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Article ; Online: Structure and energetics guide dynamic behaviour in a T = 3 icosahedral virus capsid.

Shrivastav, Gourav / Borkotoky, Subhomoi / Dey, Debajit / Singh, Bhumika / Malhotra, Nidhi / Azad, Kimi / Jayaram, B / Agarwal, Manish / Banerjee, Manidipa

Biophysical chemistry

2023 Volume 305, Page(s) 107152

Abstract: Although virus capsids appear as rigid, symmetric particles in experimentally determined structures; biochemical studies suggest a significant degree of structural flexibility in the particles. We carried out all-atom simulations on the icosahedral ... ...

Abstract	Although virus capsids appear as rigid, symmetric particles in experimentally determined structures; biochemical studies suggest a significant degree of structural flexibility in the particles. We carried out all-atom simulations on the icosahedral capsid of an insect virus, Flock House Virus, which show intriguing differences in the degree of flexibility of quasi-equivalent capsid subunits consistent with previously described biological behaviour. The flexibility of all the β and γ subunits of the protein and RNA fragments is analysed and compared. Both γ
MeSH term(s)	Capsid/chemistry ; Capsid/metabolism ; Interleukin Receptor Common gamma Subunit/analysis ; Interleukin Receptor Common gamma Subunit/metabolism ; Capsid Proteins/analysis ; Capsid Proteins/metabolism ; RNA/metabolism ; Water/metabolism
Chemical Substances	Interleukin Receptor Common gamma Subunit ; Capsid Proteins ; RNA (63231-63-0) ; Water (059QF0KO0R)
Language	English
Publishing date	2023-12-08
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	185052-0
ISSN	1873-4200 ; 0301-4622
ISSN (online)	1873-4200
ISSN	0301-4622
DOI	10.1016/j.bpc.2023.107152
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Article ; Online: Papillary adenoma in the trachea of a wild boar (Sus scrofa)

Madhu Swamy / Nidhi Rajput / A. B. Shrivastav

Veterinary World, Vol 6, Iss 3.000, Pp 169-

2013 Volume 170

Abstract: A rare report of papillary adenoma in the trachea of a free-ranging wild boar has been reported in the article. In the case, except for the growth in the trachea, there were no other significant lesions observed in the carcass. Grossly, the adenoma was ... ...

Abstract	A rare report of papillary adenoma in the trachea of a free-ranging wild boar has been reported in the article. In the case, except for the growth in the trachea, there were no other significant lesions observed in the carcass. Grossly, the adenoma was granular and microscopically, it comprised finger like projections with the features of hyperplastic glandular epithelium and increased mitotic figures. This case is first of its kind and also its occurrence is negligible in other animals. Few cases have been reported in the humans with the history of excessive smoking. Although, adenomas of other organs are common in pigs. However, authors are unaware of previous reports of such case in the animals. [Vet World 2013; 6(3.000): 169-170]
Keywords	papillary adenoma ; trachea ; wild boar ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2013-06-01T00:00:00Z
Publisher	Veterinary World
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Direct impact of gonadotropins on glucose uptake and storage in preovulatory granulosa cells: Implications in the pathogenesis of polycystic ovary syndrome.

Chahal, Nidhi / Geethadevi, Anjali / Kaur, Surleen / Lakra, Ruchi / Nagendra, Anjali / Shrivastav, T G / De Pascali, Francesco / Reiter, Eric / Crépieux, Pascale / Devi, M Gouri / Malhotra, Neena / Muralidhar, K / Singh, Rita

Metabolism: clinical and experimental

2020 Volume 115, Page(s) 154458

Abstract: Background: Polycystic ovary syndrome (PCOS) is often associated with higher levels of LH, and arrested ovarian follicular growth. The direct impact of high LH on FSH mediated metabolic responses in PCOS patients is not clearly understood.: Method: ... ...

Abstract	Background: Polycystic ovary syndrome (PCOS) is often associated with higher levels of LH, and arrested ovarian follicular growth. The direct impact of high LH on FSH mediated metabolic responses in PCOS patients is not clearly understood. Method: In order to investigate the impact of FSH and LH on glucose metabolism in preovulatory granulosa cells (GCs), we used [U Results: In normal human and rat granulosa cells, FSH is more potent than hCG in stimulating glucose uptake, however glycogen synthesis was significantly upregulated only by FSH through increase in activity of glycogen synthase via IRS-2/PI3K/Akt2 pathway. On the contrary, an impaired FSH-stimulated glucose uptake and glycogen synthesis in granulosa cells of PCOS-patients indicated a selective defect in FSHR activation. Further, in normal human granulosa cells, and in immature rat model, the impact of hCG on FSH responses was such that it inhibited the FSH-mediated glucose uptake as well as glycogen synthesis through inhibition of FSH-stimulated IRS-2 expression. These findings were further validated in HEK293 cells overexpressing Flag-LHR and HA-FSHR, where high hCG inhibited the FSH-stimulated glucose uptake. Notably, an increased BRET change was observed in HEK293 cells expressing FSHR-Rluc8 and LHR-Venus possibly suggesting increased heteromerization of LHR and FSHR in the presence of both hCG and FSH in comparison to FSH or hCG alone. Conclusion: Our findings confirm a selective attenuation of metabolic responses to FSH such as glucose uptake and glycogen synthesis by high activation level of LHR leading to the inhibition of IRS-2 pathway, resulting in depleted glycogen stores and follicular growth arrest in PCOS patients.
MeSH term(s)	Animals ; Disease Models, Animal ; Estradiol/pharmacology ; Female ; Follicle Stimulating Hormone/pharmacology ; Glucose/metabolism ; Granulosa Cells/drug effects ; Granulosa Cells/metabolism ; HEK293 Cells ; Humans ; Insulin Receptor Substrate Proteins/metabolism ; Luteinizing Hormone/pharmacology ; Polycystic Ovary Syndrome/metabolism ; Rats
Chemical Substances	IRS2 protein, human ; Insulin Receptor Substrate Proteins ; Estradiol (4TI98Z838E) ; Luteinizing Hormone (9002-67-9) ; Follicle Stimulating Hormone (9002-68-0) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2020-12-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80230-x
ISSN	1532-8600 ; 0026-0495
ISSN (online)	1532-8600
ISSN	0026-0495
DOI	10.1016/j.metabol.2020.154458
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Article ; Online: Development of molecular tools to differentiate Indian wild pig (Sus scrofa cristatus) meat from exotic and local domestic pig meat

Kajal Kumar Jadav / Avadh Bihari Shrivastav / Nidhi Rajput

Veterinary World, Vol 6, Iss 11, Pp 919-

2013 Volume 922

Abstract: Aim: Identification of wild pig and domestic pig is essential to prevent illegal poaching of wild pig and to implement Wildlife (Protection) Act, 1972. PCR-RFLP was used to differentiate Wild pig (Sus scrofa cristatus) from Domestic pig (Sus scrofa ... ...

Abstract	Aim: Identification of wild pig and domestic pig is essential to prevent illegal poaching of wild pig and to implement Wildlife (Protection) Act, 1972. PCR-RFLP was used to differentiate Wild pig (Sus scrofa cristatus) from Domestic pig (Sus scrofa domestica) meat. Materials and Methods: DNA was isolated from meat samples of both the sub species and a fragment of Cytochrome b gene was amplified using universal primers and the PCR products were subjected to restriction digestion. Results: All the known samples of each of the sub-species amplified 474 bp fragment successfully using b1 and b2 primers. To differentiate between wild and domestic pig meat, restriction digestion of the PCR products was carried out to produce characteristic PCR-RFLP patterns for each species. StuI digestion yielded a RFLP pattern which distinguished the closely related sub species. The alignment of sequences of Wild pigs with sequences of local domestic pig, European wild pig and exotic breeds revealed 7 intra-species polymorphic sites within Cytochrome b gene fragment.Conclusion: This study showed that The PCR-RFLP is a simple and very effective tool for differentiating the samples of both the sub species and could prove to be a useful tool in forensic identification of wild pig and domestic pig.
Keywords	cytochrome b gene ; domestic pig ; Indian wild pig ; PCR-RFLP ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2013-10-01T00:00:00Z
Publisher	Veterinary World
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Single nucleotide markers of D-loop for identification of Indian wild pig (Sus scrofa cristatus).

Srivastava, Gaurav Kumar / Rajput, Nidhi / Jadav, Kajal Kumar / Shrivastav, Avadh Bihari / Joshi, Himanshu R

Veterinary world

2015 Volume 8, Issue 4, Page(s) 532–536

Abstract: Aim: Partial fragment of D-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (SNP) markers in the ... ...

Abstract	Aim: Partial fragment of D-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (SNP) markers in the region. The paper also reappraises SNP markers from two fragments of cytochrome b gene and a fragment 12S rRNA gene distinguishing the Indian wild pig from other pig species of the world. Materials and methods: Deoxyribonucleic acid (DNA) was isolated from 14 and 12 tissue samples of Indian wild and domestic pigs, respectively, collected from Central India for characterization of the D-loop DNA sequences using universal primers. The sequences obtained were aligned along with the retrieved sequences to analyze species-specific SNP marker. Results: A total of 58 mitochondrial D-loop gene sequences of pig races were aligned to identify 1349 polymorphic sites in the fragment from nucleotide positions 35-770 bp and four SNP markers were identified to differentiate Indian wild pig from all the sequences investigated in this study. With the inclusion of cytochrome b gene and 12S rRNA gene fragments, the present study contributes to the total 15 SNP markers, which have been identified in the mitochondrial fragment of 1936 bp for identification of Indian wild pig. Conclusion: SNP markers have advantages over other marker types and do not require subsequent standardization to compare data across studies or laboratories.
Language	English
Publishing date	2015-04-24
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2015.532-536
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Article: Investigations on prevalence of theileriosis in cattle by polymerase chain reaction

Agrawal, Vivek / Das, G / Shrivastav, A.B / Singh, Nidhi / Singh, A.P

Journal of veterinary parasitology. 2016 June, v. 30, no. 1

2016

Abstract: Apolymerase chain reaction (PCR) based assay was standardized forthe specific and sensitive detection of Theileria annulata infection in blood samples collected from cattle in and around Jabalpur, Madhya Pradesh, India. Examination of stained blood ... ...

Abstract	Apolymerase chain reaction (PCR) based assay was standardized forthe specific and sensitive detection of Theileria annulata infection in blood samples collected from cattle in and around Jabalpur, Madhya Pradesh, India. Examination of stained blood smears revealed a low prevalence rate (7.2%) of T. annulata while 20.28% samples were found to be positive by PCR. Our study based on the PCR protocol revealed that the prevalence of T. annulata was more in adult female bovines during monsoon season.
Keywords	Theileria annulata ; adults ; blood ; cattle ; females ; monsoon season ; polymerase chain reaction ; theileriosis ; veterinary parasitology ; India
Language	English
Dates of publication	2016-06
Size	p. 1-5.
Publishing place	The Indian Association for the Advancement of Veterinary Parasitology
Document type	Article
Note	NAL-AP-2-clean
ISSN	0974-0813
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation.

Shrivastav, Nidhi / Li, Deyu / Essigmann, John M

Carcinogenesis

2009 Volume 31, Issue 1, Page(s) 59–70

Abstract: The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they can disrupt expression of genes. ... ...

Abstract	The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they can disrupt expression of genes. Determination of which adduct is responsible for each of these biological endpoints is difficult, but this task has been accomplished for some carcinogenic DNA-damaging agents. Here, we describe the respective contributions of specific DNA lesions to the biological effects of low molecular weight alkylating agents.
MeSH term(s)	Alkylation ; DNA/drug effects ; DNA Damage ; DNA Repair/drug effects ; Mutagenesis
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2009-10-29
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	603134-1
ISSN	1460-2180 ; 0143-3334
ISSN (online)	1460-2180
ISSN	0143-3334
DOI	10.1093/carcin/bgp262
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Article ; Online: Weed dynamics and productivity of spring maize under different tillage and weed management methods

Nidhi Shrivastav / Komal Bahadur Basnet / Lal Prasad Amgain / Tika Bahadur Karki / Narayan Khatri

Azarian Journal of Agriculture, Vol 2, Iss 5, Pp 118-

2015 Volume 122

Abstract: A field experiment was conducted during spring season of 2013 on sandy loam soil at Rampur, Chitwan, Nepal to study the effect of tillage and weed management methods on weeds dynamics and productivity of spring maize (Zea mays L.). Two tillage methods ... ...

Abstract	A field experiment was conducted during spring season of 2013 on sandy loam soil at Rampur, Chitwan, Nepal to study the effect of tillage and weed management methods on weeds dynamics and productivity of spring maize (Zea mays L.). Two tillage methods namely zero and conventional and six weed management methods namely weedy check, weed free, atrazine 1.5 kg/ha as pre emergence, atrazine and glyphosate as pre emergence, atrazine pendimethalin as pre emergence and atrazine and one hand weeding (HW) at 40 Days after sowing were tested in a strip plot design with three replications. Total weed density and dry weight recorded in conventional tillage were significantly higher than that of zero tillage at almost all growth stages. The tank mixed application of atrazine with glyphosate was found significantly more effective than with pendimethalin and Hand Weeding at 40 Days after sowing. The grain yield of maize was not affected significantly by tillage methods but it was lower in conventional tillage in comparison to zero tillage. The grain yields obtained in double combinations of atrazine with glyphosate (6.69 t/ ha), pendimethalin (6.24 t/ha) and HW at 40 DAS (6.48 t/ ha) were comparable to each other but significantly superior over its sole application and equally effective as weed free condition (7.18 t/ ha). Thus, the maize can be successfully cultivated in zero tillage and combination of atrazine either with glyphosate or with HW at 40 DAS as alternatives of manual weeding to achieve higher grain yield in spring season.
Keywords	Grain yield ; Spring maize ; Tillage and Weed management methods ; Agriculture (General) ; S1-972 ; Plant culture ; SB1-1110
Subject code	630 ; 571
Language	English
Publishing date	2015-10-01T00:00:00Z
Publisher	Azarian Journals
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Single nucleotide markers of D-loop for identification of Indian wild pig (Sus scrofa cristatus)

Gaurav Kumar Srivastava / Nidhi Rajput / Kajal Kumar Jadav / Avadh Bihari Shrivastav / Himanshu R. Joshi

Veterinary World, Vol 8, Iss 4, Pp 532-

2015 Volume 536

Abstract	Aim: Partial fragment of D-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (SNP) markers in the region. The paper also reappraises SNP markers from two fragments of cytochrome b gene and a fragment 12S rRNA gene distinguishing the Indian wild pig from other pig species of the world. Materials and Methods: Deoxyribonucleic acid (DNA) was isolated from 14 and 12 tissue samples of Indian wild and domestic pigs, respectively, collected from Central India for characterization of the D-loop DNA sequences using universal primers. The sequences obtained were aligned along with the retrieved sequences to analyze species-specific SNP marker. Results: A total of 58 mitochondrial D-loop gene sequences of pig races were aligned to identify 1349 polymorphic sites in the fragment from nucleotide positions 35-770 bp and four SNP markers were identified to differentiate Indian wild pig from all the sequences investigated in this study. With the inclusion of cytochrome b gene and 12S rRNA gene fragments, the present study contributes to the total 15 SNP markers, which have been identified in the mitochondrial fragment of 1936 bp for identification of Indian wild pig. Conclusion: SNP markers have advantages over other marker types and do not require subsequent standardization to compare data across studies or laboratories.
Keywords	D-loop ; Indian wild pig ; single nucleotide markers ; species identification ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2015-04-01T00:00:00Z
Publisher	Veterinary World
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Polymerase chain reaction based detection of Mycobacterium tuberculosis IS6110 in cattle faeces

Bassessar, Varun / Shrivastav, A.B. / Swamy, Madhu / Jadhav, Kajal / Rajput, Nidhi

Indian journal of veterinary pathology. 2014 Mar. 1, v. 38, no. 1

2014

Abstract: PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis in fecal samples was evaluated in the diagnosis of intestinal tuberculosis (ITB). Single intradermal tuberculin test was carried in 125 cattle from organized dairy farms, off ... ...

Abstract	PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis in fecal samples was evaluated in the diagnosis of intestinal tuberculosis (ITB). Single intradermal tuberculin test was carried in 125 cattle from organized dairy farms, off which, 36 animals gave a positive reaction. Blood and faecal samples were collected from all the tuberculin positive (36) animals. Demonstration of acid fast bacilli was done in the faecal samples by using the Ziehl - Neelsen staining. DNA was extracted and PCR was carried out in the blood and faecal samples by using IS6110 insertion sequence. Acid fast bacilli were observed in 10 (27.77%) faecal smears whereas, 18 (50%) faecal samples and 4 (11.11%) blood samples were found positive for the insertion sequence IS6110 of Mycobacterium tuberculosis complex. This result shows that IS6110 insertion sequence-PCR assay may be applied to detect tuberculous bacilli directly from faecal sample of dairy cows and therefore could be a valuable diagnostic or screening test for herds.
Keywords	DNA ; Mycobacterium tuberculosis ; animal pathology ; blood ; feces ; intestines ; polymerase chain reaction ; transposons ; tuberculin ; tuberculosis
Language	English
Dates of publication	2014-0301
Size	p. 10-13.
Publishing place	Indian Association of Veterinary Pathologists
Document type	Article
ISSN	0973-970X
DOI	10.5958/0973-970X.2014.01126.2
Database	NAL-Catalogue (AGRICOLA)

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