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Article ; Online: A Medium for Presumptive Identification of Vibrio parahaemolyticus.

Kaper, J B / Remmers, E F / Colwell, R R

2019 Volume 44, Issue 5, Page(s) 336

Language	English
Publishing date	2019-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	243284-5
ISSN	1944-9097 ; 0362-028X
ISSN (online)	1944-9097
ISSN	0362-028X
DOI	10.4315/0362-028X-44.5.336
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Article ; Online: A Medium for Presumptive Identification of Vibrio parahaemolyticus.

Kaper, J B / Remmers, E F / Colwell, R R

Journal of food protection

2019 Volume 43, Issue 12, Page(s) 936–938

Abstract: A medium has been devised for rapid, presumptive identification of Vibrio parahaemolyticus . Fermentation of mannitol, sucrose and lactose, arginine dihydrolase, production of indole, gas from carbohydrate and ... ...

Abstract	A medium has been devised for rapid, presumptive identification of Vibrio parahaemolyticus . Fermentation of mannitol, sucrose and lactose, arginine dihydrolase, production of indole, gas from carbohydrate and H
Language	English
Publishing date	2019-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	243284-5
ISSN	1944-9097 ; 0362-028X
ISSN (online)	1944-9097
ISSN	0362-028X
DOI	10.4315/0362-028X-43.12.936
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Article: Cross-Talk between Probiotic Nissle 1917 and Human Colonic Epithelium Affects the Metabolite Composition and Demonstrates Host Antibacterial Effect.

Dokladny, Karol / Crane, John K / Kassicieh, Alex J / Kaper, James B / Kovbasnjuk, Olga

Metabolites

2021 Volume 11, Issue 12

Abstract: Colonic epithelium-commensal interactions play a very important role in human health and disease development. Colonic mucus serves as an ecologic niche for a myriad of commensals and provides a physical barrier between the epithelium and luminal content, ...

Abstract	Colonic epithelium-commensal interactions play a very important role in human health and disease development. Colonic mucus serves as an ecologic niche for a myriad of commensals and provides a physical barrier between the epithelium and luminal content, suggesting that communication between the host and microbes occurs mainly by soluble factors. However, the composition of epithelia-derived metabolites and how the commensal flora influences them is less characterized. Here, we used mucus-producing human adult stem cell-derived colonoid monolayers exposed apically to probiotic
Language	English
Publishing date	2021-12-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo11120841
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Article ; Online: Component placement accuracy in two generations of handheld robotics-assisted knee arthroplasty.

Sicat, Chelsea S / Chow, James C / Kaper, Bertrand / Mitra, Riddhit / Xie, Jing / Schwarzkopf, Ran

Archives of orthopaedic and trauma surgery

2021 Volume 141, Issue 12, Page(s) 2059–2067

Abstract: Introduction: Total knee arthroplasty (TKA) is the gold standard for treatment of end-stage osteoarthritis. Previous studies have shown that successful outcomes following TKA depend on accurate implant alignment and soft tissue balancing. Robotic- ... ...

Abstract	Introduction: Total knee arthroplasty (TKA) is the gold standard for treatment of end-stage osteoarthritis. Previous studies have shown that successful outcomes following TKA depend on accurate implant alignment and soft tissue balancing. Robotic-assisted TKA have demonstrated improved accuracy in component placement and have been associated with better outcomes and patient satisfaction. This study aims to report on the execution accuracy of two generations of handheld robotic-assisted surgical systems. Methods: This was a retrospective analysis of TKA procedures with two sequential generations of the same handheld robotic-assisted surgical system. Intra-operative data captured included pre-operative limb deformity, limb axes, range of motion, kinematic balance, and the resulting plan for component placement in three-dimensional space. Patients were stratified based on their preoperative coronal lower limb mechanical alignment (> 3° varus, < 3° varus, < 3° valgus, and ≥ 3° valgus). Measurements of component placement (overall lower limb alignment, medial and lateral flexion gaps, and tibial and femoral resection depths) were assessed using descriptive statistics. Results: A total of 435 patients were included and stratified based on preoperative lower limb alignment: 229 with ≥ 3° varus, 78 with varus < 3° and 58 with valgus < 3°, and 70 with valgus > 3°. The mean difference between planned and achieved alignment in the lower limb valgus patients was < 1° across all groups. Mean differences between planned and achieved medial flexion gap was higher in the > 3° subgroup in the varus patient cohort ([< 3°]: 1.15 ± 1.92 vs. [> 3°]: 1.90 ± 2.57); this value was higher in the < 3° subgroup for valgus patients ([< 3°]: 1.34 ± 1.83 vs. [> 3°]: 0.956 ± 1.65). Average resection depth ranged from 9.46 to 10.4 mm in the posterior medial femur, 9.25 to 9.95 mm in the posterior lateral femur, 7.45 to 8.79 mm in the distal medial femur, 8.22 to 9.18 mm in the distal lateral femur, 6.70 to 7.07 mm in the medial tibial condyle and 6.40 to 7.19 mm in the lateral tibial condyle. Non-inferiority testing demonstrated the newer generation is non-inferior to the older generation. Conclusion: Robotic-assisted knee replacement using handheld image-free systems is able to maintain accuracy of component placement. Further investigation of patient reported outcomes as well as long-term implant longevity are needed.
MeSH term(s)	Arthroplasty, Replacement, Knee ; Femur/surgery ; Humans ; Knee Joint/diagnostic imaging ; Knee Joint/surgery ; Knee Prosthesis ; Osteoarthritis, Knee/surgery ; Range of Motion, Articular ; Retrospective Studies ; Tibia/surgery
Language	English
Publishing date	2021-07-25
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	80407-1
ISSN	1434-3916 ; 0003-9330 ; 0344-8444
ISSN (online)	1434-3916
ISSN	0003-9330 ; 0344-8444
DOI	10.1007/s00402-021-04040-6
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Article ; Online: A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens.

Wan, Hongping / Ren, Ke / Kaper, Hans J / Sharma, Prashant K

Colloids and surfaces. B, Biointerfaces

2020 Volume 193, Page(s) 110977

Abstract: Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function. Biolubrication gets impaired due to diseases ... ...

Abstract	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function. Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition. Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary. Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C). Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties. Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03. At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07 ± 0.04 (healthy tissue) to 0.15 ± 0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50 min. After Chi-C treatment of damaged cartilage, the COF fell to 0.06 ± 0.03, which is comparable to healthy cartilage. Chi-C did not adversely affect the metabolic activity of human chondrocytes. This study provides new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules.
MeSH term(s)	Animals ; Cartilage, Articular/metabolism ; Catechols/chemistry ; Catechols/metabolism ; Cattle ; Chitosan/chemistry ; Chitosan/metabolism ; Chondrocytes/metabolism ; Chondroitinases and Chondroitin Lyases/metabolism ; Humans ; Lubrication ; Microscopy, Atomic Force ; Particle Size ; Surface Properties ; Synovial Fluid/chemistry ; Synovial Fluid/metabolism
Chemical Substances	Catechols ; Chitosan (9012-76-4) ; Chondroitinases and Chondroitin Lyases (EC 4.2.2.-) ; catechol (LF3AJ089DQ)
Keywords	covid19
Language	English
Publishing date	2020-04-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2020.110977
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Article: A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens

Wan, Hongping / Ren, Ke / Kaper, Hans J / Sharma, Prashant K

Colloids Surf B Biointerfaces

Abstract	Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function. Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage results from alterations in synovial fluid and lamina splendens composition. Osteoarthritis is treated with hyaluronic acid (HA) orally or via intra-articular injection, but due to the poor adsorption of HA on the cartilage surface in the absence of adhesive molecules, pain relief is temporary. Here, we describe how natural lubrication on degraded cartilage surface can be restored with the help of a bioinspired mucoadhesive biopolymer chitosan catechol (Chi-C). Quartz crystal microbalance was used to mimic the formation of lamina splendens in vitro, known as synovial fluid conditioning films (SyCF), and colloidal probe atomic force microscopy was used to measure their nanoscale frictional properties. Clear evidence of glycoprotein (PRG4) recruitment by Chi-C increased the softness of SyCF, which also improved nanoscale lubrication in vitro, decreasing the friction coefficient from 0.06 to 0.03. At the macroscale, cartilage damage induced by Chondroitinase ABC increased the coefficient of friction (COF) from 0.07â¯±â¯0.04 (healthy tissue) to 0.15â¯±â¯0.03 (after tissue damage) in the presence of synovial fluid after sliding for 50â¯min. After Chi-C treatment of damaged cartilage, the COF fell to 0.06â¯±â¯0.03, which is comparable to healthy cartilage. Chi-C did not adversely affect the metabolic activity of human chondrocytes. This study provides new key insight into the potential for restoring biolubrication through the use of muco-adhesive molecules.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #32408255
Database	COVID19

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Article ; Online: Phosphodiesterase 5 (PDE5) restricts intracellular cGMP accumulation during enterotoxigenic

Foulke-Abel, Jennifer / Yu, Huimin / Sunuwar, Laxmi / Lin, Ruxian / Fleckenstein, James M / Kaper, James B / Donowitz, Mark

Gut microbes

2020 Volume 12, Issue 1, Page(s) 1752125

Abstract: Diarrhea caused by ... ...

Abstract	Diarrhea caused by enterotoxigenic
MeSH term(s)	Bacterial Toxins/metabolism ; Caco-2 Cells ; Cell Line, Tumor ; Cyclic GMP/metabolism ; Cyclic Nucleotide Phosphodiesterases, Type 5/metabolism ; Diarrhea/microbiology ; Diarrhea/pathology ; Enterotoxigenic Escherichia coli/metabolism ; Enterotoxins/metabolism ; Epithelial Cells/metabolism ; Escherichia coli Infections/pathology ; Escherichia coli Proteins/metabolism ; Fimbriae Proteins/metabolism ; Humans ; Jejunum/microbiology ; Jejunum/pathology ; Membrane Glycoproteins/metabolism ; Peptide Hydrolases/metabolism ; Propionates/pharmacology ; Quinolines/pharmacology ; Virulence Factors/metabolism
Chemical Substances	Bacterial Toxins ; CfaE protein, E coli ; Enterotoxins ; Escherichia coli Proteins ; EtpA protein, E coli ; Membrane Glycoproteins ; Propionates ; Quinolines ; Virulence Factors ; heat stable toxin (E coli) ; Fimbriae Proteins (147680-16-8) ; verlukast (5Q9O54P0H7) ; heat-labile enterotoxin, E coli (D9K3SN2LNY) ; Cyclic Nucleotide Phosphodiesterases, Type 5 (EC 3.1.4.35) ; EatA protein, E coli (EC 3.4.-) ; Peptide Hydrolases (EC 3.4.-) ; Cyclic GMP (H2D2X058MU)
Language	English
Publishing date	2020-05-07
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2575755-6
ISSN	1949-0984 ; 1949-0984
ISSN (online)	1949-0984
ISSN	1949-0984
DOI	10.1080/19490976.2020.1752125
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Vibrios in the Louisiana gulf coast environment.

Roberts, N C / Siebeling, R J / Kaper, J B / Bradford, H B

Microbial ecology

2013 Volume 8, Issue 4, Page(s) 299–312

Abstract: A polyphasic approach, using bacteriological, immunological, and molecular biological techniques was used to elucidate the distribution of pathogenicVibrio species in the Louisiana coastal environment. A variety ofVibrio species pathogenic for man, ... ...

Abstract	A polyphasic approach, using bacteriological, immunological, and molecular biological techniques was used to elucidate the distribution of pathogenicVibrio species in the Louisiana coastal environment. A variety ofVibrio species pathogenic for man, includingV. cholerae, V. parahaemolyticus, V. fluvialis, andV. vulnificus, were found to be ubiquitous in Louisiana.Vibrio species monitored were shown to fluctuate in response to environmental factors of temperature, salinity, and nutrient level, and to vary independently of fecal coliform counts. A comprehensive serological screening system, based on species specific H antigens, was developed to identify pathogenicVibrio sp. 1 step after primary isolation.Vibrio sp. were correctly identified with accuracies ranging from 93-100%, depending on the specific H antiserum. Over 2,500V. cholerae isolates were rapidly screened for production of cholera toxin by DNA hybridization of specific toxin gene probes to colonies inoculated on nitrocellulose filter paper. The toxin gene probes, together with O antigen analysis, revealed that enterotoxigenicV. cholerae 01 serovars were recovered only from sewage stations or human disease, whereas enterotoxigenicV. cholerae non 01 serovars were recovered from environmental samples in addition to clinical and sewage samples. The results of this study indicate that techniques of immunology and molecular biology are very valuable supplements to conventional bacteriological techniques in studying the epidemiology and ecology of pathogenicVibrio sp.
Language	English
Publishing date	2013-11-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	1462065-0
ISSN	1432-184X ; 0095-3628
ISSN (online)	1432-184X
ISSN	0095-3628
DOI	10.1007/BF02010670
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Article: The locus of enterocyte effacement pathogenicity island of Shiga toxin-producing Escherichia coli O157:H7 and other attaching and effacing E. coli.

Kaper, J B

Japanese journal of medical science & biology

1998 Volume 51 Suppl, Page(s) S101–7

MeSH term(s)	Animals ; Bacterial Adhesion ; Bacterial Toxins/biosynthesis ; Epithelial Cells/microbiology ; Escherichia coli/genetics ; Escherichia coli/pathogenicity ; Escherichia coli/physiology ; Escherichia coli O157/genetics ; Escherichia coli O157/pathogenicity ; Escherichia coli O157/physiology ; Genes, Bacterial ; Humans ; Intestines/microbiology ; Shiga Toxins ; Virulence/genetics
Chemical Substances	Bacterial Toxins ; Shiga Toxins
Language	English
Publishing date	1998
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	218225-7
ISSN	1884-2828 ; 0021-5112
ISSN (online)	1884-2828
ISSN	0021-5112
DOI	10.7883/yoken1952.51.supplement1_s101
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Article: EPEC delivers the goods.

Kaper, J B

Trends in microbiology

1998 Volume 6, Issue 5, Page(s) 169–72; discussion 172–3

MeSH term(s)	Adhesins, Bacterial ; Animals ; Bacterial Adhesion ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Carrier Proteins ; Diarrhea/microbiology ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Infections/metabolism ; Escherichia coli Infections/microbiology ; Escherichia coli Proteins ; Humans ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism
Chemical Substances	Adhesins, Bacterial ; Bacterial Outer Membrane Proteins ; Bacterial Proteins ; Carrier Proteins ; Escherichia coli Proteins ; Receptors, Cell Surface ; Tir protein, E coli ; eaeA protein, E coli (147094-99-3)
Language	English
Publishing date	1998-05
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S. ; Review
ZDB-ID	1158963-2
ISSN	1878-4380 ; 0966-842X
ISSN (online)	1878-4380
ISSN	0966-842X
DOI	10.1016/s0966-842x(98)01266-9
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