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  1. Article ; Online: Systems Analysis of Highly Multiplexed CRISPR-Base Editing in Streptomycetes.

    Whitford, Christopher M / Gren, Tetiana / Palazzotto, Emilia / Lee, Sang Yup / Tong, Yaojun / Weber, Tilmann

    ACS synthetic biology

    2023  Volume 12, Issue 8, Page(s) 2353–2366

    Abstract: CRISPR tools, especially Cas9n-sgRNA guided cytidine deaminase base editors such as CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One major advantage of CRISPR base editing technology is the possibility to multiplex ... ...

    Abstract CRISPR tools, especially Cas9n-sgRNA guided cytidine deaminase base editors such as CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One major advantage of CRISPR base editing technology is the possibility to multiplex experiments in genomically instable species. Here, we demonstrate scaled up Csy4 based multiplexed genome editing using CRISPR-mcBEST in
    MeSH term(s) Gene Editing ; CRISPR-Cas Systems/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; RNA, Guide, CRISPR-Cas Systems ; Actinomycetales/genetics ; Systems Analysis
    Chemical Substances RNA, Guide, CRISPR-Cas Systems
    Language English
    Publishing date 2023-07-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.3c00188
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  2. Article ; Online: Biosynthesis of the Azoxy Compound Azodyrecin from

    Maleckis, Matiss / Wibowo, Mario / Gren, Tetiana / Jarmusch, Scott A / Sterndorff, Eva B / Booth, Thomas / Henriksen, Nathalie N S E / Whitford, Christopher M / Jiang, Xinglin / Jørgensen, Tue S / Ding, Ling / Weber, Tilmann

    ACS chemical biology

    2024  Volume 19, Issue 3, Page(s) 641–653

    Abstract: Azoxy compounds are a distinctive group of bioactive secondary metabolites characterized by a unique RN═ ... ...

    Abstract Azoxy compounds are a distinctive group of bioactive secondary metabolites characterized by a unique RN═N
    MeSH term(s) Phylogeny ; Tandem Mass Spectrometry ; Streptomyces/genetics ; Streptomyces/metabolism ; Multigene Family
    Language English
    Publishing date 2024-02-10
    Publishing country United States
    Document type Journal Article
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.3c00632
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  3. Article ; Online: Impact of face masks on the 6-minute walk distance in pulmonary hypertension patients during the COVID-19 pandemic: a prospective, randomised cross-over study.

    Wissmüller, Max / Wartner, Veit / Hohmann, Christopher / Adler, Joana / Kramer, Tilmann / Hellmich, Martin / Gerhardt, Felix / Baldus, Stephan / Rosenkranz, Stephan

    The European respiratory journal

    2023  Volume 62, Issue 4

    MeSH term(s) Humans ; COVID-19 ; Hypertension, Pulmonary ; Pandemics ; Prospective Studies ; Masks ; Cross-Over Studies
    Language English
    Publishing date 2023-10-26
    Publishing country England
    Document type Randomized Controlled Trial ; Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 639359-7
    ISSN 1399-3003 ; 0903-1936
    ISSN (online) 1399-3003
    ISSN 0903-1936
    DOI 10.1183/13993003.01454-2022
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    In stock of ZB MED Cologne/Königswinter

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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  4. Article ; Online: The Design-Build-Test-Learn cycle for metabolic engineering of Streptomycetes.

    Whitford, Christopher M / Cruz-Morales, Pablo / Keasling, Jay D / Weber, Tilmann

    Essays in biochemistry

    2021  Volume 65, Issue 2, Page(s) 261–275

    Abstract: Streptomycetes are producers of a wide range of specialized metabolites of great medicinal and industrial importance, such as antibiotics, antifungals, or pesticides. Having been the drivers of the golden age of antibiotics in the 1950s and 1960s, ... ...

    Abstract Streptomycetes are producers of a wide range of specialized metabolites of great medicinal and industrial importance, such as antibiotics, antifungals, or pesticides. Having been the drivers of the golden age of antibiotics in the 1950s and 1960s, technological advancements over the last two decades have revealed that very little of their biosynthetic potential has been exploited so far. Given the great need for new antibiotics due to the emerging antimicrobial resistance crisis, as well as the urgent need for sustainable biobased production of complex molecules, there is a great renewed interest in exploring and engineering the biosynthetic potential of streptomycetes. Here, we describe the Design-Build-Test-Learn (DBTL) cycle for metabolic engineering experiments in streptomycetes and how it can be used for the discovery and production of novel specialized metabolites.
    MeSH term(s) Anti-Bacterial Agents ; Metabolic Engineering
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2021-05-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 1744-1358 ; 0071-1365
    ISSN (online) 1744-1358
    ISSN 0071-1365
    DOI 10.1042/EBC20200132
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  5. Article ; Online: A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing

    Yaojun Tong / Tue S. Jørgensen / Christopher M. Whitford / Tilmann Weber / Sang Yup Lee

    Nature Communications, Vol 12, Iss 1, Pp 1-

    2021  Volume 11

    Abstract: CRISPR prime editing enables double-strand break free engineering of the genome. Here the authors present a toolkit for prime editing in E. coli. ...

    Abstract CRISPR prime editing enables double-strand break free engineering of the genome. Here the authors present a toolkit for prime editing in E. coli.
    Keywords Science ; Q
    Language English
    Publishing date 2021-09-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
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  6. Article ; Online: High-Quality Sequencing, Assembly, and Annotation of the Streptomyces griseofuscus DSM 40191 Genome.

    Gren, Tetiana / Jørgensen, Tue S / Whitford, Christopher M / Weber, Tilmann

    Microbiology resource announcements

    2020  Volume 9, Issue 47

    Abstract: Here, we report the sequencing, assembly, and annotation of the genome ... ...

    Abstract Here, we report the sequencing, assembly, and annotation of the genome of
    Language English
    Publishing date 2020-11-19
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.01100-20
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  7. Article ; Online: Impact of rapid sequential combination therapy on distinct haemodynamic measures in newly diagnosed pulmonary arterial hypertension.

    Kramer, Tilmann / Nattmann, Phillip / Gerhardt, Felix / Stafiej, Patrycja / Dumitrescu, Daniel / Ten Freyhaus, Henrik / Wißmüller, Max / Hohmann, Christopher / Baldus, Stephan / Rosenkranz, Stephan

    ESC heart failure

    2024  

    Abstract: Aims: In pulmonary arterial hypertension (PAH), upfront combination therapy with ERA and PDE5i is associated with a reduction in morbidity and mortality events and improves standard haemodynamics, but data remain limited. Aims of this study were (i) to ... ...

    Abstract Aims: In pulmonary arterial hypertension (PAH), upfront combination therapy with ERA and PDE5i is associated with a reduction in morbidity and mortality events and improves standard haemodynamics, but data remain limited. Aims of this study were (i) to capture detailed haemodynamic effects of rapid sequential dual combination therapy in patients with newly diagnosed PAH; (ii) to monitor the impact of treatment initiation on clinical variables and patients' risk status, and (iii) to compare the treatment effect in patients with 'classical PAH' and 'PAH with co-morbidities'.
    Methods: Fifty patients (median age 57 [42-71] years, 66% female) with newly diagnosed PAH (76% idiopathic) were treated with a PD5i/sGC-S or ERA, followed by addition of the respective other drug class within 4 weeks. All patients underwent repeat right heart catheterization (RHC) during early follow-up.
    Results: At early repeat RHC (7 ± 2 months), there were substantial reductions in mean pulmonary artery pressure (mPAP: 52.2 ± 13.5 to 39.0 ± 10.6 mmHg; -25.3%), and pulmonary vascular resistance (PVR: 12.1 ± 5.7 to 5.8 ± 3.1 WU; -52.1%), and an increase in cardiac index (2.1 ± 0.4 to 2.7 ± 0.7 mL/min/m
    Conclusions: Rapid sequential combination therapy with PDE5i/sGC-S and ERA substantially ameliorates cardiopulmonary haemodynamics at early follow-up in patients without, and to a lesser extent, with cardiovascular co-morbidities. This occurs in line with improvements of clinical parameters and risk status.
    Language English
    Publishing date 2024-01-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2814355-3
    ISSN 2055-5822 ; 2055-5822
    ISSN (online) 2055-5822
    ISSN 2055-5822
    DOI 10.1002/ehf2.14611
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  8. Book ; Online: Quantification of electronic and magnetoelastic mechanisms of first-order magnetic phase transitions from first principles

    Tapia, Eduardo Mendive / Patrick, Christopher E. / Hickel, Tilmann / Neugebauer, Jörg / Staunton, Julie B.

    application to caloric effects in La(Fe$_x$Si$_{1-x}$)$_{13}$

    2023  

    Abstract: La(Fe$_x$Si$_{1-x}$)$_{13}$ and derived quaternary compounds are well-known for their giant, tunable, magneto- and barocaloric responses around a first-order paramagnetic-ferromagnetic transition near room temperature with low hysteresis. Remarkably, ... ...

    Abstract La(Fe$_x$Si$_{1-x}$)$_{13}$ and derived quaternary compounds are well-known for their giant, tunable, magneto- and barocaloric responses around a first-order paramagnetic-ferromagnetic transition near room temperature with low hysteresis. Remarkably, such a transition shows a large spontaneous volume change together with itinerant electron metamagnetic features. While magnetovolume effects are well-established mechanisms driving first-order transitions, purely electronic sources have a long, subtle history and remain poorly understood. Here we apply a disordered local moment picture to quantify electronic and magnetoelastic effects at finite temperature in La(Fe$_x$Si$_{1-x}$)$_{13}$ from first-principles. We obtain results in very good agreement with experiment and demonstrate that the magnetoelastic coupling, rather than purely electronic mechanisms, drives the first-order character and causes at the same time a huge electronic entropy contribution to the caloric response.

    Comment: 20 pages, 6 figures
    Keywords Condensed Matter - Materials Science
    Subject code 530
    Publishing date 2023-02-13
    Publishing country us
    Document type Book ; Online
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  9. Book ; Online ; Thesis: Zur Interaktion des Epidermal Growth Factor mit Corticosteroiden bei der Regulation des L-Typ Ca2+ Stromes in isolierten linksventrikulären Kardiomyozyten der Ratte

    Frohberg, Andreas [Verfasser] / Volk, Tilmann [Akademischer Betreuer] / Volk, Tilmann [Gutachter] / Korbmacher, Christopher [Gutachter]

    2021  

    Author's details Andreas Frohberg ; Gutachter: Tilmann Volk, Christopher Korbmacher ; Betreuer: Tilmann Volk
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
    Publishing place Erlangen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  10. Article ; Online: A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing.

    Tong, Yaojun / Jørgensen, Tue S / Whitford, Christopher M / Weber, Tilmann / Lee, Sang Yup

    Nature communications

    2021  Volume 12, Issue 1, Page(s) 5206

    Abstract: CRISPR base editing is a powerful method to engineer bacterial genomes. However, it restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt a CRISPR-Prime Editing-based, DSB-free, versatile, and single-nucleotide ... ...

    Abstract CRISPR base editing is a powerful method to engineer bacterial genomes. However, it restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt a CRISPR-Prime Editing-based, DSB-free, versatile, and single-nucleotide resolution genetic manipulation toolkit for prokaryotes. It can introduce substitutions, deletions, insertions, and the combination thereof, both in plasmids and the chromosome of E. coli with high fidelity. Notably, under optimal conditions, the efficiency of 1-bp deletions reach up to 40%. Moreover, deletions of up to 97 bp and insertions up to 33 bp were successful with the toolkit in E. coli, however, efficiencies dropped sharply with increased fragment sizes. With a second guide RNA, our toolkit can achieve multiplexed editing albeit with low efficiency. Here we report not only a useful addition to the genome engineering arsenal for E. coli, but also a potential basis for the development of similar toolkits for other bacteria.
    MeSH term(s) CRISPR-Cas Systems ; Clustered Regularly Interspaced Short Palindromic Repeats ; DNA, Bacterial ; Escherichia coli/genetics ; Gene Editing/methods ; Genetic Engineering/methods ; Genome, Bacterial ; Plasmids ; RNA, Guide, CRISPR-Cas Systems/genetics
    Chemical Substances DNA, Bacterial ; RNA, Guide, CRISPR-Cas Systems
    Language English
    Publishing date 2021-09-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-021-25541-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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