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  1. Article ; Online: Tomato puree quality from field to can: Effects of water and nitrogen-saving strategies

    Sinkora, Miarka / Fanciullino, Anne-Laure / Page, David / Giovinazzo, Robert / Lanoë, Lucas / Vilas Boas, Alexandre / Bertin, Nadia

    European Journal of Agronomy. 2023 Sept., v. 149 p.126891-

    2023  

    Abstract: Processing tomato is a main source of phytomicronutrients in the human diet due to its micronutrient content, and the diversity of derived products consumed worldwide. A large proportion of processing tomatoes are grown under Mediterranean climate where ... ...

    Abstract Processing tomato is a main source of phytomicronutrients in the human diet due to its micronutrient content, and the diversity of derived products consumed worldwide. A large proportion of processing tomatoes are grown under Mediterranean climate where increasing water scarcity and heat are expected to reduce yields and to have negative impacts on fruit quality. Despite this, the effects of changes in fresh fruit quality on processing and end-product quality have been under-investigated. It therefore seems imperative to work along the food chain to improve food sustainability while maintaining quality and access to important phytonutrients. The present study adopts a holistic approach from field to processing plant, assessing the effects of reduced water and nitrogen supplies on yields, fresh fruit quality and suitability for processing, and puree quality. The 5-year trial included eight varieties, several levels of water and nitrogen supply, and two processes. It was found that water deficit induced a decrease in fresh commercial yield, partially compensated by an increase in dry matter content. The yield loss was the result of a lower number of fruits per plant, while the average fruit weight and biochemical composition showed little change. Water use efficiency decreased above 120% of water demand restoration and improved under low nitrogen supply. Puree viscosity and dry matter content increased with water deficit with beneficial effects for the industry, while the reduction of nitrogen supply did not affect puree quality. This work highlighted the possibility of monitoring quality traits in tomato puree directly from field. Nevertheless, the trade-offs between WUE, quality and yield need to be optimized. Selection of varieties rich in lycopene could be a promising avenue as they appear more tolerant to water deficit. This work also stressed the need to reassess the crop coefficient for new varieties, to improve evaluation of water needs.
    Keywords Mediterranean climate ; agronomy ; crop coefficient ; dry matter content ; food chain ; fruit quality ; fruit weight ; heat ; human nutrition ; industry ; lycopene ; nitrogen ; phytonutrients ; raw fruit ; sustainable agriculture ; tomato puree ; tomatoes ; viscosity ; water shortages ; water use efficiency ; Water deficit ; Processing tomato ; Nitrogen fertilization ; Puree viscosity ; Inter-year variability
    Language English
    Dates of publication 2023-09
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 1157136-6
    ISSN 1873-7331 ; 1161-0301
    ISSN (online) 1873-7331
    ISSN 1161-0301
    DOI 10.1016/j.eja.2023.126891
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Faecal carriage of extended-spectrum β-lactamase-producing Escherichia coli in a remote region of Niger.

    Jacquier, Hervé / Assao, Bachir / Chau, Françoise / Guindo, Ousmane / Condamine, Bénédicte / Magnan, Mélanie / Bridier-Nahmias, Antoine / Sayingoza-Makombe, Nathan / Moumouni, Aissatou / Page, Anne-Laure / Langendorf, Céline / Coldiron, Matthew E / Denamur, Erick / de Lastours, Victoire

    The Journal of infection

    2023  Volume 87, Issue 3, Page(s) 199–209

    Abstract: Objective: Whole genome sequencing (WGS) of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E. coli) in developing countries is lacking. Here we describe the population structure and molecular characteristics of ESBL-E. coli faecal ... ...

    Abstract Objective: Whole genome sequencing (WGS) of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E. coli) in developing countries is lacking. Here we describe the population structure and molecular characteristics of ESBL-E. coli faecal isolates in rural Southern Niger.
    Methods: Stools of 383 healthy participants were collected among which 92.4% were ESBL-Enterobacterales carriers. A subset of 90 ESBL-E. coli containing stools (109 ESBL-E. coli isolates) were further analysed by WGS, using short- and long-reads.
    Results: Most isolates belonged to the commensalism-adapted phylogroup A (83.5%), with high clonal diversity. The bla
    Conclusions: Massive prevalence of community faecal carriage of CTX-M-15-producing E. coli was observed in a rural region of Niger due to the spread of highly diverse A phylogroup commensalism-adapted clones, with frequent chromosomal integration of bla
    MeSH term(s) Humans ; Escherichia coli/genetics ; Escherichia coli Infections/epidemiology ; Niger/epidemiology ; Anti-Bacterial Agents ; beta-Lactamases/genetics ; Plasmids/genetics
    Chemical Substances Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2023-06-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2023.06.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A guide to aid the selection of diagnostic tests.

    Kosack, Cara S / Page, Anne-Laure / Klatser, Paul R

    Bulletin of the World Health Organization

    2017  Volume 95, Issue 9, Page(s) 639–645

    Abstract: In recent years, a wide range of diagnostic tests has become available for use in resource-constrained settings. Accordingly, a huge number of guidelines, performance evaluations and implementation reports have been produced. However, this wealth of ... ...

    Abstract In recent years, a wide range of diagnostic tests has become available for use in resource-constrained settings. Accordingly, a huge number of guidelines, performance evaluations and implementation reports have been produced. However, this wealth of information is unstructured and of uneven quality, which has made it difficult for end-users, such as clinics, laboratories and health ministries, to determine which test would be best for improving clinical care and patient outcomes in a specific context. This paper outlines a six-step guide to the selection and implementation of in vitro diagnostic tests based on Médecins Sans Frontières' practical experience: (i) define the test's purpose; (ii) review the market; (iii) ascertain regulatory approval; (iv) determine the test's diagnostic accuracy under ideal conditions; (v) determine the test's diagnostic accuracy in clinical practice; and (vi) monitor the test's performance in routine use. Gaps in the information needed to complete these six steps and gaps in regulatory systems are highlighted. Finally, ways of improving the quality of diagnostic tests are suggested, such as establishing a model list of essential diagnostics, establishing a repository of information on the design of diagnostic studies and improving quality control and postmarketing surveillance.
    MeSH term(s) Communicable Diseases/diagnosis ; Diagnostic Tests, Routine/methods ; Diagnostic Tests, Routine/standards ; Humans ; Practice Guidelines as Topic ; Reagent Kits, Diagnostic/standards ; United States ; United States Food and Drug Administration ; World Health Organization
    Chemical Substances Reagent Kits, Diagnostic
    Language English
    Publishing date 2017-09-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 80213-x
    ISSN 1564-0604 ; 0042-9686 ; 0366-4996 ; 0510-8659
    ISSN (online) 1564-0604
    ISSN 0042-9686 ; 0366-4996 ; 0510-8659
    DOI 10.2471/BLT.16.187468
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Light-emitting diode fluorescence microscopy for tuberculosis diagnosis: a meta-analysis.

    Chang, Eva W / Page, Anne-Laure / Bonnet, Maryline

    The European respiratory journal

    2016  Volume 47, Issue 3, Page(s) 929–937

    Abstract: Light-emitting diode fluorescence microscopy (LED-FM) is recommended by the World Health Organization to replace conventional Ziehl-Neelsen microscopy for pulmonary tuberculosis diagnosis. Uptake of LED-FM has been slow. One reason is its reported loss ... ...

    Abstract Light-emitting diode fluorescence microscopy (LED-FM) is recommended by the World Health Organization to replace conventional Ziehl-Neelsen microscopy for pulmonary tuberculosis diagnosis. Uptake of LED-FM has been slow. One reason is its reported loss of specificity compared with Ziehl-Neelsen microscopy. We aimed to determine the diagnostic accuracy of LED-FM for tuberculosis detection and explore potential factors that might affect its performance.A comprehensive search strategy based on pre-specified criteria was employed to identify eligible studies between January 1, 2000 and April 1, 2014 in 11 databases. Standardised study selection, data extraction and quality assessment were conducted. Pooled sensitivity and specificity of LED-FM using culture as the reference standard were estimated through meta-analyses using a bivariate random-effects model. Investigation of heterogeneity was performed by subgroup analyses.We identified 12 unique studies, half of which were from peripheral healthcare facilities. LED-FM achieved a pooled sensitivity of 66.9% (95% CI 60.5-72.7%) and pooled specificity of 96.8% (95% CI 93.1-98.6%). A pooled sensitivity of 53.0% (95% CI 42.8-63.0%) and pooled specificity of 96.1% (95% CI 86.0-99.0%) were obtained by LED-FM among HIV-infected patients. Study methodology factors and differences in the LED-FM procedure or device could also affect the performance.LED-FM specificity is high and should not be a barrier to device introduction, particularly among peripheral healthcare settings where this technology is meant to be used. Sensitivity is reduced in HIV-infected patients.
    MeSH term(s) HIV Infections/complications ; HIV Infections/microbiology ; Humans ; Microscopy, Fluorescence/standards ; Mycobacterium tuberculosis/isolation & purification ; Sensitivity and Specificity ; Sputum/microbiology ; Tuberculosis, Pulmonary/diagnosis ; World Health Organization
    Language English
    Publishing date 2016-03
    Publishing country England
    Document type Journal Article ; Meta-Analysis ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 639359-7
    ISSN 1399-3003 ; 0903-1936
    ISSN (online) 1399-3003
    ISSN 0903-1936
    DOI 10.1183/13993003.00978-2015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Journal ; Article ; Online: A guide to aid the selection of diagnostic tests

    Kosack, Cara S / Page, Anne-Laure / Klatser, Paul R

    2017  

    Abstract: ... 639 ... ...

    Abstract 639

    645
    Keywords Policy and Practice
    Publisher World Health Organization
    Document type Journal ; Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Switching from natalizumab administration at the day hospital to administration at home. A 1 year prospective study of patient experience and quality of life in 30 consecutive patients with multiple sclerosis (TYSAD-35).

    Lamy, Simon / Veillard, David / Doyen, Hélène / Kerbrat, Anne / Michel, Laure / Chretien, Emilie / Ousmen, Ahmad / Edan, Gilles / Le Page, Emmanuelle

    Multiple sclerosis and related disorders

    2023  Volume 73, Page(s) 104657

    Abstract: Background: In the context of the COVID-19 pandemic, French health authorities allowed the home administration of natalizumab by a healthcare-at-home service. We evaluated the patients' perception of care quality following the transition from day- ... ...

    Abstract Background: In the context of the COVID-19 pandemic, French health authorities allowed the home administration of natalizumab by a healthcare-at-home service. We evaluated the patients' perception of care quality following the transition from day-hospital to home natalizumab administration.
    Methods: Thirty relapsing-remitting multiple sclerosis (MS) patients treated with natalizumab were prospectively evaluated for one year after changing onto a home treatment procedure, using MusiCare, the first MS-specific questionnaire to evaluate patient experience and MusiQol. A numerical rating scale score for satisfaction and a dedicated questionnaire concerning patient experience were completed after each infusion. The primary endpoint was the mean difference in MusiCare score between baseline and 12 months.
    Results: From June 2020 to November 2021, 306 infusions were performed at home. Three patients withdrew from the study (one lost to follow-up and two preferred to return at the day hospital). No worsening of patient experience or quality of life was observed. The mean scores of the Musicare dimensions were higher at 12 months than at baseline, significantly for the "relationship with healthcare professionals" (p = 0.0203). The MusiQol global score remained stable but the coping and friendship dimensions were significantly better at M12 than at baseline (p = 0.0491 and p = 0.0478, respectively). The satisfaction questionnaire highlighted some pain during the infusions (21.8%) and contradictions between healthcare professionals (17.2%). The mean score for satisfaction with care was 9.1/10. No safety concerns were identified.
    Conclusion: The positive experience of patients with home natalizumab administration provides an important opportunity to improve the quality of patient care.
    MeSH term(s) Humans ; Natalizumab/adverse effects ; Multiple Sclerosis/drug therapy ; Multiple Sclerosis/epidemiology ; Prospective Studies ; Immunologic Factors/adverse effects ; Quality of Life ; Pandemics ; COVID-19 ; Multiple Sclerosis, Relapsing-Remitting/drug therapy ; Patient Outcome Assessment ; Hospitals
    Chemical Substances Natalizumab ; Immunologic Factors
    Language English
    Publishing date 2023-03-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2645330-7
    ISSN 2211-0356 ; 2211-0348
    ISSN (online) 2211-0356
    ISSN 2211-0348
    DOI 10.1016/j.msard.2023.104657
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders.

    Maitre, Elsa / Le-Page, Anne-Laure / Comoz, Francois / Truquet, Florence / Damaj, Gandhi / Cornet, Edouard / Verneuil, Laurence / Salaün, Véronique / Troussard, Xavier

    Cytometry. Part B, Clinical cytometry

    2019  Volume 96, Issue 4, Page(s) 283–293

    Abstract: Background: Histological evaluation of malignant hematologic involvement of the skin can be challenging and needs an extended immunohistochemistry panel. We assessed the ability of flow cytometry (FCM) to detect neoplastic cell subsets in skin biopsies ... ...

    Abstract Background: Histological evaluation of malignant hematologic involvement of the skin can be challenging and needs an extended immunohistochemistry panel. We assessed the ability of flow cytometry (FCM) to detect neoplastic cell subsets in skin biopsies as a useful tool that supplements the histological examination in a complementary way.
    Methods: Two hundred and forty-three consecutive skin biopsies were retrospectively analyzed between April 2012 and July 2017.
    Results: Among them, 147 samples, corresponding to 128 patients, were analyzed at diagnosis. Eighty-seven patients had erythrodermic inflammatory dermatoses, and 41 patients had cutaneous hematologic neoplasms. Cutaneous T-cell lymphomas were the most frequent disorders, accounting for 70% of cases (29/41). Cutaneous B-cell lymphoma was found in only 17% of cases (7/41) and immature hematologic malignancies in 5% (2/41). Three patients had secondary skin involvement. The sensitivity of FCM skin biopsy analysis was 78.1% (32/41). Among the 243 samples, 27 patients had mycosis fungoides (MF) or Sezary syndrome (SS) with available FCM data. A loss of CD26 expression was identified in 92% of cases of transformed MF or SS versus 40% of cases of non-transformed MF (P = 0.0057 χ²). Among the 12 MF patients with negative CD26 expression, six progressed to SS versus none in the positive group (50% vs. 0% P = 0.0168 χ²).
    Conclusions: FCM analysis of the skin biopsies is a sensitive method and a useful tool for improving the sensitivity of diagnosis of hematologic skin neoplasms. Among the MF patients, a loss of CD26 expression could be a marker of higher risk of progression. © 2019 International Clinical Cytometry Society.
    MeSH term(s) Aged ; Cohort Studies ; Dipeptidyl Peptidase 4/genetics ; Female ; Flow Cytometry ; Hematologic Neoplasms/diagnosis ; Hematologic Neoplasms/genetics ; Humans ; Male ; Middle Aged ; Myeloproliferative Disorders/diagnosis ; Myeloproliferative Disorders/genetics ; Skin Neoplasms/diagnosis ; Skin Neoplasms/genetics
    Chemical Substances Dipeptidyl Peptidase 4 (EC 3.4.14.5)
    Language English
    Publishing date 2019-05-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2099657-3
    ISSN 1552-4957 ; 1552-4949 ; 0196-4763
    ISSN (online) 1552-4957
    ISSN 1552-4949 ; 0196-4763
    DOI 10.1002/cyto.b.21784
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Prevalence and risk factors of brucellosis among febrile patients attending a community hospital in south western Uganda

    Richard Migisha / Dan Nyehangane / Yap Boum / Anne-Laure Page / Amaia Zúñiga-Ripa / Raquel Conde-Álvarez / Fred Bagenda / Maryline Bonnet

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 8

    Abstract: Abstract Human brucellosis, a chronic disease contracted through contact with animals and consuption of unpasteurized dairy products is underreported in limited-resource countries. This cross-sectional study aimed to determine the prevalence and risk ... ...

    Abstract Abstract Human brucellosis, a chronic disease contracted through contact with animals and consuption of unpasteurized dairy products is underreported in limited-resource countries. This cross-sectional study aimed to determine the prevalence and risk factors of brucellosis among febrile patients attending a community hospital in South western Uganda. A questionnaire that captured socio-demographic, occupational and clinical data was administered. Blood samples were tested for Brucella antibodies using Rose Bengal Plate Test (RBPT) and blood culture with standard aerobic BACTEC bottle was done. Of 235 patients enrolled, prevalence of brucellosis (RBPT or culture confirmed) was 14.9% (95% CI 10.6–20.1) with a culture confrmation in 4.3% of the participants. The factors independently associated with brucellosis were consumption of raw milk (aOR 406.15, 95% CI 47.67–3461.69); history of brucellosis in the family (aOR 9.19, 95% CI 1.98–42.54); and selling hides and skins (aOR 162.56, 95% CI 2.86–9256.31). Hepatomegaly (p < 0.001), splenomegaly (p = 0.018) and low body mass index (p = 0.032) were more common in patients with brucellosis compared to others. Our findings reveal a high prevalence of brucellosis among febrile patients and highlight a need for implementing appropiate tests, public awareness activities and vaccination of animals to control and eliminate the disease.
    Keywords Medicine ; R ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2018-10-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Discontinuation of second- versus first-line disease-modifying treatment in middle-aged patients with multiple sclerosis.

    Chappuis, Maëlle / Rousseau, Chloé / Bajeux, Emma / Wiertlewski, Sandrine / Laplaud, David / Le Page, Emmanuelle / Michel, Laure / Edan, Gilles / Kerbrat, Anne

    Journal of neurology

    2022  

    Abstract: Background and purpose: There has been scant research on the consequences of discontinuing second-line disease-modifying treatment (DMT) in middle-aged patients with multiple sclerosis (MS). The objective was therefore to examine the occurrence of focal ...

    Abstract Background and purpose: There has been scant research on the consequences of discontinuing second-line disease-modifying treatment (DMT) in middle-aged patients with multiple sclerosis (MS). The objective was therefore to examine the occurrence of focal inflammatory activity after the discontinuation of second versus first-line DMT in patients over 45 years.
    Methods: Patients who had been treated for at least 6 months with second (natalizumab, fingolimod, anti CD20) or first-line DMT and who stopped their DMT were retrospectively included. Kaplan-Meier survival curves were used to study the occurrence of relapse and MRI activity according to the type of DMT stopped. Proportional hazard Cox models were calculated to identify factors associated with focal inflammatory activity. The annualized relapse rate was calculated under treatment and for every 3 months after DMT discontinuation.
    Results: We included 232 patients (median age: 52.8 years), 49 of whom stopped second-line DMT. The probability of having a relapse within the year following discontinuation was 6% for first-line DMT, 9% for fingolimod and 43% for natalizumab. In multivariate analysis, the probability of relapse after DMT discontinuation was significantly increased with natalizumab compared to first-line DMT (HR = 3.24; 95% CI [1.52; 6.90]). A peak of relapse was observed at 0-3 months after stopping natalizumab or fingolimod.
    Conclusion: Our study suggests that the risk of inflammatory activity is greater after discontinuation of natalizumab compared to other DMT even in middle-aged patients. As for younger patients, natalizumab discontinuation should only be considered if there is an adequate substitution of a different therapy. .
    Language English
    Publishing date 2022-09-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 187050-6
    ISSN 1432-1459 ; 0340-5354 ; 0012-1037 ; 0939-1517 ; 1619-800X
    ISSN (online) 1432-1459
    ISSN 0340-5354 ; 0012-1037 ; 0939-1517 ; 1619-800X
    DOI 10.1007/s00415-022-11341-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Prevalence and risk factors of brucellosis among febrile patients attending a community hospital in south western Uganda.

    Migisha, Richard / Dan Nyehangane / Boum, Yap / Page, Anne-Laure / Zúñiga-Ripa, Amaia / Conde-Álvarez, Raquel / Bagenda, Fred / Bonnet, Maryline

    Scientific reports

    2018  Volume 8, Issue 1, Page(s) 15465

    Abstract: Human brucellosis, a chronic disease contracted through contact with animals and consuption of unpasteurized dairy products is underreported in limited-resource countries. This cross-sectional study aimed to determine the prevalence and risk factors of ... ...

    Abstract Human brucellosis, a chronic disease contracted through contact with animals and consuption of unpasteurized dairy products is underreported in limited-resource countries. This cross-sectional study aimed to determine the prevalence and risk factors of brucellosis among febrile patients attending a community hospital in South western Uganda. A questionnaire that captured socio-demographic, occupational and clinical data was administered. Blood samples were tested for Brucella antibodies using Rose Bengal Plate Test (RBPT) and blood culture with standard aerobic BACTEC bottle was done. Of 235 patients enrolled, prevalence of brucellosis (RBPT or culture confirmed) was 14.9% (95% CI 10.6-20.1) with a culture confrmation in 4.3% of the participants. The factors independently associated with brucellosis were consumption of raw milk (aOR 406.15, 95% CI 47.67-3461.69); history of brucellosis in the family (aOR 9.19, 95% CI 1.98-42.54); and selling hides and skins (aOR 162.56, 95% CI 2.86-9256.31). Hepatomegaly (p < 0.001), splenomegaly (p = 0.018) and low body mass index (p = 0.032) were more common in patients with brucellosis compared to others. Our findings reveal a high prevalence of brucellosis among febrile patients and highlight a need for implementing appropiate tests, public awareness activities and vaccination of animals to control and eliminate the disease.
    MeSH term(s) Adult ; Brucella/isolation & purification ; Brucellosis/epidemiology ; Brucellosis/microbiology ; Brucellosis/transmission ; Cross-Sectional Studies ; Dairy Products ; Female ; Fever/physiopathology ; Hospitals, Community/statistics & numerical data ; Humans ; Male ; Middle Aged ; Prevalence ; Risk Factors ; Uganda/epidemiology ; Young Adult
    Language English
    Publishing date 2018-10-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-018-33915-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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