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Article ; Online: Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence.

Kesoju, Sandya R / Kramer, Matthew / Brunet, Johanne / Greene, Stephanie L / Jordan, Amelia / Martin, Ruth C

2021 Volume 16, Issue 3, Page(s) e0248746

Abstract: In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between ... ...

Abstract	In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between genetically engineered (GE) and conventional fields on adventitious presence (AP) which represents the unwanted presence of a GE gene. Variables other than distance, however, may affect AP. In addition, some AP is often present in the parent seed lots used to establish conventional fields. To identify variables that influence the proportion of AP in conventional alfalfa fields, we performed variable selection regression analyses. Analyses based on a sample-level and a field-level analysis gave similar, though not identical results. For the sample-level model, distance from the GE field explained 66% of the variance in AP, confirming its importance in affecting AP. The area of GE fields within the pollinator foraging range explained an additional 30% of the variation in AP in the model. The density of alfalfa leafcutting bee domiciles influenced AP in both models. To minimize AP in conventional alfalfa seed fields, management practices should focus on optimizing isolation distances while also considering the size of the GE pollen pool within the pollinator foraging range, and the foraging behavior of pollinators.
MeSH term(s)	Animals ; Crops, Agricultural/genetics ; Gene Flow ; Insecta/physiology ; Medicago sativa/genetics ; Plants, Genetically Modified/genetics ; Pollination ; Washington
Language	English
Publishing date	2021-03-25
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0248746
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Profitability of cotton, Gossypium hirsutum (L.), when controlling bollworm, Helicoverpa zea (Boddie), with variable treatment thresholds

Calvin, Wilfrid / Yang, Fei / Gore, Jeffrey / Greene, Jeremy K. / Kerns, David L.

Crop Protection. 2023 Nov., v. 173 p.106368-

2023

Abstract: Crops expressing Bacillus thuringiensis proteins have offered valuable crop protection benefits including reduced foliar insecticide use and crop yield protection. Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) has developed resistance to one or more ... ...

Abstract	Crops expressing Bacillus thuringiensis proteins have offered valuable crop protection benefits including reduced foliar insecticide use and crop yield protection. Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) has developed resistance to one or more Bt proteins, which consequently threatens these benefits. This has resulted in a renewed emphasis on the implementation of action thresholds for H. zea management decisions in cotton. Field experiments were conducted in College Station, TX; Stoneville, MS; and Blackville, SC, during 2020–2021 to evaluate current H. zea threshold recommendations for Texas, Mississippi, and South Carolina. Three Bt cotton technologies and five thresholds were evaluated. The Bt cotton technologies evaluated included non-Bt (NBT), Bollgard® 2 (BG2; Cry1Ac + Cry2Ab), and Bollgard® 3 (BG3; Cry1Ac + Cry2Ab + Vip3Aa). Across years and locations, within NBT cotton, all thresholds, including 20% oviposition, 6% fruiting forms injury, ≥3% large larvae, and preventive sprays, resulted in greater control of H. zea and higher yield and profitability relative to non-treated NBT cotton. The threshold using 6% injury to squares and bolls was the most effective in terms of yield relative to the preventive spray. Across years and locations, the BG2 cotton sprayed based on 20% oviposition or preventively resulted in greater control of H. zea, and all treatments resulted in a yield or profitability equivalent to that of the non-treated BG2 cotton. For BG3 cotton, spraying based on any of the thresholds was not beneficial. For NBT and BG2 cotton, thresholds of 20% oviposition, 6% injury to fruiting forms, or ≥3% large larvae provided a reduction in insecticide applications relative to the preventive spray treatment, suggesting that these thresholds were effective triggers for controlling H. zea.
Keywords	Bacillus thuringiensis ; Gossypium hirsutum ; Helicoverpa zea ; cotton ; crop yield ; insecticides ; oviposition ; plant protection ; profitability ; Mississippi ; South Carolina ; Texas ; Action threshold ; Chlorantraniliprole ; IPM
Language	English
Dates of publication	2023-11
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	786839-x
ISSN	1873-6904 ; 0261-2194
ISSN (online)	1873-6904
ISSN	0261-2194
DOI	10.1016/j.cropro.2023.106368
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Distributions, conservation status, and abiotic stress tolerance potential of wild cucurbits (Cucurbita L.)

Colin K. Khoury / Daniel Carver / Heather R. Kates / Harold A. Achicanoy / Maarten vanZonneveld / Evert Thomas / Claire Heinitz / Robert Jarret / Joanne A. Labate / Kathy Reitsma / Gary P. Nabhan / Stephanie L. Greene

Plants, People, Planet, Vol 2, Iss 3, Pp 269-

2020 Volume 283

Abstract: ... Cucurbita L.). The taxa occur from the central USA to Central America, plus two South American species ... Here we model the potential ranges of all 16 known wild cucurbit taxa (Cucurbita L.), use ecogeographic ...

Abstract	Societal Impact Statement Crop wild relatives—wild species closely related to cultivated plants—are valuable genetic resources for crop improvement, but gaps in knowledge constrain their conservation and limit their further use. We develop new information on the distributions, potential breeding value, and conservation status of the 16 known wild relatives of cultivated pumpkins, squashes, zucchini, and gourds (Cucurbita L.). The taxa occur from the central USA to Central America, plus two South American species, with the greatest richness in central Mexico and the western borderlands between Mexico and the USA. We determine the majority of species are of medium priority for conservation, both with regard to collecting for ex situ maintenance, and for enhanced habitat protection. Summary Crop wild relatives are valuable genetic resources for crop improvement. Knowledge gaps, including with regard to taxonomy, distributions, and characterization for traits of interest constrain their use in plant breeding. These deficiencies also affect conservation planning, both with regard to in situ habitat protection, and further collection of novel diversity for ex situ maintenance. Here we model the potential ranges of all 16 known wild cucurbit taxa (Cucurbita L.), use ecogeographic information to infer their potential adaptations to abiotic stresses, and assess their ex situ and in situ conservation status. The taxa occur from the central USA to Central America, plus two South American species. Predicted taxon richness was highest in central Mexico and in the western borderlands between Mexico and the USA. We find substantial ecogeographic variation both across taxa and among populations within taxa, with regard to low temperatures, high and low precipitation, and other adaptations of potential interest for crop breeding. We categorize 13 of the taxa medium priority for further conservation as a combination of the ex situ and in situ assessments, two low priority, and one sufficiently conserved. Further action across the ...
Keywords	biodiversity conservation ; crop wild relatives ; Cucurbita ; ex situ conservation ; gap analysis ; in situ conservation ; Environmental sciences ; GE1-350 ; Botany ; QK1-989
Subject code	333
Language	English
Publishing date	2020-05-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Distributions, conservation status, and abiotic stress tolerance potential of wild cucurbits (Cucurbita L.)

Khoury, Colin K. / Carver, Daniel / Kates, Heather R. / Achicanoy, Harold A. / van Zonneveld, Maarten / Thomas, Evert / Heinitz, Claire / Jarret, Robert / Labate, Joanne A. / Reitsma, Kathy / Nabhan, Gary P. / Greene, Stephanie L.

Plants, people, planet. 2020 May, v. 2, no. 3

2020

Abstract: ... status of the 16 known wild relatives of cultivated pumpkins, squashes, zucchini, and gourds (Cucurbita L ... Here we model the potential ranges of all 16 known wild cucurbit taxa (Cucurbita L.), use ecogeographic ...

Abstract	Crop wild relatives—wild species closely related to cultivated plants—are valuable genetic resources for crop improvement, but gaps in knowledge constrain their conservation and limit their further use. We develop new information on the distributions, potential breeding value, and conservation status of the 16 known wild relatives of cultivated pumpkins, squashes, zucchini, and gourds (Cucurbita L.). The taxa occur from the central USA to Central America, plus two South American species, with the greatest richness in central Mexico and the western borderlands between Mexico and the USA. We determine the majority of species are of medium priority for conservation, both with regard to collecting for ex situ maintenance, and for enhanced habitat protection. Summary Crop wild relatives are valuable genetic resources for crop improvement. Knowledge gaps, including with regard to taxonomy, distributions, and characterization for traits of interest constrain their use in plant breeding. These deficiencies also affect conservation planning, both with regard to in situ habitat protection, and further collection of novel diversity for ex situ maintenance. Here we model the potential ranges of all 16 known wild cucurbit taxa (Cucurbita L.), use ecogeographic information to infer their potential adaptations to abiotic stresses, and assess their ex situ and in situ conservation status. The taxa occur from the central USA to Central America, plus two South American species. Predicted taxon richness was highest in central Mexico and in the western borderlands between Mexico and the USA. We find substantial ecogeographic variation both across taxa and among populations within taxa, with regard to low temperatures, high and low precipitation, and other adaptations of potential interest for crop breeding. We categorize 13 of the taxa medium priority for further conservation as a combination of the ex situ and in situ assessments, two low priority, and one sufficiently conserved. Further action across the distributions of the taxa, with emphasis on taxonomic richness hotspots, is needed to comprehensively conserve wild Cucurbita populations.
Keywords	Cucurbita ; abiotic stress ; conservation status ; habitat conservation ; stress tolerance ; taxonomy ; zucchini ; Central America ; Mexico ; Midwestern United States
Language	English
Dates of publication	2020-05
Size	p. 269-283.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ISSN	2572-2611
DOI	10.1002/ppp3.10085
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Distributions, conservation status, and abiotic stress tolerance potential of wild cucurbits (Cucurbita L.)

Khoury, Colin K. / Carver, Daniel / Kates, Heather R. / Achicanoy, Harold A.E. / Zonneveld, Maarten van / Thomas, Evert / Heinitz, Claire / Jarret, Robert L. / Labate, Joanne A. / Reitsma, Kathy / Nabhan, Gary P. / Greene, Stephanie L.

Plant People Planet

2019

Abstract: ... ranges of all 16 known wild cucurbit taxa (Cucurbita L.), use ecogeographic information to infer ...

Abstract	Crop wild relatives are valuable genetic resources for crop improvement. Knowledge gaps, including with regard to taxonomy, distributions, and characterization for traits of interest constrain their use in plant breeding. These deficiencies also affect conservation planning, both with regard to in situ habitat protection, and further collection of novel diversity for ex situ maintenance. Here we model the potential ranges of all 16 known wild cucurbit taxa (Cucurbita L.), use ecogeographic information to infer their potential adaptations to abiotic stresses, and assess their ex situ and in situ conservation status. The taxa occur from the central USA to Central America, plus two South American species. Predicted taxon richness was highest in central Mexico and in the western borderlands between Mexico and the USA. We find substantial ecogeographic variation both across taxa and among populations within taxa, with regard to low temperatures, high and low precipitation, and other adaptations of potential interest for crop breeding. We categorize 13 of the taxa medium priority for further conservation as a combination of the ex situ and in situ assessments, two low priority, and one sufficiently conserved. Further action across the distributions of the taxa, with emphasis on taxonomic richness hotspots, is needed to comprehensively conserve wild Cucurbita populations.
Keywords	crop wild ; crop improvement ; plant breeding ; conservation ; taxonomy ; ex-situ conservation ; abiotic stress ; tolerance
Language	English
Publishing date	2019-12-11T15:10:46Z
Publisher	Wiley
Publishing country	fr
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Injectable Poly-L-Lactic Acid: Understanding Its Use in the Current Era.

Lorenc, Z Paul / Greene, Thomas / Gottschalk, Ronald W

Journal of drugs in dermatology : JDD

2016 Volume 15, Issue 6, Page(s) 759–762

Abstract: A survey of Sculptra ... ® ... Aesthetic injectors was conducted to understand how the product is being currently reconstituted and injected. Questions were asked of injectors to understand their reasons for choice and volume of diluent(s), additions, ... ...

Abstract	A survey of Sculptra<sup>®</sup> Aesthetic injectors was conducted to understand how the product is being currently reconstituted and injected. Questions were asked of injectors to understand their reasons for choice and volume of diluent(s), additions, and time for the reconstitution process. These results are discussed in the context of the past history of the product over the last decade, with a focus on adverse events such as papules and nodules. <em>J Drugs Dermatol. </em>2016;15(6):759-762.
MeSH term(s)	Cellulose/administration & dosage ; Cosmetic Techniques/trends ; Humans ; Injections, Subcutaneous ; Lactic Acid/administration & dosage ; Mannitol/administration & dosage ; Polyesters/administration & dosage ; Skin Aging/drug effects ; Skin Aging/pathology ; Surveys and Questionnaires
Chemical Substances	New-Fill ; Polyesters ; Lactic Acid (33X04XA5AT) ; Mannitol (3OWL53L36A) ; poly(lactide) (459TN2L5F5) ; Cellulose (9004-34-6)
Language	English
Publishing date	2016-06-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2145090-0
ISSN	1545-9616
ISSN	1545-9616
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields

Sandya R Kesoju / Matthew Kramer / Johanne Brunet / Stephanie L Greene / Amelia Jordan / Ruth C Martin

PLoS ONE, Vol 16, Iss 3, p e

Distance is the primary but not the sole influence on adventitious presence.

2021 Volume 0248746

Abstract	In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between genetically engineered (GE) and conventional fields on adventitious presence (AP) which represents the unwanted presence of a GE gene. Variables other than distance, however, may affect AP. In addition, some AP is often present in the parent seed lots used to establish conventional fields. To identify variables that influence the proportion of AP in conventional alfalfa fields, we performed variable selection regression analyses. Analyses based on a sample-level and a field-level analysis gave similar, though not identical results. For the sample-level model, distance from the GE field explained 66% of the variance in AP, confirming its importance in affecting AP. The area of GE fields within the pollinator foraging range explained an additional 30% of the variation in AP in the model. The density of alfalfa leafcutting bee domiciles influenced AP in both models. To minimize AP in conventional alfalfa seed fields, management practices should focus on optimizing isolation distances while also considering the size of the GE pollen pool within the pollinator foraging range, and the foraging behavior of pollinators.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Relationship Between Piercing-Sucking Insect Control and Internal Lint and Seed Rot in Southeastern Cotton (Gossypium hirsutum L.).

Medrano, Enrique G / Bell, Alois A / Greene, Jeremy K / Roberts, Phillip M / Bacheler, Jack S / Marois, James J / Wright, David L / Esquivel, Jesus F / Nichols, Robert L / Duke, Sara

Journal of economic entomology

2015 Volume 108, Issue 4, Page(s) 1540–1544

Abstract: ... bacterium Pantoea agglomerans by the southern green stink bug, Nezara viridula (L.). Here, green bolls were ...

Abstract	In 1999, crop consultants scouting for stink bugs (Hemiptera spp.) in South Carolina discovered a formerly unobserved seed rot of cotton that caused yield losses ranging from 10 to 15% in certain fields. The disease has subsequently been reported in fields throughout the southeastern Cotton Belt. Externally, diseased bolls appeared undamaged; internally, green fruit contain pink to dark brown, damp, deformed lint, and necrotic seeds. In greenhouse experiments, we demonstrated transmission of the opportunistic bacterium Pantoea agglomerans by the southern green stink bug, Nezara viridula (L.). Here, green bolls were sampled from stink bug management plots (insecticide protected or nontreated) from four South Atlantic coast states (North Carolina, South Carolina, Georgia, and Florida) to determine disease incidence in the field and its association with piercing-sucking insects feeding. A logistic regression analysis of the boll damage data revealed that disease was 24 times more likely to occur (P = 0.004) in bolls collected from plots in Florida, where evidence of pest pressure was highest, than in bolls harvested in NC with the lowest detected insect pressure. Fruit from plots treated with insecticide, a treatment which reduced transmission agent numbers, were 4 times less likely to be diseased than bolls from unprotected sites (P = 0.002). Overall, punctured bolls were 125 times more likely to also have disease symptoms than nonpunctured bolls, irrespective of whether or not plots were protected with insecticides (P = 0.0001). Much of the damage to cotton bolls that is commonly attributed to stink bug feeding is likely the resulting effect of vectored pathogens.
MeSH term(s)	Animals ; Fruit/microbiology ; Gossypium ; Herbivory ; Heteroptera/physiology ; Insect Control ; Insect Vectors/microbiology ; Models, Biological ; Pantoea/physiology ; Plant Diseases/microbiology ; Seeds/microbiology ; Southeastern United States
Language	English
Publishing date	2015-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3031-4
ISSN	0022-0493
ISSN	0022-0493
DOI	10.1093/jee/tov156
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Zs.B 1118: Show issues

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Article ; Online: Phenotypic changes and DNA methylation status in cryopreserved seeds of rye (Secale cereale L.).

Lu, Jie / Greene, Stephanie / Reid, Scott / Cruz, Von Mark V / Dierig, David A / Byrne, Patrick

Cryobiology

2018 Volume 82, Page(s) 8–14

Abstract: Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed ... ...

Abstract	Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed in the NLGRP: storage in a -18 °C freezer (conventional storage) and storage in liquid nitrogen vapor phase at -135 to -180 °C (cryopreservation). To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. In laboratory evaluations of winter habit rye, seeds from cryopreserved samples had significantly higher normal germination percentage (P < 0.05) and lower abnormal germination percentage (P < 0.05) than those stored under conventional conditions. Cryopreserved spring habit rye also had higher normal germination percentage (P < 0.01) than conventionally stored samples. In addition, winter rye seedlings from cryopreserved seeds had longer roots and smaller root diameter (P < 0.05) than seedlings from conventionally stored seeds. In field evaluations conducted in Fort Collins, Colorado in 2014-15, spikes of plants grown from conventionally stored seeds of the winter accessions were slightly longer than those from cryopreserved seeds (P = 0.045). To detect DNA methylation changes, a methylation-sensitive amplified fragment length polymorphism (metAFLP) technique was applied to two accessions. After false discovery rate adjustment, no differences in methylation were detected between storage treatments on an individual locus basis. Our study indicated that cryopreservation slowed seed deterioration as evidenced by higher germination rates compared to conventional storage, had only minimal effects on other phenotypic traits, and had no significant effects on DNA methylation status.
MeSH term(s)	Amplified Fragment Length Polymorphism Analysis ; Cryopreservation/methods ; DNA Methylation/genetics ; Germination ; Nitrogen/pharmacology ; Phenotype ; Plant Roots/growth & development ; Plants ; Secale/genetics ; Secale/growth & development ; Seedlings/growth & development ; Seeds/growth & development
Chemical Substances	Nitrogen (N762921K75)
Language	English
Publishing date	2018-04-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80098-3
ISSN	1090-2392 ; 0011-2240
ISSN (online)	1090-2392
ISSN	0011-2240
DOI	10.1016/j.cryobiol.2018.04.015
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Article ; Online: Total Analytic Error for Low Cardiac Troponin Concentrations (≤10 ng/L) by Use of a High-Sensitivity Cardiac Troponin Assay.

Kavsak, Peter A / Jaffe, Allan S / Greene, Dina N / Christenson, Robert H / Apple, Fred S / Wu, Alan H B

Clinical chemistry

2017 Volume 63, Issue 5, Page(s) 1043–1045

MeSH term(s)	Biomarkers/blood ; Diagnostic Errors ; Heart Diseases/blood ; Humans ; Limit of Detection ; Troponin/blood
Chemical Substances	Biomarkers ; Troponin
Language	English
Publishing date	2017-03-21
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	80102-1
ISSN	1530-8561 ; 0009-9147
ISSN (online)	1530-8561
ISSN	0009-9147
DOI	10.1373/clinchem.2017.271361
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