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  1. Book: Molecular diagnostics

    Huggett, Jim F. / O'Grady, Justin

    current research and applications

    2014  

    Author's details ed. by Jim F. Huggett and Justin O'Grady
    Language English
    Size XII, 247 S. : Ill., graph. Darst.
    Publisher Caister Acad. Press
    Publishing place Norfolk
    Publishing country Great Britain
    Document type Book
    HBZ-ID HT018188391
    ISBN 978-1-908230-41-6 ; 9781908230645 ; 1-908230-41-X ; 1908230649
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2014 A 2052 available for loan (reading room) Lesesaal EG

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  2. Article ; Online: The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020.

    Huggett, Jim F

    Clinical chemistry

    2020  Volume 66, Issue 8, Page(s) 1012–1029

    Abstract: Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its ... ...

    Abstract Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.
    MeSH term(s) Guidelines as Topic ; Humans ; Nucleic Acids/analysis ; Polymerase Chain Reaction/methods ; Polymerase Chain Reaction/statistics & numerical data ; Publications/standards
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2020-08-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80102-1
    ISSN 1530-8561 ; 0009-9147
    ISSN (online) 1530-8561
    ISSN 0009-9147
    DOI 10.1093/clinchem/hvaa125
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.171: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Digital PCR for the characterization of reference materials.

    Cleveland, Megan H / He, Hua-Jun / Milavec, Mojca / Bae, Young-Kyung / Vallone, Peter M / Huggett, Jim F

    Molecular aspects of medicine

    2024  Volume 96, Page(s) 101256

    Abstract: Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target ...

    Abstract Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials.
    MeSH term(s) Humans ; Polymerase Chain Reaction/methods ; Nucleic Acids ; Real-Time Polymerase Chain Reaction/methods
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2024-02-14
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 197640-0
    ISSN 1872-9452 ; 0098-2997
    ISSN (online) 1872-9452
    ISSN 0098-2997
    DOI 10.1016/j.mam.2024.101256
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1189: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: COVID-19 new diagnostics development: novel detection methods for SARS-CoV-2 infection and considerations for their translation to routine use.

    Huggett, Jim F / Moran-Gilad, Jacob / Lee, J Eugene

    Current opinion in pulmonary medicine

    2021  Volume 27, Issue 3, Page(s) 155–162

    Abstract: Purpose of review: COVID-19 has put the in-vitro-diagnostic community under an unprecedented spotlight, with a global requirement for accurate SARS-CoV-2 tests. This review will outline technological responses to this need and the analytical ... ...

    Abstract Purpose of review: COVID-19 has put the in-vitro-diagnostic community under an unprecedented spotlight, with a global requirement for accurate SARS-CoV-2 tests. This review will outline technological responses to this need and the analytical considerations required for their translation to routine use.
    Recent findings: SARS-CoV-2 diagnostic solutions directly detect the virus or measure host-derived surrogate markers of infection. With pressure upon supply chains for the 'traditional' molecular approaches, a wide variety of analytical tools spanning the molecular, serology, imaging and chemistry space are being developed, including high throughput solutions and simplified near-patient formats.
    Summary: The unique genetic nature of SARS-CoV-2 means high analytical specificity is achievable by most diagnostic formats. However, clinical sensitivity assessment is complicated by wide discrepancies in analytical range and challenges associated with standardising these differences. When coupled with the acute nature of SARS-CoV-2 infection, reported precise metrics of test performance must be questioned. The response to SARS-CoV-2 has delivered considerable diagnostic innovation, but for a technology to be maximised, it must be demonstrably reproducible and fit for purpose. If novel diagnostic solutions for SARS-CoV-2 are to succeed, equally innovative mechanisms are needed to ensure widespread clinical and surveillance application, enabling agreed standards and metrics to ensure comparability.
    MeSH term(s) COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Testing/methods ; Humans ; Inventions ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Translational Research, Biomedical
    Language English
    Publishing date 2021-01-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1285505-4
    ISSN 1531-6971 ; 1070-5287 ; 1078-1641
    ISSN (online) 1531-6971
    ISSN 1070-5287 ; 1078-1641
    DOI 10.1097/MCP.0000000000000768
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4900: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Pushing the Envelope with Clinical Use of Digital PCR.

    Huggett, Jim F / Devonshire, Alison S / Whale, Alexandra S / Cowen, Simon / Foy, Carole A

    Clinical chemistry

    2021  Volume 67, Issue 7, Page(s) 921–923

    MeSH term(s) Humans ; Polymerase Chain Reaction
    Language English
    Publishing date 2021-06-10
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 80102-1
    ISSN 1530-8561 ; 0009-9147
    ISSN (online) 1530-8561
    ISSN 0009-9147
    DOI 10.1093/clinchem/hvab082
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.171: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  6. Article ; Online: RT-qPCR Diagnostics: The "Drosten" SARS-CoV-2 Assay Paradigm.

    Bustin, Stephen / Kirvell, Sara / Huggett, Jim F / Nolan, Tania

    International journal of molecular sciences

    2021  Volume 22, Issue 16

    Abstract: The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an established tool for the diagnosis of RNA pathogens. Its potential for automation has caused it to be used as a presence/absence diagnostic tool even when RNA quantification ...

    Abstract The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an established tool for the diagnosis of RNA pathogens. Its potential for automation has caused it to be used as a presence/absence diagnostic tool even when RNA quantification is not required. This technology has been pushed to the forefront of public awareness by the COVID-19 pandemic, as its global application has enabled rapid and analytically sensitive mass testing, with the first assays targeting three viral genes published within days of the publication of the SARS-CoV-2 genomic sequence. One of those, targeting the RNA-dependent RNA polymerase gene, has been heavily criticised for supposed scientific flaws at the molecular and methodological level, and this criticism has been extrapolated to doubts about the validity of RT-qPCR for COVID-19 testing in general. We have analysed this assay in detail, and our findings reveal some limitations but also highlight the robustness of the RT-qPCR methodology for SARS-CoV-2 detection. Nevertheless, whilst our data show that some errors can be tolerated, it is always prudent to confirm that the primer and probe sequences complement their intended target, since, when errors do occur, they may result in a reduction in the analytical sensitivity. However, in this case, it is unlikely that a mismatch will result in poor specificity or a significant number of false-positive SARS-CoV-2 diagnoses, especially as this is routinely checked by diagnostic laboratories as part of their quality assurance.
    MeSH term(s) COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/virology ; COVID-19 Testing/methods ; Clinical Laboratory Techniques/methods ; Humans ; Molecular Diagnostic Techniques/methods ; Pandemics ; RNA, Viral/genetics ; RNA-Dependent RNA Polymerase ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Temperature
    Chemical Substances RNA, Viral ; RNA-Dependent RNA Polymerase (EC 2.7.7.48)
    Language English
    Publishing date 2021-08-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22168702
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Monkeypox: another test for PCR.

    Huggett, Jim F / French, David / O'Sullivan, Denise M / Moran-Gilad, Jacob / Zumla, Alimuddin

    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

    2022  Volume 27, Issue 32

    Abstract: Monkeypox was declared a public health emergency of international concern by the World Health Organization (WHO) on 23 July 2022. Between 1 January and 23 July 2022, 16,016 laboratory confirmed cases of monkeypox and five deaths were reported to WHO from ...

    Abstract Monkeypox was declared a public health emergency of international concern by the World Health Organization (WHO) on 23 July 2022. Between 1 January and 23 July 2022, 16,016 laboratory confirmed cases of monkeypox and five deaths were reported to WHO from 75 countries on all continents. Public health authorities are proactively identifying cases and tracing their contacts to contain its spread. As with COVID-19, PCR is the only method capable of being deployed at sufficient speed to provide timely feedback on any public health interventions. However, at this point, there is little information on how those PCR assays are being standardised between laboratories. A likely reason is that testing is still limited on a global scale and that detection, not quantification, of monkeypox virus DNA is the main clinical requirement. Yet we should not be complacent about PCR performance. As testing requirements increase rapidly and specimens become more diverse, it would be prudent to ensure PCR accuracy from the outset to support harmonisation and ease regulatory conformance. Lessons from COVID-19 should aid implementation with appropriate material, documentary and methodological standards offering dynamic mechanisms to ensure testing that most accurately guides public health decisions.
    MeSH term(s) COVID-19 ; COVID-19 Testing ; Humans ; Mpox (monkeypox)/diagnosis ; Mpox (monkeypox)/epidemiology ; Monkeypox virus/genetics ; Polymerase Chain Reaction/methods ; World Health Organization
    Language English
    Publishing date 2022-07-31
    Publishing country Sweden
    Document type Journal Article
    ZDB-ID 1338803-4
    ISSN 1560-7917 ; 1025-496X
    ISSN (online) 1560-7917
    ISSN 1025-496X
    DOI 10.2807/1560-7917.ES.2022.27.32.2200497
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5066: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Reproducibility of biomedical research - The importance of editorial vigilance.

    Bustin, Stephen A / Huggett, Jim F

    Biomolecular detection and quantification

    2017  Volume 11, Page(s) 1–3

    Abstract: Many journal editors are a failing to implement their own authors' instructions, resulting in the publication of many articles that do not meet basic standards of transparency, employ unsuitable data analysis methods and report overly optimistic ... ...

    Abstract Many journal editors are a failing to implement their own authors' instructions, resulting in the publication of many articles that do not meet basic standards of transparency, employ unsuitable data analysis methods and report overly optimistic conclusions. This problem is particularly acute where quantitative measurements are made and results in the publication of papers that lack scientific rigor and contributes to the concerns with regard to the reproducibility of biomedical research. This hampers research areas such as biomarker identification, as reproducing all but the most striking changes is challenging and translation to patient care rare.
    Language English
    Publishing date 2017-02-21
    Publishing country Germany
    Document type Editorial
    ZDB-ID 2821770-6
    ISSN 2214-7535
    ISSN 2214-7535
    DOI 10.1016/j.bdq.2017.01.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: qPCR, dPCR, NGS – A journey

    Jim F. Huggett

    Biomolecular Detection and Quantification, Vol 3, Iss C, Pp A1-A

    2015  Volume 5

    Keywords qPCR ; Digital PCR ; Reverse transcription ; MIQE ; Next generation sequencing ; Biology (General) ; QH301-705.5 ; Microbiology ; QR1-502
    Language English
    Publishing date 2015-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Distribution of Tetrodotoxin in Pacific Oysters (

    Dhanji-Rapkova, Monika / Turner, Andrew D / Baker-Austin, Craig / Huggett, Jim F / Ritchie, Jennifer M

    Marine drugs

    2021  Volume 19, Issue 2

    Abstract: A potent and heat-stable tetrodotoxin (TTX) has been found to accumulate in various marine bivalve species, including Pacific oysters ( ...

    Abstract A potent and heat-stable tetrodotoxin (TTX) has been found to accumulate in various marine bivalve species, including Pacific oysters (
    MeSH term(s) Animals ; Crassostrea/chemistry ; Gastrointestinal Tract/chemistry ; Gills/chemistry ; Poisons/analysis ; Tandem Mass Spectrometry/methods ; Tetrodotoxin/analysis ; Tissue Distribution/physiology ; Water Pollutants, Chemical/analysis
    Chemical Substances Poisons ; Water Pollutants, Chemical ; Tetrodotoxin (4368-28-9)
    Language English
    Publishing date 2021-02-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19020084
    Database MEDical Literature Analysis and Retrieval System OnLINE

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