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  1. Article ; Online: Ling-Gui-Zhu-Gan decoction protects against doxorubicin-induced myocardial injury by downregulating ferroptosis.

    Yang, Ya-Li / Zhao, Chuan-Zhi / Zhao, Chun-Chun / Wen, Zhong-Yu / Ma, Yao-Yao / Zhao, Xiao-Ni / Wang, Liang / Huang, Jin-Ling / Zhou, Peng

    The Journal of pharmacy and pharmacology

    2024  Volume 76, Issue 4, Page(s) 405–415

    Abstract: Objective: To investigate the mechanism of Ling-Gui-Zhu-Gan decoction (LGZGD) protects against ...

    Abstract Objective: To investigate the mechanism of Ling-Gui-Zhu-Gan decoction (LGZGD) protects against doxorubicin (DOX)-induced myocardial injury.
    Methods: In vivo experiment, rats were divided into six groups: normal group, model group (15 mg/kg, DOX), Dex group(150 mg/kg, Dex), LGZGD-L group (2.1 g/kg), LGZGD-M group (4.2 g/kg), and LGZGD-H group (8.4 g/kg). We used HE and Masson staining to observe the histopathological changes, echocardiography to assess the cardiac function, and western blot and RT-qPCR to detect the expressions of Nrf2, GPX4, Fpn1, and Ptgs2. In vitro experiment, we used immunofluorescence to detect ROS production, and RT-qPCR to detect gene expression of GPX4, Fpn1, and Ptgs2.
    Key findings: In vivo, LGZGD improved cardiac systolic function. LGZGD significantly reduced MDA, LDH, and CK levels, increased SOD activity, enhanced the protein expression of Nrf2, GPX4, and Fpn1, and decreased Ptgs2 levels. In vitro, LGZGD-containing serum significantly reduced ROS, increased the gene expression of GPX4 and Fpn1, and decreased the gene expression of Ptgs2. Furthermore, compared with the LGZGD (si-NC) group, the LGZGD (si-Nrf2) group had decreased gene expression of Nrf2, GPX4, and Fpn1 and increased gene expression of Ptgs2.
    Conclusions: LGZGD can ameliorate DOX-cardiotoxicity by activating the Nrf2 signaling pathway and inhibiting ferroptosis in cardiomyocytes.
    MeSH term(s) Rats ; Animals ; Ferroptosis ; Cyclooxygenase 2 ; NF-E2-Related Factor 2 ; Reactive Oxygen Species ; Doxorubicin/toxicity ; Plant Extracts
    Chemical Substances ling-gui-zhu-gan decoction ; Cyclooxygenase 2 (EC 1.14.99.1) ; NF-E2-Related Factor 2 ; Reactive Oxygen Species ; Doxorubicin (80168379AG) ; Plant Extracts
    Language English
    Publishing date 2024-01-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 3107-0
    ISSN 2042-7158 ; 0022-3573 ; 0373-1022
    ISSN (online) 2042-7158
    ISSN 0022-3573 ; 0373-1022
    DOI 10.1093/jpp/rgae005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Ling Zhi-8, a fungal immunomodulatory protein in

    Li, Ju-Pi / Chu, Ching-Liang / Chao, Wan-Ru / Yeh, Cheng-Siang / Lee, Yi-Ju / Chen, Dz-Chi / Yang, Shun-Fa / Chao, Yu-Hua

    Aging

    2023  Volume 15, Issue 9, Page(s) 3621–3634

    Abstract: ... for its clinical utility. Ling Zhi-8 (LZ-8), a fungal immunomodulatory protein in ...

    Abstract CPT-11 (Irinotecan) remains an important chemotherapeutic agent against various solid tumors nowadays. Potential adverse effects, especially gastrointestinal toxicities, are the main limiting factor for its clinical utility. Ling Zhi-8 (LZ-8), a fungal immunomodulatory protein in
    MeSH term(s) Mice ; Animals ; Reishi ; Irinotecan ; Claudin-1/genetics
    Chemical Substances Irinotecan (7673326042) ; Claudin-1
    Language English
    Publishing date 2023-05-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.204695
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Rapid classification and identification of chemical compositions of Pu-zhi-hui-ling decoction by UHPLC-Q-Orbitrap HRMS.

    Shao, Jia / Zheng, Yanxue / Wang, Yuanyuan / Li, Guohui / Wei, Jinxia / Cheng, Wenbo / Li, Yubo

    Natural product research

    2024  , Page(s) 1–10

    Abstract: Pu-zhi-hui-ling decoction (PZHLD) is a traditional Chinese medicine (TCM) formula for the treatment ...

    Abstract Pu-zhi-hui-ling decoction (PZHLD) is a traditional Chinese medicine (TCM) formula for the treatment of Alzheimer's disease (AD), but its chemical composition has not been reported. In this study, we aimed to establish a mass spectrometry (MS) analysis method for rapid classification and identification of the chemical constituents in PZHLD. The sample was analysed by ultrahigh-performance liquid chromatography coupled to quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). The chemical constituents of PZHLD were identified based on accurate MS data, fragmentation characteristics of MS/MS, and reference information described in the literature. A total of 123 chemical constituents were identified. In addition, we summarised the fragmentation pathways of the chemical constituents in PZHLD. Our finding might lay the foundation for the further pharmacodynamic study and clinical application of PZHLD.
    Language English
    Publishing date 2024-01-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2023.2299302
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Inhibitory Potential of Shen-Shuai-Ling Formulation on Renal Interstitial Fibrosis via Upregulation of PLZF.

    Song, Na / Tu, Haitao / Li, Ying / Xiong, Weijian / Zhang, Ling / Liu, Hong / Ding, Weisen / Long, Mei / Ren, Dewei / Zhong, Jin

    Evidence-based complementary and alternative medicine : eCAM

    2022  Volume 2022, Page(s) 5967804

    Abstract: ... which seriously affects people's health. As a traditional Chinese medicine, Shen-Shuai-Ling Formulation (SSLF) has ...

    Abstract Background: Renal interstitial fibrosis (RIF) is an important cause of kidney disease, which seriously affects people's health. As a traditional Chinese medicine, Shen-Shuai-Ling Formulation (SSLF) has obvious kidney function. However, the therapeutic effect of SSLF on RIF and its molecular mechanism are still unclear.
    Methods: First, the potential targets and pathways of SSLF for RIF were predicted by network pharmacology, and then, the binding of luteolin and target protein to SSLF was verified by molecular docking and Co-IP experiments. Finally, the effects of SSLF and luteolin on PLZF and (Pro) renin receptor (PRR) were verified by western blot and qPCR experiments. Angiotensin (Ang)-1, Ang-2, and transforming growth factor-
    Results: Through the drug-active component-target network diagram, we found that luteolin has the most connections, and promyelocytic leukemia zinc finger (PLZF) is the target protein. GO analysis and KEGG pathway analysis of targets were performed using Cytoscape ClueGO. Molecular docking experiments and Co-IP are used to prove that luteolin and PLZF can be combined. Western blot and qPCR results showed that both SSLF and luteolin significantly upregulated the expression of PLZF and decreased the levels of PRR, Ang-1, Ang-2, and TGF-
    Conclusions: SSLF inhibits PRR and renal interstitial fibers by the upregulation of PLZF levels.
    Language English
    Publishing date 2022-03-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2022/5967804
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Re: Ling L and Li X. A dorsalis pedis venous flap containing a U-shaped venous arch for the reconstruction of fingertip defects. J Hand Surg Eur. 2022. DOI: 10.1177/17531934221098064.

    Ling, Li

    The Journal of hand surgery, European volume

    2022  Volume 48, Issue 1, Page(s) 75–76

    MeSH term(s) Humans ; Surgical Flaps ; Plastic Surgery Procedures ; Foot ; Fingers
    Language English
    Publishing date 2022-11-17
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 2272801-6
    ISSN 2043-6289 ; 1753-1934
    ISSN (online) 2043-6289
    ISSN 1753-1934
    DOI 10.1177/17531934221137782
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Inhibition of estrogen sulfation by Xian-Ling-Gu-Bao capsule.

    He, Liangliang / Chen, Chanjuan / Duan, Shuyi / Li, Yang / Li, Chuan / Yao, Xinsheng / Gonzalez, Frank J / Qin, Zifei / Yao, Zhihong

    The Journal of steroid biochemistry and molecular biology

    2022  Volume 225, Page(s) 106182

    Abstract: Xian-Ling-Gu-Bao capsule (XLGB) is a widely prescribed traditional Chinese medicine used ...

    Abstract Xian-Ling-Gu-Bao capsule (XLGB) is a widely prescribed traditional Chinese medicine used for the treatment of osteoporosis. However, it significantly elevates levels of serum estrogens. Here we aimed to assess the dominant contributors of sulfotransferase (SULT) enzymes to the sulfation of estrogens and identify the effective inhibitors of this pathway in XLGB. First, estrone, 17β-estradiol, and estriol underwent sulfation in human liver S9 extracts. Phenotyping reactions and enzyme kinetics assays revealed that SULT1A1, 1A2, 1A3, 1C4, 1E1, and 2A1 all participated in estrogen sulfation, with SULT1E1 and 1A1 as the most important contributors. The incubation system for these two active enzymes were optimized with Tris-HCl buffer, DL-Dithiothreitol (DTT), MgCl
    MeSH term(s) Humans ; Molecular Docking Simulation ; Sulfotransferases/metabolism ; Polyphenols ; Estrogens
    Chemical Substances Sulfotransferases (EC 2.8.2.-) ; Polyphenols ; Estrogens
    Language English
    Publishing date 2022-09-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1049188-0
    ISSN 1879-1220 ; 0960-0760
    ISSN (online) 1879-1220
    ISSN 0960-0760
    DOI 10.1016/j.jsbmb.2022.106182
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  7. Article: Comparison of pharmacokinetic profiles of seven major bioactive components in normal and non-alcoholic fatty liver disease (NAFLD) rats after oral administration of Ling-Gui-Zhu-Gan decoction by UPLC-MS/MS.

    Nie, Wenlong / Yang, Yang / Li, Ling / Ding, Yue / Chen, Xingmi / Li, Ming / He, Ning / Ji, Guang / Zhang, Yong / Kang, Ping / Zhang, Tong

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1174742

    Abstract: ... in the plasma of rats after the oral administration of Ling-Gui-Zhu-Gan decoction (LGZGD). Besides, this very ...

    Abstract A sensitive and rapid ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was hereby developed for the determination of seven components, namely, glycyrrhizic acid, glycyrrhetinic acid, dehydrotumulosic acid, isoliquiritin, liquiritin, atractylenolide III, and cinnamic acid, in the plasma of rats after the oral administration of Ling-Gui-Zhu-Gan decoction (LGZGD). Besides, this very method was methodologically validated for specificity, linearity, inter-day and intra-day precision, accuracy, matrix effect, extraction recovery, and stability. It was also successfully used for the first time to compare the pharmacokinetic characteristics of the seven components after oral administration of LGZGD to normal rats and non-alcoholic fatty liver disease (NAFLD) rats. The results indicated significant differences between the pharmacokinetic characteristics of normal and NAFLD rats. To further reveal the different pharmacokinetic behaviors, the expressions of enzymes and transporters in the liver of normal and NAFLD rats were detected using UPLC-MS/MS. In the NAFLD rats, UDP-glucuronosyltransferase 1-1 (UGT1A1) and nine transporters were significantly inhibited and a positive correlation was observed between them and the AUC of the major components. The present results indicate that the pharmacokinetic differences between the normal and NAFLD rats might be attributed to the significant lower expression levels of both the metabolic enzyme UGT1A1 and nine transporter proteins in the NAFLD rats than in the normal rats. Meanwhile, UGT1A1 and the nine transporter proteins might be used as potential biomarkers to assess the ameliorative effect of LGZGD on NAFLD, which could provide useful information to guide the clinical application of LGZGD.
    Language English
    Publishing date 2023-05-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1174742
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Network pharmacology and experimental validation to investigate the mechanism of Nao-Ling-Su capsule in the treatment of ischemia/reperfusion-induced acute kidney injury.

    Lin, Yongqiang / Xu, Lili / Lin, Huibin / Cui, Weiliang / Jiao, Yang / Wang, Bing / Li, Huifen / Wang, Xiaojie / Wu, Jichao

    Journal of ethnopharmacology

    2024  Volume 326, Page(s) 117958

    Abstract: Ethnopharmacological relevance: Nao-Ling-Su Capsule (NLSC) is a traditional prescription, which is ...

    Abstract Ethnopharmacological relevance: Nao-Ling-Su Capsule (NLSC) is a traditional prescription, which is composed of fifteen herbs such as epimedium, Polygala tenuifolia, and Schisandra chinensis. It has the effect of strengthening the brain, calming nerves, and protecting the kidney, which has been used clinically for many years to strengthen the brain and kidney. However, the effect of NLSC in the treatment of acute kidney injury (AKI) is still unclear.
    Aim of the study: The present study aims to elucidate the pharmacological actions of NLSC in the treatment of AKI.
    Materials and methods: Molecular targets for NLSC and AKI were obtained from various databases, and then we built networks of interactions between proteins (PPI) by employing string databases. Additionally, we employed the DAVID database to conduct gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Molecular docking was conducted to analyze the interaction between core components and their corresponding core targets. Next, the C57BL male mice model of ischemia/reperfusion damage (IRI) was developed, and the nephridial protective effect of NLSC was evaluated. The accuracy of the expected targets was confirmed using real-time quantitative polymerase chain reaction (RT-qPCR). The renal protective effect of NLSC was assessed using an immortalized human kidney tubular (HK-2) cell culture produced by oxygen-glucose deprivation (OGD).
    Results: Network pharmacology analysis identified 199 common targets from NLSC and AKI. STAT3, HSP90AA1, TP53, MAPK3, JUN, JAK2, and VEGFA could serve as potential drug targets and were associated with JAK2/STAT3 signaling pathway, PI3K-Akt signaling pathway, etc. The molecular docking analysis confirmed significant docking activity between the main bioactive components and core targets, including STAT3 and KIM-1. Moreover, the AKI mice model was successfully established and NLSC pretreatment could improve renal function and alleviate renal damage. NLSC could alleviate renal inflammation and tubular cell apoptosis, and decrease the expression of STAT3 and KIM-1 in AKI mice. In vitro, both NLSC and drug-containing serum may protect HK-2 cells by inhibiting STAT3 signaling, especially STAT3-mediated apoptosis and KIM-1 expression.
    Conclusion: NLSC could alleviate renal inflammation and apoptosis, exerting its beneficial effects by targeting the STAT3/KIM-1 pathway. NLSC is a promising candidate for AKI treatment and provides a new idea and method for the treatment of AKI.
    MeSH term(s) Humans ; Male ; Animals ; Mice ; Mice, Inbred C57BL ; Molecular Docking Simulation ; Network Pharmacology ; Phosphatidylinositol 3-Kinases ; Kidney ; Acute Kidney Injury/drug therapy ; Reperfusion Injury/drug therapy ; Ischemia ; Reperfusion ; Nephritis ; Inflammation ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use
    Chemical Substances Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Drugs, Chinese Herbal
    Language English
    Publishing date 2024-02-22
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117958
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  9. Article ; Online: Qualitative and quantitative studies on chemical constituents of Ling-gui-zhu-gan decoction: In vitro and in vivo.

    Li, Siyue / Gao, Yutong / Wang, Xue / Wang, Zhe / Li, Nan / Shang, Yonglin / Han, Fei / Yu, Jia

    Journal of separation science

    2023  Volume 46, Issue 19, Page(s) e2300465

    Abstract: Ling-gui-zhu-gan decoction has significant therapeutic effects in the treatment of diseases related ... constituents of Ling-gui-zhu-gan decoction in vitro and in vivo by HPLC coupled to Fourier transform ion ... components of Ling-gui-zhu-gan decoction. ...

    Abstract Ling-gui-zhu-gan decoction has significant therapeutic effects in the treatment of diseases related to phlegm and fluid retention. In this study, we aimed to qualitatively characterize the chemical constituents of Ling-gui-zhu-gan decoction in vitro and in vivo by HPLC coupled to Fourier transform ion cyclotron resonance MS, and quantitively determine the contents of typical chemical constituents by HPLC method. As a result, a total of 75 chemical constituents were discovered including 37 flavonoids and their glycosides, 20 saponins, 9 sterols, 3 organic acids and their derivatives, 3 lactones, 2 coumarins, and 1 alcohol. Among them, 17 chemical constituents were specifically identified. Subsequently, an HPLC method was established for simultaneous determination of seven chemical constituents. Finally, a total of 40 prototype components were initially detected by HPLC-MS method in the biological samples of rats after their water extract was orally administrated. Among them, 29, 27, 12, and 32 prototype components were detected in plasma, bile, urine, and feces, respectively. Moreover, 34 metabolites, including 16 phase II metabolites, were detected for the first time. In conclusion, this study lays the foundation for the identification of chemical components in vitro and in vivo and the elucidation of the potential pharmacodynamic components of Ling-gui-zhu-gan decoction.
    Language English
    Publishing date 2023-08-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2047990-6
    ISSN 1615-9314 ; 1615-9306
    ISSN (online) 1615-9314
    ISSN 1615-9306
    DOI 10.1002/jssc.202300465
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: An integrated strategy by absorbed component characterization, pharmacokinetics, and activity evaluation for identification of potential nephroprotective substances in Zhu-Ling decoction.

    Shu, Zhi-Heng / Fan, Cai-Lian / Wei, Hong-Yan / Li, Zi-Ting / Norimoto, Hisayoshi / Tang, Xi-Yang / Yao, Zhi-Hong / Yao, Xin-Sheng / Dai, Yi

    Journal of separation science

    2023  Volume 46, Issue 17, Page(s) e2300331

    Abstract: An efficient strategy for the identification of potential nephroprotective substances in Zhu-Ling ... constituents absorbed components in vivo of Zhu-Ling decoction by using ultra-performance liquid chromatography ... activities in hydrogen peroxide-induced Vero cells. As a result, 111 compounds in Zhu-Ling decoction and 36 ...

    Abstract An efficient strategy for the identification of potential nephroprotective substances in Zhu-Ling decoction has been established with the integration of absorbed components characterization, pharmacokinetics, and activity evaluation. A qualitative method was developed to characterize the chemical constituents absorbed components in vivo of Zhu-Ling decoction by using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. A quantitative method was established and validated for the simultaneous determination of eight compounds in rat plasma by using ultra-performance liquid chromatography-triple quadruple tandem mass spectrometry. Finally, the nephroprotective activities of absorbed components with high exposure were assessed by cell survival rate, superoxide dismutase, and malondialdehyde activities in hydrogen peroxide-induced Vero cells. As a result, 111 compounds in Zhu-Ling decoction and 36 absorbed components were identified in rat plasma and urine, and poricoic acid A, poricoic acid B, alisol A, 16-oxo-alisol A, and dehydro-tumulosic acid had high exposure levels in rat plasma. Finally, poricoic acid B, poricoic acid A, 16-oxo-alisol A, and dehydro-tumulosic acid showed remarkable nephroprotective activity against Vero cells damage induced by hydrogen peroxide. Besides, superoxide dismutase and malondialdehyde activities were obviously regulated in hydrogen peroxide-induced Vero cells by treatment with the four compounds mentioned above. Therefore, these four compounds were considered to be effective substances of Zhu-Ling decoction due to their relatively high exposure in vivo and biological activity. This study provided a chemical basis for the action mechanism of Zhu-Ling decoction in the treatment of chronic kidney diseases.
    MeSH term(s) Chlorocebus aethiops ; Rats ; Animals ; Hydrogen Peroxide ; Vero Cells ; Mass Spectrometry/methods ; Triterpenes ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/chemistry ; Chromatography, High Pressure Liquid/methods
    Chemical Substances poricoic acid A ; Hydrogen Peroxide (BBX060AN9V) ; Triterpenes ; Drugs, Chinese Herbal
    Language English
    Publishing date 2023-07-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2047990-6
    ISSN 1615-9314 ; 1615-9306
    ISSN (online) 1615-9314
    ISSN 1615-9306
    DOI 10.1002/jssc.202300331
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