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  1. Article ; Online: Heterozygous UDP-GlcNAc 2-epimerase and N-acetylmannosamine kinase domain mutations in the GNE gene result in a less severe GNE myopathy phenotype compared to homozygous N-acetylmannosamine kinase domain mutations.

    Mori-Yoshimura, Madoka / Monma, Kazunari / Suzuki, Naoki / Aoki, Masashi / Kumamoto, Toshihide / Tanaka, Keiko / Tomimitsu, Hiroyuki / Nakano, Satoshi / Sonoo, Masahiro / Shimizu, Jun / Sugie, Kazuma / Nakamura, Harumasa / Oya, Yasushi / Hayashi, Yukiko K / Malicdan, May Christine V / Noguchi, Satoru / Murata, Miho / Nishino, Ichizo

    Journal of the neurological sciences

    2012  Volume 318, Issue 1-2, Page(s) 100–105

    Abstract: Background: Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE) myopathy, also ... disease onset. Participants with a homozygous mutation (p.V572L) in the N-acetylmannosamine kinase domain ... with mutations in the uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) 2-epimerase and N-acetylmannosamine ...

    Abstract Background: Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE) myopathy, also called distal myopathy with rimmed vacuoles (DMRV) or hereditary inclusion body myopathy (HIBM), is a rare, progressive autosomal recessive disorder caused by mutations in the GNE gene. Here, we examined the relationship between genotype and clinical phenotype in participants with GNE myopathy.
    Methods: Participants with GNE myopathy were asked to complete a questionnaire regarding medical history and current symptoms.
    Results: A total of 71 participants with genetically confirmed GNE myopathy (27 males and 44 females; mean age, 43.1±13.0 (mean±SD) years) completed the questionnaire. Initial symptoms (e.g., foot drop and lower limb weakness) appeared at a mean age of 24.8±8.3 years. Among the 71 participants, 11 (15.5%) had the ability to walk, with a median time to loss of ambulation of 17.0±2.1 years after disease onset. Participants with a homozygous mutation (p.V572L) in the N-acetylmannosamine kinase domain (KD/KD participants) had an earlier disease onset compared to compound heterozygous participants with mutations in the uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) 2-epimerase and N-acetylmannosamine kinase domains (ED/KD participants; 26.3±7.3 vs. 21.2±11.1 years, respectively). KD/KD participants were more frequently non-ambulatory compared to ED/KD participants at the time of survey (80% vs. 50%). Data were verified using medical records available from 17 outpatient participants.
    Conclusions: Homozygous KD/KD participants exhibited a more severe phenotype compared to heterozygous ED/KD participants.
    MeSH term(s) Adolescent ; Adult ; Aged ; Carbohydrate Epimerases/genetics ; Carbohydrate Epimerases/metabolism ; Female ; Heterozygote ; Homozygote ; Humans ; Male ; Middle Aged ; Multienzyme Complexes/genetics ; Multienzyme Complexes/metabolism ; Phenotype ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Phosphotransferases (Alcohol Group Acceptor)/metabolism ; Protein Structure, Tertiary/genetics ; Severity of Illness Index ; Young Adult
    Chemical Substances Multienzyme Complexes ; UDP-N-acetylglucosamine 2-epimerase - N-acetylmannosamine kinase ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-) ; N-acylmannosamine kinase (EC 2.7.1.60) ; Carbohydrate Epimerases (EC 5.1.3.-) ; UDP acetylglucosamine-2-epimerase (EC 5.1.3.14)
    Language English
    Publishing date 2012-07-15
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80160-4
    ISSN 1878-5883 ; 0022-510X ; 0374-8642
    ISSN (online) 1878-5883
    ISSN 0022-510X ; 0374-8642
    DOI 10.1016/j.jns.2012.03.016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Chylothorax Associated with the Syndrome of Idiopathic Osteolysis Including Gorham-Stout Disease: Spontaneous Remission Without Treatment.

    Monma, Sota / Yano, Michihiro / Matsuda, Masazumi / Tseuchie, Hiroyuki / Mori, Naoko

    Turkish archives of pediatrics

    2024  Volume 59, Issue 2, Page(s) 230–231

    Language English
    Publishing date 2024-03-07
    Publishing country Turkey
    Document type Journal Article
    ISSN 2757-6256
    ISSN 2757-6256
    DOI 10.5152/TurkArchPediatr.2024.23208
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  3. Article ; Online: Enhanced responses to inflammatory cytokine interleukin-6 in micropatterned networks of cultured cortical neurons.

    Sakaibara, Mamoru / Yamamoto, Hideaki / Murota, Hakuba / Monma, Nobuaki / Sato, Shigeo / Hirano-Iwata, Ayumi

    Biochemical and biophysical research communications

    2023  Volume 695, Page(s) 149379

    Abstract: Cortical neurons in dissociated cultures are an indispensable model system for pharmacological research that provides insights into chemical responses in well-defined environments. However, cortical neurons plated on homogeneous substrates develop an ... ...

    Abstract Cortical neurons in dissociated cultures are an indispensable model system for pharmacological research that provides insights into chemical responses in well-defined environments. However, cortical neurons plated on homogeneous substrates develop an unstructured network that exhibits excessively synchronized activity, which occasionally masks the consequences induced by external substances. Here, we show that hyperactivity and excessive synchrony in cultured cortical networks can be effectively suppressed by growing neurons in microfluidic devices. These devices feature a hierarchically modular design that resembles the in vivo network. We focused on interleukin-6, a pro-inflammatory cytokine, and assessed its acute and chronic effects. Fluorescence calcium imaging of spontaneous neural activity for up to 20 days of culture showed detectable modulation of collective activity events and neural correlation in micropatterned neurons, which was not apparent in neurons cultured on homogeneous substrates. Our results indicate that engineered neuronal networks provide a unique platform for detecting and understanding the fundamental effects of biochemical compounds on neuronal networks.
    MeSH term(s) Interleukin-6/pharmacology ; Cytokines/pharmacology ; Action Potentials/physiology ; Cells, Cultured ; Nerve Net ; Neurons
    Chemical Substances Interleukin-6 ; Cytokines
    Language English
    Publishing date 2023-12-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2023.149379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Acute effect of corticosterone on N-methyl-D-aspartate receptor-mediated Ca2+ elevation in mouse hippocampal slices.

    Sato, Satoru / Osanai, Hiromi / Monma, Toshihiro / Harada, Tokiko / Hirano, Ayumi / Saito, Minoru / Kawato, Suguru

    Biochemical and biophysical research communications

    2004  Volume 321, Issue 2, Page(s) 510–513

    Abstract: We examined the rapid effects of corticosterone (CORT) on N-methyl-D-aspartate (NMDA) receptor ...

    Abstract We examined the rapid effects of corticosterone (CORT) on N-methyl-D-aspartate (NMDA) receptor-mediated Ca2+ signals in adult mouse hippocampal slices by using Ca2+ imaging technique. Application of NMDA caused a transient elevation of intracellular Ca2+ concentration followed by a decay to a plateau within 150s. The 30min preincubation of CORT induced a significant decrease of the peak amplitude of NMDA-induced Ca2+ elevation in the CA1 region. The rapid effect of CORT was induced at a stress-induced level (0.4-10microM). Because the membrane non-permeable bovine serum albumin-conjugated CORT also induced a similar rapid effect, the rapid effect of CORT might be induced via putative surface CORT receptors. In contrast, CORT induced no significant effects on NMDA-induced Ca2+ elevation in the dentate gyrus. In the CA3 region, CORT effects were not evaluated, because the marked elevation of NMDA-induced Ca2+ signals was not observed there.
    MeSH term(s) Animals ; Calcium/metabolism ; Calcium Signaling/drug effects ; Corticosterone/pharmacology ; Hippocampus/drug effects ; Hippocampus/metabolism ; In Vitro Techniques ; Male ; Mice ; N-Methylaspartate/pharmacology ; Receptors, N-Methyl-D-Aspartate/metabolism
    Chemical Substances Receptors, N-Methyl-D-Aspartate ; N-Methylaspartate (6384-92-5) ; Calcium (SY7Q814VUP) ; Corticosterone (W980KJ009P)
    Language English
    Publishing date 2004-08-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 205723-2
    ISSN 0006-291X ; 0006-291X
    ISSN (online) 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2004.06.168
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  5. Article: Antiemetic effects of N-3389, a newly synthesized 5-HT3 and 5-HT4 receptor antagonist, in ferrets.

    Minami, M / Endo, T / Tamakai, H / Ogawa, T / Hamaue, N / Hirafuji, M / Monma, Y / Yoshioka, M / Hagihara, K

    European journal of pharmacology

    1997  Volume 321, Issue 3, Page(s) 333–342

    Abstract: The antiemetic activity of N-3389 (endo-3,9-dimethyl-3,9-diazabicyclo[3,3,1]non-7-yl-1 H-indazole-3 ... mg/kg) and oral (0.1-1.0 mg/kg) administration of N-3389 produced dose-dependent antiemetic effects ... by pretreatment with N-3389 (1.0 mg/kg, i.v.). Furthermore, the cisplatin (10 mg/kg, i.p.)-induced increase ...

    Abstract The antiemetic activity of N-3389 (endo-3,9-dimethyl-3,9-diazabicyclo[3,3,1]non-7-yl-1 H-indazole-3-carboxamide dihydrochloride), a new 5-HT3 and 5-HT4 receptor antagonist, against cisplatin-, cyclophosphamide- and copper sulfate-induced emesis was investigated using ferrets. We also examined the effects of these agents on abdominal afferent vagus nerve activity in anesthetized ferrets. Both intraperitoneal (0.1-1.0 mg/kg) and oral (0.1-1.0 mg/kg) administration of N-3389 produced dose-dependent antiemetic effects. The time-course of cisplatin (10 mg/kg, i.p.)-induced emesis in another group of ferrets paralleled the increase in abdominal afferent vagus nerve activity induced by cisplatin (10 mg/kg, i.p.) and was inhibited by pretreatment with N-3389 (1.0 mg/kg, i.v.). Furthermore, the cisplatin (10 mg/kg, i.p.)-induced increase in abdominal afferent vagus nerve activity was markedly reduced by an additional injection of N-3389 (0.1-1.0 mg/kg, i.v.) in a dose-dependent manner. The antiemetic effects exhibited by N-3389 are probably due to the inhibition of 5-HT3 and 5-HT4 receptors on the abdominal afferent vagus nerves.
    MeSH term(s) 5-Methoxytryptamine/pharmacology ; Animals ; Antiemetics/pharmacology ; Antineoplastic Agents/toxicity ; Bridged Bicyclo Compounds, Heterocyclic/pharmacology ; Cisplatin/toxicity ; Copper Sulfate/toxicity ; Cyclophosphamide/toxicity ; Emetics/toxicity ; Ferrets ; Indazoles/pharmacology ; Male ; Receptors, Serotonin/drug effects ; Receptors, Serotonin, 5-HT3 ; Receptors, Serotonin, 5-HT4 ; Serotonin/analogs & derivatives ; Serotonin/pharmacology ; Serotonin Antagonists/pharmacology ; Vagus Nerve/drug effects ; Vagus Nerve/physiology ; Vomiting/chemically induced ; Vomiting/drug therapy
    Chemical Substances Antiemetics ; Antineoplastic Agents ; Bridged Bicyclo Compounds, Heterocyclic ; Emetics ; Indazoles ; Receptors, Serotonin ; Receptors, Serotonin, 5-HT3 ; Serotonin Antagonists ; Receptors, Serotonin, 5-HT4 (158165-40-3) ; Serotonin (333DO1RDJY) ; 5-Methoxytryptamine (3VMW6141KC) ; 2-methyl-5-HT (78263-90-8) ; Cyclophosphamide (8N3DW7272P) ; Copper Sulfate (LRX7AJ16DT) ; N 3389 (NBN3HB12DW) ; Cisplatin (Q20Q21Q62J)
    Language English
    Publishing date 1997-03-05
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80121-5
    ISSN 1879-0712 ; 0014-2999
    ISSN (online) 1879-0712
    ISSN 0014-2999
    DOI 10.1016/s0014-2999(96)00974-0
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  6. Article ; Online: Fewer systematic prostate core biopsies in clinical stage T1c prostate cancer leads to biochemical recurrence after brachytherapy as monotherapy.

    Ozawa, Yu / Nohara, Sunao / Nakamura, Ken / Hattori, Seiya / Yagi, Yasuto / Nishiyama, Toru / Yorozu, Atsunori / Monma, Tetsuo / Saito, Shiro

    The Prostate

    2024  Volume 84, Issue 5, Page(s) 502–510

    Abstract: ... into the biopsy cores ≤8 (N = 123) and ≥9 (N = 393) groups. The BCR-free survival rate was compared between ...

    Abstract Background: After brachytherapy, fewer prostate biopsy cores at diagnosis can underestimate the pathological characteristics of prostate cancer (PCa) with lower concordance, resulting in improper treatment, particularly in patients with low-risk nonpalpable cT1c PCa. The aim of this study was to assess the relationship between the number of biopsy cores at diagnosis and long-term clinical outcomes after brachytherapy for cT1c PCa.
    Methods: We reviewed 516 patients with localized cT1c PCa with Gleason scores of 3 + 3 = 6 or 3 + 4 = 7 who underwent brachytherapy as monotherapy without hormonal therapy between January 2005 and September 2014 at our institution. Clinical staging was based on the American Joint Committee on Cancer manual for staging. Thus, the cT1c category is based solely on digital rectal examination. The primary outcome was biochemical recurrence (BCR). Based on the optimized cutoff value for biopsy core number obtained from receiver operating characteristic analysis, patients were divided into the biopsy cores ≤8 (N = 123) and ≥9 (N = 393) groups. The BCR-free survival rate was compared between the groups. Prognostic factors for BCR were evaluated, including age, initial prostate-specific antigen (PSA) level, Gleason score, positive core rate, PSA density, prostate magnetic resonance imaging findings, and biopsy core number.
    Results: The median patient age was 66.0 years (interquartile range [IQR]: 61.0-71.0 years), and the median follow-up time was 11.1 years (IQR: 9.5-13.3 years). The median number of core biopsies was 12 (IQR: 9-12). The area under the curve was 0.637 (95% confidence interval [CI]: 0.53-0.75), and the optimal biopsy core cutoff value for BCR prediction was 8.5 (sensitivity = 43.5%, specificity = 77.1%). Although fewer patients had Gleason scores of 3 + 4 = 7 (19/123 [15%] vs. 125/393 [32%], p < 0.02) in the biopsy cores ≤8 group, the 10-year BCR-free survival rate was significantly lower in the biopsy cores ≤8 group than in the biopsy cores ≥9 group (93.8% vs. 96.3%, p < 0.05). Multivariate analysis revealed that a lower biopsy core number (hazard ratio: 0.828, 95% CI: 0.71-0.97, p < 0.03) and a Gleason score of 3 + 4 = 7 (hazard ratio: 3.26, 95% CI: 1.37-7.73, p < 0.01) significantly predicted BCR.
    Conclusions: A low number of prostate core biopsies results in worse BCR-free survival after brachytherapy as monotherapy in patients with cT1c PCa.
    MeSH term(s) Male ; Humans ; Middle Aged ; Aged ; Brachytherapy/methods ; Prostate-Specific Antigen ; Prostate/pathology ; Prostatic Neoplasms ; Biopsy ; Neoplasm Staging
    Chemical Substances Prostate-Specific Antigen (EC 3.4.21.77)
    Language English
    Publishing date 2024-01-03
    Publishing country United States
    Document type Review ; Journal Article
    ZDB-ID 604707-5
    ISSN 1097-0045 ; 0270-4137
    ISSN (online) 1097-0045
    ISSN 0270-4137
    DOI 10.1002/pros.24668
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  7. Article: [A Case of Rectal Cancer with Hemorrhagic Shock with Bleeding Controlled via Stent Placement That Underwent Radical Resection after Preoperative CRT].

    Ami, Katsunori / Suyama, Yuto / Nakanishi, Akihito / Hakozaki, Tomoki / Harada, Toshiko / Monma, Satoko / Tamura, Noriyasu / Ooide, Kei / Machinami, Rikuo / Umetani, Naoaki

    Gan to kagaku ryoho. Cancer & chemotherapy

    2023  Volume 49, Issue 13, Page(s) 1488–1490

    Abstract: Colorectal stents are used mainly for the palliative treatment of colorectal obstruction or preoperative re-obstruction. However, the hemostatic effect of covered stents reportedly induced bleeding of esophageal cancer and varicosities. Here, we report a ...

    Abstract Colorectal stents are used mainly for the palliative treatment of colorectal obstruction or preoperative re-obstruction. However, the hemostatic effect of covered stents reportedly induced bleeding of esophageal cancer and varicosities. Here, we report a case of mildly obstructed rectal cancer with severe anemia and hemorrhagic shock that resulted in pulsatile tumor bleeding. Curative surgical resection was performed successfully after the administration of chemoradiotherapy. The patient was a 67-year-old man. A nearby doctor diagnosed him with anemia(Hb 4.6 g/dL)and referred him to our hospital, where he was diagnosed with rectal cancer at the Ra position. He was immediately hospitalized owing to voluminous melena, loss of consciousness, and hematoma formation on the posterior aspect of the head. Urgent CF was performed due to persistent melena and decreased blood pressure. The pulsatile bleeding from rectal cancer was identified. To address the diffuse bleeding, a covered stent was placed to induce hemostasis and dilation. This also served as a bridge to surgery( BTS). Hemostasis was successfully achieved. After chemoradiotherapy( CRT), a laparoscopic low anterior resection was performed. Radical surgery was performed, and S-1 was taken 6 months postoperatively. At 2 years postoperatively, metastatic recurrence was not observed.
    MeSH term(s) Humans ; Male ; Aged ; Shock, Hemorrhagic ; Melena ; Rectal Neoplasms/complications ; Rectal Neoplasms/surgery ; Chemoradiotherapy ; Hemorrhage ; Anemia ; Stents
    Language Japanese
    Publishing date 2023-01-24
    Publishing country Japan
    Document type Case Reports ; English Abstract ; Journal Article
    ZDB-ID 604842-0
    ISSN 0385-0684
    ISSN 0385-0684
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  8. Article ; Online: Development of adolescent childhood cancer survivors' psychosocial issues scale.

    Hidaka, Kyoko / Monma, Takafumi / Fukushima, Hiroko / Ozawa, Noriko / Iwata, Naoko / Terada, Kazuki / Fukushima, Takashi / Takeda, Fumi

    Pediatrics international : official journal of the Japan Pediatric Society

    2023  Volume 65, Issue 1, Page(s) e15664

    Abstract: Background: Follow-up care for adolescent childhood cancer survivors (ACCS) after they return to school requires an understanding of their psychosocial issues. Therefore, this study developed the adolescent childhood cancer survivors' psychosocial ... ...

    Abstract Background: Follow-up care for adolescent childhood cancer survivors (ACCS) after they return to school requires an understanding of their psychosocial issues. Therefore, this study developed the adolescent childhood cancer survivors' psychosocial issues scale (ACCSPIS) and evaluated its reliability and validity.
    Methods: In the development phase, pediatric oncology clinical professionals created the 24 item questionnaire of ACCS's psychosocial issues. In the feasibility phase, a survey was administered to 165 ACCS aged 12-18 years after discharge from hospital in Japan, and 57 completed questionnaires were analyzed. The survey items were psychosocial issues, attributes, K6 scale, and impact of event scale-revised (IES-R) scale. Factor analysis was conducted for psychosocial issues. Regarding reliability, Cronbach's α coefficients and item-total correlation coefficients were calculated. Regarding validity, Spearman's rank correlation coefficients between ACCSPIS and K6 and IES-R were calculated, and confirmatory factor analysis was conducted.
    Results: Four factors comprising 15 items were extracted: "appearance changes due to treatment effects," "anxiety about marriage and the future," "change in appearance due to treatment", and "psychological distress due to interpersonal relationships and information about the disease." The model fit was good, with a total ACCSPIS α coefficient of 0.901 and α coefficients for the subscales ranging from 0.651 to 0.914. The K6 and IES-R were significantly associated with the total ACCSPIS, and item-total correlations were satisfactory.
    Conclusions: The reliability and validity of ACCSPIS were generally confirmed. This scale could be useful to measure psychosocial issues in ACCS aged 12-18 years after their return to school.
    MeSH term(s) Humans ; Adolescent ; Child ; Cancer Survivors ; Reproducibility of Results ; Neoplasms/therapy ; Neoplasms/psychology ; Surveys and Questionnaires ; Anxiety ; Psychometrics
    Language English
    Publishing date 2023-11-21
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 1470376-2
    ISSN 1442-200X ; 1328-8067
    ISSN (online) 1442-200X
    ISSN 1328-8067
    DOI 10.1111/ped.15664
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  9. Article: Prevalence of Poor Sleep Quality and its Association with Lifestyle Habits, Competition-Based Activities, and Psychological Distress in Japanese Student-Athletes During the COVID-19 Pandemic.

    Monma, Takafumi / Yamashita, Kazuki / Yoshida, Goichiro / Fujita, Eiji / Miyazawa, Taiki / Ebine, Naoyuki / Matsukura, Keita / Takeda, Satoko / Matamura, Misato / Yamane, Maki / Ando, Kayoko / Omi, Naomi / Sagayama, Hiroyuki / Takeda, Fumi

    Sleep science (Sao Paulo, Brazil)

    2024  Volume 17, Issue 1, Page(s) e26–e36

    Abstract: ... ...

    Abstract Objectives
    Language English
    Publishing date 2024-02-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2697016-8
    ISSN 1984-0063 ; 1984-0659
    ISSN (online) 1984-0063
    ISSN 1984-0659
    DOI 10.1055/s-0043-1772811
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  10. Article: Development of a simple screening method for analyzing cereulide toxin in fried rice using liquid chromatography-tandem mass spectrometry.

    Koike, Hiroshi / Kanda, Maki / Monma, Chie / Yoshikawa, Souichi / Hayashi, Hiroshi / Matsushima, Yoko / Ohba, Yumi / Hayashi, Momoka / Furuta, Natsumi / Okada, Wakaba / Nagano, Chieko / Yokoyama, Keiko / Yokoyama, Tomoko / Sasamoto, Takeo

    Forensic toxicology

    2024  

    Abstract: Purpose: The presence of cereulide, an emetic toxin produced by Bacillus cereus, in fried rice samples is critical evidence of food poisoning even in situations where B. cereus could not be detected. This study aims to develop a screening method for ... ...

    Abstract Purpose: The presence of cereulide, an emetic toxin produced by Bacillus cereus, in fried rice samples is critical evidence of food poisoning even in situations where B. cereus could not be detected. This study aims to develop a screening method for analyzing cereulide in fried rice using the QuEChERS procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
    Methods: Cereulide was identified and quantified in fried rice samples using the QuEChERS extraction method and LC-MS/MS. The accuracies of the methods were determined by analyzing fortified blank samples at two concentrations (10 and 50 µg/kg) conducted on three samples daily for five days.
    Results: The QuEChERS procedure removed matrix compounds from fried rice. Characteristic MS/MS spectra enabled the identification of cereulide. As the matrix effects in seven fried rice samples were within ± 6%, an external solvent calibration curve could be used for quantification. This method exhibited good accuracy ranging from 88 to 89%. The relative standard deviations for both repeatability and intra-laboratory reproducibility were < 4%. These standard deviations satisfied the criteria of the Japanese validation guidelines for residues (MHLW 2010, Director Notice, Syoku-An No. 1224-1). The limit of quantification was 2 μg/kg. The applicability of this method was confirmed using the analysis of cereulide in fried rice samples incubated with emetic Bacillus cereus.
    Conclusions: The QuEChERS extraction procedure described herein showed substantial promise as a reliable screening tool for cereulide in fried rice sample.
    Language English
    Publishing date 2024-03-22
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2274095-8
    ISSN 1860-8965
    ISSN 1860-8965
    DOI 10.1007/s11419-024-00683-3
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