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  1. Book ; Online: Mensch-Technik-Interaktion in medikalisierten Alltagen

    Wöhlke, Sabine / Wöhlke, Sabine / Palm, Anna

    2018  

    Keywords Society & social sciences ; Technisierung ; Artefakt (Archäologie) ; Diabetes mellitus ; Korsett ; Prothese ; Therapie ; Tiefe Hirnstimulation
    Size 1 Online-Ressource
    Publisher Universitätsverlag Göttingen
    Document type Book ; Online
    Note German ; Open Access
    HBZ-ID HT021029386
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article: Benchmarking and Validation of a Bioinformatics Workflow for Meat Species Identification Using 16S rDNA Metabarcoding.

    Denay, Grégoire / Preckel, Laura / Petersen, Henning / Pietsch, Klaus / Wöhlke, Anne / Brünen-Nieweler, Claudia

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 5

    Abstract: DNA-metabarcoding is becoming more widely used for routine authentication of meat-based food and feed products. Several methods validating species identification methods through amplicon sequencing have already been published. These use a variety of ... ...

    Abstract DNA-metabarcoding is becoming more widely used for routine authentication of meat-based food and feed products. Several methods validating species identification methods through amplicon sequencing have already been published. These use a variety of barcodes and analysis workflows, however, no methodical comparison of available algorithms and parameter optimization are published hitherto for meat-based products' authenticity. Additionally, many published methods use very small subsets of the available reference sequences, thereby limiting the potential of the analysis and leading to over-optimistic performance estimates. We here predict and compare the ability of published barcodes to distinguish taxa in the BLAST NT database. We then use a dataset of 79 reference samples, spanning 32 taxa, to benchmark and optimize a metabarcoding analysis workflow for 16S rDNA Illumina sequencing. Furthermore, we provide recommendations as to the parameter choices, sequencing depth, and thresholds that should be used to analyze meat metabarcoding sequencing experiments. The analysis workflow is publicly available, and includes ready-to-use tools for validation and benchmarking.
    Language English
    Publishing date 2023-02-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12050968
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Benchmarking and Validation of a Bioinformatics Workflow for Meat Species Identification Using 16S rDNA Metabarcoding

    Denay, Grégoire / Preckel, Laura / Petersen, Henning / Pietsch, Klaus / Wöhlke, Anne / Brünen-Nieweler, Claudia

    Foods. 2023 Feb. 24, v. 12, no. 5

    2023  

    Abstract: DNA-metabarcoding is becoming more widely used for routine authentication of meat-based food and feed products. Several methods validating species identification methods through amplicon sequencing have already been published. These use a variety of ... ...

    Abstract DNA-metabarcoding is becoming more widely used for routine authentication of meat-based food and feed products. Several methods validating species identification methods through amplicon sequencing have already been published. These use a variety of barcodes and analysis workflows, however, no methodical comparison of available algorithms and parameter optimization are published hitherto for meat-based products’ authenticity. Additionally, many published methods use very small subsets of the available reference sequences, thereby limiting the potential of the analysis and leading to over-optimistic performance estimates. We here predict and compare the ability of published barcodes to distinguish taxa in the BLAST NT database. We then use a dataset of 79 reference samples, spanning 32 taxa, to benchmark and optimize a metabarcoding analysis workflow for 16S rDNA Illumina sequencing. Furthermore, we provide recommendations as to the parameter choices, sequencing depth, and thresholds that should be used to analyze meat metabarcoding sequencing experiments. The analysis workflow is publicly available, and includes ready-to-use tools for validation and benchmarking.
    Keywords DNA barcoding ; barcoding ; bioinformatics ; data collection ; databases ; meat ; species identification
    Language English
    Dates of publication 2023-0224
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12050968
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Epidemiology and zoonotic transmission of

    Nordhoff, Katja / Scharlach, Martina / Effelsberg, Natalie / Knorr, Carolin / Rocker, Dagmar / Claussen, Katja / Egelkamp, Richard / Mellmann, Alexander C / Moss, Andreas / Müller, Ilona / Roth, Sarah Andrea / Werckenthin, Christiane / Wöhlke, Anne / Ehlers, Joachim / Köck, Robin

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1183984

    Abstract: Introduction: The emergence of carbapenem-resistant bacteria causing serious infections may lead to more frequent use of previously abandoned antibiotics like colistin. However, mobile colistin resistance genes (: Methods: In 2018 and 2019, 175 ... ...

    Abstract Introduction: The emergence of carbapenem-resistant bacteria causing serious infections may lead to more frequent use of previously abandoned antibiotics like colistin. However, mobile colistin resistance genes (
    Methods: In 2018 and 2019, 175 environmental (boot swabs of turkey faeces) and 46 human stool samples were analysed using a combination of enrichment-based culture, PCR, core genome multilocus sequence typing (cgMLST) and plasmid typing.
    Results: mcr
    Discussion: These findings confirm that
    Language English
    Publishing date 2023-06-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1183984
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Neurotropic Highly Pathogenic Avian Influenza A(H5N1) Virus in Red Foxes, Northern Germany.

    Baechlein, Christine / Kleinschmidt, Sven / Hartmann, Dorothee / Kammeyer, Patricia / Wöhlke, Anne / Warmann, Tobias / Herms, Louise / Kühl, Bianca / Beineke, Andreas / Wohlsein, Peter / Harder, Timm / Runge, Martin

    Emerging infectious diseases

    2023  Volume 29, Issue 12, Page(s) 2509–2512

    Abstract: In a 1-year survey of wild terrestrial predators in northern Germany, we found that 5 of 110 foxes were infected with contemporary avian influenza A(H5N1) viruses, forming a temporal cluster during January‒March 2023. Encephalitis and strong cerebral ... ...

    Abstract In a 1-year survey of wild terrestrial predators in northern Germany, we found that 5 of 110 foxes were infected with contemporary avian influenza A(H5N1) viruses, forming a temporal cluster during January‒March 2023. Encephalitis and strong cerebral virus replication but only sporadic mammalian-adaptive viral polymerase basic 2 protein E627K mutations were seen.
    MeSH term(s) Animals ; Humans ; Influenza in Birds/epidemiology ; Influenza A Virus, H5N1 Subtype/genetics ; Foxes ; Viral Proteins/genetics ; Germany/epidemiology
    Chemical Substances Viral Proteins
    Language English
    Publishing date 2023-11-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2912.230938
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4778: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Medizinanthropologische Annäherungen an medikalisierte Beziehungen im Alltag. Einleitung in den Themenschwerpunkt Verwandtschaft, Sorge, Technologien

    Palm, Anna / Wöhlke, Sabine

    Curare

    2019  Volume 42, Issue 1/2, Page(s) 9

    Language German ; English
    Document type Article
    ZDB-ID 303901-8
    ISSN 0344-8622
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2130: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  7. Article: Verwandtschaft, Sorge, Technologien. Medikalisierte Beziehungen im Alltag. Herausgegeben von Anna Palm & Sabine Wöhlke

    Palm, Anna / Wöhlke, Sabine

    Curare

    2019  Volume 42, Issue 1/2, Page(s) 5

    Language German ; English
    Document type Article
    ZDB-ID 303901-8
    ISSN 0344-8622
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2130: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Impact of wet-lab protocols on quality of whole-genome short-read sequences from foodborne microbial pathogens.

    Forth, Leonie F / Brinks, Erik / Denay, Grégoire / Fawzy, Ahmad / Fiedler, Stefan / Fuchs, Jannika / Geuthner, Anne-Catrin / Hankeln, Thomas / Hiller, Ekkehard / Murr, Larissa / Petersen, Henning / Reiting, Ralf / Schäfers, Christian / Schwab, Claudia / Szabo, Kathrin / Thürmer, Andrea / Wöhlke, Anne / Fischer, Jennie / Lüth, Stefanie /
    Projahn, Michaela / Stingl, Kerstin / Borowiak, Maria / Deneke, Carlus / Malorny, Burkhard / Uelze, Laura

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1253362

    Abstract: For successful elucidation of a food-borne infection chain, the availability of high-quality sequencing data from suspected microbial contaminants is a prerequisite. Commonly, those investigations are a joint effort undertaken by different laboratories ... ...

    Abstract For successful elucidation of a food-borne infection chain, the availability of high-quality sequencing data from suspected microbial contaminants is a prerequisite. Commonly, those investigations are a joint effort undertaken by different laboratories and institutes. To analyze the extent of variability introduced by differing wet-lab procedures on the quality of the sequence data we conducted an interlaboratory study, involving four bacterial pathogens, which account for the majority of food-related bacterial infections:
    Language English
    Publishing date 2023-11-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1253362
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Neurotropic highly pathogenic avian influenza A(H5N1) virus in red foxes, northern Germany

    Baechlein, Christine / Kleinschmidt, Sven / Hartmann, Dorothee / Kammeyer, Patricia / Wöhlke, Anne / Warmann, Tobias / Herms, Louise / Kühl, Bianca / Beineke, Andreas / Wohlsein, Peter / Harder, Timm C. / Runge, Martin

    2023  

    Abstract: In a 1-year survey of wild terrestrial predators in northern Germany, we found that 5 of 110 foxes were infected with contemporary avian influenza A(H5N1) viruses, forming a temporal cluster during January‒March 2023. Encephalitis and strong cerebral ... ...

    Abstract In a 1-year survey of wild terrestrial predators in northern Germany, we found that 5 of 110 foxes were infected with contemporary avian influenza A(H5N1) viruses, forming a temporal cluster during January‒March 2023. Encephalitis and strong cerebral virus replication but only sporadic mammalian-adaptive viral polymerase basic 2 protein E627K mutations were seen.
    Keywords Text ; article ; ddc:630
    Language English
    Publishing date 2023-10-31
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Book ; Thesis: Fine mapping of the genomic region of the bovine polled locus

    Wöhlke, Anne

    2004  

    Author's details vorgelegt von Anne Wöhlke
    Language English
    Size 119 S, graph. Darst
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Tierärztl. Hochsch., Inst. für Tierzucht und Vererbungsforschung, Diss.--Hannover, 2004
    Note Auch als elektronisches Dokument vorh.
    Database Special collection on veterinary medicine and general parasitology

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