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  1. Article: Cross-Generational Differences in Independence and Interdependence: Discrepancies Between Their Actual and Ideal Selves in the Japanese Cultural Context.

    Hashimoto, Hirofumi

    Frontiers in psychology

    2021  Volume 12, Page(s) 676526

    Abstract: The current study examined cross-generational differences in both independent and interdependent self-construal. Two studies using samples from across Japan that included a wide age range demonstrated that, with increasing age, Japanese respondents ... ...

    Abstract The current study examined cross-generational differences in both independent and interdependent self-construal. Two studies using samples from across Japan that included a wide age range demonstrated that, with increasing age, Japanese respondents scored higher on independence, which was measured by a self-expression scale, and lower on interdependence, as measured by a rejection avoidance scale. Furthermore, these cross-over effects were not observed with regard to participants' preferences (i.e., the ideal state of the self), but were only observed in their actual selves (i.e., the actual state of the self). These results suggest that the Japanese, especially younger generations, cannot help but behave in an interdependent way despite being eager to be independent.
    Language English
    Publishing date 2021-06-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2563826-9
    ISSN 1664-1078
    ISSN 1664-1078
    DOI 10.3389/fpsyg.2021.676526
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Mre11 exonuclease activity promotes irreversible mitotic progression under replication stress.

    Hashimoto, Yoshitami / Tanaka, Hirofumi

    Life science alliance

    2022  Volume 5, Issue 6

    Abstract: Mre11 is a versatile exo-/endonuclease involved in multiple aspects of DNA replication and repair, such as DSB end processing and checkpoint activation. We previously demonstrated that forced mitotic entry drives replisome disassembly at stalled ... ...

    Abstract Mre11 is a versatile exo-/endonuclease involved in multiple aspects of DNA replication and repair, such as DSB end processing and checkpoint activation. We previously demonstrated that forced mitotic entry drives replisome disassembly at stalled replication forks in
    MeSH term(s) Animals ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; DNA Replication ; Exonucleases/genetics ; Exonucleases/metabolism ; Protein-Tyrosine Kinases/metabolism ; Xenopus Proteins/genetics ; Xenopus Proteins/metabolism ; Xenopus laevis/genetics
    Chemical Substances Cell Cycle Proteins ; Xenopus Proteins ; WEE1 protein, Xenopus (EC 2.7.1.-) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; Exonucleases (EC 3.1.-)
    Language English
    Publishing date 2022-03-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202101249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Editorial: The role of culture in human thinking and reasoning.

    Yama, Hiroshi / Galbraith, Niall / Baratgin, Jean / Hashimoto, Hirofumi

    Frontiers in psychology

    2022  Volume 13, Page(s) 1018392

    Language English
    Publishing date 2022-09-13
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2563826-9
    ISSN 1664-1078
    ISSN 1664-1078
    DOI 10.3389/fpsyg.2022.1018392
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Regiospecificity of Immobilized Candida antarctica Lipase B (CAL-B) towards 2,3-Diacyl-1-O-alkyl Glyceryl Ether in Ethanol.

    Sato, Hirofumi / Hashimoto, Naoki / Watanabe, Yomi / Ohtaka, Atsushi

    Journal of oleo science

    2023  Volume 73, Issue 1, Page(s) 55–63

    Abstract: Highly pure 2,3-dioleoyl-1-O-alkyl glyceryl ether (DOGE), whose 1-position is a lipase-tolerant ether bond, was chemically synthesized and its detailed regioselectivity and acyl transfer were confirmed. During ethanolysis using immobilized Candida ... ...

    Abstract Highly pure 2,3-dioleoyl-1-O-alkyl glyceryl ether (DOGE), whose 1-position is a lipase-tolerant ether bond, was chemically synthesized and its detailed regioselectivity and acyl transfer were confirmed. During ethanolysis using immobilized Candida antarctica lipase B (CAL-B) with DOGE as the substrate, monooleoyl-1-O-alkyl glyceryl ethers (MOGEs) and a few 1-alkyl glyceryl ethers were formed upon consumption of the substrate. The structure of MOGE was confirmed using nuclear magnetic resonance spectroscopy and only the isomer of 2-MOGE was formed, indicating that CAL-B has complete α- regiospecificity. During ethanolysis, 3-MOGE was formed via acyl migration. These results indicate that the formation of 1-alkyl glyceryl ethers is not due to the imperfect regiospecificity of CAL-B, but rather due to ethanolysis of the formed 3-MOGE. The ethanolysis rate at the 3-α-position of DOGE was faster and the rate of acyl transfer was slightly slower for chain lengths greater than 14. These results show for the first time that both deacylation at the 3-position and acyl migration from the 2- to 3-position are affected by the structure of 1-position.
    MeSH term(s) Glyceryl Ethers ; Ethanol/chemistry ; Lipase/chemistry ; Fungal Proteins/chemistry ; Enzymes, Immobilized/chemistry
    Chemical Substances Glyceryl Ethers ; lipase B, Candida antarctica (EC 3.1.1.3) ; Ethanol (3K9958V90M) ; Lipase (EC 3.1.1.3) ; Fungal Proteins ; Enzymes, Immobilized
    Language English
    Publishing date 2023-12-14
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2218264-0
    ISSN 1347-3352 ; 1345-8957
    ISSN (online) 1347-3352
    ISSN 1345-8957
    DOI 10.5650/jos.ess23153
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Collegial Organizational Climate Alleviates Japanese Schoolteachers' Risk for Burnout.

    Hashimoto, Hirofumi / Maeda, Kaede

    Frontiers in psychology

    2021  Volume 12, Page(s) 737125

    Abstract: The purpose of the current study was to examine the influence of individuals' help-seeking preference (HSP) and their collective perception of the organizational climate in school on teachers' mental health. Previous studies demonstrated that HSP was ... ...

    Abstract The purpose of the current study was to examine the influence of individuals' help-seeking preference (HSP) and their collective perception of the organizational climate in school on teachers' mental health. Previous studies demonstrated that HSP was negatively associated with risk of burnout, suggesting that teachers who hesitate to seek help from their colleagues are more likely to have mental health problems. Thus, the current study hypothesized that a collegial organizational climate would be negatively associated with burnout. To test this hypothesis, we developed a scale to measure schoolteachers' collective perception of their organizational climate (Study 1), and the newly developed scale was used to assess its relationship with HSP and teachers' burnout risk (Study 2). The results demonstrated that younger teachers, a low level of help-seeking, and a less collaborative climate increased the risk of burnout. The results also showed a significant interaction effect, indicating that HSP was less closely associated with teachers' burnout risk if their organization was perceived as having a collegial climate. These findings clearly show how the social environment of a school's organizational climate can affect schoolteachers' mental health in Japan.
    Language English
    Publishing date 2021-12-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2563826-9
    ISSN 1664-1078
    ISSN 1664-1078
    DOI 10.3389/fpsyg.2021.737125
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Preconditioning-Induced Facilitation of Lactate Release from Astrocytes Is Essential for Brain Ischemic Tolerance.

    Hirayama, Yuri / Le, Ha Pham Ngoc / Hashimoto, Hirofumi / Ishii, Itsuko / Koizumi, Schuichi / Anzai, Naohiko

    eNeuro

    2024  Volume 11, Issue 4

    Abstract: A sublethal ischemic episode [termed preconditioning (PC)] protects neurons in the brain against a subsequent severe ischemic injury. This phenomenon is known as brain ischemic tolerance and has received much attention from researchers because of its ... ...

    Abstract A sublethal ischemic episode [termed preconditioning (PC)] protects neurons in the brain against a subsequent severe ischemic injury. This phenomenon is known as brain ischemic tolerance and has received much attention from researchers because of its robust neuroprotective effects. We have previously reported that PC activates astrocytes and subsequently upregulates P2X7 receptors, thereby leading to ischemic tolerance. However, the downstream signals of P2X7 receptors that are responsible for PC-induced ischemic tolerance remain unknown. Here, we show that PC-induced P2X7 receptor-mediated lactate release from astrocytes has an indispensable role in this event. Using a transient focal cerebral ischemia model caused by middle cerebral artery occlusion, extracellular lactate levels during severe ischemia were significantly increased in mice who experienced PC; this increase was dependent on P2X7 receptors. In addition, the intracerebroventricular injection of lactate protected against cerebral ischemic injury. In in vitro experiments, although stimulation of astrocytes with the P2X7 receptor agonist BzATP had no effect on the protein levels of monocarboxylate transporter (MCT) 1 and MCT4 (which are responsible for lactate release from astrocytes), BzATP induced the plasma membrane translocation of these MCTs via their chaperone CD147. Importantly, CD147 was increased in activated astrocytes after PC, and CD147-blocking antibody abolished the PC-induced facilitation of astrocytic lactate release and ischemic tolerance. Taken together, our findings suggest that astrocytes induce ischemic tolerance via P2X7 receptor-mediated lactate release.
    MeSH term(s) Animals ; Astrocytes/metabolism ; Astrocytes/drug effects ; Ischemic Preconditioning/methods ; Lactic Acid/metabolism ; Lactic Acid/pharmacology ; Receptors, Purinergic P2X7/metabolism ; Male ; Monocarboxylic Acid Transporters/metabolism ; Mice, Inbred C57BL ; Basigin/metabolism ; Brain Ischemia/metabolism ; Symporters/metabolism ; Infarction, Middle Cerebral Artery/metabolism ; Disease Models, Animal ; Muscle Proteins/metabolism ; Adenosine Triphosphate/metabolism ; Adenosine Triphosphate/pharmacology ; Mice ; Cells, Cultured ; Brain/metabolism ; Mice, Knockout
    Chemical Substances Lactic Acid (33X04XA5AT) ; Receptors, Purinergic P2X7 ; Monocarboxylic Acid Transporters ; Basigin (136894-56-9) ; Slc16a4 protein, mouse ; monocarboxylate transport protein 1 ; Bsg protein, mouse ; Symporters ; Muscle Proteins ; Adenosine Triphosphate (8L70Q75FXE) ; P2rx7 protein, mouse
    Language English
    Publishing date 2024-04-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2800598-3
    ISSN 2373-2822 ; 2373-2822
    ISSN (online) 2373-2822
    ISSN 2373-2822
    DOI 10.1523/ENEURO.0494-23.2024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Simultaneous recording of behavioral and neural responses of free-moving nematodes

    Sato, Hirofumi / Kunitomo, Hirofumi / Fei, Xianfeng / Hashimoto, Koichi / Iino, Yuichi

    STAR protocols

    2021  Volume 2, Issue 4, Page(s) 101011

    Abstract: To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely ... ...

    Abstract To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving
    MeSH term(s) Animals ; Behavior, Animal/drug effects ; Behavior, Animal/physiology ; Caenorhabditis elegans/drug effects ; Caenorhabditis elegans/physiology ; Calcium/metabolism ; Chemotaxis/drug effects ; Chemotaxis/physiology ; Microfluidic Analytical Techniques/methods ; Molecular Imaging ; Sodium Chloride/pharmacology
    Chemical Substances Sodium Chloride (451W47IQ8X) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-12-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2021.101011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation.

    Hashimoto, Yoshitami / Sadano, Kota / Miyata, Nene / Ito, Haruka / Tanaka, Hirofumi

    The EMBO journal

    2023  Volume 42, Issue 17, Page(s) e114131

    Abstract: CMG (Cdc45-MCM-GINS) helicase assembly at the replication origin is the culmination of eukaryotic DNA replication initiation. This process can be reconstructed in vitro using defined factors in Saccharomyces cerevisiae; however, in vertebrates, origin- ... ...

    Abstract CMG (Cdc45-MCM-GINS) helicase assembly at the replication origin is the culmination of eukaryotic DNA replication initiation. This process can be reconstructed in vitro using defined factors in Saccharomyces cerevisiae; however, in vertebrates, origin-dependent CMG formation has not yet been achieved partly due to the lack of a complete set of known initiator proteins. Since a microcephaly gene product, DONSON, was reported to remodel the CMG helicase under replication stress, we analyzed its role in DNA replication using a Xenopus cell-free system. We found that DONSON was essential for the replisome assembly. In vertebrates, DONSON physically interacted with GINS and Polε via its conserved N-terminal PGY and NPF motifs, and the DONSON-GINS interaction contributed to the replisome assembly. DONSON's chromatin association during replication initiation required the pre-replicative complex, TopBP1, and kinase activities of S-CDK and DDK. Both S-CDK and DDK required DONSON to trigger replication initiation. Moreover, human DONSON could substitute for the Xenopus protein in a cell-free system. These findings indicate that vertebrate DONSON is a novel initiator protein essential for CMG helicase assembly.
    MeSH term(s) Animals ; Humans ; Minichromosome Maintenance Proteins/genetics ; Minichromosome Maintenance Proteins/metabolism ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Saccharomyces cerevisiae Proteins/metabolism ; DNA Replication ; Saccharomyces cerevisiae/metabolism ; Vertebrates
    Chemical Substances Minichromosome Maintenance Proteins (EC 3.6.4.12) ; Cell Cycle Proteins ; DNA-Binding Proteins ; Saccharomyces cerevisiae Proteins
    Language English
    Publishing date 2023-07-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.15252/embj.2023114131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Simultaneous recording of behavioral and neural responses of free-moving nematodes C. elegans

    Hirofumi Sato / Hirofumi Kunitomo / Xianfeng Fei / Koichi Hashimoto / Yuichi Iino

    STAR Protocols, Vol 2, Iss 4, Pp 101011- (2021)

    2021  

    Abstract: Summary: To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely ...

    Abstract Summary: To reveal the neural mechanisms that control animal behavior, it is necessary to link the neural responses to behavioral changes and interpret them. We have developed a protocol to simultaneously record the behavior and neural activity of freely moving C. elegans by combining a microfluidic device and a tracking stage. Here we detail the protocol for the experiment, with an example of behavioral and neural responses of nematodes to salt concentration changes.For complete details on the use and execution of this protocol, please refer to Sato et al. (2021).
    Keywords Microscopy ; Model Organisms ; Neuroscience ; Behavior ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Glutamate signaling from a single sensory neuron mediates experience-dependent bidirectional behavior in Caenorhabditis elegans.

    Sato, Hirofumi / Kunitomo, Hirofumi / Fei, Xianfeng / Hashimoto, Koichi / Iino, Yuichi

    Cell reports

    2021  Volume 35, Issue 8, Page(s) 109177

    Abstract: Orientation and navigation behaviors of animals are modulated by past experiences. However, little is known about the mechanisms by which sensory inputs are translated into multi-directional orientation behaviors in an experience-dependent manner. Here, ... ...

    Abstract Orientation and navigation behaviors of animals are modulated by past experiences. However, little is known about the mechanisms by which sensory inputs are translated into multi-directional orientation behaviors in an experience-dependent manner. Here, we report a neural mechanism for bidirectional salt-concentration chemotaxis of Caenorhabditis elegans. The salt-sensing neuron ASE right (ASER) is always activated by a decrease of salt concentration, while the directionality of reorientation behaviors is inverted depending on previous salt experiences. AIB, the interneuron postsynaptic to ASER, and neurons farther downstream of AIB show experience-dependent bidirectional responses, which are correlated with reorientation behaviors. These bidirectional behavioral and neural responses are mediated by glutamate released from ASER. Glutamate acts through the excitatory glutamate receptor GLR-1 and inhibitory glutamate receptor AVR-14, both acting in AIB. These findings suggest that experience-dependent reorientation behaviors are generated by altering the magnitude of excitatory and inhibitory postsynaptic signals from a sensory neuron to interneurons.
    MeSH term(s) Animals ; Caenorhabditis elegans ; Glutamates/metabolism ; Sensory Receptor Cells/metabolism ; Signal Transduction
    Chemical Substances Glutamates
    Language English
    Publishing date 2021-06-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2021.109177
    Database MEDical Literature Analysis and Retrieval System OnLINE

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