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Article ; Online: Electrically modulated attachment and detachment of animal cells cultured on an optically transparent patterning electrode.

Koyama, Sumihiro

Journal of bioscience and bioengineering

2011 Volume 111, Issue 5, Page(s) 574–583

Abstract: The purpose of this study was to develop the modulation methods for the attachment and detachment of specifically positioned adhesive animal cells cultured on an electrode surface with the application of a weak electrical potential. A patterned indium ... ...

Abstract	The purpose of this study was to develop the modulation methods for the attachment and detachment of specifically positioned adhesive animal cells cultured on an electrode surface with the application of a weak electrical potential. A patterned indium tin oxide (ITO) optically transparent working electrode was placed on the bottom of a chamber slide with a counter-(Pt) and reference (Ag/AgCl) electrode. The ITO patterning was formed by a reticulate ITO region and arrayed square glass regions of varying size. Using the 3-electrode culture system, the author succeeded in modulation of the attachment and detachment of animal cells on the working electrode surface. Animal cells suspended in serum or sera containing medium were drawn to and attached on a reticulate ITO electrode region to which a +0.4-V vs. Ag/AgCl-positive potential was applied. Meanwhile, the cells were successfully placed on the square glass regions by -0.3-V vs. Ag/AgCl-negative potential application. Animal cells were detached not only from the ITO electrode but also from the square glass regions after the application of a ±10-mV vs. Ag/AgCl, 9-MHz [corrected] rectangular wave potential in PBS(-) for 30-60 min. Rectangular wave potential-induced cell detachment is almost completely noncytotoxic, and no statistical differences between trypsinization and the high frequency wave potential application were observed in HeLa cell growth. The electrical modulation of the specifically positioned cell attachment and detachment techniques holds potential for novel optical microscopic cell sorting analysis in lab-on-chip devices.
MeSH term(s)	Animals ; Cell Adhesion ; Cell Culture Techniques ; Cell Survival ; Collagen/chemistry ; Culture Media ; Eels ; Electricity ; Extracellular Matrix Proteins/chemistry ; Fibroblasts/cytology ; Fluorescein-5-isothiocyanate/chemistry ; Glass ; HeLa Cells ; Humans ; Microelectrodes ; PC12 Cells ; Polylysine/chemistry ; Rats ; Tin Compounds/chemistry
Chemical Substances	Culture Media ; Extracellular Matrix Proteins ; Tin Compounds ; Polylysine (25104-18-1) ; indium tin oxide (71243-84-0) ; Collagen (9007-34-5) ; Fluorescein-5-isothiocyanate (I223NX31W9)
Language	English
Publishing date	2011-05
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1465387-4
ISSN	1347-4421 ; 1389-1723
ISSN (online)	1347-4421
ISSN	1389-1723
DOI	10.1016/j.jbiosc.2010.12.027
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Article: Alternating electric field application induced non-contact and enzyme-free cell detachment.

Koyama, Sumihiro / Wada, Masanori / Tamura, Yasuyuki / Ishikawa, Gen / Kobayashi, Junji / Ishikawa, Yoichi

Cytotechnology

2019 Volume 71, Issue 2, Page(s) 583–597

Abstract: Low intensity (< 2 ... ...

Abstract	Low intensity (< 2 V
Language	English
Publishing date	2019-02-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	1035772-5
ISSN	0920-9069
ISSN	0920-9069
DOI	10.1007/s10616-019-00307-4
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Article: Acceleration of germination and early growth of plant seeds by high frequency and low intensity alternating electric fields

Koyama, Sumihiro / Ishikawa, Gen / Ishikawa, Yoichi / Tamura, Yasuyuki

Engineering in agriculture, environment and food. 2019 Dec. 09,

2019

Abstract: The purpose of this study was to accelerate germination of plant seeds with application of a high frequency alternating electric field. In the present study we show that low-intensity (0.05–1 Vpp/cm) and high frequency (10–100 MHz) alternating electric ... ...

Abstract	The purpose of this study was to accelerate germination of plant seeds with application of a high frequency alternating electric field. In the present study we show that low-intensity (0.05–1 Vpp/cm) and high frequency (10–100 MHz) alternating electric fields accelerate seed germination. We found that 1 Vpp/cm (peak to peak voltage/cm), 100 MHz alternating electric field application for 10 h significantly shortened germination time of arugula, Japanese mustard spinach, and pea seeds, respectively. In arugula and Japanese mustard spinach seeds at 24 h incubation and pea seeds at 32 h incubation after beginning of the application, the germination rates of the electric field applied plant seeds were enhanced 1.5–2.2 times compared to the untreated seeds, respectively. After 72 h of the incubation, the 1 Vpp/cm, 100 MHz alternating electric field for 10 h significantly increased 1.4 folds of culm lengths in the arugula, the Japanese mustard spinach, and the pea seeds compared with those of the untreated seeds.
Keywords	arugula ; culms ; electric field ; electric potential difference ; peas ; seed germination ; seeds ; spinach
Language	English
Dates of publication	2019-1209
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	2668470-6
ISSN	1881-8366
ISSN	1881-8366
DOI	10.1016/j.eaef.2019.12.006
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Article ; Online: Planktotalealamellibrachiae sp. nov., isolated from a marine organism in Kagoshima Bay, Japan.

Nogi, Yuichi / Nishi, Shinro / Koyama, Sumihiro

International journal of systematic and evolutionary microbiology

2017 Volume 67, Issue 11, Page(s) 4785–4789

Abstract: A novel marine bacterial strain, designated JAM ... ...

Abstract	A novel marine bacterial strain, designated JAM 119
MeSH term(s)	Aquatic Organisms/microbiology ; Bacterial Typing Techniques ; Base Composition ; Bays ; DNA, Bacterial/genetics ; Fatty Acids/chemistry ; Japan ; Phospholipids/chemistry ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Rhodobacteraceae/classification ; Rhodobacteraceae/genetics ; Rhodobacteraceae/isolation & purification ; Sequence Analysis, DNA ; Ubiquinone/chemistry
Chemical Substances	DNA, Bacterial ; Fatty Acids ; Phospholipids ; RNA, Ribosomal, 16S ; Ubiquinone (1339-63-5) ; Ubiquinone Q2 (I7T5V2W47R)
Language	English
Publishing date	2017-10-06
Publishing country	England
Document type	Journal Article
ZDB-ID	2002336-4
ISSN	1466-5034 ; 1466-5026
ISSN (online)	1466-5034
ISSN	1466-5026
DOI	10.1099/ijsem.0.002375
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Article: Cell biology of deep-sea multicellular organisms.

Koyama, Sumihiro

Cytotechnology

2007 Volume 55, Issue 2-3, Page(s) 125–133

Abstract: Establishing tissue cultures derived from deep-sea multicellular organisms has been extremely difficult because of the serious damage they sustain upon decompression and exposure to the high temperature of surface seawater. We developed a novel pressure- ... ...

Abstract	Establishing tissue cultures derived from deep-sea multicellular organisms has been extremely difficult because of the serious damage they sustain upon decompression and exposure to the high temperature of surface seawater. We developed a novel pressure-stat aquarium system for the study of living deep-sea multicellular organisms under pressure. Using this system, we have succeeded in maintaining a variety of deep-sea multicellular organisms under pressure and atmospheric conditions after gradual, slow decompression. Furthermore, we successfully cultivated and freeze-stocked pectoral fin cells of the deep-sea eel Simenchelys parasiticus collected at a depth of 1,162 m under atmospheric pressure conditions. This review describes novel capture and maintenance devices for deep-sea organisms and cell culture studies of the organisms under atmospheric and pressure conditions.
Language	English
Publishing date	2007-11-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	1035772-5
ISSN	0920-9069
ISSN	0920-9069
DOI	10.1007/s10616-007-9110-3
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Article: Determination of extremely high pressure tolerance of brine shrimp larvae by using a new pressure chamber system.

Seo, Mihye / Koyama, Sumihiro / Toyofuku, Takashi / Kojima, Shigeaki / Watanabe, Hiromi

Zoological science

2013 Volume 30, Issue 11, Page(s) 919–923

Abstract: Hydrostatic pressure is the only one of a range of environmental parameters (water temperature, salinity, light availability, and so on) that increases in proportion with depth. Pressure tolerance is therefore essential to understand the foundation of ... ...

Abstract	Hydrostatic pressure is the only one of a range of environmental parameters (water temperature, salinity, light availability, and so on) that increases in proportion with depth. Pressure tolerance is therefore essential to understand the foundation of populations and current diversity of faunal compositions at various depths. In the present study, we used a newly developed pressure chamber system to examine changes in larval activity of the salt-lake crustacean, Artemia franciscana, in response to a range of hydrostatic pressures. We showed that A. franciscana larvae were able to survive for a short period at pressures of ≤ 60 MPa (approximately equal to the pressure of 6000 m deep). At a pressure of > 20 MPa, larval motor ability was suppressed, but not lost. Meanwhile, at a pressure of > 40 MPa, some of the larval motor ability was lost without recovery after decompression. For all experiments, discordance of movement and timing between right and left appendages, was observed at pressures of > 20 MPa. Our results indicate that the limit of pressure for sustaining active behavior of A. franciscana larvae is ∼20 MPa, whereas the limit of pressure for survival is within the range 30-60 MPa. Thus, members of the genus Artemia possess the ability to resist a higher range of pressures than their natural habitat depth. Our findings demonstrated an example of an organism capable of invading deeper environment in terms of physical pressure tolerance, and indicate the need and importance of pressure study as an experimental method.
MeSH term(s)	Animals ; Artemia/physiology ; Hydrostatic Pressure ; Larva/physiology ; Motor Activity
Language	English
Publishing date	2013-11
Publishing country	Japan
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2038883-4
ISSN	0289-0003
ISSN	0289-0003
DOI	10.2108/zsj.30.919
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Article: Piezotolerance of the cytoskeletal structure in cultured deep-sea fish cells using DNA transfection and protein introduction techniques.

Koyama, Sumihiro / Aizawa, Masuo

Cytotechnology

2007 Volume 56, Issue 1, Page(s) 19–26

Abstract: We used DNA transfection and protein introduction techniques to investigate the pressure tolerance of cytoskeletal structures in pectoral fin cells derived from the deep-sea fish Simenchelys parasiticus (habitat depth, 366-2,630 m). The deep-sea fish ... ...

Abstract	We used DNA transfection and protein introduction techniques to investigate the pressure tolerance of cytoskeletal structures in pectoral fin cells derived from the deep-sea fish Simenchelys parasiticus (habitat depth, 366-2,630 m). The deep-sea fish cells have G418 resistance. The cell number increased until day 6 of cultivation and all cells had died by day 35 when cultured in 35-mm Petri dishes in medium containing G418. Enhanced yellow fluorescent protein-tagged human beta-actin (EYFP-actin) was stably expressed by 1 in 100,000 deep-sea fish cells. Because almost none of the EYFP-actin was incorporated into actin filaments of the cells, we replaced the relatively large EYFP tag with a chemical fluorescent compound and succeeded in incorporating fluorescently labeled rabbit actins into the deep-sea fish actin filaments. Most of the filament structure in the cells with rabbit actin inserted underwent depolymerization when subjected to pressure of 100 MPa for 20 min, in contrast to control cells. There were no differences in the tubulin filament structure between control cells and deep-sea fish cells with fluorescein-labeled bovine tubulin inserted after the application of pressure ranging from 40 to 100 MPa for 20 min.
Language	English
Publishing date	2007-10-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	1035772-5
ISSN	0920-9069
ISSN	0920-9069
DOI	10.1007/s10616-007-9099-7
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Article: Transfer of Cesium and Potassium from Grapes to Wine

Goto-Yamamoto, N. / Koyama, K. / Tsukamoto, K. / Kamigakiuchi, H. / Sumihiro, M. / Okuda, M. / Hashiguchi, T. / Matsumaru, K. / Sekizawa, H. / Shimoi, H.

American journal of enology and viticulture

2014 Volume 65, Issue 1, Page(s) 143–147

Document type	Article
ZDB-ID	407380-0
ISSN	0002-9254
Database	Current Contents Nutrition, Environment, Agriculture

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Article: PKC-dependent IL-6 production and inhibition of IL-8 production by PKC activation in normal human skin fibroblasts under extremely high hydrostatic pressure.

Koyama, Sumihiro / Aizawa, Masuo

Extremophiles : life under extreme conditions

2002 Volume 6, Issue 5, Page(s) 413–418

Abstract: Normal human dermal fibroblasts were found to survive and to be active in producing interleukin (IL)-6 and IL-8 under extremely high hydrostatic pressure, up to 40 MPa (1 atm=0.101325 MPa=1.03323 kgf/cm(2)), for 20 min. An inhibitor of protein kinase C ( ... ...

Abstract	Normal human dermal fibroblasts were found to survive and to be active in producing interleukin (IL)-6 and IL-8 under extremely high hydrostatic pressure, up to 40 MPa (1 atm=0.101325 MPa=1.03323 kgf/cm(2)), for 20 min. An inhibitor of protein kinase C (PKC) reduced the amount of IL-6 production, whereas IL-8 production was increased following pressure application. The activation of PKC in response to exposure to the pressure stress was detected by using the PKC-specific probe Rim-1. These findings indicate that IL-6 production induced by hydrostatic pressure stresses was dependent on the PKC signaling pathway. In contrast, pressure-induced IL-8 production was inhibited by PKC activity.
MeSH term(s)	Catalytic Domain/drug effects ; Cells, Cultured/metabolism ; Enzyme Inhibitors/pharmacology ; Fibroblasts/metabolism ; Fluorescent Dyes/pharmacology ; Gene Expression Regulation/drug effects ; Humans ; Hydrostatic Pressure ; Indoles/pharmacology ; Interleukin-1/analysis ; Interleukin-15/analysis ; Interleukin-6/biosynthesis ; Interleukin-6/genetics ; Interleukin-8/biosynthesis ; Interleukin-8/genetics ; Maleimides/pharmacology ; Protein Kinase C/antagonists & inhibitors ; Protein Kinase C/pharmacology ; Rhodamines/pharmacology ; Skin/cytology ; Subcellular Fractions/enzymology
Chemical Substances	Enzyme Inhibitors ; Fluorescent Dyes ; Indoles ; Interleukin-1 ; Interleukin-15 ; Interleukin-6 ; Interleukin-8 ; Maleimides ; Rhodamines ; rim 1 (150206-04-5) ; Protein Kinase C (EC 2.7.11.13) ; bisindolylmaleimide I (L79H6N0V6C)
Language	English
Publishing date	2002-10
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1481278-2
ISSN	1433-4909 ; 1431-0651
ISSN (online)	1433-4909
ISSN	1431-0651
DOI	10.1007/s00792-002-0273-8
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Article ; Online: Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment.

Tsubouchi, Taishi / Koyama, Sumihiro / Mori, Kozue / Shimane, Yasuhiro / Usui, Keiko / Tokuda, Maki / Tame, Akihiro / Uematsu, Katsuyuki / Maruyama, Tadashi / Hatada, Yuji

International journal of systematic and evolutionary microbiology

2014 Volume 64, Issue Pt 11, Page(s) 3709–3716

Abstract: A novel Gram-stain-negative, aerobic, heterotrophic, stalked and capsulated bacterium with potential denitrification ability, designated strain TAR-002(T), was isolated from deep seafloor sediment in Japan. Colonies lacked lustre, and were viscous and ... ...

Abstract	A novel Gram-stain-negative, aerobic, heterotrophic, stalked and capsulated bacterium with potential denitrification ability, designated strain TAR-002(T), was isolated from deep seafloor sediment in Japan. Colonies lacked lustre, and were viscous and translucent white. The ranges of temperature, pH and salt concentration for growth were 8-30 °C, pH 6.0-10.0 and 1-3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-002(T) belongs to the genus Brevundimonas of the class Alphaproteobacteria. Levels of similarity between the 16S rRNA gene sequence of strain TAR-002(T) and those of the type strains of species of the genus Brevundimonas were 93.5-98.9%; the most closely related species was Brevundimonas basaltis. In DNA-DNA hybridization assays between strain TAR-002(T) and its phylogenetic neighbours, Brevundimonas lenta DS-18(T), B. basaltis J22(T), Brevundimonas subvibrioides ATCC 15264(T) and Brevundimonas alba DSM 4736(T), mean hybridization levels were 6.4-27.7%. The G+C content of strain TAR-002(T) was 70.3 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were C(18:1)ω7c and C(16:0), and the presence of 1,2-di-O-acyl-3-O-[D-glucopyranosyl-(1 → 4)-α-D-glucopyranuronosyl]glycerol (DGL) indicates the affiliation of strain TAR-002(T) with the genus Brevundimonas. On the basis of biological characteristics and 16S rRNA gene sequence comparisons, strain TAR-002(T) is considered to represent a novel species of the genus Brevundimonas, for which the name Brevundimonas denitrificans sp. nov. is proposed; the type strain is TAR-002(T) ( =NBRC 110107(T) =CECT 8537(T)).
MeSH term(s)	Bacterial Typing Techniques ; Base Composition ; Caulobacteraceae/classification ; Caulobacteraceae/genetics ; Caulobacteraceae/isolation & purification ; DNA, Bacterial/genetics ; Denitrification ; Fatty Acids/chemistry ; Geologic Sediments/microbiology ; Japan ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Seawater/microbiology ; Sequence Analysis, DNA ; Ubiquinone/chemistry
Chemical Substances	DNA, Bacterial ; Fatty Acids ; RNA, Ribosomal, 16S ; Ubiquinone (1339-63-5) ; Ubiquinone Q2 (I7T5V2W47R)
Language	English
Publishing date	2014-08-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2002336-4
ISSN	1466-5034 ; 1466-5026
ISSN (online)	1466-5034
ISSN	1466-5026
DOI	10.1099/ijs.0.067199-0
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