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  1. Article ; Online: Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes.

    Nicolay, Wiebke / Moeller, Rebecca / Kahl, Sina / Vondran, Florian W R / Pietschmann, Thomas / Kunz, Stefan / Gerold, Gisa

    Cells

    2021  Volume 10, Issue 11

    Abstract: The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly ... ...

    Abstract The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes.
    MeSH term(s) Carcinoma, Hepatocellular/pathology ; Cell Line, Tumor ; Cells, Cultured ; DEAD Box Protein 58/immunology ; Hepatocytes/immunology ; Hepatocytes/virology ; Humans ; Immunity, Innate ; Interferons/immunology ; Liver/cytology ; Liver/immunology ; Liver/virology ; Liver Neoplasms/pathology ; RNA/immunology ; RNA Viruses/physiology ; Receptors, Immunologic/immunology ; Signal Transduction/immunology ; Toll-Like Receptor 3/immunology ; Viral Load
    Chemical Substances Receptors, Immunologic ; TLR3 protein, human ; Toll-Like Receptor 3 ; RNA (63231-63-0) ; Interferons (9008-11-1) ; RIGI protein, human (EC 3.6.1.-) ; DEAD Box Protein 58 (EC 3.6.4.13)
    Language English
    Publishing date 2021-11-04
    Publishing country Switzerland
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10113019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes

    Wiebke Nicolay / Rebecca Moeller / Sina Kahl / Florian W. R. Vondran / Thomas Pietschmann / Stefan Kunz / Gisa Gerold

    Cells, Vol 10, Iss 3019, p

    2021  Volume 3019

    Abstract: The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly ... ...

    Abstract The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes.
    Keywords hepatoma cells ; primary hepatocytes ; liver ; RNA virus ; innate immunity ; RIG-I ; Biology (General) ; QH301-705.5
    Subject code 610
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  3. Article ; Online: Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes.

    Nicolay, Wiebke / Moeller, Rebecca / Kahl, Sina / Vondran, Florian W R / Pietschmann, Thomas / Kunz, Stefan / Gerold, Gisa

    10 ; 11 ; Cells ; Switzerland

    2021  

    Abstract: The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly ... ...

    Abstract The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes.
    Keywords RIG-I ; RNA virus ; TLR3 ; arenavirus ; coronavirus ; hepatoma cells ; innate immunity ; interferon ; liver ; primary hepatocytes
    Subject code 610
    Language English
    Publishing date 2021-11-04
    Publisher MDPI
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article: In situ measurements of tropical cloud properties in the West African Monsoon: upper tropospheric ice clouds, Mesoscale Convective System outflow, and subvisual cirrus

    Frey, Wiebke / Borrmann, Stephan / Kunkel, Daniel / Weigel, Ralf / Reus, Marian de / Schlager, Hans / Roiger, Anke / Voigt, Christiane / Hoor, Peter / Curtius, Joachim / Krämer, M. / Schiller, Cornelius / Volk, C.-Michael / Homan, Carine Dorianne / Fierli, Federico / Di Donfrancesco, Guido / Ulanovsky, Alexey / Ravegnani, Fabrizio / Sitnikov, Nicolay M. /
    Viciani, Silvia / D'Amato, F. / Shur, Genrikh N. / Belyaev, Gennady / Law, Kathy S. / Cairo, Francesco

    Abstract: In situ measurements of ice crystal size distributions in tropical upper troposphere/lower stratosphere (UT/LS) clouds were performed during the SCOUT-AMMA campaign over West Africa in August 2006. The cloud properties were measured with a Forward ... ...

    Abstract In situ measurements of ice crystal size distributions in tropical upper troposphere/lower stratosphere (UT/LS) clouds were performed during the SCOUT-AMMA campaign over West Africa in August 2006. The cloud properties were measured with a Forward Scattering Spectrometer Probe (FSSP-100) and a Cloud Imaging Probe (CIP) operated aboard the Russian high altitude research aircraft M-55 Geophysica with the mission base in Ouagadougou, Burkina Faso. A total of 117 ice particle size distributions were obtained from the measurements in the vicinity of Mesoscale Convective Systems (MCS). Two to four modal lognormal size distributions were fitted to the average size distributions for different potential temperature bins. The measurements showed proportionately more large ice particles compared to former measurements above maritime regions. With the help of trace gas measurements of NO, NOy, CO2, CO, and O3 and satellite images, clouds in young and aged MCS outflow were identified. These events were observed at altitudes of 11.0 km to 14.2 km corresponding to potential temperature levels of 346 K to 356 K. In a young outflow from a developing MCS ice crystal number concentrations of up to (8.3 ± 1.6) cm−3 and rimed ice particles with maximum dimensions exceeding 1.5 mm were found. A maximum ice water content of 0.05 g m−3 was observed and an effective radius of about 90 μm. In contrast the aged outflow events were more diluted and showed a maximum number concentration of 0.03 cm−3, an ice water content of 2.3 × 10−4 g m−3, an effective radius of about 18 μm, while the largest particles had a maximum dimension of 61 μm.Close to the tropopause subvisual cirrus were encountered four times at altitudes of 15 km to 16.4 km. The mean ice particle number concentration of these encounters was 0.01 cm−3 with maximum particle sizes of 130 μm, and the mean ice water content was about 1.4 × 10−4 g m−3. All known in situ measurements of subvisual tropopause cirrus are compared and an exponential fit on the size distributions is established for modelling purposes.A comparison of aerosol to ice crystal number concentrations, in order to obtain an estimate on how many ice particles may result from activation of the present aerosol, yielded low ratios for the subvisual cirrus cases of roughly one cloud particle per 30 000 aerosol particles, while for the MCS outflow cases this resulted in a high ratio of one cloud particle per 300 aerosol particles.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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