LIVIVO - Search results -

Search results

Result 1 - 10 of total 71

Search options

Article ; Online: Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Antunes, Evelyn C. / Temmink, Hardy / Schuur, Boelo

Journal of Chemical Technology & Biotechnology. 2024 Jan., v. 99, no. 1 p.259-269

2024

Abstract: BACKGROUND: Natural polymers are macromolecules produced by living organisms, and they have a wide range of applications and relevance for the development of a circular economy. However, large‐scale production continues to be hindered by several factors, ...

Abstract	BACKGROUND: Natural polymers are macromolecules produced by living organisms, and they have a wide range of applications and relevance for the development of a circular economy. However, large‐scale production continues to be hindered by several factors, such as downstream processing. In this work, three‐phase partitioning (TPP) systems were investigated for separation of model polysaccharide (dextran, alginate, and gum arabic) from protein [Bovine serum albumin (BSA) and lysozyme]. The recyclability of the phase‐forming compounds used to form the extractive platform was assessed by ultrafiltration (UF). This study contributes to the development of production processes for biopolymers from fermented waste by proposing an effective separation technique for fractionation of biopolymers. Such biopolymers are often collected as mixtures, but with the studied approaches, fractionation of polysaccharides from proteins may also be employed. With the chosen systems, the scope of TPP systems is expanded by using another class of phase‐forming compound (polymers); in addition, UF was studied as a versatile regeneration approach. RESULTS: Within the TPP approach, the best separation of dextran from BSA was achieved using TPP systems composed of 25 wt% polyethylene glycol (PEG) + 25 wt% K₃C₆H₅O₇ and 36 wt% EtOH + 10 wt% K₃PO₄, in which more than 95% of dextran and BSA were found as precipitate and partitioned to top phase (PEG or EtOH‐rich), respectively. By using other model compounds, it was found that the molecular weight and charge of the biopolymer play a key role in the yield and selectivity of TPP systems. Finally, by using ultrafiltration/diafiltration, about 99% of the ethanol and phosphate salt used to form the extractive platform could be retrieved in the permeate stream. CONCLUSION: The high extraction yields, good selectivity, and recyclability of phase‐forming compounds confirm the potential of polymer‐based and alcohol‐based TPP systems to fractionate biopolymer mixtures. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Keywords	alginates ; biopolymers ; biotechnology ; bovine serum albumin ; circular economy ; dextran ; ethanol ; fermentation ; fractionation ; gum arabic ; lysozyme ; molecular weight ; phosphates ; polyethylene glycol ; ultrafiltration ; wastes
Language	English
Dates of publication	2024-01
Size	p. 259-269.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	1479465-2
ISSN	1097-4660 ; 0268-2575
ISSN (online)	1097-4660
ISSN	0268-2575
DOI	10.1002/jctb.7534
Database	NAL-Catalogue (AGRICOLA)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Separation of polysaccharide and protein by ionic liquid-based extraction techniques

Evelyn C. Antunes / Felipe Oliveira / Hardy Temmink / Boelo Schuur

Journal of Ionic Liquids, Vol 3, Iss 2, Pp 100058- (2023)

2023

Abstract: Biopolymers are natural macromolecules obtained from animal, plant and microbial sources, with the potential to be used in a wide range of applications. A key process step, which is still underdeveloped, is the downstream processing. In this work, water ... ...

Abstract	Biopolymers are natural macromolecules obtained from animal, plant and microbial sources, with the potential to be used in a wide range of applications. A key process step, which is still underdeveloped, is the downstream processing. In this work, water immiscible and water miscible ionic liquids (ILs) were investigated regarding their ability to fractionate a mixture of polysaccharide and proteins. Alginate and bovine serum albumin (BSA) were used as model compounds to mimic natural polymer crude extract. Phosphonium ILs composed of different anions (bromide, dicyanamide and phosphinate) were used as water immiscible ILs while imidazolium ILs, combined with phosphate salts to form biphasic system, were selected as water miscible ILs. In water immiscible IL systems, the partitioning behavior of biopolymers depended on IL's anions and there was formation of insoluble precipitate. The insolubility of precipitate in diverse aqueous and organic solvents hindered the processibility of water immiscible phosphonium IL for fractionation of biopolymers. The partitioning of biopolymers in water miscible ILs systems also depended on the IL's anion, as well the concentration of IL. Separation of alginate (yield = 90% and purity = 99%) from BSA (yield = 89% and purity = 99%) was best achieved by the [C4mim]Cl-based extraction system. After fractionation, regeneration of IL and salt used was carried out by ultrafiltration, with recovery yields up to 100%. The high extraction yields and recyclability of phase-forming compounds confirm the potential of water miscible ILs systems to fractionate polysaccharide and protein.
Keywords	Polysaccharide ; Protein ; Ionic liquids ; Aqueous two-phase system ; Fractionation ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: High-throughput Soxhlet extraction method applied for analysis of leaf lignocellulose and non-structural substances.

Torres-Rodriguez, Alejandra / Darvishzadeh, Roshanak / Skidmore, Andrew K / Fränzel-Luiten, Erna / Knaken, Benno / Schuur, Boelo

MethodsX

2024 Volume 12, Page(s) 102644

Abstract: The traditional Soxhlet extraction method is commonly employed to extract soluble components from non-soluble components in a solid matrix, for example, non-structural substances in biomass samples that can be separated from structural lignocellulosic ... ...

Abstract	The traditional Soxhlet extraction method is commonly employed to extract soluble components from non-soluble components in a solid matrix, for example, non-structural substances in biomass samples that can be separated from structural lignocellulosic compounds in biomass samples. Conventional laboratory procedures for such extractions typically involve a low sample throughput, with each run being performed individually, resulting in time-consuming and labour-intensive processes, making them impractical for analysing large sample sets. In research fields such as Earth Observation in Forest Ecosystems, extensive fieldwork sampling is required across large study areas, resulting in a substantial number of leaf samples, each with limited mass. In this study, an innovative adaptation of the conventional National Renewable Energy Laboratory (NREL) Soxhlet method is developed to create a high-throughput mini-Soxhlet apparatus that enables the simultaneous extraction of up to nineteen samples, each with a mass of 0.3 g per sample. With this adaptation, we measured the lignocellulose and extractive in 343 leaf samples collected from four temperate forest tree species. This modified approach enhances versatility and can be applied to all solid-liquid extractions and various types of vegetation tissues, such as tree leaves, shrubs, crops, feedstock, and other non-woody samples.•The solid-liquid extraction method has been implemented in a heating block facilitating 19 small flasks to measure multiple samples simultaneously while requiring only a small sample mass.•The apparatus set-up was constructed using an alumina heating block mounted on a standard laboratory heating plate. Boiling flask tubes were placed in the heating block and equipped with condenser caps and filters on glass rods on which the solid samples were placed.•The adjustments made the method suitable for application to diverse vegetation tissues and non-woody sample types. It holds particular appeal for research areas that necessitate a high sample number.
Language	English
Publishing date	2024-03-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2830212-6
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2024.102644
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Recent Developments in Solvent-Based Fluid Separations.

Schuur, Boelo / Brouwer, Thomas / Sprakel, Lisette M J

Annual review of chemical and biomolecular engineering

2021 Volume 12, Page(s) 573–591

Abstract: The most important developments in solvent-based fluid separations, separations involving at least one fluid phase, are reviewed. After a brief introduction and discussion on general solvent trends observed in all fields of application, several specific ... ...

Abstract	The most important developments in solvent-based fluid separations, separations involving at least one fluid phase, are reviewed. After a brief introduction and discussion on general solvent trends observed in all fields of application, several specific fields are discussed. Important solvent trends include replacement of traditional molecular solvents by ionic liquids and deep eutectic solvents and, more recently, increasing discussion around bio-based solvents in some application fields. Furthermore, stimuli-responsive systems are discussed; the most significant developments in this field are seen for CO
MeSH term(s)	Ionic Liquids ; Solvents
Chemical Substances	Ionic Liquids ; Solvents
Language	English
Publishing date	2021-04-14
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2516755-8
ISSN	1947-5446 ; 1947-5438
ISSN (online)	1947-5446
ISSN	1947-5438
DOI	10.1146/annurev-chembioeng-102620-015346
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Antunes, Evelyn / Temmink, Hardy / Schuur, Boelo

Journal of Chemical Technology and Biotechnology

2023 Volume 99, Issue 1

Abstract: Natural polymers are macromolecules produced by living organisms, and they have a wide range of applications and relevance for the development of a circular economy. However, large-scale production continues to be hindered by several factors, such as ... ...

Abstract	Natural polymers are macromolecules produced by living organisms, and they have a wide range of applications and relevance for the development of a circular economy. However, large-scale production continues to be hindered by several factors, such as downstream processing. In this work, three-phase partitioning (TPP) systems were investigated for separation of model polysaccharide (dextran, alginate, and gum arabic) from protein [Bovine serum albumin (BSA) and lysozyme]. The recyclability of the phase-forming compounds used to form the extractive platform was assessed by ultrafiltration (UF). This study contributes to the development of production processes for biopolymers from fermented waste by proposing an effective separation technique for fractionation of biopolymers. Such biopolymers are often collected as mixtures, but with the studied approaches, fractionation of polysaccharides from proteins may also be employed. With the chosen systems, the scope of TPP systems is expanded by using another class of phase-forming compound (polymers); in addition, UF was studied as a versatile regeneration approach.Within the TPP approach, the best separation of dextran from BSA was achieved using TPP systems composed of 25 wt% polyethylene glycol (PEG) + 25 wt% K3C6H5O7 and 36 wt% EtOH + 10 wt% K3PO4, in which more than 95% of dextran and BSA were found as precipitate and partitioned to top phase (PEG or EtOH-rich), respectively. By using other model compounds, it was found that the molecular weight and charge of the biopolymer play a key role in the yield and selectivity of TPP systems. Finally, by using ultrafiltration/diafiltration, about 99% of the ethanol and phosphate salt used to form the extractive platform could be retrieved in the permeate stream.The high extraction yields, good selectivity, and recyclability of phase-forming compounds confirm the potential of polymer-based and alcohol-based TPP systems to fractionate biopolymer mixtures
Keywords	Life Science
Subject code	540
Language	English
Publishing country	nl
Document type	Article ; Online
ZDB-ID	1479465-2
ISSN	1097-4660 ; 0268-2575
ISSN (online)	1097-4660
ISSN	0268-2575
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Order via subito

Details ▾
- Full text online
- Order with fees

Article: Comparison of solvent‐based affinity separation processes using Cyrene and Sulfolane for aromatic/aliphatic separations

Brouwer, Thomas / Schuur, Boelo

Journal of chemical technology and biotechnology. 2021 Sept., v. 96, no. 9

2021

Abstract: BACKGROUND: Vapor–liquid and liquid–liquid equilibria of unsaturated and saturated hydrocarbons with the bio‐based solvent dihydrolevoglucosenone, trademarked as Cyrene, have been reported recently, aiming at the utilization of the solvent in liquid– ... ...

Abstract	BACKGROUND: Vapor–liquid and liquid–liquid equilibria of unsaturated and saturated hydrocarbons with the bio‐based solvent dihydrolevoglucosenone, trademarked as Cyrene, have been reported recently, aiming at the utilization of the solvent in liquid–liquid extraction (LLX) and extractive distillation (ED). In this work, for a model system comprised of methylcyclohexane (MCH) and toluene (TOL), both LLX‐based and ED‐based processes were compared over a wide range of compositions based on simulations in AspenPlus V10. An economic evaluation based on total annual costs was performed, and the processes using Cyrene were compared with equivalent processes using the industrial benchmark Sulfolane. RESULTS: In the absence of literature data for Sulfolane–MCH–TOL, additional liquid–liquid extractions were done to facilitate parameter estimation for simulation. The Cyrene‐based ED process was found to be more efficient than the Sulfolane‐based ED process, primarily at lower (<50 mol%) TOL feed concentration, but also higher TOL feed concentrations. For LLX purposes it was found that the Cyrene‐based LLX process was economically the least attractive, primarily due to the larger miscibility region of Cyrene with the hydrocarbon mixture. CONCLUSIONS: The overall conclusion for this specific binary mixture is that LLX with Sulfolane is most economic at a TOL feed concentration of <30 mol%, while for >30 mol% the ED process with Cyrene is most economic. This process analysis showed that Cyrene is an appropriate bio‐based alternative for Sulfolane as an entrainer for ED processes to separate aromatics and aliphatics. © 2021 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Keywords	biotechnology ; distillation ; economic evaluation ; liquid-liquid extraction ; solvents ; toluene
Language	English
Dates of publication	2021-09
Size	p. 2630-2646.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1479465-2
ISSN	1097-4660 ; 0268-2575
ISSN (online)	1097-4660
ISSN	0268-2575
DOI	10.1002/jctb.6808
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Delignification of Low-Energy Mechanical Pulp (Asplund Fibers) in a Deep Eutectic Solvent System of Choline Chloride and Lactic Acid.

Pérez, Alan D / Fiskari, Juha / Schuur, Boelo

Frontiers in chemistry

2021 Volume 9, Page(s) 688291

Abstract: Deep eutectic solvents (DESs) are considered as a green and environmentally benign solvent class for various applications, including delignification of biomass. One of the major challenges in the delignification of biomass by DES is attributed to the ... ...

Abstract	Deep eutectic solvents (DESs) are considered as a green and environmentally benign solvent class for various applications, including delignification of biomass. One of the major challenges in the delignification of biomass by DES is attributed to the limitations in mass transfer. By subjecting wood chips to a low-energy mechanical refining, i.e., the Asplund process, the accessible surface area increases greatly, which in turn improves the mass transfer and increases the reaction rate. In this research, the DES delignification of Asplund fibers made of Norway spruce was studied as a strategy to produce papermaking fibers under mild conditions. A DES consisting of lactic acid and choline chloride was used due to its proven performance in delignification. Various operational conditions, such as temperature, time, DES-to-wood ratio, and the type of stirring were studied. A novel parameter,
Language	English
Publishing date	2021-06-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711776-5
ISSN	2296-2646
ISSN	2296-2646
DOI	10.3389/fchem.2021.688291
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Recovery Techniques Enabling Circular Chemistry from Wastewater.

Elhami, Vahideh / Antunes, Evelyn C / Temmink, Hardy / Schuur, Boelo

Molecules (Basel, Switzerland)

2022 Volume 27, Issue 4

Abstract: In an era where it becomes less and less accepted to just send waste to landfills and release wastewater into the environment without treatment, numerous initiatives are pursued to facilitate chemical production from waste. This includes microbial ... ...

Abstract	In an era where it becomes less and less accepted to just send waste to landfills and release wastewater into the environment without treatment, numerous initiatives are pursued to facilitate chemical production from waste. This includes microbial conversions of waste in digesters, and with this type of approach, a variety of chemicals can be produced. Typical for digestion systems is that the products are present only in (very) dilute amounts. For such productions to be technically and economically interesting to pursue, it is of key importance that effective product recovery strategies are being developed. In this review, we focus on the recovery of biologically produced carboxylic acids, including volatile fatty acids (VFAs), medium-chain carboxylic acids (MCCAs), long-chain dicarboxylic acids (LCDAs) being directly produced by microorganisms, and indirectly produced unsaturated short-chain acids (USCA), as well as polymers. Key recovery techniques for carboxylic acids in solution include liquid-liquid extraction, adsorption, and membrane separations. The route toward USCA is discussed, including their production by thermal treatment of intracellular polyhydroxyalkanoates (PHA) polymers and the downstream separations. Polymers included in this review are extracellular polymeric substances (EPS). Strategies for fractionation of the different fractions of EPS are discussed, aiming at the valorization of both polysaccharides and proteins. It is concluded that several separation strategies have the potential to further develop the wastewater valorization chains.
Language	English
Publishing date	2022-02-18
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27041389
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.MO 81: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020.

Falconer, Robert J / Schuur, Boelo / Mittermaier, Anthony K

Journal of molecular recognition : JMR

2021 Volume 34, Issue 10, Page(s) e2901

Abstract: The last 5 years have seen a series of advances in the application of isothermal titration microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in understanding multiprotein complex formation including proteolysis- ... ...

Abstract	The last 5 years have seen a series of advances in the application of isothermal titration microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in understanding multiprotein complex formation including proteolysis-targeting chimeras (PROTACS), and mitochondrial autophagy receptor Nix interaction with LC3 and GABARAP. It has also helped elucidate complex allosteric communication in protein complexes like trp RNA-binding attenuation protein (TRAP) complex. Advances in kinetics analysis have enabled the calculation of kinetic rate constants from pre-existing ITC data sets. Diverse strategies have also been developed to study enzyme kinetics and enzyme-inhibitor interactions. ITC has also been applied to study small molecule solvent and solute interactions involved in extraction, separation, and purification applications including liquid-liquid separation and extractive distillation. Diverse applications of ITC have been developed from the analysis of protein instability at different temperatures, determination of enzyme kinetics in suspensions of living cells to the adsorption of uremic toxins from aqueous streams.
MeSH term(s)	Animals ; Biomedical Research/methods ; Calorimetry/instrumentation ; Calorimetry/methods ; Catalysis ; Drug Discovery/methods ; Entropy ; Enzymes/chemistry ; Enzymes/metabolism ; Humans ; Liquid-Liquid Extraction/methods ; Minerals/chemistry ; Minerals/isolation & purification ; Proteins/chemistry ; Uremic Toxins/chemistry ; Uremic Toxins/isolation & purification
Chemical Substances	Enzymes ; Minerals ; Proteins ; Uremic Toxins
Language	English
Publishing date	2021-05-11
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1015084-5
ISSN	1099-1352 ; 0952-3499
ISSN (online)	1099-1352
ISSN	0952-3499
DOI	10.1002/jmr.2901
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2954: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Recovery Techniques Enabling Circular Chemistry from Wastewater

Vahideh Elhami / Evelyn C. Antunes / Hardy Temmink / Boelo Schuur

Molecules, Vol 27, Iss 1389, p

2022 Volume 1389

Abstract	In an era where it becomes less and less accepted to just send waste to landfills and release wastewater into the environment without treatment, numerous initiatives are pursued to facilitate chemical production from waste. This includes microbial conversions of waste in digesters, and with this type of approach, a variety of chemicals can be produced. Typical for digestion systems is that the products are present only in (very) dilute amounts. For such productions to be technically and economically interesting to pursue, it is of key importance that effective product recovery strategies are being developed. In this review, we focus on the recovery of biologically produced carboxylic acids, including volatile fatty acids (VFAs), medium-chain carboxylic acids (MCCAs), long-chain dicarboxylic acids (LCDAs) being directly produced by microorganisms, and indirectly produced unsaturated short-chain acids (USCA), as well as polymers. Key recovery techniques for carboxylic acids in solution include liquid-liquid extraction, adsorption, and membrane separations. The route toward USCA is discussed, including their production by thermal treatment of intracellular polyhydroxyalkanoates (PHA) polymers and the downstream separations. Polymers included in this review are extracellular polymeric substances (EPS). Strategies for fractionation of the different fractions of EPS are discussed, aiming at the valorization of both polysaccharides and proteins. It is concluded that several separation strategies have the potential to further develop the wastewater valorization chains.
Keywords	extracellular polymeric substances ; long-chain dicarboxylic acids ; medium-chain carboxylic acids ; separation technology ; unsaturated fatty acids ; volatile fatty acids ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED