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Article ; Online: Accréditation des cryoglobulines : retour d’expérience du CHU de Lyon.

Dechomet, Magali / Kolopp-Sarda, Marie-Nathalie / Dimet, Isabelle / Lombard, Christine

2021 Volume 79, Issue 2, Page(s) 190–195

Abstract: Research and identification of a serum cryoglobulin is a current laboratory test. This analysis is complex to accredit due to, firstly, very strict pre-analytical requirements, secondly, lack of standardization between laboratories in carrying out the ... ...

Title translation	Accreditation of cryoglobulins: Experience of CHU of Lyon.
Abstract	Research and identification of a serum cryoglobulin is a current laboratory test. This analysis is complex to accredit due to, firstly, very strict pre-analytical requirements, secondly, lack of standardization between laboratories in carrying out the different phases of sampling until results validation. The method validation of this analysis, carried out to meet requirements of the NF EN ISO 15189 standard, is a complex process divided into three sub-processes: cryoglobulin research including detection and isolation of cryoprecipitate, typing by immunofixation and quantification of cryoglobulin by immunoglobulin assays. This work made it possible, thanks to the development of a risk matrix taking into account all pre-analytical, analytical and post-analytical phases, to stress the importance of technical proficiency and management of critical equipment . With this approach, the laboratory also checked homogeneity of results validation practices.
MeSH term(s)	Accreditation ; Cryoglobulins ; Humans ; Laboratories ; Reference Standards ; Research Design
Chemical Substances	Cryoglobulins
Language	French
Publishing date	2021-03-26
Publishing country	France
Document type	Journal Article
ZDB-ID	418098-7
ISSN	1950-6112 ; 0003-3898
ISSN (online)	1950-6112
ISSN	0003-3898
DOI	10.1684/abc.2021.1631
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Article ; Online: Supplementation with vitamin D or ω-3 fatty acids in adolescent girls and young women with endometriosis (SAGE): a double-blind, randomized, placebo-controlled trial.

Nodler, James L / DiVasta, Amy D / Vitonis, Allison F / Karevicius, Sarah / Malsch, Maggie / Sarda, Vishnudas / Fadayomi, Ayotunde / Harris, Holly R / Missmer, Stacey A

The American journal of clinical nutrition

2020 Volume 112, Issue 1, Page(s) 229–236

Abstract: ... D and omega-3 (n-3) fatty acid supplementation on endometriosis-associated pain in adolescents ... Objectives: We aimed to determine whether supplementation with vitamin D or ω-3 fatty acids remediates pain ... 27 to vitamin D3; 20 to fish oil; and 22 to placebo. Participants in the vitamin D arm experienced ...

Abstract	Background: Adolescents with endometriosis are a particularly underserved population who struggle with chronic pain. Despite widespread use, there are no published trials examining the individual effects of vitamin D and omega-3 (n-3) fatty acid supplementation on endometriosis-associated pain in adolescents. Objectives: We aimed to determine whether supplementation with vitamin D or ω-3 fatty acids remediates pain, changes frequency of pain medication usage, or affects quality of life in young women with endometriosis. Methods: Women (aged 12-25 y) with surgically confirmed endometriosis and pelvic pain enrolled in a double-blind, randomized, placebo-controlled trial. The primary outcome was pain measured by the visual analog scale (VAS). Secondary outcomes were quality of life, pain catastrophizing, and pain medication usage. Participants were randomly assigned to receive 2000 IU vitamin D3, 1000 mg fish oil, or placebo daily for 6 mo. Results: A total of 147 women were screened and 69 were randomly assigned as follows: 27 to vitamin D3; 20 to fish oil; and 22 to placebo. Participants in the vitamin D arm experienced significant improvement in VAS pain [mean (95% CI) worst pain in the past month, from baseline to 6 mo: 7.0 (6.2, 7.8) to 5.5 (4.2, 6.8), P = 0.02]; however, an improvement of nearly identical magnitude was observed in the placebo arm [6.0 (5.1, 6.9) to 4.4 (3.0, 5.8), P = 0.07]. A more modest improvement was observed in the fish oil arm [5.9 (4.8, 7.0) to 5.2 (3.7, 6.8), P = 0.39]. Neither of the intervention arms were statistically different from placebo. Conclusions: In young women with endometriosis, supplementation with vitamin D led to significant changes in pelvic pain; however, these were similar in magnitude to placebo. Supplementation with fish oil resulted in about half of the VAS pain reduction of the other 2 arms. Studies are needed to better define the physiology underlying the observed reduction in pain score in the placebo arm that persisted across 6 mo.This trial was registered at clinicaltrials.gov as NCT02387931.
MeSH term(s)	Adolescent ; Adult ; Cholecalciferol/administration & dosage ; Dietary Supplements ; Double-Blind Method ; Endometriosis/drug therapy ; Endometriosis/psychology ; Fatty Acids, Omega-3/administration & dosage ; Female ; Humans ; Pelvic Pain/drug therapy ; Placebo Effect ; Quality of Life ; Young Adult
Chemical Substances	Fatty Acids, Omega-3 ; Cholecalciferol (1C6V77QF41)
Language	English
Publishing date	2020-05-20
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	280048-2
ISSN	1938-3207 ; 0002-9165
ISSN (online)	1938-3207
ISSN	0002-9165
DOI	10.1093/ajcn/nqaa096
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Article ; Online: Implant de dexaméthasone (Ozurdex) dans l’œdème maculaire diabétique : efficacité, et sécurité d’utilisation en vraie vie.

Majstruk, L / Qu-Knafo, L / Sarda, V / Fajnkuchen, F / Nghiem-Buffet, S / Grenet, T / Chaine, G / Giocanti-Auregan, A

Journal francais d'ophtalmologie

2020 Volume 43, Issue 3, Page(s) 197–204

Abstract: Purpose: The goal of this study was to investigate the safety and efficacy of the intravitreal dexamethasone implant (DI) for patients with diabetic macular edema (DME) in real life.: Methods: We conducted a monocentric retrospective analysis of the ... ...

Title translation	Dexamethasone intravitreal implant (Ozurdex) in patients with diabetic macular edema: Real life safety and efficacy.
Abstract	Purpose: The goal of this study was to investigate the safety and efficacy of the intravitreal dexamethasone implant (DI) for patients with diabetic macular edema (DME) in real life. Methods: We conducted a monocentric retrospective analysis of the change in visual acuity and central macular thickness (CMT) after intravitreal injection of the DI at peak efficacy (2 months after injection) as well as the timing of reinjections and complications in patients with a loss of vision due to DME. Results: Forty eyes of 33 patients were included, with a mean follow-up of 12.6 months. Thirty percent of the eyes experienced an increase in best corrected visual acuity (BCVA)>15 letters at peak efficacy (P<0.05) after each ID injection. Treatment-naive patients had a sustained response after Ozurdex injection, with better visual acuity at 3 months (P=0.02) and 4 months (P=0.04) than non-naïve patients and better visual acuity at 6 months despite identical baseline visual acuity (P<0.05). Anatomical efficacy was good, with approximately 60% of patients with CMT<300 microns at peak efficacy after each injection of ID. Thirty percent of eyes demonstrated ocular hypertension (OHT)>25mmHg at peak efficacy, and 12.5% of eyes required cataract surgery during follow-up. Conclusion: The DI has good functional and anatomic efficacy in these patients, with a good safety profile. Treatment-naïve patients with more recent DME had a more sustained increase in visual acuity after the injections and better visual recovery at 6 months. This encourages us to initiate DI therapy early if there is no response to anti-vascular endothelial growth factor (anti-VEGF) treatment.
MeSH term(s)	Aged ; Dexamethasone/administration & dosage ; Dexamethasone/adverse effects ; Diabetes Mellitus, Type 2/complications ; Diabetes Mellitus, Type 2/drug therapy ; Diabetes Mellitus, Type 2/epidemiology ; Diabetes Mellitus, Type 2/pathology ; Diabetic Retinopathy/drug therapy ; Diabetic Retinopathy/epidemiology ; Drug Implants ; Female ; Fovea Centralis/diagnostic imaging ; Fovea Centralis/drug effects ; Fovea Centralis/pathology ; France/epidemiology ; Humans ; Intravitreal Injections ; Macular Edema/drug therapy ; Macular Edema/epidemiology ; Male ; Middle Aged ; Retrospective Studies ; Risk Factors ; Treatment Outcome ; Visual Acuity/drug effects
Chemical Substances	Drug Implants ; Dexamethasone (7S5I7G3JQL)
Language	French
Publishing date	2020-01-27
Publishing country	France
Document type	Journal Article
ZDB-ID	426662-6
ISSN	1773-0597 ; 0181-5512
ISSN (online)	1773-0597
ISSN	0181-5512
DOI	10.1016/j.jfo.2019.08.010
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Article: Novel Use of VP Shunt Stylet for Difficult Catheterisation in Proximal Hypospadias.

Sarda, Dinesh

Journal of Indian Association of Pediatric Surgeons

2023 Volume 28, Issue 2, Page(s) 180–181

Language	English
Publishing date	2023-03-03
Publishing country	India
Document type	Journal Article
ZDB-ID	2164528-0
ISSN	1998-3891 ; 0971-9261
ISSN (online)	1998-3891
ISSN	0971-9261
DOI	10.4103/jiaps.jiaps_177_22
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Article: Evaluation of Metabolic Syndrome and Vitamin D Receptor Gene Polymorphism in Male Factor Infertility.

Bhakat, R / Chandra, L / Saxena, A / Sarda, A K / Krishnamurthy, K / Yadav, P

Indian journal of clinical biochemistry : IJCB

2017 Volume 32, Issue 4, Page(s) 468–472

Abstract: Male factor infertility (MFI) is a growing health issue in society. The vitamin D receptor gene ...

Abstract	Male factor infertility (MFI) is a growing health issue in society. The vitamin D receptor gene (VDR) is expressed in many tissues and modulates expression of several other genes. The purpose of this study was to investigate the association between MetSyn, VDR gene (
Language	English
Publishing date	2017-01-19
Publishing country	India
Document type	Journal Article
ZDB-ID	1033583-3
ISSN	0974-0422 ; 0970-1915
ISSN (online)	0974-0422
ISSN	0970-1915
DOI	10.1007/s12291-017-0632-y
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Article: Human Dirofilariasis Due to D.Repens Presenting as a Chest Wall Swelling : A Rare Case Report

Permi, Harish S. / D'Souza, Pretty / Bhagavan, K.R. / Raju, Mary / Sarda, Pooja

Journal of Health and Allied Sciences NU

2012 Volume 02, Issue 01, Page(s) 51–53

Abstract: ... bone, cartilage, or fat. We report a case of Human Dirofilariasis due to D. Repens occurring ...

Abstract	Primary Dirofilariasis is caused by a Zoonotic filarial nematode. It is transmitted to humans by Culex, Aedes, or Anopheles mosquitoes, which ingest blood-containing microfilaria from affected dogs, cats, or raccoons. Chest wall tumors are uncommon lesions that originate from blood vessels, nerves, bone, cartilage, or fat. We report a case of Human Dirofilariasis due to D. Repens occurring in the chest wall in a 32 year old male. Clinical diagnosis of benign chest wall tumor was considered and it was excised. Histopathological examination confirmed it as Dirofilaria repens. On regular follow up he is doing fine.
Keywords	Dirofilariasis ; D. Repens ; Chest wall swelling
Language	English
Publishing date	2012-03-01
Publisher	Thieme Medical and Scientific Publishers Private Ltd.
Publishing place	Stuttgart ; New York
Document type	Article
ISSN	2582-4953 ; 2582-4287
ISSN (online)	2582-4953
ISSN	2582-4287
DOI	10.1055/s-0040-1703559
Database	Thieme publisher's database

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Article: Predisposing and Overall Effects of Reproductive Hormones on Breast Cancer: A Review.

Sarda, Aditya K / Jogdand, Sangita D

Cureus

2023 Volume 15, Issue 9, Page(s) e45956

Abstract: Cancer, the second leading cause of mortality worldwide, has been the subject of extensive and quickly changing scientific study and practice. Cancer remains a mystery despite the enormous effort put into understanding the genesis of cancerous cells, the ...

Abstract	Cancer, the second leading cause of mortality worldwide, has been the subject of extensive and quickly changing scientific study and practice. Cancer remains a mystery despite the enormous effort put into understanding the genesis of cancerous cells, the development of malignant tissues, and the process by which they propagate and recur. Cells from humans that have been recruited by cancer and, to some extent, changed into pathogenic organisms or the foundation of tumors serve as agents of destruction. Understanding cancers leads to challenging philosophical issues since they undermine and use multicellular organization processes. Cancer metastasizing cells adopt new phenotypes while discarding previous behaviors. The absence of comprehensive knowledge of this has hampered the development of therapeutics for metastatic illness. For systems-level experimental and computational metastasis modeling, integrating these complex and interconnected features continues to be a problem because metastasis has typically been studied in separate physiological compartments. Lung, breast, and prostate cancers accounted for the bulk of the 18 million new cases of cancer that were diagnosed in 2018. The most frequent cancer in women is breast cancer. Animal experimentation plays a significant role in primary and translational breast cancer research. In theory, such breast cancer models should be comparable to breast cancer in humans in terms of tumor etiology, biological behavior, pathology, and treatment response.
Language	English
Publishing date	2023-09-25
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.45956
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Article ; Online: Almond (Prunus dulcis (Mill.) D.A. Webb) polyphenols: from chemical characterization to targeted analysis of phenolic metabolites in humans.

Bartolomé, Begoña / Monagas, María / Garrido, Ignacio / Gómez-Cordovés, Carmen / Martín-Alvarez, Pedro J / Lebrón-Aguilar, Rosa / Urpí-Sardà, Mireia / Llorach, Rafael / Andrés-Lacueva, Cristina

Archives of biochemistry and biophysics

2010 Volume 501, Issue 1, Page(s) 124–133

Abstract: In this paper, a survey of our studies on almond polyphenols including their chemical characterization and further bioavailability in humans is reported. Combination of analytical techniques (LC-DAD/fluorescence, LC/ESI-MS and MALDI-TOF-MS) allowed us, ... ...

Abstract	In this paper, a survey of our studies on almond polyphenols including their chemical characterization and further bioavailability in humans is reported. Combination of analytical techniques (LC-DAD/fluorescence, LC/ESI-MS and MALDI-TOF-MS) allowed us, for the first time, the identification of A- and B-type procyanidin, propelargonidin and prodelphinidin polymers in almond skins. Glucuronide, O-methyl glucuronide, sulfate and O-methyl sulfate derivatives of (epi)catechin, as well as the glucuronide conjugates of naringenin and isorhamnetin, and sulfate conjugates of isorhamnetin, together with conjugates of hydroxyphenylvalerolactones were detected in plasma and urine samples after the intake of almond skin polyphenols. In addition, numerous microbial-derived metabolites, including hydroxyphenylpropionic, hydroxyphenylacetic, hydroxycinnamic, hydroxybenzoic and hydroxyhippuric acids were also identified. Depending of the type of metabolite, maximum urinary excretion was attained at different time in comparison to the control group in the course of the 24-h period of urine excretion, allowing us to establish the onset of microbial metabolism.
MeSH term(s)	Adult ; Biological Availability ; Chromatography, Liquid ; Eating ; Flavonoids/chemistry ; Flavonoids/metabolism ; Flavonoids/pharmacokinetics ; Food Handling ; Functional Food/analysis ; Humans ; Phenols/chemistry ; Phenols/metabolism ; Phenols/pharmacokinetics ; Pilot Projects ; Polyphenols ; Proanthocyanidins/chemistry ; Proanthocyanidins/metabolism ; Proanthocyanidins/pharmacokinetics ; Prunus/chemistry ; Spectrometry, Fluorescence ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Young Adult
Chemical Substances	Flavonoids ; Phenols ; Polyphenols ; Proanthocyanidins
Language	English
Publishing date	2010-09-01
Publishing country	United States
Document type	Controlled Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	523-x
ISSN	1096-0384 ; 0003-9861
ISSN (online)	1096-0384
ISSN	0003-9861
DOI	10.1016/j.abb.2010.03.020
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Article ; Online: Metabolomics study of human urinary metabolome modifications after intake of almond (Prunus dulcis (Mill.) D.A. Webb) skin polyphenols.

Llorach, Rafael / Garrido, Ignacio / Monagas, Maria / Urpi-Sarda, Mireia / Tulipani, Sara / Bartolome, Begona / Andres-Lacueva, Cristina

Journal of proteome research

2010 Volume 9, Issue 11, Page(s) 5859–5867

Abstract: Almond, as a part of the nut family, is an important source of biological compounds, and specifically, almond skins have been considered an important source of polyphenols, including flavan-3-ols and flavonols. Polyphenol metabolism may produce several ... ...

Abstract	Almond, as a part of the nut family, is an important source of biological compounds, and specifically, almond skins have been considered an important source of polyphenols, including flavan-3-ols and flavonols. Polyphenol metabolism may produce several classes of metabolites that could often be more biologically active than their dietary precursor and could also become a robust new biomarker of almond polyphenol intake. In order to study urinary metabolome modifications during the 24 h after a single dose of almond skin extract, 24 volunteers (n = 24), who followed a polyphenol-free diet for 48 h before and during the study, ingested a dietary supplement of almond skin phenolic compounds (n = 12) or a placebo (n = 12). Urine samples were collected before ((-2)-0 h) and after (0-2 h, 2-6 h, 6-10 h, and 10-24 h) the intake and were analyzed by liquid chromatography-mass spectrometry (LC-q-TOF) and multivariate statistical analysis (principal component analysis (PCA) and orthogonal projection to latent structures (OPLS)). Putative identification of relevant biomarkers revealed a total of 34 metabolites associated with the single dose of almond extract, including host and, in particular, microbiota metabolites. As far as we know, this is the first time that conjugates of hydroxyphenylvaleric, hydroxyphenylpropionic, and hydroxyphenylacetic acids have been identified in human samples after the consumption of flavan-3-ols through a metabolomic approach. The results showed that this non-targeted approach could provide new intake biomarkers, contributing to the development of the food metabolome as an important part of the human urinary metabolome.
MeSH term(s)	Biomarkers/urine ; Flavonoids/administration & dosage ; Flavonoids/metabolism ; Humans ; Metabolome ; Metabolomics/methods ; Phenols/administration & dosage ; Phenols/metabolism ; Polyphenols ; Prunus/metabolism ; Urine/chemistry
Chemical Substances	Biomarkers ; Flavonoids ; Phenols ; Polyphenols
Language	English
Publishing date	2010-11-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/pr100639v
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Article ; Online: Identification of an active RNAi pathway in

Iracane, Elise / Arias-Sardá, Cristina / Maufrais, Corinne / Ene, Iuliana V / d'Enfert, Christophe / Buscaino, Alessia

Proceedings of the National Academy of Sciences of the United States of America

2024 Volume 121, Issue 17, Page(s) e2315926121

Abstract: RNA interference (RNAi) is a fundamental regulatory pathway with a wide range of functions, including regulation of gene expression and maintenance of genome stability. Although RNAi is widespread in the fungal kingdom, well-known species, such as the ... ...

Abstract	RNA interference (RNAi) is a fundamental regulatory pathway with a wide range of functions, including regulation of gene expression and maintenance of genome stability. Although RNAi is widespread in the fungal kingdom, well-known species, such as the model yeast
MeSH term(s)	Humans ; Candida albicans/genetics ; RNA Interference ; Gene Editing ; Saccharomyces cerevisiae/metabolism ; Genomic Instability
Language	English
Publishing date	2024-04-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2315926121
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