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Article ; Online: Update meta-analysis from biomedical literature about MTHFR'polymorphisms and the CML' risk.

Turki, Fatma / Louati, Nour / Kamoun, Hassen / Keskes, Leila / Rebaii, Tarek / Frikha, Rim

2022 Volume 100, Issue 4, Page(s) 285–294

Abstract: Background: The MTHFR gene polymorphisms are closely related to the chronic myeloid leukemia (CML). Case-control studies have associated the MTHFR polymorphisms and susceptibility to CML but the results were not conclusive.: Aim: To assess this ... ...

Abstract	Background: The MTHFR gene polymorphisms are closely related to the chronic myeloid leukemia (CML). Case-control studies have associated the MTHFR polymorphisms and susceptibility to CML but the results were not conclusive. Aim: To assess this association through an update meta-analysis. Methods: A descriptive and qualitative study was conducted among students in the 6th year of the faculty during the academic year 2020/2021. The data were collected through a questionnaire written in french evaluating the teaching methods. A focus group of ten persons was led to understand better student's opinions. Results: Totally, 17 and 12 case-control studies including CML cases and controls were enrolled in the meta-analysis respectively for C677T and A1298C polymorphism and CML risk. A poor association between the C677T (T vs C OR= 1,28; IC95%= [1,01;1,63]; p=0,04) and the one not significant between the A1298C (C vs A OR= 1,52; IC95%= [0,92; 2,51]; p= 0,1) polymorphisms and the CML risk for overall population were found. Conclusion: The results of this meta-analysis suggested no significant association between C677T and A1298C polymorphisms and CML risk leading to consider other factors such us folic acid intake, gene-gene and gene- environment interactions.
MeSH term(s)	Case-Control Studies ; Folic Acid ; Genetic Predisposition to Disease ; Genotype ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/epidemiology ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics ; Methylenetetrahydrofolate Reductase (NADPH2)/genetics ; Polymorphism, Genetic ; Risk Factors
Chemical Substances	Folic Acid (935E97BOY8) ; MTHFR protein, human (EC 1.5.1.20) ; Methylenetetrahydrofolate Reductase (NADPH2) (EC 1.5.1.20)
Language	English
Publishing date	2022-09-22
Publishing country	Tunisia
Document type	Journal Article ; Meta-Analysis
ZDB-ID	128627-4
ISSN	2724-7031 ; 0041-4131
ISSN (online)	2724-7031
ISSN	0041-4131
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Article ; Online: Removal of Acid Orange 51 by micro zero-valent iron under different operational conditions and evaluation of toxicity.

Ghariani, Bouthaina / Messaoud, Mouna / Louati, Ibtihel / Mtibaà, Rim / Nasri, Moncef / Mechichi, Tahar

Environmental science and pollution research international

2019 Volume 26, Issue 18, Page(s) 18392–18402

Abstract: The removal of Acid Orange 51 (AO 51) dye in aqueous solution by microscale zero-valent iron (m-ZVI) was investigated. The m-ZVI powder was characterized granulometrically by laser particle sizer and morphologically by transmission electron microscopy ( ... ...

Abstract	The removal of Acid Orange 51 (AO 51) dye in aqueous solution by microscale zero-valent iron (m-ZVI) was investigated. The m-ZVI powder was characterized granulometrically by laser particle sizer and morphologically by transmission electron microscopy (TEM). The effects of pH, m-ZVI concentration, H
MeSH term(s)	Adsorption ; Azo Compounds/analysis ; Hydrogen Peroxide/chemistry ; Iron/chemistry ; Surface Properties ; Water Pollutants, Chemical/analysis ; Water Purification/methods
Chemical Substances	Azo Compounds ; Water Pollutants, Chemical ; acid orange 51 (8003-88-1) ; Hydrogen Peroxide (BBX060AN9V) ; Iron (E1UOL152H7)
Language	English
Publishing date	2019-05-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-019-04929-1
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Article ; Online: Research of Pesticide Metabolites in Human Brain Tumor Tissues by Chemometrics-Based Gas Chromatography-Mass Spectrometry Analysis for a Hypothetical Correlation between Pesticide Exposure and Risk Factor of Central Nervous System Tumors.

Louati, Kaouthar / Kolsi, Fatma / Kallel, Rim / Gdoura, Yassine / Borni, Mahdi / Hakim, Leila Sellami / Zribi, Rania / Choura, Sirine / Maalej, Amina / Sayadi, Sami / Chamkha, Mohamed / Mnif, Basma / Khemakhem, Zouheir / Boudawara, Tahya Sellami / Boudawara, Mohamed Zaher / Safta, Fathi

ACS omega

2023 Volume 8, Issue 32, Page(s) 29812–29835

Abstract: Pesticides are widely used, resulting in continuing human exposure with potential health impacts. Some exposures related to agricultural works have been associated with neurological disorders. Since the 2000s, the hypothesis of the role of pesticides in ... ...

Abstract	Pesticides are widely used, resulting in continuing human exposure with potential health impacts. Some exposures related to agricultural works have been associated with neurological disorders. Since the 2000s, the hypothesis of the role of pesticides in the occurrence of central nervous system (CNS) tumors has been better documented in the literature. However, the etiology of childhood brain cancers still remains largely unknown. The major objective of this work was to assess the potential role of pesticide exposure as a risk factor for CNS tumors based on questionnaires and statistical analysis of information collected from patients hospitalized in the Neurosurgery Department of the Habib Bourguiba Hospital Medium in Sfax, Tunisia, during the period from January 1, 2022, to May 31, 2023. It also aimed to develop a simple and rapid analytical method by the gas chromatography-mass spectrometry technique for the research traces of pesticide metabolites in some collected human brain tumor tissues in order to more emphasize our hypothesis for such a correlation between pesticide exposure and brain tumor development. Patients with a history of high-risk exposure were selected to conduct further analysis. Chemometric methods were adapted to discern intrinsic variation between pathological and control groups and ascertain effective separation with the identification of differentially expressed metabolites accountable for such variations. Three samples revealed traces of pesticide metabolites that were mostly detected at an early age. The histopathological diagnosis was medulloblastoma for a 10-year-old child and high-grade gliomas for 27- and 35-year-old adults. The bivariate analyses (odds ratio >1 and
Language	English
Publishing date	2023-08-03
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c04592
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Article ; Online: Differential Proteome Profiling Analysis under Pesticide Stress by the Use of a Nano-UHPLC-MS/MS Untargeted Proteomic-Based Approach on a 3D-Developed Neurospheroid Model: Identification of Protein Interactions, Prognostic Biomarkers, and Potential Therapeutic Targets in Human IDH Mutant High-Grade Gliomas.

Louati, Kaouthar / Maalej, Amina / Kolsi, Fatma / Kallel, Rim / Gdoura, Yassine / Borni, Mahdi / Hakim, Leila Sellami / Zribi, Rania / Choura, Sirine / Sayadi, Sami / Chamkha, Mohamed / Mnif, Basma / Khemakhem, Zouheir / Boudawara, Tahya Sellami / Boudawara, Mohamed Zaher / Safta, Fathi

Journal of proteome research

2023 Volume 22, Issue 11, Page(s) 3534–3558

Abstract: High-grade gliomas represent the most common group of infiltrative primary brain tumors in adults associated with high invasiveness, agressivity, and resistance to therapy, which highlights the need to develop potent drugs with novel mechanisms of action. ...

Abstract	High-grade gliomas represent the most common group of infiltrative primary brain tumors in adults associated with high invasiveness, agressivity, and resistance to therapy, which highlights the need to develop potent drugs with novel mechanisms of action. The aim of this study is to reveal changes in proteome profiles under stressful conditions to identify prognostic biomarkers and altered apoptogenic pathways involved in the anticancer action of human isocitrate dehydrogenase (IDH) mutant high-grade gliomas. Our protocol consists first of a 3D
MeSH term(s)	Adult ; Humans ; Isocitrate Dehydrogenase/genetics ; Proteome/genetics ; Brain Neoplasms/drug therapy ; Brain Neoplasms/genetics ; Brain Neoplasms/metabolism ; Tandem Mass Spectrometry ; Prognosis ; Proteomics/methods ; Mutation ; Glioma/drug therapy ; Glioma/genetics ; Glioma/metabolism ; Biomarkers
Chemical Substances	Isocitrate Dehydrogenase (EC 1.1.1.41) ; Proteome ; Biomarkers
Language	English
Publishing date	2023-08-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.3c00395
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Article ; Online: Shotgun Proteomic-Based Approach with a Q-Exactive Hybrid Quadrupole-Orbitrap High-Resolution Mass Spectrometer for Protein Adductomics on a 3D Human Brain Tumor Neurospheroid Culture Model: The Identification of Adduct Formation in Calmodulin-Dependent Protein Kinase-2 and Annexin-A1 Induced by Pesticide Mixture.

Journal of proteome research

2023 Volume 22, Issue 12, Page(s) 3811–3832

Abstract: Pesticides are increasingly used in combinations in crop protection, resulting in enhanced toxicities for various organisms. Although protein adductomics is challenging, it remains a powerful bioanalytical tool to check environmental exposure and ... ...

Abstract	Pesticides are increasingly used in combinations in crop protection, resulting in enhanced toxicities for various organisms. Although protein adductomics is challenging, it remains a powerful bioanalytical tool to check environmental exposure and characterize xenobiotic adducts as putative toxicity biomarkers with high accuracy, facilitated by recent advances in proteomic methodologies and a mass spectrometry high-throughput technique. The present study aims to predict the potential neurotoxicity effect of imidacloprid and λ-cyhalothrin insecticides on human neural cells. Our protocol consisted first of 3D in vitro developing neurospheroids derived from human brain tumors and then treatment by pesticide mixture. Furthermore, we adopted a bottom-up proteomic-based approach using nanoflow ultraperformance liquid chromatography coupled with a high-resolution mass spectrometer for protein-adduct analysis with prediction of altered sites. Two proteins were selected, namely, calcium-calmodulin-dependent protein kinase-II (CaMK2) and annexin-A1 (ANXA1), as key targets endowed with primordial roles.
MeSH term(s)	Humans ; Pesticides ; Proteomics/methods ; Annexin A1 ; Mass Spectrometry/methods ; Proteins ; Calcium-Calmodulin-Dependent Protein Kinases ; Brain Neoplasms
Chemical Substances	Pesticides ; Annexin A1 ; Proteins ; Calcium-Calmodulin-Dependent Protein Kinases (EC 2.7.11.17)
Language	English
Publishing date	2023-10-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.3c00484
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Article: Removal of Acid Orange 51 by micro zero-valent iron under different operational conditions and evaluation of toxicity

Ghariani, Bouthaina / Messaoud, Mouna / Louati, Ibtihel / Mtibaà, Rim / Nasri, Moncef / Mechichi, Tahar

Environmental science and pollution research international. 2019 June, v. 26, no. 18

2019

Abstract	The removal of Acid Orange 51 (AO 51) dye in aqueous solution by microscale zero-valent iron (m-ZVI) was investigated. The m-ZVI powder was characterized granulometrically by laser particle sizer and morphologically by transmission electron microscopy (TEM). The effects of pH, m-ZVI concentration, H2O2 addition, and dye concentration on the decolorization of AO 51 were experimentally investigated. Results indicate that the removal efficiency is independent from pH values, increases with increasing ZVI dosage, and decreases with dye concentration. With 1 g/L of m-ZVI, AO 51 was effectively removed without and with addition of 25 mM H2O2, yielding a decolorization efficiency of around 70% and 98%, respectively, at pH 3 within 60 min of reaction time. The involvement of ̇OH in oxidizing AO 51 was examined by measuring the removal rates based on ̇OH scavenging molecule. Finally, the disappearance of AO 51 was estimated by monitoring the UV–Vis spectral evolution after 120 min of treatment while the Fourier-Transform Infrared spectroscopy (FT-IR) was performed to verify the occurrence of organic sorption on m-ZVI surface. The scanning electron microscope (SEM) images before and after the reaction illustrated morphological changes on m-ZVI surface. The detoxification of the treated solution was demonstrated using phytotoxicity test.
Keywords	Fourier transform infrared spectroscopy ; aqueous solutions ; decolorization ; dyes ; hydrogen peroxide ; hydroxyl radicals ; iron ; monitoring ; pH ; phytotoxicity ; scanning electron microscopes ; scanning electron microscopy ; sorption ; transmission electron microscopy ; ultraviolet-visible spectroscopy
Language	English
Dates of publication	2019-06
Size	p. 18392-18402.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-019-04929-1
Database	NAL-Catalogue (AGRICOLA)

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Article: A Difficult Recurrent Hypertrophic Pyloric Stenosis.

Louati, Hamdi / Zitouni, Hayet / Belhajmansour, Manel / Dhaou, Mahdi Ben / Jalouli, Mohamed / Mhiri, Riadh / Kallel, Rim / Boudawara, Tahya

Journal of neonatal surgery

2017 Volume 6, Issue 2, Page(s) 48

Language	English
Publishing date	2017-04-15
Publishing country	Pakistan
Document type	Journal Article
ISSN	2226-0439
ISSN	2226-0439
DOI	10.21699/jns.v6i2.484
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Article: A halotolerant laccase from

Mtibaà, Rim / de Eugenio, Laura / Ghariani, Bouthaina / Louati, Ibtihel / Belbahri, Lasaad / Nasri, Moncef / Mechichi, Tahar

3 Biotech

2017 Volume 7, Issue 5, Page(s) 329

Abstract: A novel fungal laccase produced by the ... ...

Abstract	A novel fungal laccase produced by the ascomycete
Language	English
Publishing date	2017-09-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-017-0973-5
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Article ; Online: Polymorphisms in XRCC1, ERCC2, and ERCC3 DNA repair genes, CYP1A1 xenobiotic metabolism gene, and tobacco are associated with bladder cancer susceptibility in Tunisian population.

Feki-Tounsi, Molka / Khlifi, Rim / Louati, Ibtihel / Fourati, Mohamed / Mhiri, Mohamed-Nabil / Hamza-Chaffai, Amel / Rebai, Ahmed

Environmental science and pollution research international

2017 Volume 24, Issue 28, Page(s) 22476–22484

Abstract: Other than the established environmental risk factors associated with bladder cancer (BC), little is known about the genetic variations determining the individual susceptibility of this complex disease. This study aimed to investigate the relationship of ...

Abstract	Other than the established environmental risk factors associated with bladder cancer (BC), little is known about the genetic variations determining the individual susceptibility of this complex disease. This study aimed to investigate the relationship of BC with environmental agents and polymorphisms in XRCC1, ERCC2, and ERCC3 DNA repair genes and CYP1A1, CYP2D6, NAT1, and NAT2 xenobiotic metabolism genes through a hospital-based case-control study in Tunisia. The selection of the single nucleotide polymorphisms (SNPs) (rs25487, rs 13181, rs415407, rs446421, rs1058172, rs4921880, and rs1208) was performed using the dbSNP database. DNA genotyping was determined by PCR-RFLP after DNA extraction from whole blood. The risks of BC associated with every polymorphism as well as the studied environmental factors were estimated by multivariate-adjusted logistic regression using R software. In addition, gene-gene interactions were analyzed using generalized multifactor dimensionality reduction (GMDR) methods. Results showed that tobacco smoking and chewing parameters were significantly associated with BC risk. Single-gene variant analysis showed significant associations of the TT genotype of CYP1A1 and the rare GG genotype of ERCC2 with bladder cancer susceptibility (OR = 1.34, 95% CI 1.22-1.40, P < 0.0001). According to GMDR analysis, our findings indicated a significant association between BC and gene-gene interaction among the CYP1A1, ERCC3, and XRCC1. The present results suggest a potential role of XRCC1, ERCC2, ERCC3, and CYP1A1 besides tobacco intake in susceptibility to BC.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Arylamine N-Acetyltransferase ; Case-Control Studies ; Cytochrome P-450 CYP1A1/genetics ; DNA Helicases/genetics ; DNA Repair ; DNA-Binding Proteins/genetics ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Middle Aged ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; Smoking ; Nicotiana ; Tunisia ; Urinary Bladder Neoplasms/genetics ; X-ray Repair Cross Complementing Protein 1/genetics ; Xeroderma Pigmentosum Group D Protein/genetics ; Young Adult
Chemical Substances	DNA-Binding Proteins ; X-ray Repair Cross Complementing Protein 1 ; XRCC1 protein, human ; XPBC-ERCC-3 protein (146045-44-5) ; CYP1A1 protein, human (EC 1.14.14.1) ; Cytochrome P-450 CYP1A1 (EC 1.14.14.1) ; Arylamine N-Acetyltransferase (EC 2.3.1.5) ; NAT2 protein, human (EC 2.3.1.5) ; DNA Helicases (EC 3.6.4.-) ; Xeroderma Pigmentosum Group D Protein (EC 3.6.4.12) ; ERCC2 protein, human (EC 5.99.-)
Language	English
Publishing date	2017-08-12
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-017-9767-x
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Article ; Online: Predictive value of C-reactive protein, ultrasound and Alvarado score in acute appendicitis: a prospective pediatric cohort.

Zouari, Mohamed / Jallouli, Mohamed / Louati, Hamdi / Kchaou, Rim / Chtourou, Rahma / Kotti, Ahmed / Dhaou, Mahdi Ben / Zitouni, Hayet / Mhiri, Riadh

The American journal of emergency medicine

2016 Volume 34, Issue 2, Page(s) 189–192

Abstract: Purpose: To evaluate whether C-reactive protein (CRP) level and ultrasound (US) results on admission could aid the diagnostic accuracy of Alvarado score.: Methods: A prospective study was performed on children <14 years admitted for suspected acute ... ...

Abstract	Purpose: To evaluate whether C-reactive protein (CRP) level and ultrasound (US) results on admission could aid the diagnostic accuracy of Alvarado score. Methods: A prospective study was performed on children <14 years admitted for suspected acute appendicitis. Patients were categorized into three groups based on the Alvarado score: group I: score 7-10, group II: score 5-6, group III: score 0-4. Results: The difference between predictive values of Alvarado score alone and Alvarado score with CRP was not statically significant. The PPV increased from 74.29% (Alvarado score and CRP) to 93.75% (Alvarado score and US) in group 1 (P = .001) and the NPV increased from 64.86 and 79.69% (Alvarado score and CRP) to 82.6 and 88.2% (Alvarado score and US) in group 2 (P = .01) and group 3 (P = .001), respectively. Conclusions: Alvarado score and ultrasound taken together improve the predictive value of diagnosing acute appendicitis in children.
MeSH term(s)	Adolescent ; Appendicitis/blood ; Appendicitis/diagnostic imaging ; C-Reactive Protein/metabolism ; Child ; Child, Preschool ; Female ; Humans ; Male ; Predictive Value of Tests ; Prospective Studies ; Sensitivity and Specificity ; Ultrasonography
Chemical Substances	C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2016-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	605890-5
ISSN	1532-8171 ; 0735-6757
ISSN (online)	1532-8171
ISSN	0735-6757
DOI	10.1016/j.ajem.2015.10.004
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