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  1. Article ; Online: Quality of inpatient paediatric and newborn care in district hospitals: WHO indicators, measurement, and improvement.

    English, Mike / Aluvaala, Jalemba / Maina, Michuki / Duke, Trevor / Irimu, Grace

    The Lancet. Global health

    2023  Volume 11, Issue 7, Page(s) e1114–e1119

    Abstract: Poor-quality paediatric and neonatal care in district hospitals in low-income and middle-income countries (LMICs) was first highlighted more than 20 years ago. WHO recently developed more than 1000 paediatric and neonatal quality indicators for hospitals. ...

    Abstract Poor-quality paediatric and neonatal care in district hospitals in low-income and middle-income countries (LMICs) was first highlighted more than 20 years ago. WHO recently developed more than 1000 paediatric and neonatal quality indicators for hospitals. Prioritising these indicators should account for the challenges in producing reliable process and outcome data in these settings, and their measurement should not unduly narrow the focus of global and national actors to reports of measured indicators. A three-tier, long-term strategy for the improvement of paedicatric and neonatal care in LMIC district hospitals is needed, comprising quality measurement, governance, and front-line support. Measurement should be better supported by integrating data from routine information systems to reduce the future cost of surveys. Governance and quality management processes need to address system-wide issues and develop supportive institutional norms and organisational culture. This strategy requires governments, regulators, professions, training institutions, and others to engage beyond the initial consultation on indicator selection, and to tackle the pervasive constraints that undermine the quality of district hospital care. Institutional development must be combined with direct support to hospitals. Too often the focus of indicator measurement as an improvement strategy is on reporting up to regional or national managers, but not on providing support down to hospitals to attain quality care.
    MeSH term(s) Infant, Newborn ; Child ; Humans ; Hospitals, District ; Inpatients ; Quality of Health Care ; World Health Organization
    Language English
    Publishing date 2023-05-23
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2723488-5
    ISSN 2214-109X ; 2214-109X
    ISSN (online) 2214-109X
    ISSN 2214-109X
    DOI 10.1016/S2214-109X(23)00190-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Quality of inpatient paediatric and newborn care in district hospitals - Authors' reply.

    English, Mike / Aluvaala, Jalemba / Maina, Michuki / Duke, Trevor / Irimu, Grace

    The Lancet. Global health

    2023  Volume 11, Issue 10, Page(s) e1514–e1515

    MeSH term(s) Infant, Newborn ; Humans ; Child ; Inpatients ; Hospitals, District
    Language English
    Publishing date 2023-09-21
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 2723488-5
    ISSN 2214-109X ; 2214-109X
    ISSN (online) 2214-109X
    ISSN 2214-109X
    DOI 10.1016/S2214-109X(23)00370-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The clinical trials group turns 20: a clinician's perspective.

    Duke, G J

    Anaesthesia and intensive care

    2014  Volume 42, Issue 5, Page(s) 575–578

    Abstract: A clinician's perspective on the first 20 years of the Australian and New Zealand Intensive Care Society Clinical Trials Group and its influence on intensive care clinical practice over this same time period. This point of view discusses the importance ... ...

    Abstract A clinician's perspective on the first 20 years of the Australian and New Zealand Intensive Care Society Clinical Trials Group and its influence on intensive care clinical practice over this same time period. This point of view discusses the importance of the Clinical Trials Group and the significance of several major published research trials.
    MeSH term(s) Australia ; Clinical Trials as Topic ; Humans ; New Zealand
    Language English
    Publishing date 2014-09-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 187524-3
    ISSN 1448-0271 ; 0310-057X
    ISSN (online) 1448-0271
    ISSN 0310-057X
    DOI 10.1177/0310057X1404200505
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Multiscale computational model predicts how environmental changes and drug treatments affect microvascular remodeling in fibrotic disease.

    Leonard-Duke, Julie / Agro, Samuel M J / Csordas, David J / Bruce, Anthony C / Eggertsen, Taylor G / Tavakol, Tara N / Barker, Thomas H / Bonham, Catherine A / Saucerman, Jeffery J / Taite, Lakeshia J / Peirce, Shayn M

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Investigating the molecular, cellular, and tissue-level changes caused by disease, and the effects of pharmacological treatments across these biological scales, necessitates the use of multiscale computational modeling in combination with experimentation. ...

    Abstract Investigating the molecular, cellular, and tissue-level changes caused by disease, and the effects of pharmacological treatments across these biological scales, necessitates the use of multiscale computational modeling in combination with experimentation. Many diseases dynamically alter the tissue microenvironment in ways that trigger microvascular network remodeling, which leads to the expansion or regression of microvessel networks. When microvessels undergo remodeling in idiopathic pulmonary fibrosis (IPF), functional gas exchange is impaired due to loss of alveolar structures and lung function declines. Here, we integrated a multiscale computational model with independent experiments to investigate how combinations of biomechanical and biochemical cues in IPF alter cell fate decisions leading to microvascular remodeling. Our computational model predicted that extracellular matrix (ECM) stiffening reduced microvessel area, which was accompanied by physical uncoupling of endothelial cell (ECs) and pericytes, the cells that comprise microvessels. Nintedanib, an FDA-approved drug for treating IPF, was predicted to further potentiate microvessel regression by decreasing the percentage of quiescent pericytes while increasing the percentage of pericytes undergoing pericyte-myofibroblast transition (PMT) in high ECM stiffnesses. Importantly, the model suggested that YAP/TAZ inhibition may overcome the deleterious effects of nintedanib by promoting EC-pericyte coupling and maintaining microvessel homeostasis. Overall, our combination of computational and experimental modeling can explain how cell decisions affect tissue changes during disease and in response to treatments.
    Language English
    Publishing date 2024-03-22
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.15.585249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Expanding the molecular and phenotypic spectrum of CTLA-4 insufficiency.

    Duke, Sean / Maiarana, James / Yousefi, Pariya / Burks, Elijah / Gerrie, Samantha / Setiadi, Audi / Hildebrand, Kyla J / James, Elliot / Turvey, Stuart E / Markle, Janet G / Biggs, Catherine M

    Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

    2024  Volume 35, Issue 2, Page(s) e14077

    MeSH term(s) Humans ; CTLA-4 Antigen
    Chemical Substances CTLA-4 Antigen
    Language English
    Publishing date 2024-02-13
    Publishing country England
    Document type Letter
    ZDB-ID 1057059-7
    ISSN 1399-3038 ; 0905-6157 ; 0906-5784
    ISSN (online) 1399-3038
    ISSN 0905-6157 ; 0906-5784
    DOI 10.1111/pai.14077
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: High Throughput Tomography (HiTT) on EMBL beamline P14 on PETRA III.

    Albers, Jonas / Nikolova, Marina / Svetlove, Angelika / Darif, Nedal / Lawson, Matthew J / Schneider, Thomas R / Schwab, Yannick / Bourenkov, Gleb / Duke, Elizabeth

    Journal of synchrotron radiation

    2024  Volume 31, Issue Pt 1, Page(s) 186–194

    Abstract: Here, high-throughput tomography (HiTT), a fast and versatile phase-contrast imaging platform for life-science samples on the EMBL beamline P14 at DESY in Hamburg, Germany, is presented. A high-photon-flux undulator beamline is used to perform ... ...

    Abstract Here, high-throughput tomography (HiTT), a fast and versatile phase-contrast imaging platform for life-science samples on the EMBL beamline P14 at DESY in Hamburg, Germany, is presented. A high-photon-flux undulator beamline is used to perform tomographic phase-contrast acquisition in about two minutes which is linked to an automated data processing pipeline that delivers a 3D reconstructed data set less than a minute and a half after the completion of the X-ray scan. Combining this workflow with a sophisticated robotic sample changer enables the streamlined collection and reconstruction of X-ray imaging data from potentially hundreds of samples during a beam-time shift. HiTT permits optimal data collection for many different samples and makes possible the imaging of large sample cohorts thus allowing population studies to be attempted. The successful application of HiTT on various soft tissue samples in both liquid (hydrated and also dehydrated) and paraffin-embedded preparations is demonstrated. Furthermore, the feasibility of HiTT to be used as a targeting tool for volume electron microscopy, as well as using HiTT to study plant morphology, is demonstrated. It is also shown how the high-throughput nature of the work has allowed large numbers of `identical' samples to be imaged to enable statistically relevant sample volumes to be studied.
    MeSH term(s) Synchrotrons ; X-Rays ; Tomography, X-Ray Computed ; Robotics ; Germany
    Language English
    Publishing date 2024-01-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2021413-3
    ISSN 1600-5775 ; 0909-0495
    ISSN (online) 1600-5775
    ISSN 0909-0495
    DOI 10.1107/S160057752300944X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Considering Functional Outcomes as Efficacy Endpoints in Pediatric Low-Grade Glioma Clinical Trials: An FDA Educational Symposium.

    Fangusaro, Jason / Avery, Robert A / Fisher, Michael J / Packer, Roger J / Walsh, Karin S / Schouten-van Meeteren, Antoinette / Karres, Dominik / Bradford, Diana / Bhatnagar, Vishal / Singh, Harpreet / Kluetz, Paul G / Donoghue, Martha / Duke, Elizabeth S

    Clinical cancer research : an official journal of the American Association for Cancer Research

    2024  

    Abstract: In October 2022, the Food and Drug Administration (FDA) Oncology Center of Excellence (OCE) hosted an educational symposium entitled, "Considering Functional Outcomes as Efficacy Endpoints in Pediatric Low-Grade Glioma (pLGG) Clinical Trials." The ... ...

    Abstract In October 2022, the Food and Drug Administration (FDA) Oncology Center of Excellence (OCE) hosted an educational symposium entitled, "Considering Functional Outcomes as Efficacy Endpoints in Pediatric Low-Grade Glioma (pLGG) Clinical Trials." The symposium brought together patient advocates, regulators from the FDA and the European Medicines Agency (EMA), and an international group of academic thought leaders in the field of pediatric neuro-oncology to discuss the potential role of functional outcomes, including visual acuity, motor function, and neurocognitive performance, as endpoints in clinical trials enrolling patients with pLGG. The panel discussed challenges and opportunities regarding the selection, implementation, and evaluation of clinical outcome assessments in these functional domains and outlined key considerations for their inclusion in future clinical trial design and role in new drug development.
    Language English
    Publishing date 2024-02-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1225457-5
    ISSN 1557-3265 ; 1078-0432
    ISSN (online) 1557-3265
    ISSN 1078-0432
    DOI 10.1158/1078-0432.CCR-23-3386
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Distinct subpopulations of D1 medium spiny neurons exhibit unique transcriptional responsiveness to cocaine.

    Phillips, Robert A / Tuscher, Jennifer J / Fitzgerald, N Dalton / Wan, Ethan / Zipperly, Morgan E / Duke, Corey G / Ianov, Lara / Day, Jeremy J

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Drugs of abuse increase extracellular concentrations of dopamine in the nucleus accumbens (NAc), resulting in transcriptional alterations that drive long-lasting cellular and behavioral adaptations. While decades of research have focused on the ... ...

    Abstract Drugs of abuse increase extracellular concentrations of dopamine in the nucleus accumbens (NAc), resulting in transcriptional alterations that drive long-lasting cellular and behavioral adaptations. While decades of research have focused on the transcriptional mechanisms by which drugs of abuse influence neuronal physiology and function, few studies have comprehensively defined NAc cell type heterogeneity in transcriptional responses to drugs of abuse. Here, we used single nucleus RNA-seq (snRNA-seq) to characterize the transcriptome of over 39,000 NAc cells from male and female adult Sprague-Dawley rats following acute or repeated cocaine experience. This dataset identified 16 transcriptionally distinct cell populations, including two populations of medium spiny neurons (MSNs) that express the Drd1 dopamine receptor (D1-MSNs). Critically, while both populations expressed classic marker genes of D1-MSNs, only one population exhibited a robust transcriptional response to cocaine. Validation of population-selective transcripts using RNA in situ hybridization revealed distinct spatial compartmentalization of these D1-MSN populations within the NAc. Finally, analysis of published NAc snRNA-seq datasets from non-human primates and humans demonstrated conservation of MSN subtypes across rat and higher order mammals, and further highlighted cell type-specific transcriptional differences across the NAc and broader striatum. These results highlight the utility in using snRNA-seq to characterize both cell type heterogeneity and cell type-specific responses to cocaine and provides a useful resource for cross-species comparisons of NAc cell composition.
    Language English
    Publishing date 2023-01-13
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.01.12.523845
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Distinct subpopulations of D1 medium spiny neurons exhibit unique transcriptional responsiveness to cocaine.

    Phillips, Robert A / Tuscher, Jennifer J / Fitzgerald, N Dalton / Wan, Ethan / Zipperly, Morgan E / Duke, Corey G / Ianov, Lara / Day, Jeremy J

    Molecular and cellular neurosciences

    2023  Volume 125, Page(s) 103849

    Abstract: Drugs of abuse increase extracellular concentrations of dopamine in the nucleus accumbens (NAc), resulting in transcriptional alterations that drive long-lasting cellular and behavioral adaptations. While decades of research have focused on the ... ...

    Abstract Drugs of abuse increase extracellular concentrations of dopamine in the nucleus accumbens (NAc), resulting in transcriptional alterations that drive long-lasting cellular and behavioral adaptations. While decades of research have focused on the transcriptional mechanisms by which drugs of abuse influence neuronal physiology and function, few studies have comprehensively defined NAc cell type heterogeneity in transcriptional responses to drugs of abuse. Here, we used single nucleus RNA-seq (snRNA-seq) to characterize the transcriptome of over 39,000 NAc cells from male and female adult Sprague-Dawley rats following acute or repeated cocaine experience. This dataset identified 16 transcriptionally distinct cell populations, including two populations of medium spiny neurons (MSNs) that express the Drd1 dopamine receptor (D1-MSNs). Critically, while both populations expressed classic marker genes of D1-MSNs, only one population exhibited a robust transcriptional response to cocaine. Validation of population-selective transcripts using RNA in situ hybridization revealed distinct spatial compartmentalization of these D1-MSN populations within the NAc. Finally, analysis of published NAc snRNA-seq datasets from non-human primates and humans demonstrated conservation of MSN subtypes across rat and higher order mammals, and further highlighted cell type-specific transcriptional differences across the NAc and broader striatum. These results highlight the utility in using snRNA-seq to characterize both cell type heterogeneity and cell type-specific responses to cocaine and provides a useful resource for cross-species comparisons of NAc cell composition.
    MeSH term(s) Male ; Female ; Rats ; Animals ; Mice ; Cocaine/pharmacology ; Medium Spiny Neurons ; Receptors, Dopamine D2/genetics ; Receptors, Dopamine D2/metabolism ; Rats, Sprague-Dawley ; Neurons/metabolism ; Receptors, Dopamine D1/genetics ; Receptors, Dopamine D1/metabolism ; Nucleus Accumbens/metabolism ; Mice, Inbred C57BL ; Mice, Transgenic ; Mammals
    Chemical Substances Cocaine (I5Y540LHVR) ; Receptors, Dopamine D2 ; Receptors, Dopamine D1
    Language English
    Publishing date 2023-03-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1046640-x
    ISSN 1095-9327 ; 1044-7431
    ISSN (online) 1095-9327
    ISSN 1044-7431
    DOI 10.1016/j.mcn.2023.103849
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Correction to: Oncogenic RIT1 mutations in lung adenocarcinoma.

    Berger, A H / Imielinski, M / Duke, F / Wala, J / Kaplan, N / Shi, G -X / Andres, D A / Meyerson, M

    Oncogene

    2022  Volume 41, Issue 19, Page(s) 2788

    Language English
    Publishing date 2022-04-13
    Publishing country England
    Document type Published Erratum
    ZDB-ID 639046-8
    ISSN 1476-5594 ; 0950-9232
    ISSN (online) 1476-5594
    ISSN 0950-9232
    DOI 10.1038/s41388-022-02300-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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