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  1. Article ; Online: Clostridium difficile in faeces from healthy dogs and dogs with diarrhea.

    Wetterwik, Karl-Johan / Trowald-Wigh, Gunilla / Fernström, Lise-Lotte / Krovacek, Karel

    Acta veterinaria Scandinavica

    2013  Volume 55, Page(s) 23

    Abstract: Background: This study was conducted to evaluate the faecal occurrence and characterization of Clostridium difficile in clinically healthy dogs (N = 50) and in dogs with diarrhea (N = 20) in the Stockholm-Uppsala region of Sweden.: Findings: ... ...

    Abstract Background: This study was conducted to evaluate the faecal occurrence and characterization of Clostridium difficile in clinically healthy dogs (N = 50) and in dogs with diarrhea (N = 20) in the Stockholm-Uppsala region of Sweden.
    Findings: Clostridium difficile was isolated from 2/50 healthy dogs and from 2/20 diarrheic dogs. Isolates from healthy dogs were negative for toxin A and B and for the tcdA and tcdB genes. Both isolates from diarrheic dogs were positive for toxin B and for the tcdA and tcdB genes. The C. difficile isolates from healthy dogs had PCR ribotype 009 (SE-type 6) and 010 (SE-type 3) whereas both isolates from dogs with diarrhoea had the toxigenic ribotype 014 (SE-type 21). One of the isolates from healthy dogs was initially resistant to metronidazole.
    Conclusions: This study revealed presence of toxigenic C. difficile in faecal samples of diarrheic dogs and low number of non- toxigenic isolates in healthy dogs from Uppsala-Stockholm region in Sweden. However, more comprehensive studies are warranted to investigate the role of C. difficile in gastrointestinal disease in dogs.
    MeSH term(s) Animals ; Anti-Bacterial Agents/pharmacology ; Clostridioides difficile/drug effects ; Clostridioides difficile/genetics ; Clostridioides difficile/isolation & purification ; Clostridium Infections/epidemiology ; Clostridium Infections/microbiology ; Clostridium Infections/veterinary ; Diarrhea/epidemiology ; Diarrhea/microbiology ; Diarrhea/veterinary ; Dog Diseases/epidemiology ; Dog Diseases/microbiology ; Dogs ; Drug Resistance, Bacterial ; Feces/microbiology ; Ribotyping ; Sweden/epidemiology
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2013-03-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 40137-7
    ISSN 1751-0147 ; 0044-605X
    ISSN (online) 1751-0147
    ISSN 0044-605X
    DOI 10.1186/1751-0147-55-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Clostridium difficile in faeces from healthy dogs and dogs with diarrhea

    Wetterwik, Karl-Johan / Gunilla Trowald-Wigh / Lise-Lotte Fernström / Karel Krovacek

    Acta veterinaria scandinavica. 2013 Dec., v. 55, no. 1

    2013  

    Abstract: BACKGROUND: This study was conducted to evaluate the faecal occurrence and characterization of Clostridium difficile in clinically healthy dogs (N = 50) and in dogs with diarrhea (N = 20) in the Stockholm-Uppsala region of Sweden. FINDINGS: Clostridium ... ...

    Abstract BACKGROUND: This study was conducted to evaluate the faecal occurrence and characterization of Clostridium difficile in clinically healthy dogs (N = 50) and in dogs with diarrhea (N = 20) in the Stockholm-Uppsala region of Sweden. FINDINGS: Clostridium difficile was isolated from 2/50 healthy dogs and from 2/20 diarrheic dogs. Isolates from healthy dogs were negative for toxin A and B and for the tcdA and tcdB genes. Both isolates from diarrheic dogs were positive for toxin B and for the tcdA and tcdB genes. The C. difficile isolates from healthy dogs had PCR ribotype 009 (SE-type 6) and 010 (SE-type 3) whereas both isolates from dogs with diarrhoea had the toxigenic ribotype 014 (SE-type 21). One of the isolates from healthy dogs was initially resistant to metronidazole. CONCLUSIONS: This study revealed presence of toxigenic C. difficile in faecal samples of diarrheic dogs and low number of non- toxigenic isolates in healthy dogs from Uppsala-Stockholm region in Sweden. However, more comprehensive studies are warranted to investigate the role of C. difficile in gastrointestinal disease in dogs.
    Keywords Clostridium difficile ; diarrhea ; dog diseases ; dogs ; feces ; genes ; metronidazole ; polymerase chain reaction ; toxins ; Sweden
    Language English
    Dates of publication 2013-12
    Size p. 23.
    Publishing place BioMed Central
    Document type Article
    ZDB-ID 40137-7
    ISSN 1751-0147 ; 0044-605X
    ISSN (online) 1751-0147
    ISSN 0044-605X
    DOI 10.1186/1751-0147-55-23
    Database NAL-Catalogue (AGRICOLA)

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