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Article: Schweinegrippe: Interview mit Thorsten Wolff, RKI Berlin

Wolff, Thorsten

2010 Volume 17, Issue 3, Page(s) 40

Language	German
Document type	Article
ZDB-ID	1237282-1
ISSN	1612-8354
Database	Current Contents Medicine

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Article ; Online: Efficient supply with carbon dioxide from flue gas during large scale production of microalgae: A novel approach for bioenergy facades.

Kerner, Martin / Wolff, Thorsten / Brinkmann, Torsten

Bioresource technology

2023 Volume 391, Issue Pt A, Page(s) 129917

Abstract: The efficiency of using enriched ... ...

Abstract	The efficiency of using enriched CO
MeSH term(s)	Carbon Dioxide ; Microalgae ; Chlorella ; Culture Media ; Biomass
Chemical Substances	Carbon Dioxide (142M471B3J) ; Culture Media
Language	English
Publishing date	2023-10-24
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2023.129917
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Editorial: Emergency medicine in pediatric patients with chronic diseases.

Schneck, Emmanuel / Wolff, Johannes / Graesner, Jan-Thorsten / Hauch, Holger

Frontiers in pediatrics

2023 Volume 11, Page(s) 1185636

Language	English
Publishing date	2023-03-30
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2711999-3
ISSN	2296-2360
ISSN	2296-2360
DOI	10.3389/fped.2023.1185636
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Article ; Online: The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.

Krischuns, Tim / Arragain, Benoît / Isel, Catherine / Paisant, Sylvain / Budt, Matthias / Wolff, Thorsten / Cusack, Stephen / Naffakh, Nadia

Nature communications

2024 Volume 15, Issue 1, Page(s) 1064

Abstract: The current model is that the influenza virus polymerase (FluPol) binds either to host RNA polymerase II (RNAP II) or to the acidic nuclear phosphoprotein 32 (ANP32), which drives its conformation and activity towards transcription or replication of the ... ...

Abstract	The current model is that the influenza virus polymerase (FluPol) binds either to host RNA polymerase II (RNAP II) or to the acidic nuclear phosphoprotein 32 (ANP32), which drives its conformation and activity towards transcription or replication of the viral genome, respectively. Here, we provide evidence that the FluPol-RNAP II binding interface, beyond its well-acknowledged function in cap-snatching during transcription initiation, has also a pivotal role in replication of the viral genome. Using a combination of cell-based and in vitro approaches, we show that the RNAP II C-terminal-domain, jointly with ANP32, enhances FluPol replication activity. We observe successive conformational changes to switch from a transcriptase to a replicase conformation in the presence of the bound RNPAII C-terminal domain and propose a model in which the host RNAP II is the anchor for transcription and replication of the viral genome. Our data open new perspectives on the spatial coupling of viral transcription and replication and the coordinated balance between these two activities.
MeSH term(s)	RNA Polymerase II/metabolism ; RNA-Dependent RNA Polymerase/metabolism ; RNA, Viral/genetics ; Orthomyxoviridae/genetics ; DNA-Directed RNA Polymerases ; Virus Replication/genetics
Chemical Substances	RNA Polymerase II (EC 2.7.7.-) ; RNA-Dependent RNA Polymerase (EC 2.7.7.48) ; RNA, Viral ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
Language	English
Publishing date	2024-02-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45205-2
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Article ; Online: Characteristics of two zoonotic swine influenza A(H1N1) viruses isolated in Germany from diseased patients.

Heider, Alla / Wedde, Marianne / Weinheimer, Viola / Döllinger, Stephanie / Monazahian, Masyar / Dürrwald, Ralf / Wolff, Thorsten / Schweiger, Brunhilde

International journal of medical microbiology : IJMM

2024 Volume 314, Page(s) 151609

Abstract: Interspecies transmission of influenza A viruses (IAV) from pigs to humans is a concerning event as porcine IAV represent a reservoir of potentially pandemic IAV. We conducted a comprehensive analysis of two porcine A(H1N1)v viruses isolated from human ... ...

Abstract	Interspecies transmission of influenza A viruses (IAV) from pigs to humans is a concerning event as porcine IAV represent a reservoir of potentially pandemic IAV. We conducted a comprehensive analysis of two porcine A(H1N1)v viruses isolated from human cases by evaluating their genetic, antigenic and virological characteristics. The HA genes of those human isolates belonged to clades 1C.2.1 and 1C.2.2, respectively, of the A(H1N1) Eurasian avian-like swine influenza lineage. Antigenic profiling revealed substantial cross-reactivity between the two zoonotic H1N1 viruses and human A(H1N1)pdm09 virus and some swine viruses, but did not reveal cross-reactivity to H1N2 and earlier human seasonal A(H1N1) viruses. The solid-phase direct receptor binding assay analysis of both A(H1N1)v showed a predominant binding to α2-6-sialylated glycans similar to human-adapted IAV. Investigation of the replicative potential revealed that both A(H1N1)v viruses grow in human bronchial epithelial cells to similar high titers as the human A(H1N1)pdm09 virus. Cytokine induction was studied in human alveolar epithelial cells A549 and showed that both swine viruses isolated from human cases induced higher amounts of type I and type III IFN, as well as IL6 compared to a seasonal A(H1N1) or a A(H1N1)pdm09 virus. In summary, we demonstrate a remarkable adaptation of both zoonotic viruses to propagate in human cells. Our data emphasize the needs for continuous monitoring of people and regions at increased risk of such trans-species transmissions, as well as systematic studies to quantify the frequency of these events and to identify viral molecular determinants enhancing the zoonotic potential of porcine IAV.
MeSH term(s)	Humans ; Animals ; Swine ; Influenza A Virus, H1N1 Subtype/genetics ; Orthomyxoviridae Infections/epidemiology ; Orthomyxoviridae Infections/veterinary ; Influenza, Human/epidemiology ; Germany/epidemiology ; Swine Diseases/epidemiology ; Phylogeny
Language	English
Publishing date	2024-01-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2006518-8
ISSN	1618-0607 ; 1438-4221
ISSN (online)	1618-0607
ISSN	1438-4221
DOI	10.1016/j.ijmm.2024.151609
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Article ; Online: Argon Plasma Coagulation of Gastric Inlet Patches of the Cervical Esophagus Relieves Vocal and Respiratory Symptoms in Selected Patients.

Brechmann, Thorsten / Mühlenkamp, Maximilian / Schmiegel, Wolff / Viebahn, Bernd

Digestive diseases and sciences

2022 Volume 68, Issue 5, Page(s) 1936–1943

Abstract: Objectives: Argon plasma coagulation (APC) of gastric inlet patches of the cervical esophagus (GIP) has been shown to relieve the globus sensation. This study aimed to investigate the long-term effects of APC therapy on a variety of laryngopharyngeal ... ...

Abstract	Objectives: Argon plasma coagulation (APC) of gastric inlet patches of the cervical esophagus (GIP) has been shown to relieve the globus sensation. This study aimed to investigate the long-term effects of APC therapy on a variety of laryngopharyngeal symptoms. Methods: Patients with laryngopharyngeal symptoms who had undergone endoscopic GIP eradication via APC therapy were eligible for a retrospective observational cohort study. Symptom relief was assessed by a five-grade scale during a structured interview. Statistical analysis included ANOVA, the chi-squared and t-test. Results: A total of 45 patients (61.0 (52.0; 69.0) years, 26 (57.8%) female) were included after a follow-up time of 85.0 (55.3; 111.0) months. Symptoms included dysphagia (56.0%), dysphonia (51.0%), hoarseness (76.0%), the necessity of throat clearing (73.0%), globus sensation (56.0%) and heartburn (71.0%). Complete GIP eradication was achieved after one session in 25 (55.6%), repetitive sessions were needed in the remaining cases. Fourteen patients (31.1%) reported a very strong, 11 (24.4%) a strong and 8 patients (17.8%) an intermediate improvement; five patients did not benefit. The most apparent improvement was seen regarding hoarseness (from 2.6 ± 1.7 to 1.2 ± 1.3), the necessity of throat clearing (from 2.6 ± 1.7 to 1.1 ± 1.3), globus sensation (from 2.0 ± 1.9 to 0.9 ± 1.3) and heartburn (from 2.5 ± 1.8 to 1.4 ± 1.6). Adverse events included post-endoscopic pain (n = 6, 13.3%) and purulent pharyngitis (n = 2, 4.4%). Conclusions: The APC therapy of GIP is safe and leads to long-term relief of laryngopharyngeal symptoms in carefully selected patients.
MeSH term(s)	Humans ; Female ; Male ; Heartburn/diagnosis ; Argon Plasma Coagulation ; Hoarseness ; Bays ; Retrospective Studies ; Gastric Mucosa ; Prospective Studies ; Esophagus/surgery
Language	English
Publishing date	2022-11-14
Publishing country	United States
Document type	Observational Study ; Journal Article
ZDB-ID	304250-9
ISSN	1573-2568 ; 0163-2116
ISSN (online)	1573-2568
ISSN	0163-2116
DOI	10.1007/s10620-022-07745-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Surveillance of endemic human Coronaviruses in Germany, 2019/2020.

Biere, Barbara / Oh, Djin-Ye / Wolff, Thorsten / Dürrwald, Ralf

The Lancet regional health. Europe

2021 Volume 11, Page(s) 100262

Language	English
Publishing date	2021-11-04
Publishing country	England
Document type	Journal Article
ISSN	2666-7762
ISSN (online)	2666-7762
DOI	10.1016/j.lanepe.2021.100262
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Article ; Online: Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome.

Fu, Yuguang / Wedde, Marianne / Smola, Sigrun / Oh, Djin-Ye / Pfuhl, Thorsten / Rissland, Jürgen / Zemlin, Michael / Flockerzi, Fidelis A / Bohle, Rainer M / Thürmer, Andrea / Duwe, Susanne / Biere, Barbara / Reiche, Janine / Schweiger, Brunhilde / Mache, Christin / Wolff, Thorsten / Herrler, Georg / Dürrwald, Ralf

International journal of medical microbiology : IJMM

2024 Volume 314, Page(s) 151598

Abstract: Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized. A patient (age group 5-15 years) displaying symptoms of hemolytic uremic ... ...

Abstract	Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized. A patient (age group 5-15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined. Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed. The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
MeSH term(s)	Humans ; Child, Preschool ; Child ; Adolescent ; Influenza A Virus, H1N1 Subtype ; Epithelial Cells ; Lung ; Hemolytic-Uremic Syndrome ; Influenza, Human/epidemiology
Language	English
Publishing date	2024-01-11
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2006518-8
ISSN	1618-0607 ; 1438-4221
ISSN (online)	1618-0607
ISSN	1438-4221
DOI	10.1016/j.ijmm.2024.151598
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Article ; Online: Molecular characterization and evolution dynamics of influenza B viruses circulating in Germany from season 1996/1997 to 2019/2020.

Heider, Alla / Wedde, Marianne / Dürrwald, Ralf / Wolff, Thorsten / Schweiger, Brunhilde

Virus research

2022 Volume 322, Page(s) 198926

Abstract: Influenza B viruses are responsible for significant disease burden caused by viruses of both the Yamagata- and Victoria-lineage. Since the circulating patterns of influenza B viruses in different countries vary we investigated molecular properties and ... ...

Abstract	Influenza B viruses are responsible for significant disease burden caused by viruses of both the Yamagata- and Victoria-lineage. Since the circulating patterns of influenza B viruses in different countries vary we investigated molecular properties and evolution dynamics of influenza B viruses circulating in Germany between 1996 and 2020. A change of the dominant lineage occurred in Germany in seven seasons in over past 25 years. A total of 676 sequences of hemagglutinin coding domain 1 (HA1) and 516 sequences of neuraminidase (NA) genes of Yamagata- and Victoria-lineage viruses were analyzed using time-scaled phylogenetic tree. Phylogenetic analysis demonstrated that Yamagata-lineage viruses are more diverse than the Victoria-lineage viruses and could be divided into nine genetic groups whereas Victoria-lineage viruses presented six genetic groups. Comparative phylogenetic analyses of both the HA and NA segments together revealed a number of inter-lineage as well as inter- and intra-clade reassortants. We identified key amino acid substitutions in major HA epitopes such as in four antigenic sites and receptor-binding sites (RBS) and in the regions close to them, with most substitutions in the 120-loop of both lineage viruses. Altogether, seventeen substitutions were fixed over time within the Yamagata-lineage with twelve of them in the antigenic sites. Thirteen substitutions were identified within the Victoria-lineage, with eleven of them in the antigenic sites. Moreover, all Victoria-lineage viruses of the 2017/2018 season were characterized by a deletion of two amino acids at the position 162-163 in the antigenic site of HA1. The viruses with triple deletion Δ162-164 were found in Germany since season 2018/2019. We highlighted the interplay between substitutions in the glycosylation sites and RBS and antigenic epitope during HA evolution. The results obtained underscore the need for continuous monitoring of circulating influenza B viruses. Early detection of strains with genetic and antigenic variation is essential to predict the circulation patterns for the following season. Such information is important for the development of optimal vaccines and strategies for prevention and control of influenza.
MeSH term(s)	Humans ; Influenza B virus/genetics ; Seasons ; Phylogeny ; Hemagglutinin Glycoproteins, Influenza Virus/genetics ; Influenza, Human ; Neuraminidase/genetics ; Epitopes ; Evolution, Molecular
Chemical Substances	Hemagglutinin Glycoproteins, Influenza Virus ; Neuraminidase (EC 3.2.1.18) ; Epitopes
Language	English
Publishing date	2022-09-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605780-9
ISSN	1872-7492 ; 0168-1702
ISSN (online)	1872-7492
ISSN	0168-1702
DOI	10.1016/j.virusres.2022.198926
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Article ; Online: Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome

Yuguang Fu / Marianne Wedde / Sigrun Smola / Djin-Ye Oh / Thorsten Pfuhl / Jürgen Rissland / Michael Zemlin / Fidelis A. Flockerzi / Rainer M. Bohle / Andrea Thürmer / Susanne Duwe / Barbara Biere / Janine Reiche / Brunhilde Schweiger / Christin Mache / Thorsten Wolff / Georg Herrler / Ralf Dürrwald

International Journal of Medical Microbiology, Vol 314, Iss , Pp 151598- (2024)

2024

Abstract: Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic ... ...

Abstract	Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
Keywords	A(H1N1)pdm09 virus ; Fatal influenza ; S263S/F (HA1) and H456H/Y (PB1) mutations ; Microbiology ; QR1-502 ; Other systems of medicine ; RZ201-999
Language	English
Publishing date	2024-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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