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Article ; Online: Mass Cytometry in Food Allergy Research.

Acevedo, Nathalie / Llinás-Caballero, Kevin

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2717, Page(s) 207–219

Abstract: Mechanisms underlying food allergy are not well understood. Mass cytometry is a technique that allows the multiple analysis of cell surface markers and intracellular proteins by using the spectrum of rare metal isotopes of different atomic masses without ...

Abstract	Mechanisms underlying food allergy are not well understood. Mass cytometry is a technique that allows the multiple analysis of cell surface markers and intracellular proteins by using the spectrum of rare metal isotopes of different atomic masses without channel overlap. Bioinformatic approaches are implemented to combine and reduce the information of more than 60 parameters to define immune cell subpopulations. To date, mass cytometry has revealed a great heterogeneity in human response to food antigens and that subpopulations of basophils and mononuclear cells might be mechanistically implicated in food allergy. This chapter reviews some fundamentals of mass cytometry and the contributions of this technique in elucidating the immune basis of food allergy, oral tolerance, food desensitization, phenotypes, and the cellular events occurring upon allergen-specific immunotherapy.
MeSH term(s)	Humans ; Food Hypersensitivity ; Food ; Basophils ; Computational Biology ; Desensitization, Immunologic
Language	English
Publishing date	2023-09-22
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3453-0_13
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Article ; Online: Inmunofenotipo por citometría espectral, revela un perfil de linfopenia asociado a desregulación con aumento de linfocitos de memoria efectora en paciente con mutación en el gen ITPR3.

Muñoz, César / de Vivero, María Mónica / Acevedo, Nathalie

Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)

2024 Volume 71, Issue 1, Page(s) 59

Abstract: Background: Variants in intracellular calcium transport genes have been associated with syndromic immunodeficiencies with a SCID phenotype.: Case report: Seven-year-old girl of non-consanguineous parents, in Cartagena-Colombia. At two months of age, ... ...

Title translation	Immunophenotyping by spectral cytometry reveals a profile of lymphopenia associated with deregulation with an increase in effector memory lymphocytes in a patient with a mutation in the ITPR3 gene.
Abstract	Background: Variants in intracellular calcium transport genes have been associated with syndromic immunodeficiencies with a SCID phenotype. Case report: Seven-year-old girl of non-consanguineous parents, in Cartagena-Colombia. At two months of age, he presented hematochezia and was diagnosed with alimentary proctolitis without improvement with restriction to milk, wheat and eggs, and malnutrition developed. At eight months, a colon biopsy shows chronic lymphoid hyperplasia, presenting with anemia, eosinophilia, but total and specific IgE to normal foods. After four years, the Immunology Service found her asymptomatic, nutritionally recovered and without allergic sensitization, but eosinophilia and elevated calprotectin persisted, suggesting an early-onset inflammatory bowel disease. Immunoglobulins were normal, lymphocyte populations with CD3, CD4 and CD8 lymphopenia. At six years old, she presented atopic dermatitis, still had elevated calprotectin and was lymphopenic. Immunophenotyping by spectral cytometry using Cytek Conclusions: The clinical features and immunophenotype of this candidate variant differ from others related to intracellular calcium transport. They are functional studies necessary to validate their causality. A patient with a potentially deleted variant presents an immunophenotype with CD3 lymphopenia and persistent lymphocyte activation.
MeSH term(s)	Humans ; Female ; Child ; Lymphopenia/genetics ; Lymphopenia/etiology ; Immunophenotyping ; Inositol 1,4,5-Trisphosphate Receptors/genetics ; Mutation ; Flow Cytometry ; Memory T Cells/immunology
Chemical Substances	Inositol 1,4,5-Trisphosphate Receptors ; ITPR3 protein, human
Language	Spanish
Publishing date	2024-02-01
Publishing country	Mexico
Document type	Case Reports ; Journal Article ; English Abstract
ZDB-ID	639125-4
ISSN	2448-9190 ; 0002-5151
ISSN (online)	2448-9190
ISSN	0002-5151
DOI	10.29262/ram.v71i1.1361
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Article ; Online: Are the Terms Major and Minor Allergens Useful for Precision Allergology?

Caraballo, Luis / Valenta, Rudolf / Acevedo, Nathalie / Zakzuk, Josefina

Frontiers in immunology

2021 Volume 12, Page(s) 651500

MeSH term(s)	Allergens/adverse effects ; Allergens/metabolism ; Allergy and Immunology ; Animals ; Asthma/blood ; Asthma/genetics ; Asthma/immunology ; Asthma/therapy ; Dermatophagoides pteronyssinus/immunology ; Humans ; Immunoglobulin E/blood ; Immunoglobulin E/metabolism ; Precision Medicine/methods ; Protein Binding/immunology ; Rhinitis, Allergic/blood ; Rhinitis, Allergic/genetics ; Rhinitis, Allergic/immunology ; Rhinitis, Allergic/therapy ; Terminology as Topic
Chemical Substances	Allergens ; Immunoglobulin E (37341-29-0)
Language	English
Publishing date	2021-03-08
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.651500
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Article ; Online: Understanding the Cellular Sources of the Fractional Exhaled Nitric Oxide (FeNO) and Its Role as a Biomarker of Type 2 Inflammation in Asthma.

Escamilla-Gil, Jose M / Fernandez-Nieto, Mar / Acevedo, Nathalie

BioMed research international

2022 Volume 2022, Page(s) 5753524

Abstract: Fractional exhaled nitric oxide (FeNO) has gained great clinical importance as a biomarker of type 2 inflammation in chronic airway diseases such as asthma. FeNO originates primarily in the bronchial epithelium and is produced in large quantities by the ... ...

Abstract	Fractional exhaled nitric oxide (FeNO) has gained great clinical importance as a biomarker of type 2 inflammation in chronic airway diseases such as asthma. FeNO originates primarily in the bronchial epithelium and is produced in large quantities by the enzyme inducible nitric oxide synthase (iNOS). It should be noted that nitric oxide (NO) produced at femtomolar to picomolar levels is fundamental for respiratory physiology. This basal production is induced in the bronchial epithelium by interferon gamma (IFN
MeSH term(s)	Asthma/drug therapy ; Biomarkers/metabolism ; Breath Tests ; Exhalation ; Fractional Exhaled Nitric Oxide Testing ; Humans ; Inflammation ; Nitric Oxide/metabolism
Chemical Substances	Biomarkers ; Nitric Oxide (31C4KY9ESH)
Language	English
Publishing date	2022-05-02
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2022/5753524
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Article ; Online: Cystatin from the helminth

Acevedo, Nathalie / Lozano, Ana / Zakzuk, Josefina / Llinás-Caballero, Kevin / Brodin, David / Nejsum, Peter / Williams, Andrew R / Caraballo, Luis

Frontiers in immunology

2024 Volume 15, Page(s) 1328401

Abstract: Background: Ascaris lumbricoides: Methods: moDC were generated from peripheral blood monocytes from six healthy human donors of Denmark, stimulated with 1 µM of Al-CPI, and cultured for 5 hours at 37°C. RNA was sequenced using TrueSeq RNA libraries ... ...

Abstract	Background: Ascaris lumbricoides Methods: moDC were generated from peripheral blood monocytes from six healthy human donors of Denmark, stimulated with 1 µM of Al-CPI, and cultured for 5 hours at 37°C. RNA was sequenced using TrueSeq RNA libraries and the NextSeq 550 v2.5 (75 cycles) sequencing kit (Illumina, Inc). After QC, reads were aligned to the human GRCh38 genome using Spliced Transcripts Alignment to a Reference (STAR) software. Differential expression was calculated by DESEq2 and expressed in fold changes (FC). Cell surface markers and cytokine production by moDC were evaluated by flow cytometry. Results: Compared to unstimulated cells, Al-CPI stimulated moDC showed differential expression of 444 transcripts (\|FC\| ≥1.3). The top significant differences were in Kruppel-like factor 10 ( Conclusion: Al-CPI modifies the transcriptome of moDC, increasing several transcripts encoding enzymes involved in cholesterol biosynthesis and SREBP signaling. Moreover, Al-CPI target several transcripts in the TNF-alpha signaling pathway influencing cytokine release by moDC. In addition, mRNA levels of genes encoding
MeSH term(s)	Humans ; Animals ; Mice ; Monocytes ; Ascaris lumbricoides ; Mevalonic Acid/metabolism ; Sterol Regulatory Element Binding Protein 1/metabolism ; Cell Differentiation ; Cytokines/metabolism ; Cystatins ; Inflammation/metabolism ; Immunity ; Dendritic Cells ; RNA/metabolism
Chemical Substances	Mevalonic Acid (S5UOB36OCZ) ; Sterol Regulatory Element Binding Protein 1 ; Cytokines ; Cystatins ; RNA (63231-63-0)
Language	English
Publishing date	2024-02-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1328401
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Article ; Online: Caracterización de fenotipos de asma en niños del trópico.

Guzmán, María Camila / de Vivero, María / Gil, Isabel / Marrugo, Victoria / Mercado, Dilia / Zakzuk, Josefina / Puerta, Leonardo / Acevedo, Nathalie / Caraballo, Luis

Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)

2024 Volume 71, Issue 1, Page(s) 74

Abstract: Objective: Determine the main asthma phenotypes in a population of asthmatic children in Cartagena, Colombia.: Methods: 107 children (7 to 17 years old) with a previous diagnosis of asthma were recruited. Biomarkers of T2 inflammation were evaluated ... ...

Title translation	Characterization of asthma phenotypes in children of the tropics.
Abstract	Objective: Determine the main asthma phenotypes in a population of asthmatic children in Cartagena, Colombia. Methods: 107 children (7 to 17 years old) with a previous diagnosis of asthma were recruited. Biomarkers of T2 inflammation were evaluated by measuring FeNO, eosinophil count in peripheral blood by hemocytometry, and determination of specific IgE to mite allergens by ELISA. The study was approved by the ethics committee of the University of Cartagena (SGR, Grant BPIN2020000100405). Results: The average age of patients was 10,9 years. 19,6% of the children did not show elevation of any of the T2 inflammation biomarkers evaluated (FeNO<20ppb, eos<300/ul, negative specific IgE), so they were considered patients with non-allergic asthma (non-T2). 71,9% of all patients were sensitized to at least one allergen, this phenotype was considered allergic asthma. 30,8% of the patients presented the three elevated biomarkers (FeNO>20ppb + eos >300/ul + positive specific IgE), this phenotype was classified as high T2 allergic asthma. A moderate correlation (Spearman rho=0,44, p<0,0001) was found between FeNO values and eosinophil counts. Conclusion: In this study, the following phenotypes were found: allergic asthma, high T2 asthma, and non-allergic asthma. Most patients presented a type 2 inflammatory phenotype with allergic sensitization. In addition to the measurement of specific IgE, the use of FeNO and eosinophil count in peripheral blood help to accurately determine those patients with high T2 asthma phenotypes.
MeSH term(s)	Humans ; Asthma/blood ; Child ; Phenotype ; Adolescent ; Male ; Female ; Immunoglobulin E/blood ; Eosinophils ; Tropical Climate ; Biomarkers/blood ; Colombia ; Leukocyte Count
Chemical Substances	Immunoglobulin E (37341-29-0) ; Biomarkers
Language	Spanish
Publishing date	2024-02-01
Publishing country	Mexico
Document type	English Abstract ; Journal Article
ZDB-ID	639125-4
ISSN	2448-9190 ; 0002-5151
ISSN (online)	2448-9190
ISSN	0002-5151
DOI	10.29262/ram.v71i1.1362
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Article ; Online: Caracterización de poblaciones celulares en sangre periférica, con relación a la infección por

Lozano, Ana / Marrugo, Victoria / Alvarado, Juan Carlos / Hernandez, Karen / Caballero, Kevin Llinás / Acevedo, Nathalie / Zakzuk, Josefina / Caraballo, Luis

Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)

2024 Volume 71, Issue 1, Page(s) 69

Abstract: Objective: To compare the relative frequencies of immune cell populations in the peripheral blood according to : Methods: Peripheral blood samples were collected from participants infected (n = 35) and uninfected with : Results: No significant ... ...

Title translation	Characterization of peripheral blood cellular populations in relation to Ascaris Lumbricoides infection in rural areas of Bolívar.
Abstract	Objective: To compare the relative frequencies of immune cell populations in the peripheral blood according to Methods: Peripheral blood samples were collected from participants infected (n = 35) and uninfected with Results: No significant differences were observed in age [mean cases: 35.69 (SD: 17.7) vs. controls: 37.04 (SD: 15.6) years] or sex (cases: 62.9% vs. controls: 74.1%) (Table 1). All infections were mild, with a median of 96 eggs (IQR, 48-216). A marginally significant difference was observed only in the percentage of neutrophils (45.37% in cases vs. 54.79% in controls, p=0.041) (Figure 3). Although the frequency of eosinophils was higher in the cases (8.1% vs. 6%), this difference was not significant (p=0.138) (Figure 3). No significant differences were observed in the populations of monocytes or dendritic cells between cases and controls (Figure 4). Conclusion: Mild
MeSH term(s)	Humans ; Male ; Female ; Ascariasis/immunology ; Ascariasis/epidemiology ; Adult ; Adolescent ; Animals ; Young Adult ; Rural Health ; Child ; Ascaris lumbricoides ; Middle Aged ; Colombia
Language	Spanish
Publishing date	2024-02-01
Publishing country	Mexico
Document type	English Abstract ; Journal Article
ZDB-ID	639125-4
ISSN	2448-9190 ; 0002-5151
ISSN (online)	2448-9190
ISSN	0002-5151
DOI	10.29262/ram.v71i1.1372
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Article ; Online: Determinación de poblaciones circulantes en sangre periférica de linfocitos T, reguladores CD4

Barrios-Angulo, Carlos E / de Vivero, María Mónica / Reina-Rivero, Randy / Guzmán, María Camila / Caballero, Miguel Ángel / Acevedo, Nathalie

Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)

2024 Volume 71, Issue 1, Page(s) 73

Abstract: Objective: To carry out a preliminary analysis on the Treg lymphocyte counts present in the peripheral blood of allergic asthmatic children from the city of Cartagena, Colombia, compared to healthy controls.: Methods: We compared cytometry counts of ... ...

Title translation	Circulating populations of CD4+ CD25+ CD127- regulatory t lymphocytes in peripheral blood of allergic asthmatic children.
Abstract	Objective: To carry out a preliminary analysis on the Treg lymphocyte counts present in the peripheral blood of allergic asthmatic children from the city of Cartagena, Colombia, compared to healthy controls. Methods: We compared cytometry counts of ten asthmatic patients (age 7-16 years) and seven healthy controls (6-12 years), recruited in the city of Cartagena. Peripheral blood samples were stained using Cytek's 14-color cFluor Immunoprofiling kit (Cytek Results: The frequency of CD3+, CD4+, CD25+, CD127- Tregs was 11% of all CD4+ T cells, with a range of minimum 8,1% and maximum 17,7%. There was no significant difference in the proportion of Tregs between allergic asthmatic patients and healthy controls (P = 0,2). Conclusions: With this preliminary sample size, no significant differences were found in the Treg lymphocyte population between allergic asthmatic patients and healthy controls. The 14-color multiplexed panel is a useful tool not only to count CD3+ and CD4+ populations, but also to obtain the percentage of regulatory T cells using cell surface markers.
MeSH term(s)	Humans ; Child ; Asthma/blood ; Asthma/immunology ; T-Lymphocytes, Regulatory/immunology ; Adolescent ; Male ; Female ; Interleukin-2 Receptor alpha Subunit/blood ; Interleukin-2 Receptor alpha Subunit/analysis ; Interleukin-7 Receptor alpha Subunit/analysis ; Interleukin-7 Receptor alpha Subunit/blood ; CD4 Antigens/analysis ; CD4 Antigens/blood ; Lymphocyte Count
Chemical Substances	Interleukin-2 Receptor alpha Subunit ; Interleukin-7 Receptor alpha Subunit ; CD4 Antigens
Language	Spanish
Publishing date	2024-02-01
Publishing country	Mexico
Document type	English Abstract ; Journal Article
ZDB-ID	639125-4
ISSN	2448-9190 ; 0002-5151
ISSN (online)	2448-9190
ISSN	0002-5151
DOI	10.29262/ram.v71i1.1363
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Article ; Online: Medición de citoquinas mediante una técnica multiplex por citometría espectral: aplicación en el estudio de la inmunobiología de la ascariasis.

Lozano, Ana / Sánchez-Marrugo, Carolina / Llinás-Caballero, Kevin / Barrios, Carlos / Acevedo, Nathalie / Zakzuk, Josefina / Caraballo, Luis

Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993)

2024 Volume 71, Issue 1, Page(s) 64–65

Abstract: Objective: To quantify the production of Th1/Th2/Th17 cytokines induced by Ascaris lumbricoides antigens in peripheral blood mononuclear cells (PBMCs) using a multiplex technique.: Methods: PBMCs were cultured from individuals with mild A. ... ...

Title translation	Cytokine measurement using a multiplex technique with spectral flow cytometry: application in the study of the immunobiology of ascariasis.
Abstract	Objective: To quantify the production of Th1/Th2/Th17 cytokines induced by Ascaris lumbricoides antigens in peripheral blood mononuclear cells (PBMCs) using a multiplex technique. Methods: PBMCs were cultured from individuals with mild A. lumbricoides infection (n = 20) and uninfected individuals (n = 21) and stimulated with A. lumbricoides extract (ExtAscaris), a mix of anti-CD2/CD3/CD28 (CDmix) as a positive control, and only medium (negative control). Cytokines in the supernatants were measured using the BD™ Cytometric Bead Array Human Th1/Th2/Th17 kit, to identify IFN-γ, TNF, IL-10, IL-6, IL-4, IL-2, and IL-17A. Readings were taken on a spectral cytometer (Northern Lights, Cytek, USA), and analysis was performed using R software with packages "tidyverse," "beadplexr," "flowCore," and "arsenal." Cytokine concentrations were calculated using a 5-parameter logistic curve. The t-test was used to compare cases and controls, and statistical significance was set at p < 0.05. The study was approved by the Ethics Committee of the University of Cartagena and the participants provided informed consent. This study was financially supported by the Colombian Sistema General de Regalías under the BPIN2020000100405 - BPIN2020000100364. Results: Efficient fluorescence intensity extraction for each cytokine was achieved using detection channel R8 and the "mclust" clustering model (Figure 1). No significant differences were found in the levels of the seven cytokines between cases and controls (Figure 2). Although the IFN-γ response to ExtAscaris was higher in cases than in controls (252.5 ng/mL vs. 173.1 ng/mL), the difference was not significant. IL-17A (detection limit: 18.9 pg/mL) was more detectable in cases than controls (5 cases, 23% vs. 2 controls, 9.5%). IL-4 was only detected in the supernatants from CDmix-stimulated cultures but not with the Ascaris extract (Figure 2). Conclusions: The multiplex technique using spectral flow cytometry combined with open-source
MeSH term(s)	Humans ; Cytokines/blood ; Flow Cytometry/methods ; Ascariasis/immunology ; Ascariasis/diagnosis ; Male ; Female ; Adult ; Animals ; Leukocytes, Mononuclear/immunology ; Ascaris lumbricoides/immunology ; Young Adult ; Middle Aged ; Adolescent ; Antigens, Helminth/immunology
Chemical Substances	Cytokines ; Antigens, Helminth
Language	Spanish
Publishing date	2024-02-01
Publishing country	Mexico
Document type	English Abstract ; Journal Article
ZDB-ID	639125-4
ISSN	2448-9190 ; 0002-5151
ISSN (online)	2448-9190
ISSN	0002-5151
DOI	10.29262/ram.v71i1.1365
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Article ; Online: Are the Terms Major and Minor Allergens Useful for Precision Allergology?

Luis Caraballo / Rudolf Valenta / Nathalie Acevedo / Josefina Zakzuk

Frontiers in Immunology, Vol

2021 Volume 12

Keywords	allergen ; major and minor ; immunoglobulin E ; asthma ; specific immunoglobulin E ; precision medicine ; Immunologic diseases. Allergy ; RC581-607
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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