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  1. Article: Cortical microtubules oppose actomyosin-driven membrane ingression during

    Quiogue, Alyssa R / Sumiyoshi, Eisuke / Fries, Adam / Chuang, Chien-Hui / Bowerman, Bruce

    bioRxiv : the preprint server for biology

    2023  

    Abstract: ... ...

    Abstract During
    Language English
    Publishing date 2023-05-26
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.26.542508
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: C. elegans XMAP215/ZYG-9 and TACC/TAC-1 act at multiple times during oocyte meiotic spindle assembly and promote both spindle pole coalescence and stability.

    Harvey, Austin M / Chuang, Chien-Hui / Sumiyoshi, Eisuke / Bowerman, Bruce

    PLoS genetics

    2023  Volume 19, Issue 1, Page(s) e1010363

    Abstract: The conserved two-component XMAP215/TACC modulator of microtubule stability is required in multiple animal phyla for acentrosomal spindle assembly during oocyte meiotic cell division. In C. elegans, XMAP215/zyg-9 and TACC/tac-1 mutant oocytes exhibit ... ...

    Abstract The conserved two-component XMAP215/TACC modulator of microtubule stability is required in multiple animal phyla for acentrosomal spindle assembly during oocyte meiotic cell division. In C. elegans, XMAP215/zyg-9 and TACC/tac-1 mutant oocytes exhibit multiple and indistinguishable oocyte spindle assembly defects beginning early in meiosis I. To determine if these defects represent one or more early requirements with additional later and indirect consequences, or multiple temporally distinct and more direct requirements, we have used live cell imaging and fast-acting temperature-sensitive zyg-9 and tac-1 alleles to dissect their requirements at high temporal resolution. Temperature upshift and downshift experiments indicate that the ZYG-9/TAC-1 complex has multiple temporally distinct and separable requirements throughout oocyte meiotic cell division. First, we show that during prometaphase ZYG-9 and TAC-1 promote the coalescence of early pole foci into a bipolar structure, stabilizing pole foci as they grow and limiting their growth rate, with these requirements being independent of an earlier defect in microtubule organization that occurs upon nuclear envelope breakdown. Second, during metaphase, ZYG-9 and TAC-1 maintain spindle bipolarity by suppressing ectopic pole formation. Third, we show that ZYG-9 and TAC-1 also are required for spindle assembly during meiosis II, independently of their meiosis I requirements. The metaphase pole stability requirement appears to be important for maintaining chromosome congression, and we discuss how negative regulation of microtubule stability by ZYG-9/TAC-1 during oocyte meiotic cell division might account for the observed defects in spindle pole coalescence and stability.
    MeSH term(s) Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Spindle Apparatus/metabolism ; Microtubules/genetics ; Microtubules/metabolism ; Spindle Poles/genetics ; Meiosis/genetics ; Oocytes/metabolism
    Language English
    Publishing date 2023-01-06
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1010363
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Microtubules oppose cortical actomyosin-driven membrane ingression during C. elegans meiosis I polar body extrusion.

    Quiogue, Alyssa R / Sumiyoshi, Eisuke / Fries, Adam / Chuang, Chien-Hui / Bowerman, Bruce

    PLoS genetics

    2023  Volume 19, Issue 10, Page(s) e1010984

    Abstract: During C. elegans oocyte meiosis I cytokinesis and polar body extrusion, cortical actomyosin is locally remodeled to assemble a contractile ring that forms within and remains part of a much larger and actively contractile cortical actomyosin network. ... ...

    Abstract During C. elegans oocyte meiosis I cytokinesis and polar body extrusion, cortical actomyosin is locally remodeled to assemble a contractile ring that forms within and remains part of a much larger and actively contractile cortical actomyosin network. This network both mediates contractile ring dynamics and generates shallow ingressions throughout the oocyte cortex during polar body extrusion. Based on our analysis of requirements for CLS-2, a member of the CLASP family of proteins that stabilize microtubules, we recently proposed that a balance of actomyosin-mediated tension and microtubule-mediated stiffness limits membrane ingression throughout the oocyte during meiosis I polar body extrusion. Here, using live cell imaging and fluorescent protein fusions, we show that CLS-2 is part of a group of kinetochore proteins, including the scaffold KNL-1 and the kinase BUB-1, that also co-localize during meiosis I to structures called linear elements, which are present within the assembling oocyte spindle and also are distributed throughout the oocyte in proximity to, but appearing to underlie, the actomyosin cortex. We further show that KNL-1 and BUB-1, like CLS-2, promote the proper organization of sub-cortical microtubules and also limit membrane ingression throughout the oocyte. Moreover, nocodazole or taxol treatment to destabilize or stabilize oocyte microtubules leads to, respectively, excess or decreased membrane ingression throughout the oocyte. Furthermore, taxol treatment, and genetic backgrounds that elevate the levels of cortically associated microtubules, both suppress excess membrane ingression in cls-2 mutant oocytes. We propose that linear elements influence the organization of sub-cortical microtubules to generate a stiffness that limits cortical actomyosin-driven membrane ingression throughout the oocyte during meiosis I polar body extrusion. We discuss the possibility that this regulation of sub-cortical microtubule dynamics facilitates actomyosin contractile ring dynamics during C. elegans oocyte meiosis I cell division.
    MeSH term(s) Animals ; Actomyosin/genetics ; Actomyosin/metabolism ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Polar Bodies ; Cytokinesis/genetics ; Spindle Apparatus/genetics ; Spindle Apparatus/metabolism ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Microtubules/genetics ; Microtubules/metabolism ; Meiosis/genetics ; Oocytes/metabolism ; Paclitaxel ; Microtubule-Associated Proteins/genetics
    Chemical Substances Actomyosin (9013-26-7) ; Caenorhabditis elegans Proteins ; Paclitaxel (P88XT4IS4D) ; KNL-1 protein, C elegans ; Microtubule-Associated Proteins
    Language English
    Publishing date 2023-10-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1010984
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Microtubule assembly and pole coalescence

    Chien-Hui Chuang / Aleesa J. Schlientz / Jie Yang / Bruce Bowerman

    Biology Open, Vol 9, Iss

    early steps in Caenorhabditis elegans oocyte meiosis I spindle assembly

    2020  Volume 6

    Abstract: How oocytes assemble bipolar meiotic spindles in the absence of centrosomes as microtubule organizing centers remains poorly understood. We have used live cell imaging in Caenorhabditis elegans to investigate requirements for the nuclear lamina and for ... ...

    Abstract How oocytes assemble bipolar meiotic spindles in the absence of centrosomes as microtubule organizing centers remains poorly understood. We have used live cell imaging in Caenorhabditis elegans to investigate requirements for the nuclear lamina and for conserved regulators of microtubule dynamics during oocyte meiosis I spindle assembly, assessing these requirements with respect to recently identified spindle assembly steps. We show that the nuclear lamina is required for microtubule bundles to form a peripheral cage-like structure that appears shortly after oocyte nuclear envelope breakdown and surrounds the oocyte chromosomes, although bipolar spindles still assembled in its absence. Although two conserved regulators of microtubule nucleation, RAN-1 and γ-tubulin, are not required for bipolar spindle assembly, both contribute to normal levels of spindle-associated microtubules and spindle assembly dynamics. Finally, the XMAP215 ortholog ZYG-9 and the nearly identical minus-end directed kinesins KLP-15/16 are required for proper assembly of the early cage-like structure of microtubule bundles, and for early spindle pole foci to coalesce into a bipolar structure. Our results provide a framework for assigning molecular mechanisms to recently described steps in C. elegans oocyte meiosis I spindle assembly.
    Keywords meiotic spindle assembly ; microtubules ; oocyte meiosis ; Science ; Q ; Biology (General) ; QH301-705.5
    Subject code 670
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher The Company of Biologists
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Microtubule assembly and pole coalescence: early steps in

    Chuang, Chien-Hui / Schlientz, Aleesa J / Yang, Jie / Bowerman, Bruce

    Biology open

    2020  Volume 9, Issue 6

    Abstract: How oocytes assemble bipolar meiotic spindles in the absence of centrosomes as microtubule organizing centers remains poorly understood. We have used live cell imaging ... ...

    Abstract How oocytes assemble bipolar meiotic spindles in the absence of centrosomes as microtubule organizing centers remains poorly understood. We have used live cell imaging in
    MeSH term(s) Animals ; Caenorhabditis elegans/physiology ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Fluorescent Antibody Technique ; Gene Knockdown Techniques ; Meiosis ; Microtubule-Organizing Center ; Microtubules/metabolism ; Oocytes/physiology ; Spindle Apparatus/metabolism ; Spindle Poles/metabolism ; ran GTP-Binding Protein
    Chemical Substances Caenorhabditis elegans Proteins ; RAN-1 protein, C elegans (EC 3.6.5.2) ; ran GTP-Binding Protein (EC 3.6.5.2)
    Language English
    Publishing date 2020-06-25
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2632264-X
    ISSN 2046-6390
    ISSN 2046-6390
    DOI 10.1242/bio.052308
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Subacute thyroiditis in a patient with psoriasis treated with a tumor necrosis factor-α inhibitor.

    Wei, Yu-An / Chuang, Wan-Chi / Hong, Chien-Hui

    International journal of dermatology

    2018  Volume 57, Issue 7, Page(s) 869–871

    MeSH term(s) Adalimumab/adverse effects ; Adult ; Anti-Inflammatory Agents/adverse effects ; Arthritis, Psoriatic/drug therapy ; Female ; Humans ; Thyroiditis, Subacute/chemically induced ; Tumor Necrosis Factor-alpha/antagonists & inhibitors
    Chemical Substances Anti-Inflammatory Agents ; Tumor Necrosis Factor-alpha ; Adalimumab (FYS6T7F842)
    Language English
    Publishing date 2018-02-20
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 412254-9
    ISSN 1365-4632 ; 0011-9059 ; 1461-1244
    ISSN (online) 1365-4632
    ISSN 0011-9059 ; 1461-1244
    DOI 10.1111/ijd.13941
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: A Phase II Study of Neoadjuvant Chemoradiotherapy with Docetaxel, Cisplatin and 5-FU Followed by Surgical Resection in the Treatment of Locally Advanced Esophagogastric Junction Cancer and Locally Advanced Esophageal Cancer.

    Chen, Chien-Chih / Yeh, Hui-Ling / Chuang, Cheng-Yeh / Hsu, Chung-Ping

    Clinics and practice

    2024  Volume 14, Issue 2, Page(s) 642–652

    Abstract: Purpose: We conducted a phase II study evaluating chemoradiotherapy in patients with advanced esophageal cancer, using the docetaxel, cisplatin, and 5-fluorouracil (DCF) regimen followed by surgery. The primary purposes of this clinical trial were to ... ...

    Abstract Purpose: We conducted a phase II study evaluating chemoradiotherapy in patients with advanced esophageal cancer, using the docetaxel, cisplatin, and 5-fluorouracil (DCF) regimen followed by surgery. The primary purposes of this clinical trial were to assess the efficacy and safety of chemoradiotherapy employing the DCF regimen in the treatment of advanced esophageal cancer.
    Material and methods: We enrolled a total of 24 newly diagnosed esophageal cancer patients between April 2015 and November 2017 in this prospective study. The radiotherapy regimen consisted of a total dose of 45 Gy in 25 fractions. The chemotherapy protocol included docetaxel 35 mg/m
    Results: The study population had a median age of 55.5 years, ranging from 44 to 66, with over 90% of the patients being male. The 5-year DFS was 37.1%, and the 5-year OS was 48.7%. The pathologic complete response rate was 45.8% (11/24). The most common types of toxicity were leukopenia and thrombocytopenia. No grade 3 or greater hematologic toxicity was reported.
    Conclusions: The use of the DCF regimen in neoadjuvant chemoradiotherapy followed by surgery demonstrated tolerable toxicity and achieved acceptable DFS and OS outcomes.
    Language English
    Publishing date 2024-04-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2605724-4
    ISSN 2039-7283 ; 2039-7275
    ISSN (online) 2039-7283
    ISSN 2039-7275
    DOI 10.3390/clinpract14020051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Rapid Trio Exome Sequencing for Autosomal Recessive Renal Tubular Dysgenesis in Recurrent Oligohydramnios.

    Lin, Shin-Yu / Chuang, Gwo-Tsann / Hung, Chien-Hui / Lin, Wei-Chou / Jeng, Yung-Ming / Yen, Ting-An / Chang, Karine / Chien, Yin-Hsiu / Hwu, Wuh-Liang / Lee, Chien-Nan / Tsai, I-Jung / Lee, Ni-Chung

    Frontiers in genetics

    2021  Volume 12, Page(s) 606970

    Abstract: Oligohydramnios is not a rare prenatal finding. However, recurrent oligohydramnios is uncommon, and genetic etiology should be taken into consideration. We present two families with recurrent fetal oligohydramnios that did not respond to amnioinfusion. ... ...

    Abstract Oligohydramnios is not a rare prenatal finding. However, recurrent oligohydramnios is uncommon, and genetic etiology should be taken into consideration. We present two families with recurrent fetal oligohydramnios that did not respond to amnioinfusion. Rapid trio-whole-exome sequencing (WES) revealed mutations in the
    Language English
    Publishing date 2021-06-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2021.606970
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Trends and hotspots related to traditional and modern approaches on acupuncture for stroke: A bibliometric and visualization analysis.

    Chuang, Chao-Yu / Chou, Willy / Chien, Tsair-Wei / Jen, Tung-Hui

    Medicine

    2023  Volume 102, Issue 48, Page(s) e35332

    Abstract: Background: Acupuncture role in stroke treatment and post-stroke rehabilitation has garnered significant attention. However, there is a noticeable gap in bibliometric studies on this topic. Additionally, the precision and comprehensive methodology of ... ...

    Abstract Background: Acupuncture role in stroke treatment and post-stroke rehabilitation has garnered significant attention. However, there is a noticeable gap in bibliometric studies on this topic. Additionally, the precision and comprehensive methodology of cluster analysis remain underexplored. This research sought to introduce an innovative cluster analysis technique (called follower-leading clustering algorithm, FLCA) to evaluate global publications and trends related to acupuncture for stroke in the recent decade.
    Methods: Publications pertaining to acupuncture for stroke from 2013 to 2022 were sourced from the Web of Science Core Collection. For the assessment of publication attributes-including contributing countries/regions (e.g., US states, provinces, and major cities in China) in comparison to others, institutions, departments, authors, journals, and keywords-we employed bibliometric visualization tools combined with the FLCA algorithm. The analysis findings, inclusive of present research status, prospective trends, and 3 influential articles, were presented through bibliometrics with visualizations.
    Results: We identified 1050 publications from 92 countries/regions. An initial gradual rise in publication numbers was observed until 2019, marking a pivotal juncture. Prominent contributors in research, based on criteria such as regions, institutions, departments, and authors, were Beijing (China), Beijing Univ Chinese Med (China), the Department of Rehabilitation Medicine, and Lidian Chen (Fujian). The journal "Evid.-based Complement Altern" emerged as the most productive. The FLCA algorithm was effectively employed for co-word and author collaboration analyses. Furthermore, we detail the prevailing research status, anticipated trends, and 3 standout articles via bibliometrics.
    Conclusion: Acupuncture for stroke presents a vast research avenue. It is imperative for scholars from various global regions and institutions to transcend academic boundaries to foster dialogue and cooperation. For forthcoming bibliometric investigations, the application of the FLCA algorithm for cluster analysis is advocated.
    MeSH term(s) Humans ; Prospective Studies ; Acupuncture Therapy ; Stroke/therapy ; Stroke Rehabilitation ; Bibliometrics
    Language English
    Publishing date 2023-12-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80184-7
    ISSN 1536-5964 ; 0025-7974
    ISSN (online) 1536-5964
    ISSN 0025-7974
    DOI 10.1097/MD.0000000000035332
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Paper-based colorimetric sensor using Photoshop and a smartphone app for the quantitative detection of carbofuran.

    Wu, Kuo-Hui / Huang, Wen-Chien / Wang, Je-Chuang / Wang, Shih-Hsien

    Analytical methods : advancing methods and applications

    2024  Volume 16, Issue 7, Page(s) 1043–1049

    Abstract: We developed a smartphone-assisted microchemistry analyzer for the quantitative detection of carbofuran using a paper-based colorimetric sensor, Photoshop software, and a smartphone app. The changes in color of the carbofuran enzymatic reaction in the ... ...

    Abstract We developed a smartphone-assisted microchemistry analyzer for the quantitative detection of carbofuran using a paper-based colorimetric sensor, Photoshop software, and a smartphone app. The changes in color of the carbofuran enzymatic reaction in the paper-based sensor were captured and analyzed using a smartphone-controlled analyzer with an LED light source and a smartphone camera. The high accuracy of this method was demonstrated for the determination of carbofuran with a linear response in the range 0.05-1.0 ppm and limits of detection (LOD) of 0.02 and 0.018 ppm using Photoshop and smartphone app colorimetric analysis, respectively. These two methods not only show the high sensitivity and highly quantitative relationships between the concentrations of commercial carbofuran and characteristic color values of the blue channel in smartphone images but were also applied to infusions of tea. Moreover, the smartphone app is able to GPS tag the location of the test and transmit the results to a website that displays quantitative results from carbofuran samples on a map.
    MeSH term(s) Mobile Applications ; Carbofuran ; Colorimetry/methods ; Smartphone ; Microchemistry
    Chemical Substances Carbofuran (SKF77S6Y67)
    Language English
    Publishing date 2024-02-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2515210-5
    ISSN 1759-9679 ; 1759-9660
    ISSN (online) 1759-9679
    ISSN 1759-9660
    DOI 10.1039/d3ay02211k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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