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Article: Realizing the need for colorectal cancer screening in Pakistan.

Muhammad, Syed Zaki / Fatima, Noor

JPMA. The Journal of the Pakistan Medical Association

2020 Volume 70, Issue 7, Page(s) 1309

MeSH term(s)	Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/epidemiology ; Early Detection of Cancer ; Humans ; Mass Screening ; Pakistan/epidemiology
Language	English
Publishing date	2020-09-27
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	603873-6
ISSN	0030-9982
ISSN	0030-9982
DOI	10.5455/JPMA.65781
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Article: Myeloid Sarcoma - a diagnostic challenge in paediatric practice.

Nageen, Tabinda / Muhammad, Syed Zaki / Imam, Uzma

JPMA. The Journal of the Pakistan Medical Association

2022 Volume 71, Issue 12, Page(s) 2806–2808

Abstract: Myeloid Sarcomas are rare tumours of myeloid origin that may infiltrate multiple sites of the body. They may precede acute myeloid leukaemia or present without it. It has non-specific manifestations and presents as a diagnostic and therapeutic challenge ... ...

Abstract	Myeloid Sarcomas are rare tumours of myeloid origin that may infiltrate multiple sites of the body. They may precede acute myeloid leukaemia or present without it. It has non-specific manifestations and presents as a diagnostic and therapeutic challenge owing to the limited literature that reports consensus on diagnostic and treatment strategies. Immunohistochemistry is of significance in identifying the disease and acute myeloid leukaemia protocols of systemic therapy remain the mainstay of the treatment. Our report of an 11-year-old child with myeloid sarcoma aims to add to the limited existing literature and describe the varied presentation and sites of involvement.
Language	English
Publishing date	2022-02-12
Publishing country	Pakistan
Document type	Case Reports
ZDB-ID	603873-6
ISSN	0030-9982
ISSN	0030-9982
DOI	10.47391/JPMA.460
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Article ; Online: Poliovirus eradication initiatives in dire straits: Updates from Pakistan.

Shaikh, Nafhat / Fatima, Noor / Muhammad, Syed Zaki

Journal of global health

2021 Volume 11, Page(s) 3036

MeSH term(s)	Disease Eradication ; Humans ; Immunization Programs ; Pakistan/epidemiology ; Poliomyelitis/epidemiology ; Poliomyelitis/prevention & control ; Poliovirus ; Population Surveillance
Language	English
Publishing date	2021-03-01
Publishing country	Scotland
Document type	Journal Article ; Review
ZDB-ID	2741629-X
ISSN	2047-2986 ; 2047-2986
ISSN (online)	2047-2986
ISSN	2047-2986
DOI	10.7189/jogh.11.03036
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Article ; Online: The Constant Threat of Emerging Viral Diseases

Noor Fatima / Syed Zaki Muhammad

Liaquat National Journal of Primary Care, Vol 2, Iss 1, Pp 53-

2020 Volume 53

Abstract: Respected Editor, Sudden outbreaks of transpiring viral infections are putting human race into great danger. Different outbreaks in the past such as those of Influenza, Ebola, and Zika viruses and the most recent novel coronavirus, severe acute ... ...

Abstract	Respected Editor, Sudden outbreaks of transpiring viral infections are putting human race into great danger. Different outbreaks in the past such as those of Influenza, Ebola, and Zika viruses and the most recent novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continue to remind us of our constant vulnerability and warrant constant preparedness [1]. Emerging infections could be defined as the ones whose occurrence has increased in the last few years and threatens to increase in the for-coming years. Outbreaks of disease can be initiated by the transmission of a novel pathogen, or due to reemergence of a known pathogen [2]. Viral outbreaks are unpredictable because they result from multifaceted interaction between the virus, vector, host and environment [3]. Outbreaks are likely to cause fast transmission of disease which might cause high morbidity in those affected. SoutheastAsia is at a greater risk for the outbreak of pathogenic viruses [2]. Evolutionary changes in pathogens especially viral genetic change that allows cross-species transmission is the basis of zoonosis which puts humans at risk of infecting viruses of animal origin, such was the case in previous outbreaks [4]. This is aided by social and environmental factors - such as increasing global population, rapid urbanization, climate change and, increased susceptibility to infections due to an aging population worldwide - that favor incidence of new viral infections [2]. Quick and precise recognition of emergent pathogens is necessary for rapid countermeasures, better clinical management, and to reduce the extent of these viral infections. For detection, development of diagnostic tests that are cost effective, quick and error free, is essential [2]. One of the widely used molecular method is a polymerase chain reaction (PCR) for identification of viruses; it is rapid, cost-effective, and simple [4]. The capability of viruses to cross species barriers, acclimatize to different environments, and to resist existing ...
Keywords	Medicine (General) ; R5-920
Subject code	610
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	Liaquat National Hospital and Medical College
Document type	Article ; Online
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Article ; Online: Targeted Analysis of Veterinary Drugs in Food Samples by Developing a High-Resolution Tandem Mass Spectral Library.

Khadim, Adeeba / Yaseen Jeelani, Syed Usama / Khan, Muhammad Noman / Kumari, Sindhia / Raza, Ali / Ali, Arslan / Zareena, Bibi / Zaki Shah, Syed Muhammad / Musharraf, Syed Ghulam

Journal of agricultural and food chemistry

2023 Volume 71, Issue 34, Page(s) 12839–12848

Abstract: Veterinary drug residues present in foods can pose severe health threats to the population. The present study aims to develop a high-resolution mass spectral library of 158 veterinary drugs of 16 different classes for their rapid identification in food ... ...

Abstract	Veterinary drug residues present in foods can pose severe health threats to the population. The present study aims to develop a high-resolution mass spectral library of 158 veterinary drugs of 16 different classes for their rapid identification in food samples through liquid chromatography-high-resolution electrospray ionization-tandem mass spectrometry (LC-HR-ESI-MS/MS). Standard drugs were pooled according to their log
MeSH term(s)	Tandem Mass Spectrometry/methods ; Spectrometry, Mass, Electrospray Ionization/methods ; Veterinary Drugs/analysis ; Gas Chromatography-Mass Spectrometry/methods ; Chromatography, Liquid/methods ; Ions/chemistry ; Chromatography, High Pressure Liquid
Chemical Substances	Veterinary Drugs ; Ions
Language	English
Publishing date	2023-08-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c03715
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Article ; Online: Phytoassisted synthesis of CuO and Ag-CuO nanocomposite, characterization, chemical sensing of ammonia, degradation of methylene blue.

Farooq, Muhammad / Shujah, Shaukat / Tahir, Kamran / Hussain, Syed Tasleem / Khan, Afaq Ullah / Almarhoon, Zainab M / Alabbosh, Khulood Fahad / Alanazi, Abdulaziz A / Althagafi, Talal M / Zaki, Magdi E A

Scientific reports

2024 Volume 14, Issue 1, Page(s) 1618

Abstract: The elimination of hazardous industrial pollutants from aqueous solutions is an emerging area of scientific research and a worldwide problem. An efficient catalyst, Ag-CuO was synthesized for the degradation of methylene blue, the chemical sensing of ... ...

Abstract	The elimination of hazardous industrial pollutants from aqueous solutions is an emerging area of scientific research and a worldwide problem. An efficient catalyst, Ag-CuO was synthesized for the degradation of methylene blue, the chemical sensing of ammonia. A simple novel synthetic method was reported in which new plant material Capparis decidua was used for the reduction and stabilization of the synthesized nanocatalyst. A Varying amount of Ag was doped into CuO to optimize the best catalyst that met the required objectives. Through this, the Ag-CuO nanocomposite was characterized by XRD, SEM, HR-TEM, EDX, and FTIR techniques. The mechanism of increased catalytic activity with Ag doping involves the formation of charge sink and suppression of drop back probability of charge from conduction to valance band. Herein, 2.7 mol % Ag-CuO exhibited better catalytic activities and it was used through subsequent catalytic experiments. The experimental conditions such as pH, catalyst dose, analyte initial concentration, and contact time were optimized. The as-synthesized nanocomposite demonstrates an excellent degradation efficacy of MB which is 97% at pH 9. More interestingly, the as-synthesized catalyst was successfully applied for the chemical sensing of ammonia even at very low concentrations. The lower limit of detection (LLOD) also called analytic sensitivity was calculated for ammonia sensing and found to be 1.37 ppm.
Language	English
Publishing date	2024-01-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-51391-2
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Article ; Online: Significance and Challenges of Big Data in Healthcare

Syed Muhammad Nabeel Mustafa / Hassan Zaki / Syeda Sundus Zehra / Muhammad Shoaib

University of Sindh Journal of Information and Communication Technology, Vol 6, Iss 1, Pp 25-

A Review

2022 Volume 30

Abstract: The infiltration of information and technology in all the important sectors of the world has influenced the efflux of data. Healthcare industry has greatly benefited more the blessings of advanced computing. This sector is getting digitized at a fast ... ...

Abstract	The infiltration of information and technology in all the important sectors of the world has influenced the efflux of data. Healthcare industry has greatly benefited more the blessings of advanced computing. This sector is getting digitized at a fast rate for more precision and accuracy. Hence, more data is produced from the healthcare sector. In this review paper, we discuss Big data's importance in the healthcare industry. We go over the qualities of big data, including volume, veracity, speed, variety, and value. The healthcare industry has been touched by big data in practically every way. The diagnosis, telemedicine, medical research, and enhancing patient care are the remaining key effects. The application of Big data gave rise to many challenges such as accuracy, security and privacy, storage and processing, data complexity, unified format, image preprocessing and data analytics. We also intend to investigate further big data uses in healthcare and to highlight the difficulties this industry faces
Keywords	healthcare ; big data ; big data analytics ; medical research ; telemedicine ; Information technology ; T58.5-58.64 ; Electronic computers. Computer science ; QA75.5-76.95
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	University of Sindh
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Seizures and Basal Ganglia Calcification Caused by Hypoparathyroidism.

Nageen, Tabinda / Muhammad, Syed Zaki / Jamal, Ammarah

Cureus

2020 Volume 12, Issue 4, Page(s) e7888

Abstract: Seizures, not a typical feature of hypoparathyroidism, may present along with myalgia and tetany in patients of hypoparathyroidism. Thorough history and examination, derangement of biochemical parameters such as hypocalcemia, hyperphosphatemia, and ... ...

Abstract	Seizures, not a typical feature of hypoparathyroidism, may present along with myalgia and tetany in patients of hypoparathyroidism. Thorough history and examination, derangement of biochemical parameters such as hypocalcemia, hyperphosphatemia, and inappropriately low levels of parathyroid hormone, along with basal ganglia calcification as seen on imaging, lead to the diagnosis of hypoparathyroidism in a 10-year-old child who presented to us. Treatment with calcium and active vitamin D significantly improved his condition. In this report, we discuss the presentation and treatment of hypoparathyroidism. Early detection and follow-up at clinically appropriate intervals is important to avoid complications.
Language	English
Publishing date	2020-04-29
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.7888
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Sensitive Detection of Pharmaceutical Drugs and Metabolites in Serum Using Data-Independent Acquisition Mass Spectrometry and Open-Access Data Acquisition Tools

Syed Muhammad Zaki Shah / Arslan Ali / Muhammad Noman Khan / Adeeba Khadim / Mufarreh Asmari / Jalal Uddin / Syed Ghulam Musharraf

Pharmaceuticals, Vol 15, Iss 7, p

2022 Volume 901

Abstract: Data-independent acquisition (DIA) based strategies have been explored in recent years for improving quantitative analysis of metabolites. However, the data analysis is challenging for DIA methods as the resulting spectra are highly multiplexed. Thus, ... ...

Abstract	Data-independent acquisition (DIA) based strategies have been explored in recent years for improving quantitative analysis of metabolites. However, the data analysis is challenging for DIA methods as the resulting spectra are highly multiplexed. Thus, the DIA mode requires advanced software analysis to facilitate the data deconvolution process. We proposed a pipeline for quantitative profiling of pharmaceutical drugs and serum metabolites in DIA mode after comparing the results obtained from full-scan, Data-dependent acquisition (DDA) and DIA modes. using open-access software. Pharmaceutical drugs (10) were pooled in healthy human serum and analysed by LC-ESI-QTOF-MS. MS1 full-scan and Data-dependent (MS2) results were used for identification using MS-DIAL software while deconvolution of MS1/MS2 spectra in DIA mode was achieved by using Skyline software. The results of acquisition methods for quantitative analysis validated the remarkable analytical performance of the constructed workflow, proving it to be a sensitive and reproducible pipeline for biological complex fluids.
Keywords	metabolomics ; data-independent acquisition ; data-dependent acquisition ; MS-DIAL ; Skyline ; Perseus ; Medicine ; R ; Pharmacy and materia medica ; RS1-441
Subject code	006
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Untargeted screening of plant metabolites based on data-independent and data-dependent acquisition modes using LC-ESI-QTOF-MS

Syed Muhammad Zaki Shah / Muhammad Ramzan / Muhammad Noman Khan / Hamna Shadab / Muhammad Usman / Saeedur Rahman / Arslan Ali / Jalal Uddin / Mufarreh Asmari / Syed Ghulam Musharraf

Arabian Journal of Chemistry, Vol 16, Iss 8, Pp 104978- (2023)

Tribulus terrestris L. as a case study

2023

Abstract: Metabolomics has been used as a powerful tool for the analysis, and drug-lead identification in medicinal plants and herbal medicines. For the coverage of a broader range of plant-based metabolites using LC-MS, one of the important parameters is the ... ...

Abstract	Metabolomics has been used as a powerful tool for the analysis, and drug-lead identification in medicinal plants and herbal medicines. For the coverage of a broader range of plant-based metabolites using LC-MS, one of the important parameters is the selection of analysis mode and data processing for identification. This paper describes the utility of two distinctive acquisition modes in combination, a classic data-dependent acquisition (DDA) mode and a data-independent acquisition (DIA) mode for obtaining the mass spectrometry data of plant extracts using LC-ESI-QTOF/MS. Tribulus terrestris plant was used as a case study. We have applied three-step data analysis pipeline 1-annotation and putative identification of metabolites, 2-validation, and relative quantification, and 3-multivariate analysis using open-access MS-DIAL, Skyline, and Perseus software. A total of four samples of T. terrestris (aqueous extracts), two fruits, and two whole plant samples, from two different regions, were used. By combining data analysis results of plant fruit samples from two different regions, a total of 95 and 77 metabolites were identified in positive and negative ionization modes, respectively. Similarly, in the analysis of the whole plant from two different regions, 75 and 76 metabolites were identified in positive and negative ionization modes, respectively. We suggested the use of DDA mode for annotation, identification of metabolites, and generation of transition lists in MS-DIAL, furthermore, the use of DIA acquisition mode for enhancing metabolites sensitivity in complex samples, deconvolute MS1/MS2 spectra in Skyline for the quantitative performance and analytical reliability. The developed protocol can be used for the broader coverage of plant-based metabolites.
Keywords	Data-independent acquisition (DIA) ; MS-DIAL ; Skyline ; Tribulus terrestris ; Liquid-chromatography-mass-spectrometry (LC-MS) ; Chemistry ; QD1-999
Subject code	580
Language	English
Publishing date	2023-08-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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