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  1. Book ; Online ; Thesis: Epitheliale und endotheliale Effekte der Caveolin-1-Deletion in der Niere

    Willière, Yan [Verfasser]

    2019  

    Author's details Yan Willière
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Medizinische Fakultät Charité - Universitätsmedizin Berlin
    Publishing place Berlin
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Article ; Online: Caveolin 1 Promotes Renal Water and Salt Reabsorption.

    Willière, Yan / Borschewski, Aljona / Patzak, Andreas / Nikitina, Tatiana / Dittmayer, Carsten / Daigeler, Anna L / Schuelke, Markus / Bachmann, Sebastian / Mutig, Kerim

    Scientific reports

    2018  Volume 8, Issue 1, Page(s) 545

    Abstract: Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1-/-) mice ... ...

    Abstract Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1-/-) mice were studied. Cav1 expression and caveolae formation were present in vascular cells, late distal convoluted tubule and principal connecting tubule and collecting duct cells of WT but not Cav1-/- kidneys. Urinary sodium excretion was increased by 94% and urine flow by 126% in Cav1-/- mice (p < 0.05). A decrease in activating phosphorylation of the Na-Cl cotransporter (NCC) of the distal convoluted tubule was recorded in Cav1-/- compared to WT kidneys (-40%; p < 0.05). Isolated intrarenal arteries from Cav1-/- mice revealed a fourfold reduction in sensitivity to phenylephrine (p < 0.05). A significantly diminished maximal contractile response (-13%; p < 0.05) was suggestive of enhanced nitric oxide (NO) availability. In line with this, the abundance of endothelial NO synthase (eNOS) was increased in Cav1-/- kidneys +213%; p < 0.05) and cultured caveolae-deprived cells showed intracellular accumulation of eNOS, compared to caveolae-intact controls. Our results suggest that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation of vascular eNOS.
    MeSH term(s) Animals ; Caveolin 1/genetics ; Caveolin 1/metabolism ; Cells, Cultured ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/physiology ; Humans ; Kidney/blood supply ; Kidney/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type III/metabolism ; Renal Artery/metabolism ; Renal Artery/physiology ; Renal Reabsorption ; Sodium/metabolism ; Sodium-Calcium Exchanger/metabolism
    Chemical Substances Cav1 protein, mouse ; Caveolin 1 ; Sodium-Calcium Exchanger ; Nitric Oxide (31C4KY9ESH) ; Sodium (9NEZ333N27) ; Nitric Oxide Synthase Type III (EC 1.14.13.39)
    Language English
    Publishing date 2018-01-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-19071-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Caveolin 1 Promotes Renal Water and Salt Reabsorption

    Yan Willière / Aljona Borschewski / Andreas Patzak / Tatiana Nikitina / Carsten Dittmayer / Anna L. Daigeler / Markus Schuelke / Sebastian Bachmann / Kerim Mutig

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 14

    Abstract: Abstract Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/− ...

    Abstract Abstract Caveolin-1 (Cav1) is essential for the formation of caveolae. Little is known about their functional role in the kidney. We tested the hypothesis that caveolae modulate renal salt and water reabsorption. Wild-type (WT) and Cav1-deficient (Cav1−/−) mice were studied. Cav1 expression and caveolae formation were present in vascular cells, late distal convoluted tubule and principal connecting tubule and collecting duct cells of WT but not Cav1−/− kidneys. Urinary sodium excretion was increased by 94% and urine flow by 126% in Cav1−/− mice (p < 0.05). A decrease in activating phosphorylation of the Na-Cl cotransporter (NCC) of the distal convoluted tubule was recorded in Cav1−/− compared to WT kidneys (−40%; p < 0.05). Isolated intrarenal arteries from Cav1−/− mice revealed a fourfold reduction in sensitivity to phenylephrine (p < 0.05). A significantly diminished maximal contractile response (−13%; p < 0.05) was suggestive of enhanced nitric oxide (NO) availability. In line with this, the abundance of endothelial NO synthase (eNOS) was increased in Cav1−/− kidneys +213%; p < 0.05) and cultured caveolae-deprived cells showed intracellular accumulation of eNOS, compared to caveolae-intact controls. Our results suggest that renal caveolae help to conserve water and electrolytes via modulation of NCC function and regulation of vascular eNOS.
    Keywords Medicine ; R ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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