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Article ; Online: HLA-G and Recurrent Pregnancy Loss.

Barbaro, Greta / Inversetti, Annalisa / Cristodoro, Martina / Ticconi, Carlo / Scambia, Giovanni / Di Simone, Nicoletta

International journal of molecular sciences

2023 Volume 24, Issue 3

Abstract: ... not completely understood, Human Leukocyte Antigen G (HLA-G), expressed on trophoblastic cell surfaces ... the HLA-G pathway alterations are involved in disrupting the immunologic balance and in increasing the risk ...

Abstract	Placentation is an immunological compromise where maternal immune system cells and trophoblastic cells interact to reach an equilibrium condition. Although the cross talk between the two systems is complex and not completely understood, Human Leukocyte Antigen G (HLA-G), expressed on trophoblastic cell surfaces, seems to be one of the main molecules involved in the modulation of both local and systemic maternal immune response. The prevalence of recurrent pregnancy loss (RPL), probably underestimated, is 5% of all women who achieve pregnancy, and about 40-60% percent of RPL cases are unexplained. There is an immunological analogy between allograft rejection and miscarriage, and the purpose of this review is to describe how the HLA-G pathway alterations are involved in disrupting the immunologic balance and in increasing the risk of recurrent pregnancy loss.
MeSH term(s)	Pregnancy ; Female ; Humans ; HLA-G Antigens/genetics ; Abortion, Habitual ; Placentation ; Trophoblasts/metabolism
Chemical Substances	HLA-G Antigens
Language	English
Publishing date	2023-01-29
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24032557
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Article ; Online: HLA-G and Recurrent Pregnancy Loss

Greta Barbaro / Annalisa Inversetti / Martina Cristodoro / Carlo Ticconi / Giovanni Scambia / Nicoletta Di Simone

International Journal of Molecular Sciences, Vol 24, Iss 2557, p

2023 Volume 2557

Abstract	Placentation is an immunological compromise where maternal immune system cells and trophoblastic cells interact to reach an equilibrium condition. Although the cross talk between the two systems is complex and not completely understood, Human Leukocyte Antigen G (HLA-G), expressed on trophoblastic cell surfaces, seems to be one of the main molecules involved in the modulation of both local and systemic maternal immune response. The prevalence of recurrent pregnancy loss (RPL), probably underestimated, is 5% of all women who achieve pregnancy, and about 40–60% percent of RPL cases are unexplained. There is an immunological analogy between allograft rejection and miscarriage, and the purpose of this review is to describe how the HLA-G pathway alterations are involved in disrupting the immunologic balance and in increasing the risk of recurrent pregnancy loss.
Keywords	HLA-G ; recurrent pregnancy loss ; trophoblast ; placentation ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: New insights into SARS-CoV-2 Lumipulse G salivary antigen testing: accuracy, safety and short TAT enhance surveillance.

Aita, Ada / Navaglia, Filippo / Moz, Stefania / Contran, Nicole / Barbaro, Francesco / Cattelan, Anna Maria / Padoan, Andrea / Cosma, Chiara / Faggian, Diego / Plebani, Mario / Basso, Daniela

Clinical chemistry and laboratory medicine

2022 Volume 61, Issue 2, Page(s) 323–331

Abstract: ... TaqPath rRT-PCR) and chemiluminescent Ag assays (Lumipulse G). The effect of sample pre-treatment ...

Abstract	Objectives: The rapid, accurate and safe detection of SARS-CoV-2 is the key to improving surveillance and infection containment. The aim of the present study was to ascertain whether, after heat/chemical inactivation, SARS-CoV-2 N antigen chemiluminescence (CLEIA) assay in saliva remains a valid alternative to molecular testing. Methods: In 2022, 139 COVID-19 inpatients and 467 healthcare workers were enrolled. In 606 self-collected saliva samples (Salivette), SARS-CoV-2 was detected by molecular (TaqPath rRT-PCR) and chemiluminescent Ag assays (Lumipulse G). The effect of sample pre-treatment (extraction solution-ES or heating) on antigen recovery was verified. Results: Salivary SARS-CoV-2 antigen assay was highly accurate (AUC=0.959, 95% CI: 0.943-0.974), with 90% sensitivity and 92% specificity. Of the 254 antigen positive samples, 29 were false positives. We demonstrated that heterophilic antibodies could be a cause of false positive results. A significant antigen concentration decrease was observed after ES treatment (p=0.0026), with misclassification of 43 samples. Heat had a minimal impact, after treatment the correct classification of cases was maintained. Conclusions: CLEIA SARS-CoV-2 salivary antigen provides accurate, timely and high-throughput results that remain accurate also after heat inactivation, thus ensuring a safer work environment. This supports the use of salivary antigen detection by CLEIA in surveillance programs.
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19/diagnosis ; COVID-19 Testing ; Saliva ; Sensitivity and Specificity
Language	English
Publishing date	2022-10-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2022-0849
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Article ; Online: 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein-coupled receptor D.

Science signaling

2018 Volume 11, Issue 561

Abstract: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is characterized by chronic abdominal pain concurrent with altered bowel habit. Polyunsaturated fatty acid (PUFA) metabolites are increased in abundance in IBS and are implicated ... ...

Abstract	Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is characterized by chronic abdominal pain concurrent with altered bowel habit. Polyunsaturated fatty acid (PUFA) metabolites are increased in abundance in IBS and are implicated in the alteration of sensation to mechanical stimuli, which is defined as visceral hypersensitivity. We sought to quantify PUFA metabolites in patients with IBS and evaluate their role in pain. Quantification of PUFA metabolites by mass spectrometry in colonic biopsies showed an increased abundance of 5-oxoeicosatetraenoic acid (5-oxoETE) only in biopsies taken from patients with IBS with predominant constipation (IBS-C). Local administration of 5-oxoETE to mice induced somatic and visceral hypersensitivity to mechanical stimuli without causing tissue inflammation. We found that 5-oxoETE directly acted on both human and mouse sensory neurons as shown by lumbar splanchnic nerve recordings and Ca
MeSH term(s)	Animals ; Arachidonic Acids/metabolism ; Calcium/metabolism ; Case-Control Studies ; Colon/drug effects ; Colon/metabolism ; Colon/pathology ; Constipation/chemically induced ; Constipation/physiopathology ; Ganglia, Spinal/drug effects ; Ganglia, Spinal/metabolism ; Ganglia, Spinal/pathology ; Humans ; Irritable Bowel Syndrome/etiology ; Irritable Bowel Syndrome/metabolism ; Irritable Bowel Syndrome/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Nociception ; Receptors, G-Protein-Coupled/physiology ; Sensory Receptor Cells/drug effects ; Sensory Receptor Cells/metabolism ; Sensory Receptor Cells/pathology ; Signal Transduction
Chemical Substances	Arachidonic Acids ; Mrgprd protein, mouse ; Receptors, G-Protein-Coupled ; 5-oxo-6,8,11,14-eicosatetraenoic acid (126432-17-5) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2018-12-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2417226-1
ISSN	1937-9145 ; 1945-0877
ISSN (online)	1937-9145
ISSN	1945-0877
DOI	10.1126/scisignal.aal2171
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Article ; Online: CYP2D6 -1584C>G promoter polymorphism and debrisoquine ultrarapid hydroxylation in healthy volunteers.

Llerena, Adrian / Dorado, Pedro / Ramírez, Ronald / Calzadilla, Luis R / Peñas-Lledó, Eva / Álvarez, Mayra / Naranjo, María E G / González, Idilio / Pérez, Bárbaro

Pharmacogenomics

2013 Volume 14, Issue 16, Page(s) 1973–1977

Abstract: Background & aim: The CYP2D6 -1584C>G polymorphism (rs1080985) has been identified as a major ... metabolism. The objective of the present study was to explore the relationship between the CYP2D6 -1584C>G ... impact on the ultrarapid CYP2D6 hydroxylation capacity.: Materials & methods: The CYP2D6 -1584C>G ...

Abstract	Background & aim: The CYP2D6 -1584C>G polymorphism (rs1080985) has been identified as a major factor for CYP2D6 expression and function, with the mutant -1584G promoter type being consistently associated with significantly greater expression than -1584C. It may therefore be associated with ultrarapid metabolism. The objective of the present study was to explore the relationship between the CYP2D6 -1584C>G polymorphism and the debrisoquine metabolic ratio in healthy volunteers in order to evaluate its potential impact on the ultrarapid CYP2D6 hydroxylation capacity. Materials & methods: The CYP2D6 -1584C>G polymorphism was analyzed in 320 unrelated healthy individuals who were previously phenotyped for debrisoquine hydroxylation. Results: The metabolic ratio (log10 mean ± standard deviation) of individuals with the -1584G allele was lower than that of individuals with the -1584C allele for carriers of one active CYP2D6 gene (-0.13 ± 0.33 and 0.17 ± 0.52, respectively; p < 0.05) or two active CYP2D6 genes (-0.32 ± 0.39 and -0.20 ± 0.44, respectively; p < 0.05). Conclusion: The presence of the -1584G allele in the promoter region of the CYP2D6 gene was related to a high CYP2D6 hydroxylation capacity.
MeSH term(s)	Alleles ; Cytochrome P-450 CYP2D6/genetics ; Debrisoquin/therapeutic use ; Gene Expression Regulation/drug effects ; Genotype ; Healthy Volunteers ; Heterozygote ; Humans ; Hydroxylation/drug effects ; Hydroxylation/genetics ; Polymorphism, Genetic ; Promoter Regions, Genetic
Chemical Substances	Cytochrome P-450 CYP2D6 (EC 1.14.14.1) ; Debrisoquin (X31CDK040E)
Language	English
Publishing date	2013-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2019513-8
ISSN	1744-8042 ; 1462-2416
ISSN (online)	1744-8042
ISSN	1462-2416
DOI	10.2217/pgs.13.181
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Article ; Online: Adiponectin -11377C/G and +276G/T polymorphisms affect adiponectin levels but do not modify responsiveness to therapy in resistant hypertension.

de Faria, Ana Paula C / Modolo, Rodrigo / Sabbatini, Andréa R / Barbaro, Natália R / Corrêa, Nathália B / Brunelli, Veridiana / Tanus-Santos, José E / Fontana, Vanessa / Moreno, Heitor

Basic & clinical pharmacology & toxicology

2015 Volume 117, Issue 1, Page(s) 65–72

Abstract: ... with RHTN. Single nucleotide polymorphisms (SNPs) -11377C/G (rs266729) and +276G/T (rs1501299) in ADIPOQ ... 11377C/G and +276G/T) and adiponectin levels in RHTN. This study comprised 109 patients with RHTN ... versus G allele carriers for -11377C/G and GG homozygous versus T allele carriers for +276G/T. Office and ...

Abstract	Resistant hypertension (RHTN) is a multifactorial and polygenic disease, frequently associated with obesity. Low plasma adiponectin levels, a hormone produced by the adipose tissue, were associated with RHTN. Single nucleotide polymorphisms (SNPs) -11377C/G (rs266729) and +276G/T (rs1501299) in ADIPOQ (adiponectin gene) were associated with hypertension. This study evaluated the association between two SNPs (-11377C/G and +276G/T) and adiponectin levels in RHTN. This study comprised 109 patients with RHTN genotyped for both polymorphisms. A cross-sectional study was designed to compare features of CC homozygous versus G allele carriers for -11377C/G and GG homozygous versus T allele carriers for +276G/T. Office and ambulatory BP measurements were similar among genotypes subgroups in both SNPs as well as the markers of target organ damage (arterial stiffness, left ventricular mass index and microalbuminuria). Adiponectin concentrations were significantly higher in CC compared to G carrier for -11377C/G (CC:7.0 (4.0-10.2) versus G allele:5.5 (2.5-7.9), p = 0.04) and lower in GG compared to T carrier for +276G/T (GG:5.3 (2.3-7.7) versus T allele:7.1 (3.6-10.5), p = 0.04). Adjusting for systolic ambulatory BP, body mass index, age, gender, race and presence of type 2 diabetes, multiple linear regression analyses revealed that the minor alleles G (β-coefficient= -0.14, SE=0.07, p = 0.03) and T (β-coefficient=0.12, SE=0.06, p = 0.04) were independent predictors of adiponectin. The -11377C/G and +276G/T SNPs in ADIPOQ were associated with adiponectin levels in RHTN individuals.
MeSH term(s)	Adiponectin/blood ; Adiponectin/genetics ; Aged ; Antihypertensive Agents/therapeutic use ; Biomarkers/blood ; Blood Pressure/drug effects ; Blood Pressure/genetics ; Cross-Sectional Studies ; Drug Resistance ; Female ; Genetic Predisposition to Disease ; Heterozygote ; Homozygote ; Humans ; Hypertension/blood ; Hypertension/drug therapy ; Hypertension/genetics ; Hypertension/physiopathology ; Male ; Middle Aged ; Phenotype ; Polymorphism, Single Nucleotide ; Risk Factors
Chemical Substances	ADIPOQ protein, human ; Adiponectin ; Antihypertensive Agents ; Biomarkers
Language	English
Publishing date	2015-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2134679-3
ISSN	1742-7843 ; 1742-7835
ISSN (online)	1742-7843
ISSN	1742-7835
DOI	10.1111/bcpt.12368
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Article ; Online: Energy Communities in Urban Areas: Comparison of Energy Strategy and Economic Feasibility in Italy and Spain

Barbaro, Simona / Napoli, Grazia

Land. 2023 June 24, v. 12, no. 7

2023

Abstract: ... the most relevant cost-effectiveness indicators, e.g., Net Present Value. It turns out that a project ...

Abstract	Energy communities using renewable energy sources directly contributes to reduction of climate change gas emissions and energy consumption in the European Union. In addition, energy communities enable citizens to transform from (passive) consumers to prosumers (active consumers and producers) and to play a proactive role in the deployment of energy transition in urban areas. As the transposition of European rules about energy communities into the national laws of EU Member States is very articulated and differentiated, this study proposes a framework to analyze and compare regulatory and financial instruments. This framework is applied to the analysis of the cases of Italy and Spain as representative of European states in which collective actions in the energy sector are not very common, with the aim of highlighting the main critical issues affecting the effectiveness of energy transition strategies and assessing the economic feasibility of energy communities. Based on analysis of regulations and procedures, including at the local level, it appears that municipalities play an important role as promoters of initiatives among citizen communities, while complex bureaucratic procedure is the most critical issue in both countries and can significantly hinder the spread of energy communities. With respect to the different financial incentives available for the formation of energy communities in Italy and Spain, a few cases studies are hypothesized, calculating the most relevant cost-effectiveness indicators, e.g., Net Present Value. It turns out that a project with the same characteristics achieves greater economic feasibility in Italy than in Spain, depending on the type and size of incentives set by national laws and, above all, that financial incentives are necessary to make the formation of energy communities cost-effective and thus to achieve direct citizen involvement in energy transition actions.
Keywords	European Union ; climate change ; cost effectiveness ; economic feasibility ; energy ; energy industry ; land ; Italy ; Spain
Language	English
Dates of publication	2023-0624
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2682955-1
ISSN	2073-445X
ISSN	2073-445X
DOI	10.3390/land12071282
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Article ; Online: Leisure, Employment, Community Participation, and Quality of Life in Primary Caregivers of Autistic Children: A Qualitative Study.

Davy, Gemma / Barbaro, Josephine / Unwin, Katy / Dissanayake, Cheryl

Journal of autism and developmental disorders

2023

Abstract: Purpose: In prioritising the needs of their autistic children, parents often modify their own participation across leisure, social, and workforce activities. Few studies have examined the impact these modifications have on caregiver quality of life (QoL) ...

Abstract	Purpose: In prioritising the needs of their autistic children, parents often modify their own participation across leisure, social, and workforce activities. Few studies have examined the impact these modifications have on caregiver quality of life (QoL). The aim in the current study was to examine how parenting their autistic child/ren impacts parent's participation and QoL. Methods: Twenty primary caregivers (29-48 years, all female) of autistic children (7-11 years) were interviewed online about their participation in leisure, community, and employment activities including the impact of COVID-19. Results: Five themes with underlying subthemes were identified using reflexive thematic analysis. The themes were: (1) Reflecting on the important things in life, (2) Getting access to everything needed, (3) Barriers to participation in meaningful activities, (4) Facilitators of participation in meaningful activities, and (5) Participation through the COVID-19 pandemic. Conclusion: The findings highlight the importance of regular participation in meaningful activities for parents of autistic children and the support needed by them, particularly single parents, to achieve balance between meeting caring responsibilities and their own participation needs.
Language	English
Publishing date	2023-05-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	391999-7
ISSN	1573-3432 ; 0162-3257
ISSN (online)	1573-3432
ISSN	0162-3257
DOI	10.1007/s10803-023-05992-x
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Article ; Online: Efficacy and safety of Diclofenac sodium plaster in patients with acute pain of the limbs: a randomized, placebo and active-controlled, double-blind, parallel-group trial.

Pabst, H / Gruber, G / Picciotto, R / Barbaro, B / Giordan, N

European review for medical and pharmacological sciences

2023 Volume 27, Issue 7, Page(s) 3181–3190

Abstract: Objective: The aim of the present study was to assess the safety and efficacy of Diclofenac sodium (DS) 140 mg medicated plaster vs. Diclofenac epolamine (DIEP) 180 mg medicated plaster and placebo plaster, for the treatment of painful disease due to ... ...

Abstract	Objective: The aim of the present study was to assess the safety and efficacy of Diclofenac sodium (DS) 140 mg medicated plaster vs. Diclofenac epolamine (DIEP) 180 mg medicated plaster and placebo plaster, for the treatment of painful disease due to traumatic events of the limbs. Patients and methods: This was a multicenter, phase III study involving 214 patients, aged 18-65 years, affected by painful conditions due to soft tissue injuries. Patients were randomized to DS, DIEP or placebo arms and treated with once-daily application of the plaster for a total treatment period of 7 days. The primary objective was first to demonstrate the non-inferior efficacy of the DS treatment when compared to the reference DIEP treatment and second that both, test and reference treatments, were superior with respect to placebo. The secondary objectives included the evaluation of efficacy, adhesion, safety, and local tolerability of DS in comparison to both DIEP and placebo. Results: The mean visual analog scale (VAS) score decrease for pain at rest was higher in the DS (-17.65 mm) and the DIEP group (-17.5 mm) than in the placebo (-11.3 mm). Both active formulation plasters were associated with a statistically significant pain reduction compared to placebo. No statistically significant differences were observed between DIEP and DS plasters efficacy in relieving pain. Secondary endpoint evaluations supported the primary efficacy results. No serious adverse events (SAEs) were registered, and the most commonly detected adverse events were skin reactions at the application site. Conclusions: The results showed that both the DS 140 mg plaster and the reference DIEP 180 mg plaster are effective in relieving pain and present a good safety profile.
MeSH term(s)	Humans ; Diclofenac/adverse effects ; Acute Pain ; Soft Tissue Injuries/chemically induced ; Soft Tissue Injuries/complications ; Double-Blind Method ; Anti-Inflammatory Agents, Non-Steroidal/adverse effects ; Treatment Outcome
Chemical Substances	diclofenac hydroxyethylpyrrolidine (119623-66-4) ; Diclofenac (144O8QL0L1) ; Anti-Inflammatory Agents, Non-Steroidal
Language	English
Publishing date	2023-04-18
Publishing country	Italy
Document type	Randomized Controlled Trial ; Multicenter Study ; Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202304_31952
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Article ; Online: Big Kids or Little Adults? Acute Lung Injury Research in Adolescents and Young Adults.

Kohne, Joseph G / Iwashyna, Theodore J / Flori, Heidi R / Barbaro, Ryan P

Annals of the American Thoracic Society

2023 Volume 20, Issue 8, Page(s) 1096–1098

MeSH term(s)	Humans ; Adolescent ; Young Adult ; Acute Lung Injury/diagnosis ; Acute Lung Injury/therapy
Language	English
Publishing date	2023-04-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2717461-X
ISSN	2325-6621 ; 1943-5665 ; 2325-6621
ISSN (online)	2325-6621 ; 1943-5665
ISSN	2325-6621
DOI	10.1513/AnnalsATS.202302-097IP
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