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Article ; Online: Yi-Qi-Huo-Xue decoction alleviates intracerebral hemorrhage injury through inhibiting neuronal autophagy of ipsilateral cortex via BDNF/TrkB pathway.

Han, Dan / Chang, Xinyue / Xu, Dan / Shen, Jizhong / Fan, Ali / Wang, Meihua / Li, Dingran / Chen, Xiangkai / Wang, Cheng / Wu, Yi / Yang, Zhaocong / Li, Jian / Wang, Siliang

Phytomedicine : international journal of phytotherapy and phytopharmacology

2024 Volume 128, Page(s) 155438

Abstract: Background: Yi-Qi-Huo-Xue Decoction (YQHXD), a traditional Chinese medicine formula, has ...

Abstract	Background: Yi-Qi-Huo-Xue Decoction (YQHXD), a traditional Chinese medicine formula, has demonstrated efficacy in the clinical treatment of intracerebral hemorrhage (ICH) for over a decade. Nevertheless, the precise pharmacotherapeutic compounds of YQHXD capable of penetrating into cerebral tissue and the pharmacological underpinnings of YQHXD remain ambiguous. Methods: The active components of YQHXD in rat brains was analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The potential targets, pathways and biological progresses of YQHXD ameliorating ICH induced injury was predicted by network pharmacology. Moreover, collagenase-induced ICH rat model, primary cortex neurons exposed to hemin and molecular docking were applied to validate the molecular mechanisms of YQHXD. Results: Eleven active components of YQHXD were identified within the brains. Employing the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, our investigation concentrated on the roles of autophagy and the BDNF/TrkB signaling pathway in the pharmacological context. The pharmacological results revealed that YQHXD alleviated neurological dysfunction, brain water content, brain swelling, and pathological injury caused by ICH. Meanwhile, YQHXD inhibited autophagy influx and autophagosome in vivo, and regulated cortex neuronal autophagy and TrkB/BDNF pathway both in vivo and in vitro. Subsequently, N-acetyl serotonin (NAS), a selective TrkB agonist, was employed to corroborate the significance of the BDNF/TrkB pathway in this process. The combination of NAS and YQHXD did not further enhance the protective efficacy of YQHXD in ICH rats. Additionally, outcomes of molecular docking analysis revealed that nine compounds of YQHXD exhibited potential regulatory effects on TrkB. Conclusions: Ipsilateral neuronal autophagy and BDNF/TrkB pathway were activated 72 h after ICH. YQHXD effectively resisted injury induced by ICH, which was related with suppression of ipsilateral neuronal autophagy via BDNF/TrkB pathway. This study provides novel insights into the therapeutic mechanisms of traditional Chinese medicine in the context of ICH treatment.
MeSH term(s)	Animals ; Brain-Derived Neurotrophic Factor/metabolism ; Cerebral Hemorrhage/drug therapy ; Drugs, Chinese Herbal/pharmacology ; Autophagy/drug effects ; Male ; Rats, Sprague-Dawley ; Neurons/drug effects ; Molecular Docking Simulation ; Rats ; Signal Transduction/drug effects ; Receptor, trkB/metabolism ; Cerebral Cortex/drug effects ; Cerebral Cortex/metabolism ; Disease Models, Animal ; Neuroprotective Agents/pharmacology
Chemical Substances	Brain-Derived Neurotrophic Factor ; Drugs, Chinese Herbal ; Ntrk2 protein, rat (EC 2.7.10.1) ; Receptor, trkB (EC 2.7.10.1) ; Bdnf protein, rat ; Neuroprotective Agents
Language	English
Publishing date	2024-02-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2024.155438
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Zs.A 4115: Show issues

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Article ; Online: Yi-Shen-Hua-Shi granule ameliorates diabetic kidney disease by the "gut-kidney axis".

Han, Cong / Shen, Zhen / Cui, Tao / Ai, Shan-Shan / Gao, Ran-Ran / Liu, Yao / Sui, Gui-Yuan / Hu, Hong-Zhen / Li, Wei

Journal of ethnopharmacology

2023 Volume 307, Page(s) 116257

Abstract: Ethnopharmacological relevance: Yi-Shen-Hua-Shi (YSHS) granule is an effective prescription widely ...

Abstract	Ethnopharmacological relevance: Yi-Shen-Hua-Shi (YSHS) granule is an effective prescription widely used in traditional Chinese medicine to treat diabetic kidney disease (DKD), its exact efficacy in treating DKD has been confirmed but the underlying regulatory mechanism has not been fully elucidated. Aim of the study: To explore the mechanism by which YSHS granule regulates intestinal flora and serum metabolites and then regulates renal mRNA expression through the "gut-kidney axis", so as to improve DKD. Materials and methods: 40 rats were divided into five groups: Normal group (N) (normal saline), model group (M) (STZ + normal saline), YSHS granule low-dose group (YL) (STZ + 2.27 g kg Results: In group M, blood glucose, blood lipid and proteinuria were increased, inflammation, oxidative stress and renal function were aggravated, with the proliferation of mesangial matrix, vacuolar degeneration of renal tubules, accumulation of collagen and lipid, and increased intestinal permeability, and YSHS granule and valsartan improved these disorders to varying degrees. High dose of YSHS granule improved the diversity and abundance of flora, decreased the F/B value, greatly increased the abundance of Lactobacillus and Lactobacillus_murinus, and decreased the abundance of Prevoella UCG_001. 14 target metabolites of YSHS granule were identified, which were mainly enriched in 20 KEGG pathways, such as Glycerophospholipid metabolism, Sphingolipid metabolism and Phenylalanine, tyrosine and tryptophan biosynthesis. 96 target mRNAs of YSHS granule were also identified. The enriched top 20 pathways were closely related to glucose and lipid metabolism, of which a total of 21 differential mRNAs were expressed. Further correlation analysis revealed that Lactobacillus, Lactobacillus_murinus and Prevotella UCG_001 were highly correlated with Glycerophospholipid metabolism, Sphingolipid metabolism and Phenylalanine, tyrosine and tryptophan biosynthesis pathways. At the same time, 6 pathways including Glycerophospholipid metabolism, Arachidonic acid metabolism, Purine metabolism, Primary bile acid biosynthesis, Ascorbate and aldarate metabolism and Galactose metabolism were co-enriched by the target metabolites and the target mRNAs of YSHS granule, including 7 differential metabolites such as phosphatidylethanolamine and 7 differential genes such as Adcy3. The 7 differential metabolites had high predictive value of AUC, and the validation of 7 differential genes were highly consistent with the sequencing results. Conclusion: YSHS granule could improve DKD through the "gut-kidney axis". Lactobacillus and Lactobacillus_murinus were the main driving forces. 6 pathways related to glucose and lipid metabolism, especially Glycerophospholipid metabolism, may be an important follow-up response and regulatory mechanism.
MeSH term(s)	Animals ; Rats ; Blood Glucose ; Chromatography, Liquid ; Diabetes Mellitus ; Diabetic Nephropathies ; Glucose ; Glycerophospholipids ; Kidney/physiology ; Saline Solution ; Sphingolipids ; Tandem Mass Spectrometry ; Tryptophan ; Valsartan ; Herbal Medicine
Chemical Substances	Blood Glucose ; Glucose (IY9XDZ35W2) ; Glycerophospholipids ; Saline Solution ; Sphingolipids ; Tryptophan (8DUH1N11BX) ; Valsartan (80M03YXJ7I)
Language	English
Publishing date	2023-02-12
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116257
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Zs.A 1494: Show issues

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Article ; Online: Clinical Research into Treating Unexplained Recurrent Spontaneous Abortion during Early Pregnancy with the Qing Yi Tiao Mian Formula.

Shen, Ming-Jie / Pan, Ding-Chen / Du, Le / Jiang, Guo-Jing

Explore (New York, N.Y.)

2022 Volume 19, Issue 1, Page(s) 52–57

Abstract: Objective: The present study aims to analyze the clinical effect of the Qing Yi Tiao Mian (QYTM ...

Abstract	Objective: The present study aims to analyze the clinical effect of the Qing Yi Tiao Mian (QYTM) formula on unexplained recurrent spontaneous abortion (URSA) during early pregnancy and the immune balance of T lymphocytes. Methods: With their consent, 45 patients with URSA in weeks 4-9 of pregnancy were separated into three groups, i.e., the conventional fetal protection (n = 15), prednisone treatment (n = 10), and QYTM formula treatment (n = 20) groups. These patients received treatment once they had been diagnosed with an intrauterine pregnancy. The conventional fetal protection group was given progesterone (20 ∼ 40 mg daily injection) for four weeks. The prednisone treatment group was given progesterone (20 ∼ 40 mg daily injection) + prednisone (5 mg/d) for four weeks. The QYTM formula treatment group was given progesterone (20 ∼ 40 mg daily injection) + QYTM formula (one dose per day) for four weeks. In addition, women who had previously had a normal pregnancy were enrolled as a control group (n = 18). The success rate of the pregnancy in the first trimester was observed in each group, and the proportion of T lymphocytes in the peripheral blood before and after treatment was recorded. Results: Among the 20 patients with URSA in the QYTM formula treatment group, 19 remained pregnant. Thus, the success rate during early pregnancy was 95%, which was significantly higher than the conventional fetal protection (53.33%) and prednisone treatment (70%) groups. The CD8+ T and natural killer (NK) cells population in the URSA groups was higher compared with the control group (P < 0.01). The QYTM formula treatment significantly decreased the ratio of CD8+ T lymphocytes (P < 0.01) and NK cells (P < 0.01). Conclusion: The QYTM formula significantly decreased the spontaneous abortion rate in patients with URSA during early pregnancy. The mechanism may be closely related to the inhibition of the killer lymphocytes' proliferation by CD8+ T lymphocytes and NK cells.
MeSH term(s)	Pregnancy ; Humans ; Female ; Progesterone/therapeutic use ; Prednisone ; Abortion, Habitual/drug therapy ; Killer Cells, Natural
Chemical Substances	Progesterone (4G7DS2Q64Y) ; Prednisone (VB0R961HZT)
Language	English
Publishing date	2022-03-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2183945-1
ISSN	1878-7541 ; 1550-8307
ISSN (online)	1878-7541
ISSN	1550-8307
DOI	10.1016/j.explore.2022.03.009
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Zs.A 6635: Show issues

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Article: Yi-Zhi-Fang-Dai Formula Protects against A

Liu, Lumei / Wan, Wenbin / Chen, Wenjing / Chan, Yuanjin / Shen, Qi / Li, Yaming

Evidence-based complementary and alternative medicine : eCAM

2016 Volume 2016, Page(s) 8591656

Abstract: Yi-Zhi-Fang-Dai formula (YZFDF) is an experiential prescription used to cure dementia cases like ...

Abstract	Yi-Zhi-Fang-Dai formula (YZFDF) is an experiential prescription used to cure dementia cases like Alzheimer's disease (AD). In this study, the main effective compounds of YZFDF have been identified from this formula, and the neuroprotective effect against A
Language	English
Publishing date	2016-10-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2016/8591656
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Zs.A 5995: Show issues

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Article ; Online: A Pilot Study on the Effects of Yi Jin Jing Exercise for Older Men with Benign Prostatic Hyperplasia.

Liu, ZhiWei / Chen, Ting / Shen, Mingkang / Li, Kai / Ma, ChunJie / Ketlhoafetse, Antonnette / Liu, XiangYun

Journal of aging and physical activity

2020 Volume 28, Issue 6, Page(s) 822–827

Abstract: ... both the physical and mental health of older men. In order to determine the efficiency of Chinese Qigong Yi Jin Jing ... on prostate health in older individuals, thirty participants were randomized into either an Yi Jin Jing group ... n = 15) or a control group (n = 15). After the 6-month intervention, the Yi Jin Jing group showed ...

Abstract	Benign prostatic hyperplasia and its associated lower urinary tract symptoms seriously affect both the physical and mental health of older men. In order to determine the efficiency of Chinese Qigong Yi Jin Jing on prostate health in older individuals, thirty participants were randomized into either an Yi Jin Jing group (n = 15) or a control group (n = 15). After the 6-month intervention, the Yi Jin Jing group showed a significant decrease in international prostate symptom score and a significant increase in maximal urinary flow rate (compared with the control group p = .005, p = .001, respectively). Also, testosterone level increased and estrogen/testosterone ratio decreased in the Yi Jin Jing group (compared with the baseline p = .004, p = .002, respectively); estrogen level and estrogen/testosterone ratio were lower in the Yi Jin Jing group (compared with the control group p = .029, p = .012, respectively). The results showed that Yi Jin Jing is a promising way to reduce the risk of benign prostatic hyperplasia-lower urinary tract symptoms in older men.
Language	English
Publishing date	2020-05-20
Publishing country	United States
Document type	Journal Article
ISSN	1543-267X
ISSN (online)	1543-267X
DOI	10.1123/japa.2019-0315
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Article ; Online: Network Pharmacology and Molecular Docking Study on the Potential Mechanism of Yi-Qi-Huo-Xue-Tong-Luo Formula in Treating Diabetic Peripheral Neuropathy.

Lin, Yixuan / Shen, Chuqiao / Wang, Fanjing / Fang, Zhaohui / Shen, Guoming

Journal of diabetes research

2021 Volume 2021, Page(s) 9941791

Abstract: Objective: To investigate the potential mechanism of action of Yi-Qi-Huo-Xue-Tong-Luo formula ...

Abstract	Objective: To investigate the potential mechanism of action of Yi-Qi-Huo-Xue-Tong-Luo formula (YQHXTLF) in the treatment of diabetic peripheral neuropathy (DPN). Methods: Network pharmacology and molecular docking techniques were used in this study. Firstly, the active ingredients and the corresponding targets of YQHXTLF were retrieved using the Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform; subsequently, the targets related to DPN were retrieved using GeneCards, Online Mendelian Inheritance in Man (OMIM), Pharmgkb, Therapeutic Target Database (TTD) and Drugbank databases; the common targets of YQHXTLF and DPN were obtained by Venn diagram; afterwards, the "YQHXTLF Pharmacodynamic Component-DPN Target" regulatory network was visualized using Cytoscape 3.6.1 software, and Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on the potential targets using R 3.6.3 software. Finally, molecular docking of the main chemical components in the PPI network with the core targets was verified by Autodock Vina software. Results: A total of 86 active ingredients and 229 targets in YQHXTLF were screened, and 81 active ingredients and 110 targets were identified to be closely related to diabetic peripheral neuropathy disease. PPI network mapping identified TP53, MAPK1, JUN, and STAT3 as possible core targets. KEGG pathway analysis showed that these targets are mostly involved in AGE-RAGE signaling pathway in diabetic complications, TNF signaling pathway, and MAPK signaling pathway. The molecular docking results showed that the main chemical components of YQHXTLF have a stable binding activity to the core pivotal targets. Conclusion: YQHXTLF may act on TP53, MAPK1, JUN, and STAT3 to regulate inflammatory response, apoptosis, or proliferation as a molecular mechanism for the treatment of diabetic peripheral neuropathy, reflecting its multitarget and multipathway action, and providing new ideas to further uncover its pharmacological basis and mechanism of action.
MeSH term(s)	Angelica sinensis ; Astragalus Plant ; Chrysanthemum ; Diabetic Neuropathies/drug therapy ; Dioscorea ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Glycation End Products, Advanced/drug effects ; Glycation End Products, Advanced/metabolism ; Humans ; Mitogen-Activated Protein Kinase 1/drug effects ; Mitogen-Activated Protein Kinase 1/metabolism ; Molecular Docking Simulation ; Network Pharmacology ; Proto-Oncogene Proteins c-jun/drug effects ; Proto-Oncogene Proteins c-jun/metabolism ; Pueraria ; Receptor for Advanced Glycation End Products/drug effects ; Receptor for Advanced Glycation End Products/metabolism ; STAT3 Transcription Factor/drug effects ; STAT3 Transcription Factor/metabolism ; Signal Transduction ; Tumor Suppressor Protein p53/drug effects ; Tumor Suppressor Protein p53/metabolism
Chemical Substances	Drugs, Chinese Herbal ; Glycation End Products, Advanced ; Proto-Oncogene Proteins c-jun ; Receptor for Advanced Glycation End Products ; STAT3 Transcription Factor ; Tumor Suppressor Protein p53 ; Mitogen-Activated Protein Kinase 1 (EC 2.7.11.24)
Language	English
Publishing date	2021-05-28
Publishing country	England
Document type	Journal Article
ZDB-ID	2711897-6
ISSN	2314-6753 ; 2314-6753
ISSN (online)	2314-6753
ISSN	2314-6753
DOI	10.1155/2021/9941791
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Article ; Online: Yi-Qi-Jian-Pi formula modulates the PI3K/AKT signaling pathway to attenuate acute-on-chronic liver failure by suppressing hypoxic injury and apoptosis in vivo and in vitro.

Tang, Li / Wang, Feixia / Xiao, Lingyan / Shen, Min / Xia, Siwei / Zhang, Zili / Zhang, Feng / Zheng, Shizhong / Tan, Shanzhong

Journal of ethnopharmacology

2021 Volume 280, Page(s) 114411

Abstract: ... threatens human lives. Yi-Qi-Jian-Pi formula (YQJPF) is a herbal compound commonly used to treat ...

Abstract	Ethnopharmacological relevance: Acute-on-chronic liver failure (ACLF) is a key complication of chronic hepatitis, with a relatively high mortality rate and limited treatment options, which dramatically threatens human lives. Yi-Qi-Jian-Pi formula (YQJPF) is a herbal compound commonly used to treat liver failure. Aim of the study: The purpose of this research is to discuss the potential molecular biological effect and mechanism of YQJPF in ACLF. Materials and methods: In this study, we created a rat model of ACLF by CCl Results: Our research demonstrated that, after YQJPF treatment, hepatocyte injury in rats was relieved. Bioinformatics analysis showed that PI3K/AKT, HIF-1, mitochondrial apoptosis pathways played prominent roles. YQJPF promoted HIF-1α protein expression and exerted protective effects against hypoxic injury, simultaneously reducing mitochondrial ROS production, suppressing hepatocyte apoptosis. Furthermore, we showed that YQJPF accelerates PI3K/AKT pathway activation, a known broad-spectrum inhibitor of PI3K. LY294002, which was used for reverse verification, suppressed the effect of YQJPF on hypoxic injury and ROS-mediated hepatocyte apoptosis. Conclusions: YQJPF ameliorates liver injury by suppressing hypoxic injury and ROS-mediated hepatocyte apoptosis by modulating the PI3K/AKT pathway.
MeSH term(s)	Animals ; Chemical and Drug Induced Liver Injury/drug therapy ; Chemical and Drug Induced Liver Injury/metabolism ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/therapeutic use ; End Stage Liver Disease/chemically induced ; End Stage Liver Disease/drug therapy ; Gene Expression Regulation/drug effects ; Hepatocytes/drug effects ; Hepatocytes/metabolism ; Liver/drug effects ; Liver/metabolism ; Male ; Methylprednisolone/therapeutic use ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Drugs, Chinese Herbal ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Methylprednisolone (X4W7ZR7023)
Language	English
Publishing date	2021-07-12
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114411
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Zs.A 1494: Show issues

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Article ; Online: Metabonomic analysis of biochemical changes in the plasma and urine of carrageenan-induced rats after treatment with Yi-Guan-Jian decoction.

Shui, Sufang / Shen, Shujie / Huang, Rongqing / Xiao, Bingkun / Yang, Jianyun

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2016 Volume 1033-1034, Page(s) 80–90

Abstract: ... of Yi-Guan-Jian (YGJ) decoction and explore its potential anti-inflammatory mechanism. Rat acute ... treated group (AG) and Yi-Guan-Jian decoction group (YGJ), aimed at finding out the potential biomarkers ...

Abstract	The urinary and plasma metabonomics method based on a Agilent-1200 LC system coupled to an Agilent-6410 mass spectrometry (HPLC-MS/MS) had been established to investigate the anti-inflammatory activity of Yi-Guan-Jian (YGJ) decoction and explore its potential anti-inflammatory mechanism. Rat acute inflammation was induced by subcutaneous injection of carrageenan in hind paws. Multivariate statistical approaches, such as principal component analysis (PCA), partial least-squares discriminant analysis (PLS-DA) and XCMS online software were used to distinguish normal control group (NG), model group (MG), aspirin-treated group (AG) and Yi-Guan-Jian decoction group (YGJ), aimed at finding out the potential biomarkers. There was a clear separation among the four groups in PCA model. Twenty-five potential biomarkers had been identified using PCA, PLS-DA and XCMS online software. Lastly, we had an enrichment for the related metabolic pathways and screened out the pathways that influence the organism a lot in MetPA, then five mainly metabolism: tryptophan metabolism, lipid metabolism, oxidative stress, glyoxylate and dicarboxylate metabolism, taurine and hypotaurine metabolism were found. In this study, YGJ showed good anti-inflammatory effects and it could suppress the changes of pathologic inflammatory cytokines of carrageenan-induced rat paw edema (CIE). There might be a correlation between these results and the regulation of the disturbed metabolites in urine and plasma. This study demonstrates that metabonomics is a powerful methodology to gain insight into the mechanisms of traditional Chinese medicine (TCM) formula in therapy.
MeSH term(s)	Animals ; Biomarkers/blood ; Biomarkers/urine ; Carrageenan/toxicity ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal/pharmacology ; Limit of Detection ; Male ; Metabolome/drug effects ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results ; Tandem Mass Spectrometry
Chemical Substances	Biomarkers ; Drugs, Chinese Herbal ; Yi Guan Jian Decoction ; Carrageenan (9000-07-1)
Language	English
Publishing date	2016-10-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2016.08.003
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Zs.A 813: Show issues

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Article ; Online: Genetic analysis of 15 mtDNA SNP loci in Chinese Yi ethnic group using SNaPshot minisequencing.

Hu, Chun-Ting / Yan, Jiang-Wei / Chen, Feng / Zhang, Qing-Xia / Wang, Hong-Dan / Yin, Cai-Yong / Fan, Han-Ting / Hu, Ling-Li / Shen, Chun-Mei / Meng, Hao-Tian / Zhang, Yu-Dang / Wang, Hui / Zhu, Bo-Feng

Gene

2016 Volume 576, Issue 1 Pt 1, Page(s) 105–108

Abstract: ... collected from 99 unrelated individuals of the Yi ethnic minority group in Yunnan Province. We have ... sequences of Yi group and other 9 populations worldwide and drew a Neighbor-Joining tree based on the shared ... 12 mtDNA SNP loci, which demonstrated a close relationship between Yi and Bai groups. In conclusion ...

Abstract	SNaPshot minisequencing is a rapid and robust methodology based on a single base extension with a labeled ddNTP. The present study detected 15 selected SNPs in the mitochondrial DNA (mtDNA) control and coding regions by minisequencing methodology using SNaPshot for forensic purpose. The samples were collected from 99 unrelated individuals of the Yi ethnic minority group in Yunnan Province. We have predominantly found high-frequency transitions (91.7%) and a significantly lower frequency of transversions (8.3%). The nt152, 489, 8701, 10,398, 16,183, and 16,362 loci were highly polymorphic, while the nt231, 473 and 581 loci were not polymorphic in the studied population. Based on these 15 SNPs, a total of 28 mtDNA haplotypes were defined in 99 individuals with the haplotype diversity of 0.9136. Also, we compared the mtDNA sequences of Yi group and other 9 populations worldwide and drew a Neighbor-Joining tree based on the shared 12 mtDNA SNP loci, which demonstrated a close relationship between Yi and Bai groups. In conclusion, the analysis of the 15 selected SNPs increases considerably the discrimination power of mtDNA. Moreover, the SNaPshot minisequencing method could quickly detect mtDNA SNPs, and is economical and sensitive. The set of selected 15 SNPs is highly informative and is capable for anthropology genetic analysis.
MeSH term(s)	Asian Continental Ancestry Group ; China ; DNA, Mitochondrial/genetics ; Female ; Genetic Loci ; Humans ; Male ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA/methods
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2016-01-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2015.09.071
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Zs.A 1357: Show issues

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Article: Yi-Qi-Jian-Pi formula modulates the PI3K/AKT signaling pathway to attenuate acute-on-chronic liver failure by suppressing hypoxic injury and apoptosis in vivo and in vitro

Tang, Li / Wang, Feixia / Xiao, Lingyan / Shen, Min / Xia, Siwei / Zhang, Zili / Zhang, Feng / Zheng, Shizhong / Tan, Shanzhong

Journal of ethnopharmacology. 2021 Nov. 15, v. 280

2021

Abstract: ... high mortality rate and limited treatment options, which dramatically threatens human lives. Yi-Qi-Jian ...

Abstract	Acute-on-chronic liver failure (ACLF) is a key complication of chronic hepatitis, with a relatively high mortality rate and limited treatment options, which dramatically threatens human lives. Yi-Qi-Jian-Pi formula (YQJPF) is a herbal compound commonly used to treat liver failure.The purpose of this research is to discuss the potential molecular biological effect and mechanism of YQJPF in ACLF.In this study, we created a rat model of ACLF by CCl₄-, LPS- and D-Galactosamine (D-Gal) and an in vitro model of LPS-induced hepatocyte damage. The specific components of YQJPF and potential mechanism were explored based on bioinformatics analyses. Furthermore, we verified the effect of YQJPF on ACLF using immunohistochemistry, RT-qPCR, western blotting, and flow cytometry.Our research demonstrated that, after YQJPF treatment, hepatocyte injury in rats was relieved. Bioinformatics analysis showed that PI3K/AKT, HIF-1, mitochondrial apoptosis pathways played prominent roles. YQJPF promoted HIF-1α protein expression and exerted protective effects against hypoxic injury, simultaneously reducing mitochondrial ROS production, suppressing hepatocyte apoptosis. Furthermore, we showed that YQJPF accelerates PI3K/AKT pathway activation, a known broad-spectrum inhibitor of PI3K. LY294002, which was used for reverse verification, suppressed the effect of YQJPF on hypoxic injury and ROS-mediated hepatocyte apoptosis.YQJPF ameliorates liver injury by suppressing hypoxic injury and ROS-mediated hepatocyte apoptosis by modulating the PI3K/AKT pathway.
Keywords	animal models ; apoptosis ; bioinformatics ; chronic hepatitis ; humans ; immunohistochemistry ; liver ; liver failure ; mitochondria ; mortality ; phosphatidylinositol 3-kinase ; protein synthesis ; traditional medicine
Language	English
Dates of publication	2021-1115
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114411
Database	NAL-Catalogue (AGRICOLA)

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