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Article ; Online: The Arabidopsis APOLO and human UPAT sequence-unrelated long noncoding RNAs can modulate DNA and histone methylation machineries in plants.

Fonouni-Farde, Camille / Christ, Aurélie / Blein, Thomas / Legascue, María Florencia / Ferrero, Lucía / Moison, Michaël / Lucero, Leandro / Ramírez-Prado, Juan Sebastián / Latrasse, David / Gonzalez, Daniel / Benhamed, Moussa / Quadrana, Leandro / Crespi, Martin / Ariel, Federico

Genome biology

2022 Volume 23, Issue 1, Page(s) 181

Abstract: Background: RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene ... ...

Abstract	Background: RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene transcriptional activity. Results: Here, we show that in addition to the PRC1-component LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), APOLO interacts with the methylcytosine-binding protein VARIANT IN METHYLATION 1 (VIM1), a conserved homolog of the mammalian DNA methylation regulator UBIQUITIN-LIKE CONTAINING PHD AND RING FINGER DOMAINS 1 (UHRF1). The APOLO-VIM1-LHP1 complex directly regulates the transcription of the auxin biosynthesis gene YUCCA2 by dynamically determining DNA methylation and H3K27me3 deposition over its promoter during the plant thermomorphogenic response. Strikingly, we demonstrate that the lncRNA UHRF1 Protein Associated Transcript (UPAT), a direct interactor of UHRF1 in humans, can be recognized by VIM1 and LHP1 in plant cells, despite the lack of sequence homology between UPAT and APOLO. In addition, we show that increased levels of APOLO or UPAT hamper VIM1 and LHP1 binding to YUCCA2 promoter and globally alter the Arabidopsis transcriptome in a similar manner. Conclusions: Collectively, our results uncover a new mechanism in which a plant lncRNA coordinates Polycomb action and DNA methylation through the interaction with VIM1, and indicates that evolutionary unrelated lncRNAs with potentially conserved structures may exert similar functions by interacting with homolog partners.
MeSH term(s)	Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; CCAAT-Enhancer-Binding Proteins/genetics ; CCAAT-Enhancer-Binding Proteins/metabolism ; DNA/metabolism ; DNA Methylation ; Histones/metabolism ; Humans ; Indoleacetic Acids/metabolism ; Plants/genetics ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism
Chemical Substances	Arabidopsis Proteins ; CCAAT-Enhancer-Binding Proteins ; Histones ; Indoleacetic Acids ; RNA, Long Noncoding ; DNA (9007-49-2) ; UHRF1 protein, human (EC 2.3.2.27) ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
Language	English
Publishing date	2022-08-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2040529-7
ISSN	1474-760X ; 1474-760X
ISSN (online)	1474-760X
ISSN	1474-760X
DOI	10.1186/s13059-022-02750-7
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Article ; Online: The Arabidopsis APOLO and human UPAT sequence-unrelated long noncoding RNAs can modulate DNA and histone methylation machineries in plants

Camille Fonouni-Farde / Aurélie Christ / Thomas Blein / María Florencia Legascue / Lucía Ferrero / Michaël Moison / Leandro Lucero / Juan Sebastián Ramírez-Prado / David Latrasse / Daniel Gonzalez / Moussa Benhamed / Leandro Quadrana / Martin Crespi / Federico Ariel

Genome Biology, Vol 23, Iss 1, Pp 1-

2022 Volume 30

Abstract: Abstract Background RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene ... ...

Abstract	Abstract Background RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene transcriptional activity. Results Here, we show that in addition to the PRC1-component LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), APOLO interacts with the methylcytosine-binding protein VARIANT IN METHYLATION 1 (VIM1), a conserved homolog of the mammalian DNA methylation regulator UBIQUITIN-LIKE CONTAINING PHD AND RING FINGER DOMAINS 1 (UHRF1). The APOLO-VIM1-LHP1 complex directly regulates the transcription of the auxin biosynthesis gene YUCCA2 by dynamically determining DNA methylation and H3K27me3 deposition over its promoter during the plant thermomorphogenic response. Strikingly, we demonstrate that the lncRNA UHRF1 Protein Associated Transcript (UPAT), a direct interactor of UHRF1 in humans, can be recognized by VIM1 and LHP1 in plant cells, despite the lack of sequence homology between UPAT and APOLO. In addition, we show that increased levels of APOLO or UPAT hamper VIM1 and LHP1 binding to YUCCA2 promoter and globally alter the Arabidopsis transcriptome in a similar manner. Conclusions Collectively, our results uncover a new mechanism in which a plant lncRNA coordinates Polycomb action and DNA methylation through the interaction with VIM1, and indicates that evolutionary unrelated lncRNAs with potentially conserved structures may exert similar functions by interacting with homolog partners.
Keywords	Long noncoding RNA ; DNA methylation ; PRC1 ; Polycomb ; RdDM ; Thermomorphogenesis ; Biology (General) ; QH301-705.5 ; Genetics ; QH426-470
Subject code	570
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The Arabidopsis APOLO and human UPAT sequence-unrelated long noncoding RNAs can modulate DNA and histone methylation machineries in plants

Genome biology. 2022 Dec., v. 23, no. 1

2022

Abstract: BACKGROUND: RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene ... ...

Abstract	BACKGROUND: RNA-DNA hybrid (R-loop)-associated long noncoding RNAs (lncRNAs), including the Arabidopsis lncRNA AUXIN-REGULATED PROMOTER LOOP (APOLO), are emerging as important regulators of three-dimensional chromatin conformation and gene transcriptional activity. RESULTS: Here, we show that in addition to the PRC1-component LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), APOLO interacts with the methylcytosine-binding protein VARIANT IN METHYLATION 1 (VIM1), a conserved homolog of the mammalian DNA methylation regulator UBIQUITIN-LIKE CONTAINING PHD AND RING FINGER DOMAINS 1 (UHRF1). The APOLO-VIM1-LHP1 complex directly regulates the transcription of the auxin biosynthesis gene YUCCA2 by dynamically determining DNA methylation and H3K27me3 deposition over its promoter during the plant thermomorphogenic response. Strikingly, we demonstrate that the lncRNA UHRF1 Protein Associated Transcript (UPAT), a direct interactor of UHRF1 in humans, can be recognized by VIM1 and LHP1 in plant cells, despite the lack of sequence homology between UPAT and APOLO. In addition, we show that increased levels of APOLO or UPAT hamper VIM1 and LHP1 binding to YUCCA2 promoter and globally alter the Arabidopsis transcriptome in a similar manner. CONCLUSIONS: Collectively, our results uncover a new mechanism in which a plant lncRNA coordinates Polycomb action and DNA methylation through the interaction with VIM1, and indicates that evolutionary unrelated lncRNAs with potentially conserved structures may exert similar functions by interacting with homolog partners.
Keywords	Arabidopsis ; DNA ; DNA methylation ; RING finger domains ; auxins ; biosynthesis ; genes ; heterochromatin ; histones ; humans ; hybrids ; sequence homology ; transcription (genetics) ; transcriptome
Language	English
Dates of publication	2022-12
Size	p. 181.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2040529-7
ISSN	1474-760X
ISSN	1474-760X
DOI	10.1186/s13059-022-02750-7
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Article ; Online: The matrix revolutions: towards the decoding of the plant chromatin three-dimensional reality.

Huang, Ying / Rodriguez-Granados, Natalia Yaneth / Latrasse, David / Raynaud, Cecile / Benhamed, Moussa / Ramirez-Prado, Juan Sebastian

Journal of experimental botany

2020 Volume 71, Issue 17, Page(s) 5129–5147

Abstract: In recent years, we have witnessed a significant increase in studies addressing the three-dimensional (3D) chromatin organization of the plant nucleus. Important advances in chromatin conformation capture (3C)-derived and related techniques have allowed ... ...

Abstract	In recent years, we have witnessed a significant increase in studies addressing the three-dimensional (3D) chromatin organization of the plant nucleus. Important advances in chromatin conformation capture (3C)-derived and related techniques have allowed the exploration of the nuclear topology of plants with large and complex genomes, including various crops. In addition, the increase in their resolution has permitted the depiction of chromatin compartmentalization and interactions at the gene scale. These studies have revealed the highly complex mechanisms governing plant nuclear architecture and the remarkable knowledge gaps in this field. Here we discuss the state-of-the-art in plant chromosome architecture, including our knowledge of the hierarchical organization of the genome in 3D space and regarding other nuclear components. Furthermore, we highlight the existence in plants of topologically associated domain (TAD)-like structures that display striking differences from their mammalian counterparts, proposing the concept of ICONS-intergenic condensed spacers. Similarly, we explore recent advances in the study of chromatin loops and R-loops, and their implication in the regulation of gene activity. Finally, we address the impact that polyploidization has had on the chromatin topology of modern crops, and how this is related to phenomena such as subgenome dominance and biased gene retention in these organisms.
MeSH term(s)	Animals ; Cell Nucleus/genetics ; Chromatin/genetics ; Chromosomes, Plant ; Genome ; Plants/genetics
Chemical Substances	Chromatin
Language	English
Publishing date	2020-07-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
DOI	10.1093/jxb/eraa322
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Article ; Online: Chromatin architecture: A new dimension in the dynamic control of gene expression.

Ramirez-Prado, Juan Sebastian / Rodriguez-Granados, Natalia Yaneth / Ariel, Federico / Raynaud, Cécile / Benhamed, Moussa

Plant signaling & behavior

2016 Volume 11, Issue 10, Page(s) e1232224

Abstract: As the most recent evidence of eukaryotic cell complexity, genome architecture has astounded the scientific community and prompted a variety of technical and cognitive challenges. Several technologies have emerged and evidenced the integration of ... ...

Abstract	As the most recent evidence of eukaryotic cell complexity, genome architecture has astounded the scientific community and prompted a variety of technical and cognitive challenges. Several technologies have emerged and evidenced the integration of chromatin packaging and topology, epigenetic processes, and transcription for the pertinent regulation of gene expression. In the present addendum we present and discuss some of our recent research, directed toward the holistic comprehension of the processes by which plants respond to environmental and developmental stimuli. We propose that the study of genome topology and genomic interactions is essential for the understanding of the molecular mechanisms behind a phenotype. Even though our knowledge and understanding of genome architecture and hierarchy has improved substantially in the last few years -in Arabidopsis and other eukaryotes -, there is still a long way ahead in this relatively new field of study. For this, it is necessary to take advantage of the high resolution of the emerging available techniques, and perform integrative approaches with which it will be possible to depict the role of chromatin architecture in the regulation of transcription and ultimately, physiological processes.
MeSH term(s)	Arabidopsis/genetics ; Arabidopsis/metabolism ; Chromatin/genetics ; Chromatin/metabolism ; Gene Expression/genetics ; RNA, Long Noncoding/genetics
Chemical Substances	Chromatin ; RNA, Long Noncoding
Language	English
Publishing date	2016-10-02
Publishing country	United States
Document type	Journal Article
ISSN	1559-2324
ISSN (online)	1559-2324
DOI	10.1080/15592324.2016.1232224
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Article ; Online: CmLHP1 proteins play a key role in plant development and sex determination in melon (Cucumis melo).

Rodriguez-Granados, Natalia Yaneth / Ramirez-Prado, Juan Sebastian / Brik-Chaouche, Rim / An, Jing / Manza-Mianza, Deborah / Sircar, Sanchari / Troadec, Christelle / Hanique, Melissa / Soulard, Camille / Costa, Rafael / Dogimont, Catherine / Latrasse, David / Raynaud, Cécile / Boualem, Adnane / Benhamed, Moussa / Bendahmane, Abdelhafid

The Plant journal : for cell and molecular biology

2021 Volume 109, Issue 5, Page(s) 1213–1228

Abstract: In monoecious melon (Cucumis melo), sex is determined by the differential expression of sex determination genes (SDGs) and adoption of sex-specific transcriptional programs. Histone modifications such as H3K27me3 have been previously shown to be a ... ...

Abstract	In monoecious melon (Cucumis melo), sex is determined by the differential expression of sex determination genes (SDGs) and adoption of sex-specific transcriptional programs. Histone modifications such as H3K27me3 have been previously shown to be a hallmark associated to unisexual flower development in melon; yet, no genetic approaches have been conducted for elucidating the roles of H3K27me3 writers, readers, and erasers in this process. Here we show that melon homologs to Arabidopsis LHP1, CmLHP1A and B, redundantly control several aspects of plant development, including sex expression. Cmlhp1ab double mutants displayed an overall loss and redistribution of H3K27me3, leading to a deregulation of genes involved in hormone responses, plant architecture, and flower development. Consequently, double mutants display pleiotropic phenotypes and, interestingly, a general increase of the male:female ratio. We associated this phenomenon with a general deregulation of some hormonal response genes and a local activation of male-promoting SDGs and MADS-box transcription factors. Altogether, these results reveal a novel function for CmLHP1 proteins in maintenance of monoecy and provide novel insights into the polycomb-mediated epigenomic regulation of sex lability in plants.
MeSH term(s)	Arabidopsis/genetics ; Cucumis melo/genetics ; Cucumis melo/metabolism ; Cucurbitaceae/genetics ; Gene Expression Regulation, Plant/genetics ; Histones/metabolism ; Plant Development ; Plant Proteins/genetics ; Plant Proteins/metabolism
Chemical Substances	Histones ; Plant Proteins
Language	English
Publishing date	2021-12-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1111/tpj.15627
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The Polycomb protein LHP1 regulates Arabidopsis thaliana stress responses through the repression of the MYC2-dependent branch of immunity.

Ramirez-Prado, Juan Sebastian / Latrasse, David / Rodriguez-Granados, Natalia Yaneth / Huang, Ying / Manza-Mianza, Deborah / Brik-Chaouche, Rim / Jaouannet, Maelle / Citerne, Sylvie / Bendahmane, Abdelhafid / Hirt, Heribert / Raynaud, Cecile / Benhamed, Moussa

The Plant journal : for cell and molecular biology

2019 Volume 100, Issue 6, Page(s) 1118–1131

Abstract: Polycomb repressive complexes (PRCs) have been traditionally associated with the regulation of developmental processes in various organisms, including higher plants. However, similar to other epigenetic regulators, there is accumulating evidence for ... ...

Abstract	Polycomb repressive complexes (PRCs) have been traditionally associated with the regulation of developmental processes in various organisms, including higher plants. However, similar to other epigenetic regulators, there is accumulating evidence for their role in the regulation of stress and immune-related pathways. In the current study we show that the PRC1 protein LHP1 is required for the repression of the MYC2 branch of jasmonic acid (JA)/ethylene (ET) pathway of immunity. Loss of LHP1 induces the reduction in H3K27me3 levels in the gene bodies of ANAC019 and ANAC055, as well as some of their targets, leading to their transcriptional upregulation. Consistently, increased expression of these two transcription factors leads to the misregulation of several of their genomic targets. The lhp1 mutant mimics the MYC2, ANAC019, and ANAC055 overexpressers in several of their phenotypes, including increased aphid resistance, abscisic acid (ABA) sensitivity and drought tolerance. In addition, like the MYC2 and ANAC overexpressers, lhp1 displays reduced salicylic acid (SA) content caused by a deregulation of ICS1 and BSMT1, as well as increased susceptibility to the hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000. Together, our results indicate that LHP1 regulates the expression of stress-responsive genes as well as the homeostasis and responses to the stress hormones SA and ABA. This protein emerges as a key chromatin player fine tuning the complex balance between developmental and stress-responsive processes.
MeSH term(s)	Abscisic Acid/metabolism ; Animals ; Aphids ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/metabolism ; Cyclopentanes ; Ethylenes/metabolism ; Gene Expression Regulation, Plant ; Oxylipins ; Plant Diseases/immunology ; Plant Immunity/immunology ; Plant Immunity/physiology ; Polycomb-Group Proteins ; Pseudomonas syringae/metabolism ; Pseudomonas syringae/pathogenicity ; Salicylic Acid/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcriptome
Chemical Substances	ANAC019 protein, Arabidopsis ; ANAC055 protein, Arabidopsis ; Arabidopsis Proteins ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; Cyclopentanes ; Ethylenes ; LHP1 protein, Arabidopsis ; MYC2 protein, Arabidopsis ; Oxylipins ; Polycomb-Group Proteins ; Transcription Factors ; jasmonic acid (6RI5N05OWW) ; Abscisic Acid (72S9A8J5GW) ; ethylene (91GW059KN7) ; Salicylic Acid (O414PZ4LPZ)
Language	English
Publishing date	2019-10-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1111/tpj.14502
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A new role for histone demethylases in the maintenance of plant genome integrity.

Antunez-Sanchez, Javier / Naish, Matthew / Ramirez-Prado, Juan Sebastian / Ohno, Sho / Huang, Ying / Dawson, Alexander / Opassathian, Korawit / Manza-Mianza, Deborah / Ariel, Federico / Raynaud, Cecile / Wibowo, Anjar / Daron, Josquin / Ueda, Minako / Latrasse, David / Slotkin, R Keith / Weigel, Detlef / Benhamed, Moussa / Gutierrez-Marcos, Jose

eLife

2020 Volume 9

Abstract: Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in the control of growth, development, and adaptation to environmental fluctuations of most multicellular eukaryotes. The catalytic activity of PRC2 is ... ...

Abstract	Histone modifications deposited by the Polycomb repressive complex 2 (PRC2) play a critical role in the control of growth, development, and adaptation to environmental fluctuations of most multicellular eukaryotes. The catalytic activity of PRC2 is counteracted by Jumonji-type (JMJ) histone demethylases, which shapes the genomic distribution of H3K27me3. Here, we show that two JMJ histone demethylases in
MeSH term(s)	Arabidopsis/enzymology ; Arabidopsis/genetics ; Arabidopsis/growth & development ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Gene Expression Regulation, Plant ; Genome, Plant ; Histones/metabolism ; Jumonji Domain-Containing Histone Demethylases/genetics ; Jumonji Domain-Containing Histone Demethylases/metabolism ; Polycomb Repressive Complex 2/genetics ; Polycomb Repressive Complex 2/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	Arabidopsis Proteins ; ELF6 protein, Arabidopsis ; Histones ; REF6 protein, Arabidopsis ; Transcription Factors ; Jumonji Domain-Containing Histone Demethylases (EC 1.14.11.-) ; Polycomb Repressive Complex 2 (EC 2.1.1.43)
Language	English
Publishing date	2020-10-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.58533
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Article: The Polycomb protein LHP1 regulates Arabidopsis thaliana stress responses through the repression of the MYC2‐dependent branch of immunity

Ramirez‐Prado, Juan Sebastian / Latrasse, David / Rodriguez‐Granados, Natalia Yaneth / Huang, Ying / Manza‐Mianza, Deborah / Brik‐Chaouche, Rim / Jaouannet, Maelle / Citerne, Sylvie / Bendahmane, Abdelhafid / Hirt, Heribert / Raynaud, Cecile / Benhamed, Moussa

plant journal. 2019 Dec., v. 100, no. 6

2019

Abstract	Polycomb repressive complexes (PRCs) have been traditionally associated with the regulation of developmental processes in various organisms, including higher plants. However, similar to other epigenetic regulators, there is accumulating evidence for their role in the regulation of stress and immune‐related pathways. In the current study we show that the PRC1 protein LHP1 is required for the repression of the MYC2 branch of jasmonic acid (JA)/ethylene (ET) pathway of immunity. Loss of LHP1 induces the reduction in H3K27me3 levels in the gene bodies of ANAC019 and ANAC055, as well as some of their targets, leading to their transcriptional upregulation. Consistently, increased expression of these two transcription factors leads to the misregulation of several of their genomic targets. The lhp1 mutant mimics the MYC2, ANAC019, and ANAC055 overexpressers in several of their phenotypes, including increased aphid resistance, abscisic acid (ABA) sensitivity and drought tolerance. In addition, like the MYC2 and ANAC overexpressers, lhp1 displays reduced salicylic acid (SA) content caused by a deregulation of ICS1 and BSMT1, as well as increased susceptibility to the hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000. Together, our results indicate that LHP1 regulates the expression of stress‐responsive genes as well as the homeostasis and responses to the stress hormones SA and ABA. This protein emerges as a key chromatin player fine tuning the complex balance between developmental and stress‐responsive processes.
Keywords	Aphidoidea ; Arabidopsis thaliana ; Pseudomonas syringae pv. tomato ; abscisic acid ; chromatin ; disease susceptibility ; drought tolerance ; epigenetics ; ethylene ; gene expression regulation ; gene overexpression ; genes ; genomics ; homeostasis ; hormones ; immunity ; jasmonic acid ; mutants ; pathogens ; phenotype ; salicylic acid ; stress response ; transcription (genetics) ; transcription factors
Language	English
Dates of publication	2019-12
Size	p. 1118-1131.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1111/tpj.14502
Database	NAL-Catalogue (AGRICOLA)

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Article: CmLHP1 proteins play a key role in plant development and sex determination in melon (Cucumis melo)

Rodriguez‐Granados, Natalia Yaneth / Ramirez‐Prado, Juan Sebastian / Brik‐Chaouche, Rim / An, Jing / Manza‐Mianza, Deborah / Sircar, Sanchari / Troadec, Christelle / Hanique, Melissa / Soulard, Camille / Costa, Rafael / Dogimont, Catherine / Latrasse, David / Raynaud, Cécile / Boualem, Adnane / Benhamed, Moussa / Bendahmane, Abdelhafid

plant journal. 2022 Mar., v. 109, no. 5

2022

Abstract: In monoecious melon (Cucumis melo), sex is determined by the differential expression of sex determination genes (SDGs) and adoption of sex‐specific transcriptional programs. Histone modifications such as H3K27me3 have been previously shown to be a ... ...

Abstract	In monoecious melon (Cucumis melo), sex is determined by the differential expression of sex determination genes (SDGs) and adoption of sex‐specific transcriptional programs. Histone modifications such as H3K27me3 have been previously shown to be a hallmark associated to unisexual flower development in melon; yet, no genetic approaches have been conducted for elucidating the roles of H3K27me3 writers, readers, and erasers in this process. Here we show that melon homologs to Arabidopsis LHP1, CmLHP1A and B, redundantly control several aspects of plant development, including sex expression. Cmlhp1ab double mutants displayed an overall loss and redistribution of H3K27me3, leading to a deregulation of genes involved in hormone responses, plant architecture, and flower development. Consequently, double mutants display pleiotropic phenotypes and, interestingly, a general increase of the male:female ratio. We associated this phenomenon with a general deregulation of some hormonal response genes and a local activation of male‐promoting SDGs and MADS‐box transcription factors. Altogether, these results reveal a novel function for CmLHP1 proteins in maintenance of monoecy and provide novel insights into the polycomb‐mediated epigenomic regulation of sex lability in plants.
Keywords	Arabidopsis ; Cucumis melo ; epigenome ; flowering ; gene expression regulation ; histones ; melons ; monoecy ; plant architecture ; sex determination ; transcription (genetics)
Language	English
Dates of publication	2022-03
Size	p. 1213-1228.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1111/tpj.15627
Database	NAL-Catalogue (AGRICOLA)

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