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  1. Article ; Online: Clostridium Perfringens Infection in a Febrile Patient with Severe Hemolytic Anemia.

    Hashiba, Masamitsu / Tomino, Atsutoshi / Takenaka, Nobuyoshi / Hattori, Tomonori / Kano, Hideki / Tsuda, Masanobu / Takeyama, Naoshi

    The American journal of case reports

    2016  Volume 17, Page(s) 219–223

    Abstract: Background: Clostridium perfringens (C. perfringens) can cause various infections, including gas gangrene, crepitant cellulitis, and fasciitis. While C. perfringens sepsis is uncommon, it is often rapidly fatal because the alpha toxin of this bacterium ... ...

    Abstract Background: Clostridium perfringens (C. perfringens) can cause various infections, including gas gangrene, crepitant cellulitis, and fasciitis. While C. perfringens sepsis is uncommon, it is often rapidly fatal because the alpha toxin of this bacterium induces massive intravascular hemolysis by disrupting red blood cell membranes.
    Case report: We present the case of a male patient with diabetes who developed a fatal liver abscess with massive intravascular hemolysis and septic shock caused by toxigenic C. perfringens. The peripheral blood smear showed loss of central pallor, with numerous spherocytes. Multiplex PCR only detected expression of the cpa gene, indicating that the pathogen was C. perfringens type A.
    Conclusions: C. perfringens infection should be considered in a febrile patient who has severe hemolytic anemia with a very low MCV, hemolyzed blood sample, and negative Coombs test. The characteristic peripheral blood smear findings may facilitate rapid diagnosis.
    MeSH term(s) Aged, 80 and over ; Anemia, Hemolytic/microbiology ; Clostridium Infections/complications ; Clostridium Infections/diagnosis ; Clostridium perfringens ; Fatal Outcome ; Fever/microbiology ; Humans ; Liver Abscess/microbiology ; Male ; Shock, Septic/microbiology
    Language English
    Publishing date 2016-04-06
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 2517183-5
    ISSN 1941-5923 ; 1941-5923
    ISSN (online) 1941-5923
    ISSN 1941-5923
    DOI 10.12659/ajcr.895721
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Increased PD-1 Expression and Altered T Cell Repertoire Diversity Predict Mortality in Patients with Septic Shock: A Preliminary Study.

    Tomino, Atsutoshi / Tsuda, Masanobu / Aoki, Ruri / Kajita, Yuka / Hashiba, Masamitsu / Terajima, Tsuguaki / Kano, Hideki / Takeyama, Naoshi

    PloS one

    2017  Volume 12, Issue 1, Page(s) e0169653

    Abstract: Sepsis causes impairment of innate and adaptive immunity by multiple mechanisms, including depletion of immune effector cells and T cell exhaustion. Although lymphocyte dysfunction is associated with increased mortality and potential reactivation of ... ...

    Abstract Sepsis causes impairment of innate and adaptive immunity by multiple mechanisms, including depletion of immune effector cells and T cell exhaustion. Although lymphocyte dysfunction is associated with increased mortality and potential reactivation of latent viral infection in patients with septic shock, the relation between viral reactivation and lymphocyte dysfunction is obscure. The objectives of this study were 1) to determine the relation of lymphocyte dysfunction to viral reactivation and mortality, and 2) to evaluate recovery of lymphocyte function during septic shock, including T cell receptor (TCR) diversity and the expression of programmed death 1 (PD-1). In 18 patients with septic shock and latent cytomegalovirus (CMV) infection, serial blood samples were obtained on days 1, 3, and 7 after the onset of shock, and immune cell subsets and receptor expression were characterized by flow cytometry. TCR diversity of peripheral blood mononuclear cells was analyzed by Multi-N-plex PCR, and CMV DNA was quantified using a real-time PCR kit. A decrease of TCR diversity and monocyte HLA-DR expression were observed in the early stage of septic shock, while CD4+ T cells displayed an increase of PD-1 expression. Significant lymphopenia persisted for at least 7 days following the onset of septic shock. Normalization of TCR diversity and PD-1 expression was observed by day 7, except in patients who died. CMV reactivation was detected in 3 of the 18 patients during the first week of their ICU stay and all 3 patients died. These changes are consistent with the early stage of immune cell exhaustion and indicate the importance of normal lymphocyte function for recovery from septic shock. Ongoing lymphocyte dysfunction is associated with CMV reactivation and dissemination, as well as with unfavorable outcomes.
    MeSH term(s) Aged ; Aged, 80 and over ; Biomarkers ; Case-Control Studies ; Cytomegalovirus ; Cytomegalovirus Infections/genetics ; Cytomegalovirus Infections/immunology ; Cytomegalovirus Infections/virology ; Female ; Gene Expression ; HLA-DR Antigens/genetics ; HLA-DR Antigens/immunology ; Humans ; Immunophenotyping ; Male ; Middle Aged ; Phenotype ; Prognosis ; Programmed Cell Death 1 Receptor/genetics ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/metabolism ; Shock, Septic/genetics ; Shock, Septic/immunology ; Shock, Septic/mortality ; T-Lymphocyte Subsets/immunology ; T-Lymphocyte Subsets/metabolism
    Chemical Substances Biomarkers ; HLA-DR Antigens ; Programmed Cell Death 1 Receptor ; Receptors, Antigen, T-Cell
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0169653
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Increased PD-1 Expression and Altered T Cell Repertoire Diversity Predict Mortality in Patients with Septic Shock

    Atsutoshi Tomino / Masanobu Tsuda / Ruri Aoki / Yuka Kajita / Masamitsu Hashiba / Tsuguaki Terajima / Hideki Kano / Naoshi Takeyama

    PLoS ONE, Vol 12, Iss 1, p e

    A Preliminary Study.

    2017  Volume 0169653

    Abstract: Sepsis causes impairment of innate and adaptive immunity by multiple mechanisms, including depletion of immune effector cells and T cell exhaustion. Although lymphocyte dysfunction is associated with increased mortality and potential reactivation of ... ...

    Abstract Sepsis causes impairment of innate and adaptive immunity by multiple mechanisms, including depletion of immune effector cells and T cell exhaustion. Although lymphocyte dysfunction is associated with increased mortality and potential reactivation of latent viral infection in patients with septic shock, the relation between viral reactivation and lymphocyte dysfunction is obscure. The objectives of this study were 1) to determine the relation of lymphocyte dysfunction to viral reactivation and mortality, and 2) to evaluate recovery of lymphocyte function during septic shock, including T cell receptor (TCR) diversity and the expression of programmed death 1 (PD-1). In 18 patients with septic shock and latent cytomegalovirus (CMV) infection, serial blood samples were obtained on days 1, 3, and 7 after the onset of shock, and immune cell subsets and receptor expression were characterized by flow cytometry. TCR diversity of peripheral blood mononuclear cells was analyzed by Multi-N-plex PCR, and CMV DNA was quantified using a real-time PCR kit. A decrease of TCR diversity and monocyte HLA-DR expression were observed in the early stage of septic shock, while CD4+ T cells displayed an increase of PD-1 expression. Significant lymphopenia persisted for at least 7 days following the onset of septic shock. Normalization of TCR diversity and PD-1 expression was observed by day 7, except in patients who died. CMV reactivation was detected in 3 of the 18 patients during the first week of their ICU stay and all 3 patients died. These changes are consistent with the early stage of immune cell exhaustion and indicate the importance of normal lymphocyte function for recovery from septic shock. Ongoing lymphocyte dysfunction is associated with CMV reactivation and dissemination, as well as with unfavorable outcomes.
    Keywords Medicine ; R ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Neutrophil extracellular traps in patients with sepsis.

    Hashiba, Masamitsu / Huq, Aminul / Tomino, Atsutoshi / Hirakawa, Akihiko / Hattori, Tomonori / Miyabe, Hiromichi / Tsuda, Masanobu / Takeyama, Naoshi

    The Journal of surgical research

    2015  Volume 194, Issue 1, Page(s) 248–254

    Abstract: Background: Release of neutrophil extracellular traps (NETs) has been identified as an important aspect of innate immunity. We examined whether sepsis had any influence on ex vivo generation of NETs by neutrophils.: Materials and methods: We isolated ...

    Abstract Background: Release of neutrophil extracellular traps (NETs) has been identified as an important aspect of innate immunity. We examined whether sepsis had any influence on ex vivo generation of NETs by neutrophils.
    Materials and methods: We isolated neutrophils from consecutive patients with sepsis (n = 17) and without sepsis (n = 18) admitted to the intensive care unit. Neutrophils were activated by incubation with phorbol-12-myristate-13-acetate (PMA) to induce release of NETs, and NET formation was assessed by measuring the extracellular DNA level. Immunolabeling and fluorescence imaging were also performed. Extracellular killing of bacteria by NETs was studied by co-culture of Escherichia coli and neutrophils in the presence of a phagocytosis inhibitor. To assess in vivo NET formation, plasma levels of cell-free DNA and histones were measured.
    Results: After stimulation with PMA, neutrophils isolated from septic patients released 4.08 ± 1.02% of their total DNA, whereas neutrophils from nonseptic patients released 29.06 ± 2.94% (P = <0.0001). Immunofluorescent staining of released DNA, elastase, and myeloperoxidase also revealed similar results. Neutrophils from nonseptic patients showed effective extracellular killing of E coli through NETs, whereas neutrophils from septic patients did not (P < 0.001). Plasma levels of cell-free DNA and histones were higher in septic patients than nonseptic patients (P < 0.001).
    Conclusions: The ex vivo generation of NETs is downregulated in neutrophils isolated from patients with sepsis. However, it is unclear whether in vivo NET formation is also impaired during sepsis, so further investigation is necessary.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blood Bactericidal Activity ; Cytokines/blood ; Extracellular Traps/physiology ; Female ; Histones/blood ; Humans ; Male ; Middle Aged ; Neutrophils/cytology ; Sepsis/immunology
    Chemical Substances Cytokines ; Histones
    Language English
    Publishing date 2015-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80170-7
    ISSN 1095-8673 ; 0022-4804
    ISSN (online) 1095-8673
    ISSN 0022-4804
    DOI 10.1016/j.jss.2014.09.033
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 178: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  5. Article: [A case of epithelioid hemangioendothelioma of the liver: detection by FDG-PET].

    Katsushima, Shinji / Komeda, Toshiki / Endoh, Bunji / Mizumoto, Yoshinori / Watanabe, Toru / Shima, Nobuko / Adachi, Eishi / Kitaoka, Syuji / Hashiba, Masamitsu / Tomono, Teruko / Esaka, Naoki / Ohta, Yoshiyuki / Tanaka, Yoshihito / Morimoto, Hiroyuki / Kuroda, Eriko / Ohta, Rei / Maekawa, Takama / Minamiguchi, Sachiko

    Nihon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology

    2011  Volume 106, Issue 11, Page(s) 1650–1659

    Abstract: A 57 year-old woman was admitted for focal accumulation of 18F-fluorodeoxyglucose (FDG) in the liver detected by positron emission tomography (PET). A 25- mm hypovascular tumor was detected by computed tomography. Tumor biopsy revealed many atypical ... ...

    Abstract A 57 year-old woman was admitted for focal accumulation of 18F-fluorodeoxyglucose (FDG) in the liver detected by positron emission tomography (PET). A 25- mm hypovascular tumor was detected by computed tomography. Tumor biopsy revealed many atypical cells with positive staining for factor VIII-related-antigen in sinusoids. Right lobectomy was performed and the tumor was diagnosed as epithelioid hemangioendothelioma (EHE) pathologically. We demonstrated that FDG-PET was useful for the diagnosis of EHE and making deciding on therapeutic strategy.
    MeSH term(s) Female ; Fluorodeoxyglucose F18 ; Hemangioendothelioma, Epithelioid/diagnostic imaging ; Humans ; Liver Neoplasms/diagnostic imaging ; Middle Aged ; Positron-Emission Tomography
    Chemical Substances Fluorodeoxyglucose F18 (0Z5B2CJX4D)
    Language Japanese
    Publishing date 2011-06-07
    Publishing country Japan
    Document type Case Reports ; Journal Article
    ZDB-ID 708695-7
    ISSN 1349-7693 ; 0446-6586
    ISSN (online) 1349-7693
    ISSN 0446-6586
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ui V Zs.34: Show issues Location:
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    Zs.MO 477: Show issues

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