromal Cells/enzymology ; Tumor Cells, Cultured
Chemical Substances	Phorbol Esters ; RNA, Messenger ; phorbol-12,13-diacetate (24928-15-2) ; Bucladesine (63X7MBT2LQ) ; Dexamethasone (7S5I7G3JQL) ; Aromatase (EC 1.14.14.1)
Language	English
Publishing date	1997-01
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	3029-6
ISSN	1945-7197 ; 0021-972X
ISSN (online)	1945-7197
ISSN	0021-972X
DOI	10.1210/jcem.82.1.3672
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Article: [Lymphocyte subsets and beta-family chemokines in the BALF of patients with diffuse panbronchitis before and after treatment with macrolide antibiotics].

Iida, K / Kawakami, K / Shirai, R / Matsubara, Y / Taniguchi, H / Kawamoto, T / Fujii, T / Kaseta, M / Monda, J / Kawano, S

The Japanese journal of antibiotics

1997 Volume 50 Suppl A, Page(s) 116–119

MeSH term(s)	Adult ; Anti-Bacterial Agents/therapeutic use ; Bronchitis/drug therapy ; Bronchitis/immunology ; Bronchoalveolar Lavage Fluid ; Chemokine CCL2/analysis ; Chemokine CCL5/analysis ; Humans ; Lymphocyte Subsets/immunology ; Macrolides
Chemical Substances	Anti-Bacterial Agents ; Chemokine CCL2 ; Chemokine CCL5 ; Macrolides
Language	Japanese
Publishing date	1997-03
Publishing country	Japan
Document type	Journal Article
ZDB-ID	390804-5
ISSN	0368-2781
ISSN	0368-2781
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Article: [Study of therapeutic effects of macrolide antibiotics on patients with anti-HTLV-1 antibody positive DPB (diffuse panbronchitis)].

Kaseta, M / Fujii, T / Taniguchi, H / Kawamoto, T / Shirai, R / Iida, K / Kawakami, K / Matsubara, Y / Kadota, J / Kawano, S

The Japanese journal of antibiotics

1997 Volume 50 Suppl A, Page(s) 134–136

MeSH term(s)	Anti-Bacterial Agents/therapeutic use ; Bronchitis/complications ; Bronchitis/drug therapy ; Clarithromycin/therapeutic use ; Erythromycin/therapeutic use ; Female ; HIV Seropositivity/complications ; HIV-1/immunology ; Humans ; Male ; Middle Aged ; Roxithromycin/therapeutic use
Chemical Substances	Anti-Bacterial Agents ; Roxithromycin (21KOF230FA) ; Erythromycin (63937KV33D) ; Clarithromycin (H1250JIK0A)
Language	Japanese
Publishing date	1997-03
Publishing country	Japan
Document type	Comparative Study ; Journal Article
ZDB-ID	390804-5
ISSN	0368-2781
ISSN	0368-2781
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Article: Estrogen production via the aromatase enzyme in breast carcinoma: which cell type is responsible?

Santen, R J / Santner, S J / Pauley, R J / Tait, L / Kaseta, J / Demers, L M / Hamilton, C / Yue, W / Wang, J P

The Journal of steroid biochemistry and molecular biology

1997 Volume 61, Issue 3-6, Page(s) 267–271

Abstract: Studies of breast tumor homogenates from women with breast cancer have demonstrated the synthesis of estrogens in situ through the enzyme aromatase. The present series of investigations sought to determine which cell type within the tumor is responsible ... ...

Abstract	Studies of breast tumor homogenates from women with breast cancer have demonstrated the synthesis of estrogens in situ through the enzyme aromatase. The present series of investigations sought to determine which cell type within the tumor is responsible for local estrogen biosynthesis, and whether or not the amount produced is biologically important. Accordingly, we utilized an indirect immunohistochemical scoring method (H-score) to determine the relative amount of enzyme present in tumor epithelial and stromal cells. This revealed a value of 13 for tumor stromal cells and 4.8 for the epithelial component. Contributing to this difference is the fact that a greater percentage of cells in the tumor were stromal (45%) than epithelial (37%). To obtain direct evidence that tumor stromal cells could synthesize estrogens, we isolated and grew these cells in tissue culture. Stromal cells originating from within the tumor could be stimulated by known enhancers of transcription to produce nearly as much aromatase as is found in placental microsomes. Stromal cells isolated from benign tissue distal to the tumor exhibited properties similar to those of the tumor stroma. Epithelial cells, in contrast, did not respond to these enhancers and had low levels of aromatase basally. To obtain proof of the principle that local estrogen synthesis can be biologically meaningful, we measured tumor tissue estradiol levels and growth rates in aromatase-transfected MCF-7 cells implanted into nude mice. Local synthesis resulted in tumor levels ranging from 300 to 800 pg/g and growth rates substantially higher than in non-aromatase-containing tumors. These data suggest that tumor stromal cells contribute the major portion of estrogen synthesized in tumors, and that this local synthesis can increase tumor estradiol levels and growth rates.
MeSH term(s)	Animals ; Aromatase/metabolism ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Epithelium/metabolism ; Estrogens/biosynthesis ; Female ; Humans ; Immunohistochemistry ; Mice ; Stromal Cells/metabolism
Chemical Substances	Estrogens ; Aromatase (EC 1.14.14.1)
Language	English
Publishing date	1997-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1049188-0
ISSN	1879-1220 ; 0960-0760
ISSN (online)	1879-1220
ISSN	0960-0760
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