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Article ; Online: Initial SARS-CoV-2 RNA load in nasopharyngeal specimens is not associated with severity and midterm resolution of pneumonia in COVID-19 patients.

Costa, Rosa / Signes-Costa, Jaime / Olea, Beatriz / Tarraso, Julia / Carbonell, Nieves / Blasco, María Luisa / Navarro, David

The Journal of infection

2022 Volume 85, Issue 6, Page(s) 702–769

MeSH term(s)	Humans ; COVID-19 ; SARS-CoV-2 ; RNA, Viral/genetics ; Nasopharynx ; COVID-19 Testing ; Viral Load
Chemical Substances	RNA, Viral
Language	English
Publishing date	2022-10-28
Publishing country	England
Document type	Letter
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2022.10.031
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Zs.A 1501: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 48: Show issues

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Article ; Online: The Biofire® Filmarray® Pneumonia Plus panel for management of lower respiratory tract infection in mechanically-ventilated patients in the COVID-19 era: a diagnostic and cost-benefit evaluation.

Ferrer, Josep / Clari, María Ángeles / Giménez, Estela / Carbonell, Nieves / Torres, Ignacio / Blasco, María Luisa / Albert, Eliseo / Navarro, David

Diagnostic microbiology and infectious disease

2022 Volume 105, Issue 2, Page(s) 115847

Abstract: We assessed the diagnostic performance of the Biofire® Filmarray® Pneumonia Plus panel (FA-PP) compared to standard culture in Intensive Care Unit patients with suspected ventilator-associated lower respiratory tract infection in the COVID-19 era. We ... ...

Abstract	We assessed the diagnostic performance of the Biofire® Filmarray® Pneumonia Plus panel (FA-PP) compared to standard culture in Intensive Care Unit patients with suspected ventilator-associated lower respiratory tract infection in the COVID-19 era. We determined whether its implementation in routine diagnostic algorithms would be cost-beneficial from a hospital perspective. Of 163 specimens, 96 (59%) returned negative results with FA-PP and conventional culture, and 29 specimens (17.8%) were positive with both diagnostic methods and yielded concordant qualitative bacterial identification/isolation. Thirty-nine specimens (23.9%) gave discordant results (positive via FA-PP and negative via culture). Real-life adjustments of empirical antimicrobial therapy (EAT) after FA-PP results resulted in additional costs beyond EAT alone of 1868.7 €. Adequate EAT adjustments upon FA-PP results would have resulted in a saving of 6675.8 €. In conclusion, the data presented supports the potential utility of FA-PP for early EAT adjustment in patients with ventilator-associated lower respiratory tract infection.
MeSH term(s)	Humans ; Bacteria ; COVID-19 ; Respiration, Artificial ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/drug therapy ; Respiratory Tract Infections/microbiology ; Anti-Infective Agents ; COVID-19 Testing
Chemical Substances	Anti-Infective Agents
Language	English
Publishing date	2022-11-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2022.115847
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Zs.A 1845: Show issues

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Article ; Online: A Shocking Experience.

Mariño, Robert Blasco / Martinez, Iñigo Soteras / Paños Gozalo, Maria Luisa / Zafren, Ken

Wilderness & environmental medicine

2021 Volume 32, Issue 3, Page(s) 383–384

MeSH term(s)	Electric Countershock
Language	English
Publishing date	2021-06-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	1238909-2
ISSN	1545-1534 ; 1080-6032
ISSN (online)	1545-1534
ISSN	1080-6032
DOI	10.1016/j.wem.2021.04.003
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Zs.A 3025: Show issues

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Article ; Online: Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions.

Pérez-Piñeiro, Javier / Sánchez-Cea, Fernando / Arce, Mariana P / Lado-Touriño, Isabel / Rojas-Cervantes, María Luisa / Gilsanz, María Fuencisla / Gallach-Pérez, Darío / Blasco, Rodrigo / Barrios-Bermúdez, Niurka / Cerpa-Naranjo, Arisbel

Journal of xenobiotics

2023 Volume 13, Issue 1, Page(s) 90–101

Abstract: In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is ... ...

Abstract	In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic force microscopy, and ultraviolet visible spectroscopy, comparing the starting nanomaterials with the nanomaterials in contact with the biological material, i.e., bovine fetal serum. The different experiments were performed at different concentrations of nanomaterial (0.125-0.5 mg/mL) and BSA (0.01-0.04 mg/mL), at different incubation times (5-360 min), with and without PEG, and at different temperatures (25-40 °C). The SEM results show that BSA is adsorbed on the surface of the graphene oxide nanomaterial. Using UV-Vis spectrophotometry, the characteristic absorption peaks of BSA are observed at 210 and 280 nm, corroborating that the protein has been adsorbed. When the time increases, the BSA protein can be detached from the nanomaterial due to a desorption process. The stability of the dispersions is reached at a pH between 7 and 9. The dispersions behave like a Newtonian fluid with viscosity values between 1.1 and 1.5 mPa·s at a temperature range of 25 to 40 °C. The viscosity values decrease as the temperature increases.
Language	English
Publishing date	2023-02-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649720-7
ISSN	2039-4713 ; 2039-4713
ISSN (online)	2039-4713
ISSN	2039-4713
DOI	10.3390/jox13010008
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Article: "CAPA in Progress": A New Real-Life Approach for the Management of Critically Ill COVID-19 Patients.

Carbonell, Nieves / Alcaráz, María Jesús / Serrano-Lázaro, Ainhoa / Rodríguez-Gimillo, María / Sánchez Ramos, David / Ros, Francisco / Ferrer, Josep / Blasco, María Luisa / Navarro, David / Clari, María Ángeles

Biomedicines

2022 Volume 10, Issue 7

Abstract: 1) Background: COVID-19-associated pulmonary aspergillosis (CAPA) has worsened the prognosis of patients with pneumonia and acute respiratory distress syndrome admitted to the intensive care unit (ICU). The lack of specific diagnosis criteria is an ... ...

Abstract	(1) Background: COVID-19-associated pulmonary aspergillosis (CAPA) has worsened the prognosis of patients with pneumonia and acute respiratory distress syndrome admitted to the intensive care unit (ICU). The lack of specific diagnosis criteria is an obstacle to the timely initiation of appropriate antifungal therapy. Tracheal aspirate (TA) has been employed under special pandemic conditions. Galactomannan (GM) antigens are released during active fungal growth. (2) Methods: We proposed the term "CAPA in progress" (CAPA-IP) for diagnosis at an earlier stage by GM testing on TA in a specific population admitted to ICU presenting with clinical deterioration. A GM threshold ≥0.5 was set as the mycological inclusion criterion. This was followed by a pre-emptive short-course antifungal. (3) Results: We prospectively enrolled 200 ICU patients with COVID-19. Of these, 164 patients (82%) initially required invasive mechanical ventilation and GM was tested in TA in 93 patients. A subset of 19 patients (11.5%) fulfilled the CAPA-IP criteria at a median of 9 days after ICU admittance. The median GM value was 3.25 ± 2.82. CAPA-IP cases showed significantly higher ICU mortality [52.6% (10/19) vs. 34.5% (50/145),
Language	English
Publishing date	2022-07-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10071683
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Article ; Online: Monitoring of Torque Teno virus DNAemia in critically ill COVID-19 patients: May it help to predict clinical outcomes?

Forqué, Lorena / Albert, Eliseo / Giménez, Estela / Torres, Ignacio / Carbonell, Nieves / Ferreres, José / Blasco, María Luisa / Navarro, David

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

2022 Volume 148, Page(s) 105082

Abstract: Background: Torque teno virus (TTV) DNA load in plasma directly associates with the net state of immunosuppression and inflammation in different clinical settings, including transplantation and chronic inflammatory diseases.: Objectives: We ... ...

Abstract	Background: Torque teno virus (TTV) DNA load in plasma directly associates with the net state of immunosuppression and inflammation in different clinical settings, including transplantation and chronic inflammatory diseases. Objectives: We investigated whether plasma TTV DNA load may predict the occurrence of certain infectious events and overall mortality in critically ill COVID-19 patients. Patients and methods: 50 patients (median age, 65.5 years) were recruited. TTV DNA load was quantitated in serial plasma specimens by real-time PCR. Serum levels of interleukin-6, C-reactive protein, ferritin, lactate dehydrogenase, Gamma-Glutamyl Transferase (GGT), alanine transaminase (ALT) and aspartate transaminase (AST) and absolute lymphocyte counts (ALC) in paired specimens were available. Nosocomial bloodstream infections and ventilator-associated pneumonia and overall mortality were the clinical outcomes. Results: TTV DNA was detected in 38 patients (76%). A weak inverse correlation (Rho=-0.28; P = 0.004) was observed between TTV DNA loads and ALC. No direct correlation was found between TTV DNA load and serum levels of any of the above biomarkers. Patients with detectable TTV DNA had an increased risk of subsequently developing infectious events (HR 9.28; 95% CI, 1.29-69.5; P = 0.03). A trend (P = 0.05) towards higher TTV DNA area under a curve between days 7 and 17 after ICU admission (AUC Conclusion: Our findings suggested that plasma TTV DNA load monitoring may be helpful for predicting the occurrence of severe nosocomial infections and mortality in critically ill COVID-19 patients.
MeSH term(s)	Aged ; COVID-19 ; Critical Illness ; DNA Virus Infections ; DNA, Viral ; Humans ; SARS-CoV-2 ; Torque teno virus/genetics ; Viral Load
Chemical Substances	DNA, Viral
Language	English
Publishing date	2022-01-23
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2022.105082
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Zs.A 3790: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
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Article: Estudio de las habilidades sociales o de la conducta asertiva en el alumnado de la Escuela Universitaria de Enfermería de Huesca.

Mata Blasco, María Luisa

Revista de enfermeria (Barcelona, Spain)

2018 Volume 39, Issue 9, Page(s) 36–43

Abstract: Objective: To know the level and characteristics of social skills (SS) in nursing students from the Escuela Universitaria de Enfermería of Huesca (EUEH) during undergraduate training and its relationship with personal, educational and employment ... ...

Title translation	Study of Social Skills or Assertive Behaviour in the Students of the Escuela Universitaria de Enfermería of Huesca (Spain).
Abstract	Objective: To know the level and characteristics of social skills (SS) in nursing students from the Escuela Universitaria de Enfermería of Huesca (EUEH) during undergraduate training and its relationship with personal, educational and employment variables. Method: A descriptive cross-sectional study was conducted on 126 students enrolled in the course 2012-2013. As an evaluation instrument the Social Skills Scale of Gismero [1] was used. Results: The overall level of SS is 96.15 (SD = 12.06), which is in the 75th percentile of the sample scales of the Social Skills Scale. The Students of the second course are significantly more skilled (< 0.005) in dimension "defend their rights" (DII), with a score of 15.17 (SD = 1.95). In the students under 21 years, are significant differences <0.033) for the dimension "express requests' (DV) with result of 14.82 (SD = 2.73). Students without prior experience, with health scores of 14.84 (SD = 2.76), show significant differences (<0.002) in expression of requests (DV). Conclusions: The overall level of SS of our students is superior to the sample scales of the Social Skills Scale. In the assessment of Social Skills, deficits do not appear in any of the evaluated dimensions. The SS taken together have not been modified by the variables analyzed; only have responded to the effect of variables "grade", "age' and "previous experience", DII ("ability to defend their rights") and DV ("ability to express requests").
MeSH term(s)	Assertiveness ; Cross-Sectional Studies ; Female ; Humans ; Male ; Schools, Nursing ; Self Report ; Social Skills ; Spain ; Students, Nursing/psychology ; Young Adult
Language	Spanish
Publishing date	2018-09-04
Publishing country	Spain
Document type	Journal Article ; Observational Study
ZDB-ID	1201957-4
ISSN	0210-5020
ISSN	0210-5020
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Zs.A 4067: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: “CAPA in Progress”

Nieves Carbonell / María Jesús Alcaráz / Ainhoa Serrano-Lázaro / María Rodríguez-Gimillo / David Sánchez Ramos / Francisco Ros / Josep Ferrer / María Luisa Blasco / David Navarro / María Ángeles Clari

Biomedicines, Vol 10, Iss 7, p

A New Real-Life Approach for the Management of Critically Ill COVID-19 Patients

2022 Volume 1683

Abstract	(1) Background: COVID-19-associated pulmonary aspergillosis (CAPA) has worsened the prognosis of patients with pneumonia and acute respiratory distress syndrome admitted to the intensive care unit (ICU). The lack of specific diagnosis criteria is an obstacle to the timely initiation of appropriate antifungal therapy. Tracheal aspirate (TA) has been employed under special pandemic conditions. Galactomannan (GM) antigens are released during active fungal growth. (2) Methods: We proposed the term “CAPA in progress” (CAPA-IP) for diagnosis at an earlier stage by GM testing on TA in a specific population admitted to ICU presenting with clinical deterioration. A GM threshold ≥0.5 was set as the mycological inclusion criterion. This was followed by a pre-emptive short-course antifungal. (3) Results: We prospectively enrolled 200 ICU patients with COVID-19. Of these, 164 patients (82%) initially required invasive mechanical ventilation and GM was tested in TA in 93 patients. A subset of 19 patients (11.5%) fulfilled the CAPA-IP criteria at a median of 9 days after ICU admittance. The median GM value was 3.25 ± 2.82. CAPA-IP cases showed significantly higher ICU mortality [52.6% (10/19) vs. 34.5% (50/145), p = 0.036], as well as a much longer median ICU stay than those with a normal GM index [27 (7–64) vs. 11 (9–81) days, p = 0.008]. All cases were treated with a pre-emptive systemic antifungal for a median time of 19 (3–39) days. (4) Conclusions: CAPA-IP highlights a new real-life early approach in the field of fungal stewardship in ICU programs.
Keywords	COVID-19 ; critically ill patients ; CAPA ; “CAPA in progress” ; galactomannan ; Aspergillus spp ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: SARS-CoV-2-reactive IFN-γ-producing CD4

Olea, Beatriz / Albert, Eliseo / Giménez, Estela / Torres, Ignacio / Amat, Paula / Remigia, María José / Alberola, Juan / Carbonell, Nieves / Ferreres, José / Blasco, María Luisa / Navarro, David

Scientific reports

2022 Volume 12, Issue 1, Page(s) 14271

Abstract: We examined the relationship between peripheral blood levels of SARS-CoV-2 S (Spike protein)1/M (Membrane protein)-reactive IFN-γ-producing ... ...

Abstract	We examined the relationship between peripheral blood levels of SARS-CoV-2 S (Spike protein)1/M (Membrane protein)-reactive IFN-γ-producing CD4
MeSH term(s)	Aged ; Antibodies, Viral ; CD4-Positive T-Lymphocytes ; CD8-Positive T-Lymphocytes ; COVID-19 ; Critical Illness ; Female ; Humans ; Immunoglobulin G ; Male ; RNA, Viral ; SARS-CoV-2
Chemical Substances	Antibodies, Viral ; Immunoglobulin G ; RNA, Viral
Language	English
Publishing date	2022-08-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-18659-x
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Article ; Online: Enhanced Photodegradation of Synthetic Dyes Mediated by Ag 3 PO 4 -Based Semiconductors under Visible Light Irradiation

Alice Pavanello / Alejandro Blasco / Peter F. Johnston / Miguel A. Miranda / Maria Luisa Marin

Catalysts, Vol 10, Iss 774, p

2020 Volume 774

Abstract: Four silver phosphate-based materials were successfully synthesized, characterized, and evaluated, together with TiO 2 , in the photodegradation of synthetic dyes (tartrazine, Orange II, rhodamine, and Brilliant Blue FCF) under two irradiation sources ... ...

Abstract	Four silver phosphate-based materials were successfully synthesized, characterized, and evaluated, together with TiO 2 , in the photodegradation of synthetic dyes (tartrazine, Orange II, rhodamine, and Brilliant Blue FCF) under two irradiation sources centered at 420 and 450 nm. Scanning Electron Microscopy (SEM) images showed different topologies of the synthesized materials, whereas diffuse reflectance spectra demonstrated that they display absorption up to 500 nm. Degradation experiments were performed in parallel with the silver materials and TiO 2 . Upon irradiation centered at 420 nm, the abatement of the dyes was slightly more efficient in the case of TiO 2 —except for Orange II. Nevertheless, upon irradiation centered at 450 nm, TiO 2 demonstrated complete inefficiency and silver phosphates accomplished the complete abatement of the dyes—except for Brilliant Blue FCF. A careful analysis of the achieved degradation of dyes revealed that the main reaction mechanism involves electron transfer to the photogenerated holes in the valence band of silver photocatalysts, together with the direct excitation of dyes and the subsequent formation of reactive species. The performance of TiO 2 was only comparable at the shorter wavelength when hydroxyl radicals could be formed; however, it could not compete under irradiation at 450 nm since the formed superoxide anion is not as reactive as hydroxyl radicals.
Keywords	conduction band ; hole ; hydroxyl radical ; mechanism ; superoxide anion ; titanium dioxide ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
Subject code	290
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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