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Article ; Online: The Medicinal Effects of Different Solvent Extracts of Pyracantha Coccinea Roem. Fruits

Neslihan Tekin KARACAER

Bezmiâlem Science, Vol 11, Iss 1, Pp 23-

Heavy Metal Content, Antioxidant, and Antimicrobial Properties

2023 Volume 31

Abstract: Objective:The current investigation has been conducted to assess the total flavonoid content, total phenolic content, heavy metal composition, and antioxidant and antimicrobial activities of Pyracantha coccinea Roem. fruit extracts prepared with ... ...

Abstract	Objective:The current investigation has been conducted to assess the total flavonoid content, total phenolic content, heavy metal composition, and antioxidant and antimicrobial activities of Pyracantha coccinea Roem. fruit extracts prepared with different solvents.Methods:Ethanol, diethyl ether, and hot water extraction were used as solvents to prepare the extract of Pyracantha coccinea Roem. fruit. Total phenolic ingredient was assessed by Folin- Ciocalteu assay, and the total ingredient of flavonoids was measured spectrophotometrically via AlCl3 assay. The antioxidant activities of the extracts were investigated via free radical scavenging assays, DPPH, and ABTS. The fruits were analyzed by Inductively Coupled Plasma/Mass Spectrometer to determine heavy metal content. The antimicrobial activities of the extracts were investigated using agar well diffusion method against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and Candida albicans.Results:It was determined that the total flavonoid ingredient, total phenolic ingredient, ABTS and DPPH activities of the hot water extract were significantly higher than the other fractions. These parameters were found to be significantly higher in ethanol extract compared to ether extract. All extracts exhibited antimicrobial activity against Bacillus cereus and Pseudomonas aeruginosa while the hot water fraction exhibited the highest antibacterial effect against Pseudomonas aeruginosa. It was determined that Cr, Co, Ni, and Cu contents exceeded the toxicity thresholds that might be found in plants.Conclusion:These results suggest that Pyracantha coccinea Roem. fruit may be considered as a natural source of antioxidants and antimicrobial agents.
Keywords	abts ; antimicrobial activity ; antioxidant activity ; dpph ; pyracantha coccinea roem ; Medicine (General) ; R5-920
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Galenos Publishing House
Document type	Article ; Online
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Article ; Online: In vitro AND in silico EVALUATION OF THYMOQUINONE AS POTENTIAL ANTICANCER AGENT IN HUMAN ACUTE MYELOID LEUKEMIA HL-60 CELLS

Neslihan Tekin Karacaer

Trakya University Journal of Natural Sciences, Vol 23, Iss 1, Pp 53-

2022 Volume 63

Abstract: This study aims to explore the cytotoxic, apoptotic and autophagic effects of thymoquinone on human acute myeloid leukemia. The cytotoxic effects of thymoquinone were determined with 3-(4, 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) ...

Abstract	This study aims to explore the cytotoxic, apoptotic and autophagic effects of thymoquinone on human acute myeloid leukemia. The cytotoxic effects of thymoquinone were determined with 3-(4, 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) tests. B-cell lymphoma 2 associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), caspase 3, mammalian target of rapamycin (mTOR), phosphatidylinositol-3-kinase (PI3K), and protein kinase B (AKT) gene expression analyzes were studied with quantitative real-time polymerase chain reaction (qRT-PCR). AutoDock Tools 4.2 software was applied to research the potential binding of thymoquinone in the active sites of Bax, Bcl-2, caspase 3, mTOR, PI3K, and AKT proteins. Thymoquinone caused a cytotoxic effect on HL-60 cells (Human leukemia cell line) with a value of 16.35 µM. Bcl-2 expression was decreased in all concentrations applied compared to the control. A decrease in caspase 3 expression level was detected in the cells treated with 10 µM, 15 µM, and 25 µM thymoquinone compared to the control. Thymoquinone induced an important decrease in mTOR and PI3K expressions compared to the control at all doses, while AKT decreased at a dose of 15 µM. The docking outcomes showed that thymoquinone interacts with the active site amino acids of apoptotic and autophagic proteins via hydrophobic interactions and hydrogen bonding. The present findings suggest that thymoquinone can stimulate autophagy by prevention of PI3K/AKT/mTOR pathway in HL-60 cells and may become a new target for the therapy of acute myeloid leukemia.
Keywords	aml ; apoptosis ; autophagy ; molecular docking ; pi3k/akt/mtor ; thymoquinone ; Science (General) ; Q1-390
Subject code	500
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	Trakya University
Document type	Article ; Online
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Article: The Effects of Job Stress on Burnout and Turnover Intention: The Moderating Effects of Job Security and Financial Dependency.

Üngüren, Engin / Onur, Neslihan / Demirel, Hüsne / Tekin, Ömer Akgün

Behavioral sciences (Basel, Switzerland)

2024 Volume 14, Issue 4

Abstract: 1) Background: The hospitality industry is known for exposing employees to work stress, which can lead to work-related burnout and high turnover rates. This study aims to examine the relationships between work stress, work-related burnout, and turnover ... ...

Abstract	(1) Background: The hospitality industry is known for exposing employees to work stress, which can lead to work-related burnout and high turnover rates. This study aims to examine the relationships between work stress, work-related burnout, and turnover intention. It also explores the mediating role of work-related burnout and the moderating role of job security and financial dependence. (2) Methods: A cross-sectional survey was conducted among 494 hotel employees working in five-star hotels in Belek and Manavgat, Türkiye, using a moderated mediation research model. The study found that work stress increases work-related burnout, which in turn increases turnover intention. Additionally, work-related burnout was found to mediate the relationship between work stress and turnover intention. Furthermore, it was found that perceived job security moderates the relationship between work stress levels and work-related burnout. Additionally, the variable of financial dependence was found to moderate the relationship between employees' levels of work-related burnout and their turnover intentions. Similarly, the study found that the financial dependence variable moderates the effect of work-related burnout on employees' turnover intention. Additionally, the study found that employees' perception of job security moderates the impact of work stress on work-related burnout. In conclusion, the study suggests that positive perceptions of job security can mitigate the impact of work stress on work-related burnout. Similarly, the impact of work-related burnout on turnover intention diminishes as the degree of financial dependence rises.
Language	English
Publishing date	2024-04-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2651997-5
ISSN	2076-328X
ISSN	2076-328X
DOI	10.3390/bs14040322
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Article ; Online: A Tragic Consequence of Respiratory Syncytial Virus Infection.

Barsan Kaya, Tuğba / Öztunalı, Çiğdem / Aydemir, Özge / Sürmeli Onay, Özge / Tekin, Ayşe Neslihan

Clinical pediatrics

2023 Volume 63, Issue 2, Page(s) 171–175

MeSH term(s)	Humans ; Infant ; Respiratory Syncytial Virus Infections/complications ; Respiratory Syncytial Virus Infections/diagnosis
Language	English
Publishing date	2023-10-07
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	207678-0
ISSN	1938-2707 ; 0009-9228
ISSN (online)	1938-2707
ISSN	0009-9228
DOI	10.1177/00099228231204445
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Article ; Online: Turkish validity and reliability of the lifestyle questionnaire related to cancer.

Öner, Neslihan / Borlu, Arda / Seyfeli, Mevlüde Yasemin Akşehirli / Tekin, Tuba

Revista da Associacao Medica Brasileira (1992)

2023 Volume 69, Issue 2, Page(s) 330–334

Abstract: Objective: The aim of this study was to adapt the lifestyle questionnaire related to cancer in Turkish and investigate its validity and reliability.: Methods: This methodological study was conducted on 1,196 participants. Cronbach's α was used to ... ...

Abstract	Objective: The aim of this study was to adapt the lifestyle questionnaire related to cancer in Turkish and investigate its validity and reliability. Methods: This methodological study was conducted on 1,196 participants. Cronbach's α was used to assess validity and reliability. The internal consistency was evaluated using item-total correlation. Results: The normed chi-square in this study was 5.87. The root mean square error of approximation was calculated as 0.051. The comparative fit index and the Tucker-Lewis Index were 0.83 and 0.81, respectively. The split-half method was used to test the reliability of the scale (Part 1 Cronbach's α: 0.826, Part 2 Cronbach's α: 0.812, and Adjusted Cronbach's α: 0.881). Conclusion: The Turkish version of lifestyle questionnaire related to cancer (8 subscales, 41 items) is a reliable and valid measure to evaluate lifestyle behaviors related to cancer in adults.
MeSH term(s)	Adult ; Humans ; Reproducibility of Results ; Psychometrics ; Life Style ; Surveys and Questionnaires ; Neoplasms
Language	English
Publishing date	2023-03-03
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	731969-1
ISSN	1806-9282 ; 0104-4230 ; 0004-5241 ; 0102-843X
ISSN (online)	1806-9282
ISSN	0104-4230 ; 0004-5241 ; 0102-843X
DOI	10.1590/1806-9282.20221292
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Zs.B 654: Show issues

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Article ; Online: Ten-year Single Center Experience With Colistin Therapy in NICU.

Kaya, Tuğba Barsan / Sürmeli Onay, Özge / Aydemir, Özge / Tekin, Ayşe Neslihan

The Pediatric infectious disease journal

2023

Abstract: Background: Colistin, a cationic polypeptide antibiotic of the polymyxin class has come back into use due to its potent antimicrobial activity against multidrug-resistant gram-negative bacteria and the lack of new antibiotics. The purpose of this study ... ...

Abstract	Background: Colistin, a cationic polypeptide antibiotic of the polymyxin class has come back into use due to its potent antimicrobial activity against multidrug-resistant gram-negative bacteria and the lack of new antibiotics. The purpose of this study was to assess the critically ill infants treated with colistin in our neonatal intensive care unit and to identify predisposing factors for the emergence of acute kidney injury (AKI) following colistin treatment. Methods: This was a retrospective case-control study that included infants with proven or suspected nosocomial infections in the neonatal intensive care unit of a University Hospital between January 2012 and March 2022. Over the same time period, the clinical and laboratory characteristics and outcomes of patients who received antibiotic combination with colistin were compared to patients who received antibiotic combination without colistin. Results: A total of 77 patients were in the colistin group (ColG) and 77 patients were in the control group. The demographic and clinical characteristics of the study groups were similar. In the ColG compared to the control group, hyponatremia, hypokalemia, hypophosphatemia, hypomagnesia and AKI were all more prevalent (P < 0.05). The most important finding in our study was the higher incidence of AKI and mortality in ColG, as well as the increasing nephrotoxic effect of other medications when used in conjunction with colistin. Conclusion: During colistin therapy, newborn infants must be closely monitored for AKI. Clinicians should be aware of an increased incidence of hyponatremia, hypokalemia, hypophosphatemia, hypomagnesia, AKI and its consequences in infants given colistin. As awareness increases, harmful effects will decrease.
Language	English
Publishing date	2023-10-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	392481-6
ISSN	1532-0987 ; 0891-3668
ISSN (online)	1532-0987
ISSN	0891-3668
DOI	10.1097/INF.0000000000004130
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Zs.A 1852: Show issues

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Article ; Online: Assessment of foetal ventriculomegaly from prenatal to early postnatal period: a single-centre retrospective cohort study.

Sohret, Nurullah Cihan / Tekin, Ayse Neslihan / Surmeli Onay, Ozge / Suman, Kamuran / Aydemir, Ozge / Velipasaoglu, Melih

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology

2022 , Page(s) 1–8

Abstract: The aim of this study was to evaluate the early neonatal outcomes of cases with foetal ventriculomegaly (VM) and to investigate the aetiological and prognostic factors according to the degree of VM in a single tertiary referring centre. The medical ... ...

Abstract	The aim of this study was to evaluate the early neonatal outcomes of cases with foetal ventriculomegaly (VM) and to investigate the aetiological and prognostic factors according to the degree of VM in a single tertiary referring centre. The medical records of 87 foetuses diagnosed with VM (≥10 mm) within 6 years were evaluated. Postnatal evaluation and early neonatal prognosis were determined in 39 cases divided into two groups as mild (10-15 mm, 30 cases) and severe (>15 mm, 9 cases) according to the ventricular size. The mean gestational age at which foetal VM was detected was 22 + 3 weeks. In terms of severity, severe cases of VM were more frequent in terminated pregnancies. There was no difference in gestational age, birth weight, fifth minute Apgar scores, or cord blood gases between mild and severe cases at delivery. Isolated VM was detected in 63% of mild and 22% of severe cases. In severe cases, the need for intensive care and surgery was higher than in mild cases. Antenatal VM regressed in 50% of mild cases and 22% of severe cases. Increasing knowledge about neonatal prognosis, the factors involved in aetiology, and the degree of VM will guide the management of foetal VM.IMPACT STATEMENT
Language	English
Publishing date	2022-09-23
Publishing country	England
Document type	Journal Article
ZDB-ID	604639-3
ISSN	1364-6893 ; 0144-3615
ISSN (online)	1364-6893
ISSN	0144-3615
DOI	10.1080/01443615.2022.2125295
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Zs.B 2461: Show issues

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Article ; Online: Composition of Microbiota in Transient and Mature Human Milk: Significant Changes in Large for Gestational Age Group.

Dinleyici, Meltem / Pérez-Brocal, Vicente / Arslanoglu, Sertac / Aydemir, Ozge / Sevuk Ozumut, Sibel / Tekin, Neslihan / Vandenplas, Yvan / Moya, Andrés / Dinleyici, Ener Cagri

Nutrients

2024 Volume 16, Issue 2

Abstract: The composition of the human milk (HM) microbiota and, consequently, the microorganisms that are passed on to the infant through breastfeeding, can be influenced by various factors such as the mother's health and diet, gestational age, delivery mode, ... ...

Abstract	The composition of the human milk (HM) microbiota and, consequently, the microorganisms that are passed on to the infant through breastfeeding, can be influenced by various factors such as the mother's health and diet, gestational age, delivery mode, lactation stage, method of infant feeding, and geographical location. The aim of the Human Milk-Gest Study was to compare the microbiota of transient (postpartum 7-15 days) and mature HM (postpartum 45-90 days) of 44 mothers, and to investigate any potential changes associated with preterm birth, mode of delivery, and birth weight in relation to gestational age. The data were classified into five study groups: normal spontaneous delivery-term (NS-T) newborns, cesarean delivery-term (CS-T) newborns, preterm (PT) newborns (with a gestational age of less than 37 weeks), small for gestational age (SGA) newborns, and large for gestational age (LGA) newborns. An analysis of differential abundance was conducted using ANCOM-BC to compare the microbial genera between transient and mature HM samples as well as between other study groups. A significant difference was detected between HM samples at different sampling times and between the study groups (
MeSH term(s)	Infant, Newborn ; Female ; Pregnancy ; Infant ; Humans ; Milk, Human ; Gestational Age ; Premature Birth ; Breast Feeding ; Lactation
Language	English
Publishing date	2024-01-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu16020208
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Article ; Online: Questioning the adequacy of standardized vitamin D supplementation protocol in very low birth weight infants: a prospective cohort study.

Sarıdemir, Hatice / Surmeli Onay, Ozge / Aydemir, Ozge / Tekin, Ayse Neslihan

Journal of pediatric endocrinology & metabolism : JPEM

2021 Volume 34, Issue 12, Page(s) 1515–1523

Abstract: Objectives: Preterm infants are at increased risk for vitamin D deficiency (VDD). We aimed to assess the adequacy of standardized vitamin D supplementation protocol in very low birth weight (VLBW) infants. Additionally, vitamin D status of mother/infant ...

Abstract	Objectives: Preterm infants are at increased risk for vitamin D deficiency (VDD). We aimed to assess the adequacy of standardized vitamin D supplementation protocol in very low birth weight (VLBW) infants. Additionally, vitamin D status of mother/infant couples and the associations between vitamin D status at birth and morbidities of the infants were investigated. Methods: In this single-center, prospective cohort study blood samples were collected from 55 mothers just before delivery and from their infants at birth and on the 30th day of life (DOL) for 25 hydroxy vitamin D (25OHD) measurements. Vitamin D was initiated in dose of 160 IU/kg by parenteral nutrition on the first DOL and oral vitamin D supplementation (400 IU/day) was administered when enteral feedings reached 50% of total intake or on the 15th DOL. Results: The median 25OHD levels of the infants were 16.12 (9.14-20.50) in cord blood and 36.32 (31.10-44.44) in venous blood on the 30th DOL (p<0.01). In 98% of the VLBW infants 25OHD reached sufficient levels on the 30th DOL. None of the mothers had sufficient vitamin D levels (25OHD >30 ng/mL). Maternal 25OHD levels were correlated with the 25OHD levels of the infants in cord blood (r=0.665, p<0.001). There was a significant difference in mean cord 25OHD levels between winter (13.65 ± 5.69 ng/mL) and summer seasons (19.58 ± 11.67 ng/mL) (p=0.021). No association was found between neonatal morbidity and vitamin D status. Conclusions: The results clearly show that by utilizing the current supplementation protocol, the majority of VLBW infants with deficient/insufficient serum 25OHD levels reached sufficient levels on the 30th DOL. Furthermore, vitamin D levels in mother/infant couples were found to be highly correlated.
MeSH term(s)	Adult ; Dietary Supplements/standards ; Female ; Follow-Up Studies ; Humans ; Infant, Newborn ; Infant, Premature/growth & development ; Infant, Very Low Birth Weight/growth & development ; Male ; Prognosis ; Prospective Studies ; Vitamin D/administration & dosage ; Vitamin D/analogs & derivatives ; Vitamin D/standards ; Vitamin D Deficiency/diet therapy ; Young Adult
Chemical Substances	Vitamin D (1406-16-2) ; 25-hydroxyvitamin D (A288AR3C9H)
Language	English
Publishing date	2021-08-20
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1231070-0
ISSN	2191-0251 ; 0334-018X
ISSN (online)	2191-0251
ISSN	0334-018X
DOI	10.1515/jpem-2021-0390
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Zs.A 2258: Show issues

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Article: Cerebral sinovenous thrombosis in children: clinical presentation, locations, and acquired and inherited prothrombotic risk factors.

Kar, Yeter Düzenli / Özdemir, Zeynep Canan / Çarman, Kürşat Bora / Yarar, Coşkun / Tekin, Neslihan / Bör, Özcan

The Turkish journal of pediatrics

2022 Volume 63, Issue 6, Page(s) 1028–1037

Abstract: Background: Cerebral sinovenous thrombosis (CSVT) in children is a rare and life-threatening cerebrovascular disease. Hence, we evaluated its clinical presentations, inherited and acquired prothrombotic risk factors along with the accompanying diseases, ...

Abstract	Background: Cerebral sinovenous thrombosis (CSVT) in children is a rare and life-threatening cerebrovascular disease. Hence, we evaluated its clinical presentations, inherited and acquired prothrombotic risk factors along with the accompanying diseases, the thrombosis locations as well as the outcomes of anticoagulant therapy in children with CSVT. Methods: The medical records of pediatric CSVT patients treated between January 2011 and September 2018 were analyzed retrospectively. Results: The study included 29 children, 15 boys (51.7%) and 14 girls (48.3%), with the median age being 11 years (range:3 days-17 years). The most commonly presented complaint in neonates was seizures and in the non-neonatal age groups was a headache. Also, at least one acquired and/or inherited thrombophilic risk factor was identified in 89.7% of the patients. The most commonly acquired prothrombotic risk factors along with the accompanying diseases included infections, central venous catheter, and dehydration, while the most commonly inherited thrombophilic risk factors included heterozygous factor-V Leiden mutation and elevated lipoprotein (a). The most common thrombosis location was found to be the transverse sinus. Also, none of the patients died due to the thrombotic episode. Complications included epilepsy in five patients, hydrocephalus in one patient, and intracranial hypertension in another patient. Conclusions: Clinicians need to be well aware of the inherited and acquired prothrombotic risk factors in CSVT. It should also be kept in mind that at-risk patients may also present with nonspecific signs and symptoms with no apparent neurological manifestation. The risk of acute complications and long-term sequelae can be substantially reduced if diagnosed early and initiated with appropriate treatment at the early stages.
MeSH term(s)	Child ; Child, Preschool ; Female ; Humans ; Intracranial Thrombosis/diagnosis ; Intracranial Thrombosis/epidemiology ; Intracranial Thrombosis/etiology ; Male ; Retrospective Studies ; Risk Factors ; Sinus Thrombosis, Intracranial/diagnosis ; Sinus Thrombosis, Intracranial/epidemiology ; Sinus Thrombosis, Intracranial/etiology ; Thrombosis
Language	English
Publishing date	2022-01-26
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	123487-0
ISSN	0041-4301
ISSN	0041-4301
DOI	10.24953/turkjped.2021.06.011
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