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Article: The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation.

Yu, Wei / Li, Qi / Shao, Changlei / Zhang, Yijia / Kang, Cai / Zheng, Yang / Liu, Xihao / Liu, Xincheng / Yan, Jing

2022 Volume 13, Page(s) 946065

Abstract: ... Xiang-Wei-Kang (CW) formula has been utilized to treat gastrointestinal disorders in the clinic ...

Abstract	Inflammatory bowel disease (IBD) is pathologically characterized by an immune response accommodative insufficiency and dysbiosis accompanied by persistent epithelial barrier dysfunction. The Cao-Xiang-Wei-Kang (CW) formula has been utilized to treat gastrointestinal disorders in the clinic. The present study was designed to delineate the pharmacological mechanisms of this formula from different aspects of the etiology of ulcerative colitis (UC), a major subtype of IBD. Dextran sodium sulfate (DSS) was given to mice for a week at a concentration of 2%, and the CW solution was administered for 3 weeks. 16S rRNA gene sequencing and untargeted metabolomics were conducted to examine the changes in the microbiome profile, and biochemical experiments were performed to confirm the therapeutic functions predicted by system pharmacology analysis. The CW treatment hampered DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis, which was corroborated by suppressed caspase 3 (Casp3) and interleukin-1b (IL-1b) and increased cleaved caspase 3 expression and casp-3 activity in the colon samples from colitis mice subjected to the CW therapy. Moreover, the CW therapy rescued the decreased richness and diversity, suppressed the potentially pathogenic phenotype of the gut microorganisms, and reversed the altered linoleic acid metabolism and cytochrome P450 activity in murine colitis models. In our
Language	English
Publishing date	2022-09-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.946065
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Article: Exploring the therapeutic effect of Pen Yan Kang Fu Decoction on SPID rats based on LIF/JAK2/STAT3 signaling pathway.

Ji, Xiaoli / Hu, Quan / Yang, Chengcheng / Huang, Li / Huang, Yefang / Deng, Linwen / Song, Xiaoqing / Zhang, Yongqing / Wang, Yan

3 Biotech

2024 Volume 14, Issue 5, Page(s) 134

Abstract: ... problem in clinical research. The efficacy of Pen Yan Kang Fu Decoction (PYKFD) has been verified in long ...

Abstract	Tubal inflammation, endometritis, and uterine adhesions due to post-pelvic inflammatory disease (SPID) are important causes of infertility. Chronic endometritis (CE) belongs to SPID, which seriously affects women's reproductive health, quality of life, and family harmony, and is a hot and difficult problem in clinical research. The efficacy of Pen Yan Kang Fu Decoction (PYKFD) has been verified in long-term clinical practice for chronic endometritis infertility caused by the SPID. Numerous studies have confirmed that the LIF/JAK2/STAT3 signaling pathway is important in embryo implantation and development, and endometritis infertility is close to LIF/JAK2/STAT3. In vivo results showed that PYKFD increased endometrial receptivity, repaired uterine tissue damage, and regulates the expression of endometrial receptivity-related factors ER (estrogen receptor), PR (progesterone receptor), CD31, and integrin αvβ3, and induced the transduction of LIF/JAK2/STAT3 signaling pathway. PYKFD can also regulate the expression of IL-6. The results of in vitro experiments showed that PYKFD regulates the behavior of rat endometrial epithelial cells (REECs) involving LIF. In conclusion, PYKFD can improve endometrial receptivity and promote endometrial repair by LIF/JAK2/STAT3 signaling pathway. Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-03981-0.
Language	English
Publishing date	2024-04-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-024-03981-0
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Article ; Online: Chang-Kang-Fang alleviates diarrhea predominant irritable bowel syndrome (IBS-D) through inhibiting TLR4/NF-κB/NLRP3 pathway.

Zhang, Sihao / Tian, Danmei / Xia, Zixuan / Yang, Fengge / Chen, Yanhui / Yao, Zhihong / He, Yi / Miao, Xinglong / Zhou, Guirong / Yao, Xinsheng / Tang, Jinshan

Journal of ethnopharmacology

2024 Volume 330, Page(s) 118236

Abstract: Ethnopharmacological relevance: Chang-Kang-Fang (CKF), originated ...

Abstract	Ethnopharmacological relevance: Chang-Kang-Fang (CKF), originated from traditional Chinese medicine (TCM) formulas, has been utilized to treat diarrhea predominant irritable bowel syndrome (IBS-D) based on clinical experience. However, the underlying mechanism of CKF for treating IBS-D remains unclear and need further clarification. Aim of the study: The objective of this present investigation was to validate the efficacy of CKF on IBS-D model rats and to uncover its potential mechanism for the treatment of IBS-D. Materials and methods: We first established the IBS-D rat model through neonatal maternal separation (NMS) in combination with restraint stress (RS) and the administration of senna decoction via gavage. To confirm the therapeutic effect of CKF on treating IBS-D, abdominal withdrawal reflex (AWR) scores, the quantity of fecal pellets, and the fecal water content (FWC) were measured to evaluate the influence of CKF on visceral hypersensitivity and the severity of diarrhea symptom after the intragastric administration of CKF for 14 days. Subsequently, enzyme linked immunosorbent assay (ELISA) was applied to assess the effect of CKF on neuropeptides substance P (SP) and 5-hydroxytryptamine (5-HT), as well as inflammatory cytokines in serum and in intestinal tissues. Further, colonic pathological changes, the amount of colonic mast cells, and the expression level of occludin in rat colon tissues, were investigated by hematoxylin-eosin (HE) staining, toluidine blue staining, and immunohistochemistry, respectively. To explore the underlying mechanisms, alterations in colonic RNA transcriptomics for the normal, model, and CKF treatment groups were assessed using RNA sequencing (RNA-Seq). Subsequently, quantitative real-time polymerase chain reaction (qRT-PCR), Western blot (WB), and immunofluorescence (IF) assays were applied to validate the effect of CKF on predicted pathways in vivo and in vitro. In addition, to elucidate the potential active compounds in CKF, 11 representative components found in CKF were selected, and their anti-inflammation potentials were evaluated using LPS-treated RAW264.7 cell models. Results: CKF treatment significantly reduced the number of fecal pellets, attenuated visceral hypersensitivity, and decreased 5-HT and SP concentrations in serum and colon tissues, along with a reduction in colonic mast cell counts, correlating with improved symptoms in IBS-D rats. Meanwhile, CKF treatment reduced the colonic inflammatory cell infiltration, lowered the levels of IL-6, TNF-α, and IL-1β in serum and colon tissues, and increased the occludin protein expression in colon tissues to improve inflammatory response and colonic barrier function. RNA-Seq, in conjugation with our previous network pharmacology analysis, indicated that CKF might mitigate the symptoms of IBS-D rats by inhibiting the Toll like receptor 4/Nuclear factor kappa-B/NLR family pyrin domain-containing protein 3 (TLR4/NF-κB/NLRP3) pathway, which was confirmed by WB, IF, and qRT-PCR experiments in vivo and in vitro. Furthermore, coptisine, berberine, hyperoside, epicatechin, and gallic acid present in CKF emerged as potential active components for treating IBS-D, as they demonstrated in vitro anti-inflammatory effects. Conclusion: Our findings demonstrate that CKF effectively improves the symptoms of IBS-D rats, potentially through the inhibition of the TLR4/NF-κB/NLRP3 pathway. Moreover, this study unveils the potential bioactive components in CKF that could be applied in the treatment of IBS-D.
Language	English
Publishing date	2024-04-24
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2024.118236
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Article: Uncovering the mechanism of Kang-ai injection for treating intrahepatic cholangiocarcinoma based on network pharmacology, molecular docking, and

Song, Fei / Lu, Chang-Liang / Wang, Cheng-Gui / Hu, Chen-Wei / Zhang, Yu / Wang, Tian-Lun / Han, Lu / Chen, Zhong

Frontiers in pharmacology

2023 Volume 14, Page(s) 1129709

Abstract: Objective: ...

Abstract	Objective:
Language	English
Publishing date	2023-03-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1129709
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Article ; Online: Studies on chemical profiling and pharmacokinetics of traditional Chinese medicine Formula Kang Shuai Lao Pian

Chengjuan Liu / Qibao Jiang / Zhirong Zhou / Peng Lei / Peng Zhang / Xin Chai / Guixiang Pan / Yuefei Wang / Miaomiao Jiang

Arabian Journal of Chemistry, Vol 17, Iss 1, Pp 105398- (2024)

1481

Abstract: Kang Shuai Lao Pian (KSLP) is a traditional Chinese medicine (TCM) preparation used to delay aging ...

Abstract	Kang Shuai Lao Pian (KSLP) is a traditional Chinese medicine (TCM) preparation used to delay aging. However, due to the lack of research on the chemical composition and pharmacokinetic behavior of KSLP, its material basis and in vivo components with high exposure remain ambiguous. The UPLC/Q-Orbitrap-MS/MS was performed to identify the preliminary chemical profile of KSLP. A total of 138 compounds, including ginsenosides, phenylethanol glycosides, iridoids, alkaloids, ionones and others, were identified in accordance with their retention times, accurate masses and characteristic MS/MS fragment patterns. Moreover, considering the active components and characteristic components of KSLP, the extraction process of KSLP was optimized, and the quantitative analysis by UPLC/QQQ-MS/MS of 13 compounds in KSLP was established. The method was stable and sensitive, and could be used for the quality control of KSLP. Then, the pharmacokinetic study was carried out by further refining the components of KSLP. Besides, quantitative method for 6 compounds in rat plasma was validated and developed by UPLC/QQQ-MS/MS. The established approach was successfully applied to characterize the pharmacokinetic features of components in KSLP and it was found that the absorption and elimination of ginsenosides in KSLP was slow. Altogether, this study laid a solid foundation and provided theoretical guidance for further clarification of bioactive components of KSLP.
Keywords	Kang Shuai Lao Pian ; UPLC/Q-Orbitrap-MS/MS ; UPLC/QQQ-MS/MS ; Qualitative analysis ; Quantitative analysis ; Pharmacokinetics analysis ; Chemistry ; QD1-999
Subject code	540
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Shen-Kang protects against tacrolimus-induced renal injury.

Zhang, Long Ye / Jin, Jian / Luo, Kang / Piao, Shang Guo / Zheng, Hai Lan / Jin, Ji Zhe / Lim, Sun Woo / Choi, Bum Soon / Yang, Chul Woo / Li, Can

The Korean journal of internal medicine

2018 Volume 34, Issue 5, Page(s) 1078–1090

Abstract: Background/aims: Evidence suggests that Shen-Kang (SK), a traditional Chinese herbal medicine ...

Abstract	Background/aims: Evidence suggests that Shen-Kang (SK), a traditional Chinese herbal medicine, protects against various types of renal injury. In this study, we evaluated whether SK treatment confers renoprotection in a rat model of chronic tacrolimus (TAC) nephropathy. Methods: Rats were treated daily with TAC (1.5mg/kg, subcutaneously) and SK (450 mg/kg, intravenously) for 4 weeks. The effects of SK on TAC-induced renal injury were assessed by measuring renal function, urine albumin excretion, histopathology, inflammatory cell infiltration, expression of profibrotic (transforming growth factor β1 [TGF-β1] and TGF-β inducible gene-h3 [βig-h3]) and proinflammatory cytokines, oxidative stress, and apoptotic cell death. Results: Administration of SK preserved glomerular integrity (fractional mesangial area and Wilms tumor 1-positive glomeruli), attenuated tubulointerstitial fibrosis, and reduced the number of ectodermal dysplasia 1-positive cells, and this was paralleled by improved urine albumin excretion and renal dysfunction. At the molecular level, SK treatment suppressed expression of TGF-β1/Smad2/3, βig-h3, and proinflammatory cytokines. Oxidative stress and apoptotic cell death were significantly decreased with SK treatment, and apoptosis-related genes were regulated toward cell survival (active caspase-3 and the B-cell lymphoma-2/Bcl2-associated X [Bcl-2/Bax] ratio). Conclusion: SK protects against TAC-induced renal injury.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Apoptosis Regulatory Proteins/metabolism ; Cytokines/metabolism ; Cytoprotection ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Extracellular Matrix Proteins/metabolism ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Kidney Diseases/chemically induced ; Kidney Diseases/metabolism ; Kidney Diseases/pathology ; Kidney Diseases/prevention & control ; Male ; Oxidative Stress/drug effects ; Protective Agents/pharmacology ; Rats, Sprague-Dawley ; Signal Transduction ; Tacrolimus ; Transforming Growth Factor beta/metabolism ; Transforming Growth Factor beta1/metabolism
Chemical Substances	Apoptosis Regulatory Proteins ; Cytokines ; Drugs, Chinese Herbal ; Extracellular Matrix Proteins ; Protective Agents ; Tgfb1 protein, rat ; Transforming Growth Factor beta ; Transforming Growth Factor beta1 ; shenkang ; betaIG-H3 protein (148710-76-3) ; Tacrolimus (WM0HAQ4WNM)
Language	English
Publishing date	2018-02-12
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	639023-7
ISSN	2005-6648 ; 1226-3303
ISSN (online)	2005-6648
ISSN	1226-3303
DOI	10.3904/kjim.2017.276
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Article: Evaluation of the effectiveness and mechanism of action of the Chang-Kang-Fang formula combined with bifid triple viable capsules on diarrhea-predominant irritable bowel syndrome.

Sun, Jing / Zhang, Mengqiu / Liu, Wei / Liu, Youqian / Zhang, Dongjian / Fan, Xinyu / Zhang, Jian / Li, Tian / Lu, Min

Frontiers in microbiology

2023 Volume 14, Page(s) 1160783

Abstract: Introduction: The Chang-Kang-Fang (CKF) formula, a traditional Chinese herbal formula ...

Abstract	Introduction: The Chang-Kang-Fang (CKF) formula, a traditional Chinese herbal formula, can decrease serotonin (5-HT) levels and treat irritable bowel syndrome (IBS). Probiotics have a better synergistic effect on diarrhea-predominant IBS (IBS-D) when combined with 5-HT Methods: The rat models of IBS-D were induced by gavage with senna decoction plus restraint stress. The CKF formula, PFK and their combination were administered to the rats. Their effects were evaluated based on general condition of the rats and the AWR score. The levels of 5-HT and fos protein in the colon and hippocampus were measured by immunohistochemistry. The levels of SP and VIP, as well as ZO-1 and occludin in the colon, were determined by enzyme-linked immunosorbent assay and immunohistochemistry. The intestinal microbiota in faeces was analyzed by 16S rRNA high-throughput sequencing. Results: The results showed that the oral CKF formula combined with PFK (CKF + PFK) could significantly relieve the symptoms of IBS-D, including elevating the weight rate and decreasing the AWR score. Compared with the MC group, administration of CKF + PFK significantly reduced the expression of fos in the colon and hippocampus and that of 5-HT, SP and VIP in the colon and increased the levels of 5-HT in the hippocampus and ZO-1 and occludin in the colon. The above indexes exhibited statistical significance in the CKF + PFK group relative to those in the other groups. Moreover, treatment with CKF + PFK improved the diversity of intestinal microbiota and the abundance of Conclusions: The CKF formula combined with PFK may have a synergistic effect on IBS-D by slowing gastrointestinal motility, lowering visceral hypersensitivity, enhancing the intestinal barrier function and modulating the composition of intestinal microbiota.
Language	English
Publishing date	2023-06-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1160783
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Article ; Online: Deciphering chemical and metabolite profiling of Chang-Kang-Fang by UPLC-Q-TOF-MS/MS and its potential active components identification.

Yang, Fengge / Zhang, Sihao / Tian, Danmei / Zhou, Guirong / Tang, Xiyang / Miao, Xinglong / He, Yi / Yao, Xinsheng / Tang, Jinshan

Chinese journal of natural medicines

2023 Volume 21, Issue 6, Page(s) 459–480

Abstract: Chang-Kang-Fang (CKF) formula, a Traditional Chinese Medicine (TCM) prescription, has been widely ...

Abstract	Chang-Kang-Fang (CKF) formula, a Traditional Chinese Medicine (TCM) prescription, has been widely used for the treatment of irritable bowel syndrome (IBS). However, its potential material basis and underlying mechanism remain elusive. Therefore, this study employed an integrated approach that combined ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) with network pharmacology to systematically characterize the phytochemical components and metabolites of CKF, as well as elucidating its underlying mechanism. Through this comprehensive analysis, a total of 150 components were identified or tentatively characterized within the CKF formula. Notably, six N-acetyldopamine oligomers from CicadaePeriostracum and eight resin glycosides from Cuscutae Semen were characterized in this formula for the first time. Meanwhile, 149 xenobiotics (58 prototypes and 91 metabolites) were detected in plasma, urine, feces, brain, and intestinal contents, and the in vivo metabolic pathways of resin glycosides were elaborated for the first time. Furthermore, network pharmacology and molecular docking analyses revealed that alkaloids, flavonoids, chromones, monoterpenes, N-acetyldopamine dimers, p-hydroxycinnamic acid, and Cus-3/isomer might be responsible for the beneficial effects of CKF in treating IBS, and CASP8, MARK14, PIK3C, PIK3R1, TLR4, and TNF may be its potential targets. These discoveries offer a comprehensive understanding of the potential material basis and clarify the underlying mechanism of the CKF formula in treating IBS, facilitating the broader application of CKF in the field of medicine.
MeSH term(s)	Humans ; Tandem Mass Spectrometry/methods ; Irritable Bowel Syndrome/drug therapy ; Molecular Docking Simulation ; Drugs, Chinese Herbal/chemistry ; Glycosides ; Chromatography, High Pressure Liquid/methods
Chemical Substances	chang-kang-fang ; N-acetyldopamine (2494-12-4) ; Drugs, Chinese Herbal ; Glycosides
Language	English
Publishing date	2023-06-12
Publishing country	China
Document type	Journal Article
ZDB-ID	2192577-X
ISSN	1875-5364 ; 2095-6975 ; 1672-3651
ISSN (online)	1875-5364
ISSN	2095-6975 ; 1672-3651
DOI	10.1016/S1875-5364(23)60474-1
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Zs.A 6113: Show issues

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Article ; Online: Molecular Mechanisms Underlying the Effects of Bimin Kang Mixture on Allergic Rhinitis: Network Pharmacology and RNA Sequencing Analysis.

Qi, Li-Jie / Wang, Ren-Zhong / Gao, Shang / Chen, Xiang-Jing / Zhang, Xin / Zhang, Yi-Peng

BioMed research international

2022 Volume 2022, Page(s) 7034078

Abstract: ... disease of the respiratory tract. Previous studies have demonstrated that Bimin Kang Mixture (BMK) is effective in alleviating AR ...

Abstract	Background: Allergic rhinitis (AR) is a highly prevalent chronic inflammatory disease of the respiratory tract. Previous studies have demonstrated that Bimin Kang Mixture (BMK) is effective in alleviating AR symptoms and reducing the secretion of inflammatory factors and mucin; however, the precise mechanisms underlying these effects remain unclear. Methods: We built target networks for each medication component using a network pharmacology technique and used RNA-seq transcriptome analysis to screen differentially expressed genes (DEGs) for AR patients and control groups. The overlapping targets in the two groups were assessed using PPI networks, GO, and KEGG enrichment analyses. The binding ability of essential components to dock with hub target genes was investigated using molecular docking. Finally, we demonstrate how BMK can treat AR by regulating the NF- Results: Effective targets from network pharmacology were combined with DEGs from RNA-seq, with 20 intersections as key target genes. The construction of the PPI network finally identified 5 hub target genes, and all hub target genes were in the NF- Conclusion: BMK by regulating the NF-
MeSH term(s)	Animals ; NF-kappa B/genetics ; NF-kappa B/metabolism ; Molecular Docking Simulation ; Network Pharmacology ; Rhinitis, Allergic/drug therapy ; Rhinitis, Allergic/genetics ; Sequence Analysis, RNA ; Drugs, Chinese Herbal
Chemical Substances	NF-kappa B ; Drugs, Chinese Herbal
Language	English
Publishing date	2022-10-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2022/7034078
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Article ; Online: Molecular Mechanisms Underlying the Effects of Bimin Kang Mixture on Allergic Rhinitis

Li-Jie Qi / Ren-Zhong Wang / Shang Gao / Xiang-Jing Chen / Xin Zhang / Yi-Peng Zhang

BioMed Research International, Vol

Network Pharmacology and RNA Sequencing Analysis

2022 Volume 2022

Abstract: ... disease of the respiratory tract. Previous studies have demonstrated that Bimin Kang Mixture (BMK) is effective in alleviating AR ...

Abstract	Background. Allergic rhinitis (AR) is a highly prevalent chronic inflammatory disease of the respiratory tract. Previous studies have demonstrated that Bimin Kang Mixture (BMK) is effective in alleviating AR symptoms and reducing the secretion of inflammatory factors and mucin; however, the precise mechanisms underlying these effects remain unclear. Methods. We built target networks for each medication component using a network pharmacology technique and used RNA-seq transcriptome analysis to screen differentially expressed genes (DEGs) for AR patients and control groups. The overlapping targets in the two groups were assessed using PPI networks, GO, and KEGG enrichment analyses. The binding ability of essential components to dock with hub target genes was investigated using molecular docking. Finally, we demonstrate how BMK can treat AR by regulating the NF-κB signaling pathway through animal experiments. Results. Effective targets from network pharmacology were combined with DEGs from RNA-seq, with 20 intersections as key target genes. The construction of the PPI network finally identified 5 hub target genes, and all hub target genes were in the NF-κB signaling pathway. Molecular docking suggests that citric acid, deoxyandrographolide, quercetin, luteolin, and kaempferol are structurally stable and can spontaneously attach to IL-1β, CXCL2, CXCL8, CCL20, and PTGS2 receptors. Animal experiments have shown that BMK inhibits NF-κB transcription factor activation, reduces the expression of proinflammatory cytokines and chemokines IL-1β, CXCL2, IL-8, and COX-2, and exerts anti-inflammatory and anti-allergic effects. Conclusion. BMK by regulating the NF-κB signaling pathway improves inflammatory cell infiltration, regulates mucosal immune balance, and reduces airway hypersensitivity. These findings provide theoretical support for the clinical efficacy of BMK for AR treatment.
Keywords	Medicine ; R
Subject code	570
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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