Article ; Online: Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP).
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2654, Page(s) 113–122
Abstract: The plasma membrane of cells is covered by proteins, glycoproteins, and glycolipids with molecular heights ranging from just a few nanometers to hundreds of nanometers. Formation of cell-cell contacts and signal transduction by individual receptors can ... ...
Abstract | The plasma membrane of cells is covered by proteins, glycoproteins, and glycolipids with molecular heights ranging from just a few nanometers to hundreds of nanometers. Formation of cell-cell contacts and signal transduction by individual receptors can be dependent on both the average height of a cell's glycocalyx and the specific height of individual receptors, sometimes with nanometer-scale sensitivity. While super-resolution imaging techniques allow molecular distances to be measured with the sub-diffraction limited resolution, typically 10 nm in the lateral direction and 100 nm in the axial direction, measurements of molecular heights at the single nanometer scale on native cell membranes have been difficult to obtain. Cell surface optical profilometry (CSOP) is a simple and rapid method that achieves nanometer height resolution by localizing fluorophores at the tip and base of cell surface molecules and determining their separation with high precision by radially averaging across many molecules. Here we describe how to make CSOP measurements of multi-domain proteins on model membrane surfaces as well as native cell surfaces. |
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MeSH term(s) | Cell Membrane/metabolism ; Glycocalyx ; Glycoproteins/metabolism ; Signal Transduction |
Chemical Substances | Glycoproteins |
Language | English |
Publishing date | 2023-04-27 |
Publishing country | United States |
Document type | Journal Article |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-3135-5_7 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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