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Article: Xiongshao Zhitong Recipe Attenuates Nitroglycerin-Induced Migraine-Like Behaviors

Yang, Song / Chen, Cong / Liu, Xiaoyao / Kang, Qianjun / Ma, Quantao / Li, Pin / Hu, Yujie / Li, Jialin / Gao, Jian / Wang, Ting / Wang, Weiling

Frontiers in pharmacology

2022 Volume 13, Page(s) 920201

Abstract: ... women. Xiongshao Zhitong Recipe (XZR) is a traditional Chinese medicine prescription used for treating ...

Abstract	Migraine is a major cause of disability worldwide, particularly in young adults and middle-aged women. Xiongshao Zhitong Recipe (XZR) is a traditional Chinese medicine prescription used for treating migraine, but its bioactive components and therapeutic mechanisms remain unclear. We aimed to confirm the therapeutic effect of XZR on migraine and to determine the possible mechanism and bioactive components of XZR. Here, a sensitive UHPLC-LTQ-Orbitrap MS assay was carried out to analyze the ingredients of XZR, and a total of 62 components were identified, including coumarins, phenolic acids, phthalides, flavonoids, and terpenoids; among them, 15 components were identified in the serum samples after XZR treatment. We established a rat model of migraine
Language	English
Publishing date	2022-07-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.920201
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A network-based analysis and experimental validation of traditional Chinese medicine Yuanhu Zhitong Formula in treating neuropathic pain.

Liu, Tongtong / Li, Ting / Chen, Xuhui / Zhang, Kaiwen / Li, Meihong / Yao, Wenlong / Zhang, Chuanhan / Wan, Li

Journal of ethnopharmacology

2021 Volume 274, Page(s) 114037

Abstract: Ethnopharmacological relevance: The Yuanhu Zhitong Formula (YZF) consists of traditional Chinese ...

Abstract	Ethnopharmacological relevance: The Yuanhu Zhitong Formula (YZF) consists of traditional Chinese herbs Corydalis Rhizoma (Corydalis yanhusuo (Y.H.Chou & Chun C.Hsu) W.T.Wang ex Z.Y.Su & C.Y.Wu; Chinese name, Yanhusuo) and Angelicae Dahuricae Radix (Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav.; Chinese name, Baizhi), which is usually administrated for painful conditions. It is well acknowledged that YZF has pharmacological effects on pain relief; nevertheless, limited data are available on its mechanism. Aim of the study: This study aimed to explore the potential mechanism underlying YZF on nociception of rats. Also, the comprehensive mechanism of YZF was preliminarily determined based on network pharmacology on neuropathic pain. Materials and methods: A spared nerve injury (SNI) model was established to reveal the effects of YZF administration on nociceptive behavior in rats. Von-Frey tests were used to evaluate the paw withdrawal mechanical thresholds in rats administrated with YZF or vehicle. The "drug-ingredients" and "disease-drug-target" networks were established with a network pharmacology approach. The analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) profiles were performed based on the common targets between the herbs and neuropathic pain. Hub genes, identified with CytoHubba, were validated by Western blotting analysis. Results: SNI rats developed significant nociceptive behavior as soon as 3 days after nerve injury, which was reversed by consecutive treatment with 300 mg/kg YZF for 7 days. Besides, 50 potential bioactive components in YZF with 1074 targets were identified. Then, 217 putative common genes related to YZF and neuropathic pain were identified for further study. After established a protein-protein interaction network, 12 subnetworks with CytoHubba and 10 predictive hub genes were obtained based on the maximal clique centrality model. Western blotting analysis indicated that SNI rats exhibited increased APP (Amyloid-beta precursor protein), SRC (Proto-oncogene tyrosine-protein kinase Src), and phosphorylation of JNK1 (Mitogen-activated protein kinase 8, JNK) and ERK1/2 (Mitogen-activated protein kinase 3/1). Obviously, continuous administration of YZF robustly reversed such changes. Conclusions: This study revealed that YZF modulates the nociceptive behavior in SNI rats. Moreover, the drug may be useful in the treatment of neuropathic pain through multi-components, multi-targets, and multi-pathways. Nevertheless, more attention should be paid to discriminating the potential ingredients in YZF contributing to its analgesic effects in the treatment of neuropathic pain.
MeSH term(s)	Amyloid beta-Protein Precursor/metabolism ; Analgesics/pharmacology ; Analgesics/therapeutic use ; Animals ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Male ; Medicine, Chinese Traditional ; Neuralgia/drug therapy ; Neuralgia/metabolism ; Protein Interaction Maps ; Protein Kinases/metabolism ; Rats, Sprague-Dawley ; Sciatic Nerve/injuries ; Sciatic Neuropathy/drug therapy ; Sciatic Neuropathy/metabolism ; Rats
Chemical Substances	Amyloid beta-Protein Precursor ; Analgesics ; Drugs, Chinese Herbal ; yuanhu zhitong ; Protein Kinases (EC 2.7.-)
Language	English
Publishing date	2021-03-19
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114037
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Article: [Preparation and optimization of zhitong micro-emulsion formula].

Wang, Qiong / Lan, Yi / Chen, Yan-Yan / Dai, Xin-Yuan / An, Jing / Wang, Wen-Ping / Zhao, Bo-Chen / Liu, Na / Zhang, Ye-Wen / Wu, Qing

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2014 Volume 39, Issue 2, Page(s) 222–229

Abstract: To prepare Zhitong micro-emulsion in this study, with the empirical formula of Zhitong preparation ... co-surfactant mass ratio (K(m)). Subsequently, the D-mixture design was used to optimize Zhitong ... were made to determine part of physical parameters of Zhitong micro-emulsion and preliminarily detect ...

Abstract	To prepare Zhitong micro-emulsion in this study, with the empirical formula of Zhitong preparation as the model medicine, the essential oil in the formula as the oil phase, and the water decoction as the water phase. The types of surfactant and co-surfactant were investigated. The changes in micro-emulsion conductivity and construction, the water percentage in the micro-emulsion system, the changing curve of conductivity and the fine pseudo-ternary phase diagram of micro-emulsion were drawn to determine the surfactant-co-surfactant mass ratio (K(m)). Subsequently, the D-mixture design was used to optimize Zhitong Micro-emulsion formula, with particle size and surface tension of micro-emulsion as the indexes. Finally, efforts were made to determine part of physical parameters of Zhitong micro-emulsion and preliminarily detect its stability. The results showed that the micro-emulsion was optimal with the EL-35-tween 20 ratio of 4:1 in surfactant, whereas the absolute ethyl alcohol was recommended as the co-surfactant. The ratio between surfactant and co-surfactant (K(m)) was 1.5. The finalized micro-emulsion formula contains 12% surfactant, 8% co-surfactant, 70% 1 g x mL(-1) water decoction and 8% oil. The results of the preliminary stability experiment showed a better stability of Zhitong micro-emulsion.
MeSH term(s)	Chemistry, Pharmaceutical/methods ; Drugs, Chinese Herbal/chemistry ; Emulsions ; Surface-Active Agents/chemistry ; Temperature
Chemical Substances	Drugs, Chinese Herbal ; Emulsions ; Surface-Active Agents
Language	Chinese
Publishing date	2014-01
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A network-based analysis and experimental validation of traditional Chinese medicine Yuanhu Zhitong Formula in treating neuropathic pain

Liu, Tongtong / Li, Ting / Chen, Xuhui / Zhang, Kaiwen / Li, Meihong / Yao, Wenlong / Zhang, Chuanhan / Wan, Li

Journal of ethnopharmacology. 2021 June 28, v. 274

2021

Abstract: The Yuanhu Zhitong Formula (YZF) consists of traditional Chinese herbs Corydalis Rhizoma (Corydalis ...

Abstract	The Yuanhu Zhitong Formula (YZF) consists of traditional Chinese herbs Corydalis Rhizoma (Corydalis yanhusuo (Y.H.Chou & Chun C.Hsu) W.T.Wang ex Z.Y.Su & C.Y.Wu; Chinese name, Yanhusuo) and Angelicae Dahuricae Radix (Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav.; Chinese name, Baizhi), which is usually administrated for painful conditions. It is well acknowledged that YZF has pharmacological effects on pain relief; nevertheless, limited data are available on its mechanism.This study aimed to explore the potential mechanism underlying YZF on nociception of rats. Also, the comprehensive mechanism of YZF was preliminarily determined based on network pharmacology on neuropathic pain.A spared nerve injury (SNI) model was established to reveal the effects of YZF administration on nociceptive behavior in rats. Von-Frey tests were used to evaluate the paw withdrawal mechanical thresholds in rats administrated with YZF or vehicle. The “drug-ingredients” and “disease-drug-target” networks were established with a network pharmacology approach. The analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) profiles were performed based on the common targets between the herbs and neuropathic pain. Hub genes, identified with CytoHubba, were validated by Western blotting analysis.SNI rats developed significant nociceptive behavior as soon as 3 days after nerve injury, which was reversed by consecutive treatment with 300 mg/kg YZF for 7 days. Besides, 50 potential bioactive components in YZF with 1074 targets were identified. Then, 217 putative common genes related to YZF and neuropathic pain were identified for further study. After established a protein-protein interaction network, 12 subnetworks with CytoHubba and 10 predictive hub genes were obtained based on the maximal clique centrality model. Western blotting analysis indicated that SNI rats exhibited increased APP (Amyloid-beta precursor protein), SRC (Proto-oncogene tyrosine-protein kinase Src), and phosphorylation of JNK1 (Mitogen-activated protein kinase 8, JNK) and ERK1/2 (Mitogen-activated protein kinase 3/1). Obviously, continuous administration of YZF robustly reversed such changes.This study revealed that YZF modulates the nociceptive behavior in SNI rats. Moreover, the drug may be useful in the treatment of neuropathic pain through multi-components, multi-targets, and multi-pathways. Nevertheless, more attention should be paid to discriminating the potential ingredients in YZF contributing to its analgesic effects in the treatment of neuropathic pain.
Keywords	Angelica dahurica ; Corydalis yanhusuo ; Oriental traditional medicine ; analgesia ; analgesics ; gene ontology ; mitogen-activated protein kinase ; models ; nerve tissue ; nociception ; pain ; phosphorylation ; protein-protein interactions ; proto-oncogenes ; rhizomes
Language	English
Dates of publication	2021-0628
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114037
Database	NAL-Catalogue (AGRICOLA)

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Article: [Research of Yuanhu Zhitong prescription based on strategy of integrative pharmacology].

Ma, Yan / Zhang, Ying-chun / Tao, Ye / Chen, Xiao-meng / Wang, Peng / Xu, Hai-yu / Yang, Hong-jun

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2015 Volume 40, Issue 6, Page(s) 1048–1054

Abstract: ... of TCM. For the past years, we applied the strategy of integrative pharmacology to study Yuanhu Zhitong ...

Abstract	Chinese medicinal formulae (CMF) were usually used in the clinics of traditional Chinese medicines (TCM), which were critical for modernization of Chinese medicine to shed light on the interaction between CMF and biological organisms. However, correlation between system and part, macroscopic actions and microcosmic mechanism, ADME process and pharmacologic actions were usually neglected. The put-forward of integrative pharmacology provided a feasible approach to solve the problem of the fragmentation of TCM. For the past years, we applied the strategy of integrative pharmacology to study Yuanhu Zhitong prescription( YZP) systematically, and established two modes, chemical fingerprints-metabolism fingerprints-network targets and intestinal absorption-activity evaluation-data mining, to establish the interaction rule between the chemical composition and biological activity from multiple levels, such as the calculation and in vitro/vivo, which provided proof for the quality control, pharmacodynamic material basis and pharmacological action of YZP. In this paper, we summarized the related progresses of the research of YZP.
MeSH term(s)	Drug Combinations ; Drug Prescriptions ; Drug Therapy ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacology ; Humans ; Molecular Structure
Chemical Substances	Drug Combinations ; Drugs, Chinese Herbal
Language	Chinese
Publishing date	2015-03
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3056: Show issues

Location:
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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: [Study on in vitro release and percutaneous absorption for Zhitong cataplasm].

Song, Li-Hua / Du, Mao-Bo / Liu, Shu-Zhi / Ge, Ke-Ya / Wang, Wen-Ping / Cao, Qi-Chen / Li, Xian-Duan

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2013 Volume 38, Issue 14, Page(s) 2306–2308

Abstract: To evaluate in vitro release and transdermal behaviors of Zhitong cataplasm, modified Franz ... diffusion cell method was applied to investigate in vitro transdermal absorption of Zhitong cataplasm and ... So Zhitong cataplasm had a good release and transdermal properties and transdermal actions were consistent ...

Abstract	To evaluate in vitro release and transdermal behaviors of Zhitong cataplasm, modified Franz diffusion cell method was applied to investigate in vitro transdermal absorption of Zhitong cataplasm and the content of tetrahydropalmatine was determined by HPLC. In 24 hours, accumulative release rate of tetrahydropalmatine was 81. 9%, transmission rate was 2.26 microg x cm(-2) x h(-1). In 48 hours, accumulative transdermal rate and transmission rate of tetrahydropalmatine were 20.31%, 0.22 pg x cm(-2) x h(-1). So Zhitong cataplasm had a good release and transdermal properties and transdermal actions were consistent with zero-order kinetics process.
MeSH term(s)	Administration, Cutaneous ; Animals ; Berberine Alkaloids/administration & dosage ; Berberine Alkaloids/chemistry ; Berberine Alkaloids/pharmacokinetics ; Drugs, Chinese Herbal/administration & dosage ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacokinetics ; Male ; Mice ; Plant Extracts/administration & dosage ; Plant Extracts/chemistry ; Plant Extracts/pharmacokinetics ; Skin/metabolism ; Skin Absorption
Chemical Substances	Berberine Alkaloids ; Drugs, Chinese Herbal ; Plant Extracts ; tetrahydropalmatine (3X69CO5I79)
Language	Chinese
Publishing date	2013-07
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: [Effect of microemulsion technology on release of compounds in Zhitong cataplasm and content variation].

Li, Xue-Min / Wang, Qiong / Lan, Yi / An, Jing / Wang, Wen-Ping / Zhao, Bo-Chen / Wu, Qing

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2013 Volume 38, Issue 16, Page(s) 2614–2617

Abstract: To study the varieties of adhesives in gels on the basis of previous studies, dosage of adhesives required for forming of gels by using the single factor design, as well as the drug loading capacity of gels with the adhesiveness and stickiness as the ... ...

Abstract	To study the varieties of adhesives in gels on the basis of previous studies, dosage of adhesives required for forming of gels by using the single factor design, as well as the drug loading capacity of gels with the adhesiveness and stickiness as the scoring indicators, in order to determine the forming method of analgesic micro-emulsion gel. Subsequently, the improved Franz diffusing cell method was adopted to study the release of water-soluble components and liposoluble components in analgesic gels, with imperatorin and ferulic acid as index components. The results showed that analgesic micro-emulsion gel could promote the release of imperatorin and ferulic acid. Finally, HPLC was used to detect that the loss amount of volatile components in analgesic micro-emulsion gel was 23.13% lower than that in the original analgesic gel. In the experiment, we finally prepared finished products of micro-emulsion gel, discovered that the micro-emulsion technology is helpful to improve the synchronous release of water-soluble components and liposoluble components in prescriptions and can reduce the loss of volatile components.
MeSH term(s)	Adhesiveness ; Analgesics/chemistry ; Analgesics/therapeutic use ; Animals ; Chemistry, Pharmaceutical/methods ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/therapeutic use ; Emulsions ; Hydrophobic and Hydrophilic Interactions ; Mice ; Ointments ; Pain/drug therapy
Chemical Substances	Analgesics ; Drugs, Chinese Herbal ; Emulsions ; Ointments
Language	Chinese
Publishing date	2013-08
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Quantitative and qualitative analysis of common peaks in chemical fingerprint of Yuanhu Zhitong tablet by HPLC-DAD-MS/MS.

Tang, Dao-Quan / Zheng, Xiao-Xiao / Chen, Xu / Yang, Dong-Zhi / Du, Qian

Journal of pharmaceutical analysis

2013 Volume 4, Issue 2, Page(s) 96–106

Abstract: ... in chemical fingerprint was proposed to analyze Yuanhu Zhitong tablet (YZT), using high performance ...

Abstract	A quality control (QC) strategy for quantitative and qualitative analysis of "common peaks" in chemical fingerprint was proposed to analyze Yuanhu Zhitong tablet (YZT), using high performance liquid chromatography with diode array detector and tandem mass spectrometry (HPLC-DAD-MS/MS). The chromatographic separation was achieved on an Agilent Eclipse plus C
Language	English
Publishing date	2013-12-28
Publishing country	China
Document type	Journal Article
ZDB-ID	2630174-X
ISSN	2095-1779
ISSN	2095-1779
DOI	10.1016/j.jpha.2013.12.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Advances in Plasma Diagnostics and Applications

Chen, Zhitong / Attri, Pankaj / Wang, Qiu

2022

Keywords	Technology: general issues ; History of engineering & technology ; gliding arc plasma ; discharge characteristics ; swirl flame ; static instability control ; non-thermal plasma ; plasma agriculture ; seed germination ; growth enhancement ; germination ; Mg deposition ; plasma treatment ; surface modification ; SparkJet actuator ; shock wave control ; supersonic flow ; schlieren images ; dynamic pressure measurement ; plasma chemical vapor deposition ; carbon nanoparticle ; coagulation ; optical emission spectroscopy ; plasma spray welding ; WC-Co coating ; Fe-based amorphous alloy ; microstructure and properties ; cold atmospheric plasma ; tiny plasma jet ; biomedical applications ; oblique detonation ; asymmetric ; nozzle ; reflection ; n/a
Language	0\|e
Size	1 electronic resource (124 pages)
Publisher	MDPI Books
Publishing place	Basel
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021618806
ISBN	9783036543208 ; 3036543201
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Study on the absorbed fingerprint-efficacy of yuanhu zhitong tablet based on chemical analysis, vasorelaxation evaluation and data mining.

Xu, Haiyu / Li, Ke / Chen, Yanjun / Zhang, Yingchun / Tang, Shihuan / Wang, Shanshan / Shen, Dan / Wang, Xuguang / Lei, Yun / Li, Defeng / Zhang, Yi / Jin, Lan / Yang, Hongjun / Huang, Luqi

PloS one

2013 Volume 8, Issue 12, Page(s) e81135

Abstract: Yuanhu Zhitong Tablet (YZT) is an example of a typical and relatively simple clinical herb formula ...

Abstract	Yuanhu Zhitong Tablet (YZT) is an example of a typical and relatively simple clinical herb formula that is widely used in clinics. It is generally believed that YZT play a therapeutical effect in vivo by the synergism of multiple constituents. Thus, it is necessary to build the relationship between the absorbed fingerprints and bioactivity so as to ensure the quality, safety and efficacy. In this study, a new combinative method, an intestinal absorption test coupled with a vasorelaxation bioactivity experiment in vitro, was a simple, sensitive, and feasible technique to study on the absorbed fingerprint-efficacy of YZT based on chemical analysis, vasorelaxation evaluation and data mining. As part of this method, an everted intestinal sac method was performed to determine the intestinal absorption of YZT solutions. YZT were dissolved in solution (n = 12), and the portion of the solution that was absorbed into intestinal sacs was analyzed using rapid-resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q-TOF/MS). Semi-quantitative analysis indicated the presence of 34 compounds. The effect of the intestinally absorbed solution on vasorelaxation of rat aortic rings with endothelium attached was then evaluated in vitro. The results showed that samples grouped by HCA from chemical profiles have similar bioactivity while samples in different groups displayed very different. Moreover, it established a relationship between the absorbed fingerprints and their bioactivity to identify important components by grey relational analysis, which could predict bioactive values based on chemical profiles and provide an evidence for the quantification of multi-constituents.
MeSH term(s)	Animals ; Cluster Analysis ; Data Mining ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/metabolism ; Drugs, Chinese Herbal/pharmacology ; In Vitro Techniques ; Intestinal Absorption ; Male ; Mass Spectrometry ; Rats ; Rats, Sprague-Dawley ; Tablets ; Vasodilation/drug effects
Chemical Substances	Drugs, Chinese Herbal ; Tablets ; yuanhu zhitong
Language	English
Publishing date	2013-12-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0081135
Database	MEDical Literature Analysis and Retrieval System OnLINE

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