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  1. Article ; Online: Effects of antifreeze protein from

    Silva Júnior, R A / Desenzi, R / Ramires, M M S / Souza, A F / Donato, M A M / Peixoto, C A / Nascimento, T / Bartolomeu, C C / Batista, A M

    Zygote (Cambridge, England)

    2023  Volume 31, Issue 5, Page(s) 468–474

    Abstract: In the present study, the cryoprotective effects ... ...

    Abstract In the present study, the cryoprotective effects of
    MeSH term(s) Cattle ; Animals ; Vitrification ; Dimethyl Sulfoxide/pharmacology ; Cryopreservation ; Lolium ; Fertilization in Vitro ; Cryoprotective Agents/pharmacology ; Blastocyst ; Ethylene Glycol/pharmacology
    Chemical Substances Dimethyl Sulfoxide (YOW8V9698H) ; Cryoprotective Agents ; Ethylene Glycol (FC72KVT52F)
    Language English
    Publishing date 2023-06-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 1166294-3
    ISSN 1469-8730 ; 0967-1994
    ISSN (online) 1469-8730
    ISSN 0967-1994
    DOI 10.1017/S0967199423000333
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    Zs.A 3816: Show issues Location:
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  2. Article ; Online: The "epidemic" of nonsurgical cosmetic procedures providers in Brazil.

    Nascimento, Bartolomeu A / de Souza, Maria Waléria Ferreira / Motta, Flávio / Torreão, Guilherme / Barreto, Rafael Coutinho / Anlicoara, Rafael

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS

    2023  Volume 79, Page(s) 11–12

    MeSH term(s) Humans ; Brazil/epidemiology ; Cosmetic Techniques
    Language English
    Publishing date 2023-02-04
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 2217750-4
    ISSN 1878-0539 ; 1748-6815 ; 0007-1226
    ISSN (online) 1878-0539
    ISSN 1748-6815 ; 0007-1226
    DOI 10.1016/j.bjps.2023.02.002
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  3. Article: Biochar Obtained from

    Melo, André L F C / Carneiro, Marcelo T / Nascimento, Ariane M S S / Morais, Alan I S / Bezerra, Roosevelt D S / Viana, Bartolomeu C / Osajima, Josy A / Silva-Filho, Edson C

    Materials (Basel, Switzerland)

    2022  Volume 15, Issue 24

    Abstract: Given the increase in environmental pollution, especially of water, the emergence of studies that seek to develop strategies to mitigate/treat such effects have gained prominence in the world scientific community. Among the numerous adsorption processes, ...

    Abstract Given the increase in environmental pollution, especially of water, the emergence of studies that seek to develop strategies to mitigate/treat such effects have gained prominence in the world scientific community. Among the numerous adsorption processes, those made from biochar production stand out. This study analyzed the adsorption properties of the blue methylene model dye in the aqueous solution of biochar and activated biochar developed from pequi (
    Language English
    Publishing date 2022-12-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma15249076
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  4. Article: Pluripotency transcription factor levels in sheep embryos correlate with mRNA regulatory elements

    Nascimento, P. S. / Moura, M. T. / Oliveira-Silva, R. L. / Ramos-Deus, P. / Ferreira-Silva, J. C. / Filho, A. S. S. / Bartolomeu, C. C. / Benko-Iseppon, A. M. / Oliveira, M. A. L.

    Livestock science

    2022  Volume 255, Issue -, Page(s) 104778

    Language English
    Document type Article
    ZDB-ID 2226176-X
    ISSN 1871-1413
    Database Current Contents Nutrition, Environment, Agriculture

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  5. Article ; Online: Thromboelastography and Thromboelastometry in Assessment of Fibrinogen Deficiency and Prediction for Transfusion Requirement: A Descriptive Review.

    Peng, Henry T / Nascimento, Bartolomeu / Beckett, Andrew

    BioMed research international

    2018  Volume 2018, Page(s) 7020539

    Abstract: Fibrinogen is crucial for the formation of blood clot and clinical outcomes in major bleeding. Both Thromboelastography (TEG) and Rotational Thromboelastometry (ROTEM) have been increasingly used to diagnose fibrinogen deficiency and guide fibrinogen ... ...

    Abstract Fibrinogen is crucial for the formation of blood clot and clinical outcomes in major bleeding. Both Thromboelastography (TEG) and Rotational Thromboelastometry (ROTEM) have been increasingly used to diagnose fibrinogen deficiency and guide fibrinogen transfusion in trauma and surgical bleeding patients. We conducted a comprehensive and comparative review on the technologies and clinical applications of two typical functional fibrinogen assays using TEG (FF TEG) and ROTEM (FIBTEM) for assessment of fibrinogen level and deficiency, and prediction of transfusion requirement. Clot strength and firmness of FF TEG and ROTEM FIBTEM were the most used parameters, and their associations with fibrinogen levels as measured by Clauss method ranged from 0 to 0.9 for FF TEG and 0.27 to 0.94 for FIBTEM. A comparison of the interchangeability and clinical performance of the functional fibrinogen assays using the two systems showed that the results were correlated, but are not interchangeable between the two systems. It appears that ROTEM FIBTEM showed better associations with the Clauss method and more clinical use for monitoring fibrinogen deficiency and predicting transfusion requirements including fibrinogen replacement than FF TEG. TEG and ROTEM functional fibrinogen tests play important roles in the diagnosis of fibrinogen-related coagulopathy and guidance of transfusion requirements. Despite the fact that high-quality evidence is still needed, the two systems are likely to remain popular for the hemostatic management of bleeding patients.
    MeSH term(s) Afibrinogenemia/metabolism ; Afibrinogenemia/physiopathology ; Blood Coagulation Tests/methods ; Blood Transfusion/methods ; Fibrinogen/metabolism ; Humans ; Thrombelastography/methods ; Thrombosis/physiopathology
    Chemical Substances Fibrinogen (9001-32-5)
    Language English
    Publishing date 2018-11-25
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2018/7020539
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  6. Article ; Online: Blood transfusion and coagulopathy in geriatric trauma patients.

    Mador, Brett / Nascimento, Bartolomeu / Hollands, Simon / Rizoli, Sandro

    Scandinavian journal of trauma, resuscitation and emergency medicine

    2017  Volume 25, Issue 1, Page(s) 33

    Abstract: Background: Trauma resuscitation has undergone a paradigm shift with new emphasis on the early use of blood products and increased proportions of plasma and platelets. However, it is unclear how this strategy is applied or how effective it is in the ... ...

    Abstract Background: Trauma resuscitation has undergone a paradigm shift with new emphasis on the early use of blood products and increased proportions of plasma and platelets. However, it is unclear how this strategy is applied or how effective it is in the elderly population. The study aim is to identify differences in transfusion practices and the coagulopathy of trauma in the elderly.
    Methods: Data was prospectively collected on all consecutive patients that met trauma activation criteria at a Level I trauma centre. Data fields included patient demographics, co-morbidities, injury and resuscitation data, laboratory values, thromboelastography (TEG) results, and outcome measures. Elderly patients were defined as those 55 and older. Propensity-score matched analysis was completed for patients receiving blood product transfusion. Patients were matched by gender, mechanism, injury severity score (ISS), head injury, and time from injury.
    Results: Total of 628 patients were included, of which 142 (23%) were elderly. Elderly patients were more likely to be female (41% vs. 24%), suffer blunt mechanism of trauma (96% vs. 80%), have higher ISS scores (mean 25.4 vs. 21.6) and mortality (19% vs. 8%). Elderly patients were significantly more likely to receive a blood transfusion (42% vs. 30%), specifically for red cells and plasma. Propensity-matched analysis resulted in no difference in red cell transfusion or mortality. Despite the broad similarities between the matched cohorts, trauma coagulopathy as measured by TEG was less commonly observed in the elderly.
    Discussion: Our results suggest that elderly trauma patients are more likely to receive blood products when admitted to a trauma centre, though this may be attributed to under-triage. The results also suggest an altered coagulopathic response to traumatic injury which is partially influenced by increased anticoagulant and antiplatelet medication use in the geriatric population.
    Conclusion: It is not clear whether the acute coagulopathy of trauma is equivalent in geriatric patients, and further study is therefore warranted.
    MeSH term(s) Aged ; Blood Coagulation Disorders ; Blood Transfusion/methods ; Female ; Humans ; Male ; Middle Aged ; Practice Patterns, Physicians' ; Propensity Score ; Prospective Studies ; Trauma Centers ; Wounds and Injuries/blood
    Language English
    Publishing date 2017-03-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2455990-8
    ISSN 1757-7241 ; 1757-7241
    ISSN (online) 1757-7241
    ISSN 1757-7241
    DOI 10.1186/s13049-017-0374-0
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  7. Article: Pluripotency transcription factor levels in sheep embryos correlate with mRNA regulatory elements

    Nascimento, Pábola S. / Moura, Marcelo T. / Oliveira-Silva, Roberta L. / Ramos-Deus, Pamela / Ferreira-Silva, José C. / Filho, Antônio S. Santos / Bartolomeu, Cláudio C. / Benko-Iseppon, Ana M. / Oliveira, Marcos A.L.

    Livestock science. 2022 Jan., v. 255

    2022  

    Abstract: Pluripotency-associated transcription factors (PATF) contribute to cell fate decisions in embryos, cellular reprogramming, and the derivation of stem cells. PATF hold species-specific mRNA fluctuations during early development but the regulatory ... ...

    Abstract Pluripotency-associated transcription factors (PATF) contribute to cell fate decisions in embryos, cellular reprogramming, and the derivation of stem cells. PATF hold species-specific mRNA fluctuations during early development but the regulatory determinants remain largely unknown in sheep. The aim of this study was to prospect correlations between PATF gene expression patterns and mRNA regulatory features. The relative expression of PATF was investigated in sheep embryos by RT-qPCR and mRNAs were investigated for cis-regulatory elements and N6-methyladenosine (m6A) RNA methylation using bioinformatics. Four PATF gene expression patterns emerged with relatively distinct mRNA regulatory modes. RXRβ and TCF3 were detected in oocytes and mRNAs had variable 3′untranslated region (3′UTR) length. Three mRNAs (DPPA3, THAP11, and ZFX), which had similar abundance among developmental stages, frequently had short 3′UTRs and polyadelylation sites. NR5A2 and SP1 levels increased at the cleavage-stage but diminished in morulae, thus showing long 3′UTRs with multiple polyadelylation and m6A sites. Finally, mRNA abundance was low in oocytes and up-regulated in morulae (UTF1 and ZNF281) without a distinctive regulatory mode. Hence, sheep embryos hold PATF gene expression that correlates with mRNA 3′UTR length and the number of polyadenylation and m6A sites. These findings may lead to modulating sheep PATF to enhance reprogramming and stem cell derivation.
    Keywords bioinformatics ; early development ; gene expression ; methylation ; oocytes ; sheep ; stem cells ; transcription factors
    Language English
    Dates of publication 2022-01
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2226176-X
    ISSN 1878-0490 ; 1871-1413
    ISSN (online) 1878-0490
    ISSN 1871-1413
    DOI 10.1016/j.livsci.2021.104778
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Just another fascinating property of hypertonic solutions or something clinically relevant this time?

    Rizoli, Sandro B / Nascimento, Bartolomeu

    Critical care medicine

    2012  Volume 40, Issue 8, Page(s) 2519–2520

    MeSH term(s) Adenosine/therapeutic use ; Animals ; Blood Coagulation Disorders/drug therapy ; Drug Combinations ; Lidocaine/therapeutic use ; Magnesium/therapeutic use ; Male ; Shock, Hemorrhagic/drug therapy
    Chemical Substances Drug Combinations ; adenocaine ; Lidocaine (98PI200987) ; Magnesium (I38ZP9992A) ; Adenosine (K72T3FS567)
    Language English
    Publishing date 2012-08
    Publishing country United States
    Document type Comment ; Editorial
    ZDB-ID 197890-1
    ISSN 1530-0293 ; 0090-3493
    ISSN (online) 1530-0293
    ISSN 0090-3493
    DOI 10.1097/CCM.0b013e31825ae417
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    Zs.A 1261: Show issues Location:
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  9. Article ; Online: Housekeeping genes for RT-qPCR in ovine preimplantation embryos.

    Nascimento, Pábola Santos / Moura, Marcelo Tigre / Silva, Roberta Lane Oliveira / Ramos-Deus, Pamela / Ferreira-Silva, José Carlos / Veira, Joane Isis Travassos / Santos Filho, Antônio Santana / Guido, Sebastião Inocêncio / Bartolomeu, Cláudio Coutinho / Benko-Iseppon, Ana Maria / Oliveira, Marcos Antonio Lemos

    Zygote (Cambridge, England)

    2020  , Page(s) 1–8

    Abstract: Housekeeping genes (HKG) are paramount for accurate gene expression analysis during preimplantation development. Markedly, quantitative reverse transcription polymerase chain reaction (RT-qPCR) in ovine embryos currently lacks HKGs. Therefore, we tested ... ...

    Abstract Housekeeping genes (HKG) are paramount for accurate gene expression analysis during preimplantation development. Markedly, quantitative reverse transcription polymerase chain reaction (RT-qPCR) in ovine embryos currently lacks HKGs. Therefore, we tested 11 HKGs for RT-qPCR normalization during ovine parthenogenetic preimplantation development. Seven HKGs reached the qPCR efficiency threshold (97.20-105.96%), with correlation coefficients ranging from -0.922 to -0.998 and slopes from -3.22 to -3.59. GeNorm ranked glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) as the best HKG pair, while H3 histone, family 3A (H3F3A) was the third HKG. Relative gene expression was measured for zinc finger protein X-linked (ZFX) and developmental pluripotency-associated 3 (DPPA3) transcripts during ovine parthenogenetic preimplantation development. ZFX did not show any transcript abundance fluctuation among oocytes, cleavage-stage embryos, and morulae. DPPA3 transcript abundance was also similar among all developmental stages, therefore suggesting that it may not display a maternal gene expression profile. In silico analysis of ovine DPPA3 mRNA and protein showed high conservation to bovine orthologues. However, DPPA3 orthologues differed in regulatory motifs. In conclusion, GAPDH, TBP and H3F3A are stable HKGs in ovine parthenogenetic embryos and allow accurate RT-qPCR-based gene expression analysis.
    Language English
    Publishing date 2020-07-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 1166294-3
    ISSN 1469-8730 ; 0967-1994
    ISSN (online) 1469-8730
    ISSN 0967-1994
    DOI 10.1017/S0967199420000295
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  10. Article: Follicular size, luteinizing hormone(LH), and progesterone(P4) levels in postpartum Santa Ines ewes subjected to ram effect combined with suckling interruption

    Ferreira-Silva, J. C. / Filho, F. T. / Moura, M. T. / Nascimento, R. S. / Oliveira, L. R. S. / Bartolomeu, C. C. / Oliveira, M. A. L.

    Livestock science

    2018  Volume 214, Issue -, Page(s) 88

    Language English
    Document type Article
    ZDB-ID 2226176-X
    ISSN 1871-1413
    Database Current Contents Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

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