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  1. Article: Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform.

    Kimpston-Burkgren, Kay / Mora-Díaz, Juan Carlos / Roby, Philippe / Bjustrom-Kraft, Jordan / Main, Rodger / Bosse, Roger / Giménez-Lirola, Luis Gabriel

    Pathogens (Basel, Switzerland)

    2020  Volume 9, Issue 3

    Abstract: Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging ... ...

    Abstract Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic
    Keywords covid19
    Language English
    Publishing date 2020-03-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens9030233
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform

    Kay Kimpston-Burkgren / Juan Carlos Mora-Díaz / Philippe Roby / Jordan Bjustrom-Kraft / Rodger Main / Roger Bosse / Luis Gabriel Giménez-Lirola

    Pathogens, Vol 9, Iss 3, p

    2020  Volume 233

    Abstract: Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging ... ...

    Abstract Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alphacoronavirus PED virus (PEDV). In the absence of effective vaccines, infection prevention and control through diagnostic testing and quarantine are critical. Early detection and differential diagnosis of PEDV infections increase the chance of successful control of the disease. Therefore, there is a continuous need for development of reduced assay-step protocols, no-wash, high-throughput immunoassays. This study described the characterization of the humoral immune response against PEDV under experimental and field conditions using a rapid, sensitive, luminescent proximity homogenous assay (AlphaLISA). PEDV IgG and IgA antibodies were developed toward the beginning of the second week of infection. PEDV IgG antibodies were detected for at least 16 weeks post-exposure. Remarkably, the serum IgA levels remained high and relatively stable throughout the study, lasting longer than the serum IgG response. Overall, AlphaLISA allows the detection and characterization of pathogen-specific antibodies with new speed, sensitivity, and simplicity of use. Particularly, the bridge assay constitutes a rapid diagnostic that substantially improves upon the “time to result” metric of currently available immunoassays.
    Keywords porcine epidemic diarrhea virus ; humoral immune response ; serum igg ; serum iga ; alphalisa ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  3. Article ; Online: Agrodiag PorCoV: A multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses.

    Malbec, Rémi / Kimpston-Burkgren, Kay / Vandenkoornhuyse, Elisa / Olivier, Christophe / Souplet, Vianney / Audebert, Christophe / Carrillo-Ávila, Jose Antonio / Baum, David / Giménez-Lirola, Luis

    Journal of immunological methods

    2020  Volume 483, Page(s) 112808

    Abstract: Three different porcine enteric coronaviruses (PECs), i.e., porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine Deltacoronavirus (PDCoV) are currently circulating in U.S. commercial swine herds. Differential ... ...

    Abstract Three different porcine enteric coronaviruses (PECs), i.e., porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine Deltacoronavirus (PDCoV) are currently circulating in U.S. commercial swine herds. Differential diagnosis of PECs relies on laboratory methods. This study describes the development of an ELISA-like multiplex planar immunoassay based on virus-specific recombinant S1 proteins printed in an array of spots at the bottom of a 96-well microplate for simultaneous detection differential serodiagnosis of PEDV, TGEV, PDCoV in a single sample. The technology overall format and working principle is similar to the solid-phase standard ELISA. After the three typical incubation steps, the reaction was visualized as blue spots which intensity correlated with antibody levels to specific viral antigen target in the array. The diagnostic performance of the assay was evaluated on known status serum samples (n = 480) collected over time (day post-inoculation -7, 0, 7, 14, 21, 28, 35, and 42) from pigs inoculated with PEDV, TGEV Purdue, TGEV Miller, PDCoV (USA/IL/2014), or mock inoculated with culture media under experimental conditions. Antigen-specific cut-offs were selected to ensure 100% diagnostic and analytical specificity for each given antigen target. The overall diagnostic sensitivity was 92% (44/48 positives, 95% confidence interval (CI) 98,100) for PEDV S1, 100% (95/95 positives, 95% CI 98, 100) for TGEV S1, and 98% (47/48 positives, 95% CI 97, 100) for PDCoV S1. The results of this study demonstrate that the AgroDiag PEC multiplex immunoassay is an efficient and reliable test for differential detection and serodiagnosis of PEDV, TGEV and PDCoV.
    MeSH term(s) Alphacoronavirus/immunology ; Animals ; Antibodies, Viral/blood ; Biomarkers/blood ; Coronavirus Infections/diagnosis ; Coronavirus Infections/immunology ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Deltacoronavirus/immunology ; Diagnosis, Differential ; Enzyme-Linked Immunosorbent Assay/veterinary ; Gastroenteritis, Transmissible, of Swine/diagnosis ; Gastroenteritis, Transmissible, of Swine/immunology ; Gastroenteritis, Transmissible, of Swine/virology ; Immunoglobulin G/blood ; Porcine epidemic diarrhea virus/immunology ; Predictive Value of Tests ; Reproducibility of Results ; Serologic Tests/veterinary ; Swine ; Transmissible gastroenteritis virus/immunology
    Chemical Substances Antibodies, Viral ; Biomarkers ; Immunoglobulin G
    Keywords covid19
    Language English
    Publishing date 2020-06-17
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2020.112808
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 795: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Agrodiag PorCoV: A multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses

    Malbec, Rémi / Kimpston-Burkgren, Kay / Vandenkoornhuyse, Elisa / Olivier, Christophe / Souplet, Vianney / Audebert, Christophe / Carrillo-Ávila, Jose Antonio / Baum, David / Giménez-Lirola, Luis

    Journal of immunological methods. 2020 Aug., v. 483

    2020  

    Abstract: Three different porcine enteric coronaviruses (PECs), i.e., porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine Deltacoronavirus (PDCoV) are currently circulating in U.S. commercial swine herds. Differential ... ...

    Abstract Three different porcine enteric coronaviruses (PECs), i.e., porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine Deltacoronavirus (PDCoV) are currently circulating in U.S. commercial swine herds. Differential diagnosis of PECs relies on laboratory methods. This study describes the development of an ELISA-like multiplex planar immunoassay based on virus-specific recombinant S1 proteins printed in an array of spots at the bottom of a 96-well microplate for simultaneous detection differential serodiagnosis of PEDV, TGEV, PDCoV in a single sample. The technology overall format and working principle is similar to the solid-phase standard ELISA. After the three typical incubation steps, the reaction was visualized as blue spots which intensity correlated with antibody levels to specific viral antigen target in the array. The diagnostic performance of the assay was evaluated on known status serum samples (n = 480) collected over time (day post-inoculation -7, 0, 7, 14, 21, 28, 35, and 42) from pigs inoculated with PEDV, TGEV Purdue, TGEV Miller, PDCoV (USA/IL/2014), or mock inoculated with culture media under experimental conditions. Antigen-specific cut-offs were selected to ensure 100% diagnostic and analytical specificity for each given antigen target. The overall diagnostic sensitivity was 92% (44/48 positives, 95% confidence interval (CI) 98,100) for PEDV S1, 100% (95/95 positives, 95% CI 98, 100) for TGEV S1, and 98% (47/48 positives, 95% CI 97, 100) for PDCoV S1. The results of this study demonstrate that the AgroDiag PEC multiplex immunoassay is an efficient and reliable test for differential detection and serodiagnosis of PEDV, TGEV and PDCoV.
    Keywords Porcine epidemic diarrhea virus ; Transmissible gastroenteritis virus ; analytical specificity ; animal viruses ; antibodies ; blood serum ; confidence interval ; culture media ; diagnostic sensitivity ; enzyme-linked immunosorbent assay ; herds ; proteins ; serodiagnosis ; swine ; viral antigens ; United States
    Language English
    Dates of publication 2020-08
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2020.112808
    Database NAL-Catalogue (AGRICOLA)

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 795: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Agrodiag PorCoV: A multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses

    Malbec, Rémi / Kimpston-Burkgren, Kay / Vandenkoornhuyse, Elisa / Olivier, Christophe / Souplet, Vianney / Audebert, Christophe / Carrillo-Ávila, Jose Antonio / Baum, David / Giménez-Lirola, Luis

    J Immunol Methods

    Abstract: Three different porcine enteric coronaviruses (PECs), i.e., porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine Deltacoronavirus (PDCoV) are currently circulating in U.S. commercial swine herds. Differential ... ...

    Abstract Three different porcine enteric coronaviruses (PECs), i.e., porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine Deltacoronavirus (PDCoV) are currently circulating in U.S. commercial swine herds. Differential diagnosis of PECs relies on laboratory methods. This study describes the development of an ELISA-like multiplex planar immunoassay based on virus-specific recombinant S1 proteins printed in an array of spots at the bottom of a 96-well microplate for simultaneous detection differential serodiagnosis of PEDV, TGEV, PDCoV in a single sample. The technology overall format and working principle is similar to the solid-phase standard ELISA. After the three typical incubation steps, the reaction was visualized as blue spots which intensity correlated with antibody levels to specific viral antigen target in the array. The diagnostic performance of the assay was evaluated on known status serum samples (n = 480) collected over time (day post-inoculation -7, 0, 7, 14, 21, 28, 35, and 42) from pigs inoculated with PEDV, TGEV Purdue, TGEV Miller, PDCoV (USA/IL/2014), or mock inoculated with culture media under experimental conditions. Antigen-specific cut-offs were selected to ensure 100% diagnostic and analytical specificity for each given antigen target. The overall diagnostic sensitivity was 92% (44/48 positives, 95% confidence interval (CI) 98,100) for PEDV S1, 100% (95/95 positives, 95% CI 98, 100) for TGEV S1, and 98% (47/48 positives, 95% CI 97, 100) for PDCoV S1. The results of this study demonstrate that the AgroDiag PEC multiplex immunoassay is an efficient and reliable test for differential detection and serodiagnosis of PEDV, TGEV and PDCoV.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #602160
    Database COVID19

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    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform

    Kay, Kimpston-Burkgren / Mora-Díaz, Juan Carlos / Roby, Philippe / Bjustrom-Kraft, Jordan / Main, Rodger / Bosse, Roger / Giménez-Lirola, Luis Gabriel

    Pathogens

    Abstract: Coronavirus infections are a continuous threat raised time and again With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health Porcine epidemic diarrhea (PED) is considered to be a reemerging ... ...

    Abstract Coronavirus infections are a continuous threat raised time and again With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health Porcine epidemic diarrhea (PED) is considered to be a reemerging pig disease caused by the enteropathogenic alphacoronavirus PED virus (PEDV) In the absence of effective vaccines, infection prevention and control through diagnostic testing and quarantine are critical Early detection and differential diagnosis of PEDV infections increase the chance of successful control of the disease Therefore, there is a continuous need for development of reduced assay-step protocols, no-wash, high-throughput immunoassays This study described the characterization of the humoral immune response against PEDV under experimental and field conditions using a rapid, sensitive, luminescent proximity homogenous assay (AlphaLISA) PEDV IgG and IgA antibodies were developed toward the beginning of the second week of infection PEDV IgG antibodies were detected for at least 16 weeks post-exposure Remarkably, the serum IgA levels remained high and relatively stable throughout the study, lasting longer than the serum IgG response Overall, AlphaLISA allows the detection and characterization of pathogen-specific antibodies with new speed, sensitivity, and simplicity of use Particularly, the bridge assay constitutes a rapid diagnostic that substantially improves upon the “time to result” metric of currently available immunoassays
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #826966
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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