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Article ; Online: Primary adenocarcinoma of colon: A clinicopathological study with the prevalence and correlation of CDX2 biomarker expression - A tertiary care center experience.

Issac, Rijo / Masih, Dipti / Ranjan, Mark / Pulimood, Anna B

2023

Abstract: Background: Colorectal cancer is one of the alarming health problems worldwide. Prognostic biomarkers are the key for risk stratification in patients with colon cancer and the decision to recommend adjuvant chemotherapy. It has been difficult to ... ...

Abstract	Background: Colorectal cancer is one of the alarming health problems worldwide. Prognostic biomarkers are the key for risk stratification in patients with colon cancer and the decision to recommend adjuvant chemotherapy. It has been difficult to identify a single prognostic biomarker for colon cancer. Currently, tumor stage, tumor grade, and microsatellite instability remain the most important prognostic variables that aid in the treatment of patients with colon cancer. Some studies highlighted that CDX2 immunohistochemistry negativity is an independent prognostic factor and indicates a worse survival rate. Our aim was to study the prevalence of CDX2 biomarker expression in patients diagnosed with primary adenocarcinoma and to correlate this with the clinical profile and pathological features. Methods: Endoscopic mucosal biopsies and resection specimens of 148 patients diagnosed with colonic adenocarcinoma were analyzed. CDX2 immunohistochemistry was performed, and the result was correlated with clinicopathological features. The results were presented as mean, frequencies, and percentages. Pearson's Chi-square test was used to assess the associations between clinicopathological parameters and CDX2 immunohistochemistry negativity. Results: The prevalence of CDX2 expression by immunohistochemistry in colon cancer was found to be 92%. CDX2 biomarker negativity was found to be higher in left-sided colon cancers, poorly differentiated adenocarcinoma, mucinous carcinoma, and higher TNM stages. Conclusion: CDX2-negative tumors are often associated with several adverse prognostic variables (e.g., advanced stage, poor differentiation, and metastasis). Thus, sub-classification of colon cancer based on the CDX2 biomarker aids to separate them into prognostically relevant categories.
Language	English
Publishing date	2023-04-17
Publishing country	India
Document type	Journal Article
ZDB-ID	410194-7
ISSN	1998-4774 ; 0019-509X
ISSN (online)	1998-4774
ISSN	0019-509X
DOI	10.4103/ijc.ijc_760_21
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Article ; Online: Granulomatous inflammation is less common in delayed-onset Crohn's disease.

Barnaba Durairaj, Madeline Vithya / Jaleel, Rajeeb / Pulimood, Anna B

Tropical doctor

2022 Volume 53, Issue 1, Page(s) 113–116

Abstract: Crohn's disease (CD) usually affects younger individuals but delayed-onset disease is not uncommon. We studied the epidemiology, demographic profile, and clinical characteristics of patients with delayed-onset CD (disease onset >50 years) and compared ... ...

Abstract	Crohn's disease (CD) usually affects younger individuals but delayed-onset disease is not uncommon. We studied the epidemiology, demographic profile, and clinical characteristics of patients with delayed-onset CD (disease onset >50 years) and compared them with CD in younger individuals (disease onset 20-40 years) in a tertiary care center in India. The presenting symptoms, site of involvement, and treatment profile were similar, except for weight loss, which was more often noted in young-onset CD. However, granulomatous inflammation on mucosal biopsy was twice as common in young-onset compared to delayed-onset CD. As it is thus seen less often in delayed-onset CD, this may lead to a potential delay in diagnosis and treatment.
MeSH term(s)	Humans ; Crohn Disease/diagnosis ; Crohn Disease/epidemiology ; Biopsy ; Inflammation/diagnosis ; Inflammation/epidemiology ; India/epidemiology
Language	English
Publishing date	2022-07-29
Publishing country	England
Document type	Journal Article
ZDB-ID	193169-6
ISSN	1758-1133 ; 0049-4755
ISSN (online)	1758-1133
ISSN	0049-4755
DOI	10.1177/00494755221104645
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Article ; Online: Concerns with regard to an article.

George, Biju / Pulimood, Anna

Indian journal of medical ethics

2019 Volume 4, Issue 3, Page(s) 251

Abstract: We have read with interest and concern the article titled "Consultations on human infection studies in India: Do people's voices really count" by Sandhya Srinivasan and Veena Johari. The article expresses the opinions of 2 persons who have not visited ... ...

Abstract	We have read with interest and concern the article titled "Consultations on human infection studies in India: Do people's voices really count" by Sandhya Srinivasan and Veena Johari. The article expresses the opinions of 2 persons who have not visited CMC, nor have spoken to anyone concerned, to ascertain the authenticity of the information published or with regard to the purported research in CMC.
MeSH term(s)	Humans ; India ; Referral and Consultation ; Research
Language	English
Publishing date	2019-04-05
Publishing country	India
Document type	Letter ; Comment
ISSN	0975-5691
ISSN (online)	0975-5691
DOI	10.20529/IJME.2019.014
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Article ; Online: Amino acid sequence divergence of Tat protein (exon1)of subtype B and C HIV-1 strains: Does it have implications for vaccine development?

Kandathil, Abraham Joseph / Kannangai, Rajesh / Abraham, Oriapadickal Cherian / Pulimood, Susanne Alexander / Sridharan, Gopalan

Bioinformation

2009 Volume 4, Issue 6, Page(s) 237–241

Abstract: ... of subtype B and C strains of HIV-1 and tried to observe a molecular basis for protein function. HIV-1 ... sequences of subtype B (n=30) and C (n=60) strains were downloaded from HIV-1 Los Alamos data base ... sequence, the consensus B and C sequence was obtained from the 'sequence search interface' in the Los Alamos HIV-1 ...

Abstract	Functional genes of HIV-1 like the tat express proteins essential for viral survival and propagation. There are variations reported in levels of Tat transactivation among the different subtypes of HIV-1. This study looked at the amino acid differences in the different regions of Tat protein (exon 1) of subtype B and C strains of HIV-1 and tried to observe a molecular basis for protein function. HIV-1 sequences of subtype B (n=30) and C (n=60) strains were downloaded from HIV-1 Los Alamos data base. Among the 60 subtype C strain sequences, 30 each were from India and Africa. A HIV-1 Tat protein (exon 1) sequence, the consensus B and C sequence was obtained from the 'sequence search interface' in the Los Alamos HIV-1 sequence data. The sequences were visualized using Weblogo and the RNA binding regions of the three consensus sequences were also determined using BindN software program. Compared to subtype B, there was a high level of divergence in the auxiliary domain of tat exon 1 (amino acid positions 58- 69). The net charge of the subtype C (Indian) Tat protein (exon 1) auxiliary domain was -1.9 at pH 7 and it had an isoelectric point of 4.1. The net charge of the subtype C (African) auxiliary domain was -2.9 at pH 7 and it had an isoelectric point of 3.7 while the net charge of same region in subtype B was -0.9 at pH 7 with an isoelectric point of 4.9. The ratio of the hydrophilic residues to the total number of residues was 60% in the in both the Indian and African subtype C in the auxiliary domain while this was 50% in subtype B. The consensus subtype B sequence was found to have 36 RNA binding sites while subtype C (India) had 33 and subtype C (Africa) had 32 RNA binding sites. The HIV-1 Tat-TAR interaction is a potential target for inhibitors and being considered for its potential use in HIV-1 vaccines. Development of such inhibitor/vaccines would have to take into consideration the variation in amino acid sequence analyzed in this study as this could determine epitope presentation on MHC class I antigen for afferent immune response.
Language	English
Publishing date	2009-12-12
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2203786-X
ISSN	0973-2063 ; 0973-2063
ISSN (online)	0973-2063
ISSN	0973-2063
DOI	10.6026/97320630004237
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Article ; Online: A comparison of interpretation by three different HIV type 1 genotypic drug resistance algorithms using sequences from non-clade B HIV type 1 strains.

Kandathil, Abraham Joseph / Kannangai, Rajesh / Abraham, Oriapadickal Cherian / Pulimood, Susanne Alexander / Jensen, Mark A / Sridharan, Gopalan

AIDS research and human retroviruses

2009 Volume 25, Issue 3, Page(s) 315–318

Abstract: ... a paucity of information on the performance of genotypic algorithms on non-clade B HIV-1 strains, especially ... clade C. In this study the results obtained on submission of HIV-1 RT and PR sequences of non-clade B ...

Abstract	The advent of affordable ART has benefited HIV-infected individuals. Prospective studies have shown that the availability of drug resistance reports for infected individuals has allowed more effective regimens to be prescribed as compared to a control group whose physicians had no access to drug resistance reports. There is a paucity of information on the performance of genotypic algorithms on non-clade B HIV-1 strains, especially clade C. In this study the results obtained on submission of HIV-1 RT and PR sequences of non-clade B strains to the Stanford University HIV drug resistance database (SHDB) were compared to the results obtained from Geno2Pheno (G2P) and DR_Seqan (DS). For the study, we took samples from 93 treatment-naive individuals and 21 samples from 19 infected individuals showing detectable viral load while on ART. There were discrepancies in the clade identification results obtained from the SHDB and G2P databases. This feature was not available in DS. The mean observed concordance between SHDB and G2P was 85.6% while between SHDB and DC it was 37%. When the level of concordance was determined based on exposure to ART, the G2P was found to have a better level of concordance (76.8%) to SHDB as compared to SHDB versus DS (36%). We do not have phenotypic data for the strains included in this study and hence we are not in a position to assign a particular algorithm as being superior. These results also show a possible need for a subtype-specific algorithm for interpretation of HIV-1 genotypic drug resistance.
MeSH term(s)	Algorithms ; Cluster Analysis ; Computational Biology/methods ; Drug Resistance, Viral ; Genotype ; HIV Infections/virology ; HIV Protease/genetics ; HIV Reverse Transcriptase/genetics ; HIV-1/drug effects ; HIV-1/genetics ; Humans ; Microbial Sensitivity Tests/methods ; Molecular Sequence Data ; Sequence Analysis, DNA
Chemical Substances	reverse transcriptase, Human immunodeficiency virus 1 (EC 2.7.7.-) ; HIV Reverse Transcriptase (EC 2.7.7.49) ; HIV Protease (EC 3.4.23.-) ; p16 protease, Human immunodeficiency virus 1 (EC 3.4.23.-)
Language	English
Publishing date	2009-03
Publishing country	United States
Document type	Comparative Study ; Evaluation Studies ; Journal Article
ZDB-ID	639130-8
ISSN	1931-8405 ; 0889-2229
ISSN (online)	1931-8405
ISSN	0889-2229
DOI	10.1089/aid.2008.0177
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Article ; Online: Hyperacute Graft-Versus-Host Disease of the Skin following Allogeneic Stem Cell Transplantation: A Paediatric Case Series.

Mahabal, Gauri D / George, Leni / Peter, Dincy / Thomas, Meera / George, Biju / Mathews, Vikram / Abraham, Aby / Srivastava, Alok / Pulimood, Susanne

Indian journal of dermatology

2023 Volume 68, Issue 1, Page(s) 73–77

Abstract: Hyperacute GVHD (HaGVHD) is a rare complication of hematopoietic stem cell transplantation (HSCT) occurring before engraftment, a syndrome commonly involving skin and/or gut and/or liver, with increased morbidity and mortality. Myeloablative conditioning ...

Abstract	Hyperacute GVHD (HaGVHD) is a rare complication of hematopoietic stem cell transplantation (HSCT) occurring before engraftment, a syndrome commonly involving skin and/or gut and/or liver, with increased morbidity and mortality. Myeloablative conditioning (MAC) regimes and mismatched donor transplants have an increased risk for HaGVHD. There is a higher chance of steroid-refractoriness and chronic GVHD in those who develop HaGVHD. There is limited literature about HaGVHD, especially in the paediatric age group. This retrospective single-centre case series included five paediatric patients who underwent HSCT between 1
Language	English
Publishing date	2023-05-06
Publishing country	India
Document type	Journal Article
ZDB-ID	416069-1
ISSN	1998-3611 ; 0019-5154
ISSN (online)	1998-3611
ISSN	0019-5154
DOI	10.4103/ijd.ijd_503_22
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Article ; Online: An Unusual Presentation of Hemorrhagic Disease in an Infant: A Probable Case of Abetalipoproteinemia.

Sivamurukan, Palanisamy / Boddu, Deepthi / Pulimood, Anna / Agarwal, Indira

Journal of pediatric hematology/oncology

2020 Volume 43, Issue 3, Page(s) e429–e430

Abstract: ... apolipoprotein B-containing lipoproteins, fat malabsorption, hypocholesterolemia, retinitis pigmentosa ...

Abstract	We report a probable case of abetalipoproteinemia in an infant who presented with unusual symptoms of late-onset vitamin K deficiency. Abetalipoproteinemia is a rare autosomal recessive disease caused by mutation of the microsomal triglyceride transfer protein gene, resulting in the absence of microsomal triglyceride transfer protein function in the small bowel. It is characterized by the absence of plasma apolipoprotein B-containing lipoproteins, fat malabsorption, hypocholesterolemia, retinitis pigmentosa, progressive neuropathy, myopathy, and acanthocytosis. A biopsy of the small intestine characteristically shows marked lipid accumulation in the villi of enterocytes. Large supplements of fat-soluble vitamins A, D, E, and K have been shown to limit neurologic and ocular manifestations. Dietary fat intake is limited to medium-chain triglycerides.
MeSH term(s)	Abetalipoproteinemia/blood ; Abetalipoproteinemia/complications ; Abetalipoproteinemia/diagnosis ; Abetalipoproteinemia/pathology ; Duodenum/pathology ; Enterocytes/pathology ; Female ; Humans ; Infant ; Infant, Newborn ; Vitamin K Deficiency/blood ; Vitamin K Deficiency/complications ; Vitamin K Deficiency/diagnosis ; Vitamin K Deficiency/pathology
Language	English
Publishing date	2020-05-13
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	1231152-2
ISSN	1536-3678 ; 1077-4114 ; 0192-8562
ISSN (online)	1536-3678
ISSN	1077-4114 ; 0192-8562
DOI	10.1097/MPH.0000000000001831
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Article: Lack of Association of

Joseph, Neenu Anna / Mathews, Suma Susan / Anandan, Shalini / Veeraraghavan, Balaji / Bakthavatchalam, Yamuna Devi / Pulimood, Anna B

Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India

2021 Volume 74, Issue Suppl 2, Page(s) 2636–2642

Abstract: To study the association ... ...

Abstract	To study the association of
Language	English
Publishing date	2021-01-08
Publishing country	India
Document type	Journal Article
ZDB-ID	1471137-0
ISSN	0973-7707 ; 2231-3796 ; 0019-5421
ISSN (online)	0973-7707
ISSN	2231-3796 ; 0019-5421
DOI	10.1007/s12070-020-02361-3
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Article ; Online: Comparison of Magnetic Resonance Enterography Global Score (MEGS) with indices of Crohn's disease activity in South Asian population.

Jose, Susanna K / Simon, Betty / Simon, Ebby George / Eapen, Anu / John, Reetu A / Putta, Tharani / Dutta, Amit Kumar / Pulimood, Anna B

Abdominal radiology (New York)

2021 Volume 47, Issue 2, Page(s) 547–553

Abstract: Purpose: Assessment of disease activity in Crohn's helps predict important clinical outcomes. Among the various modalities available to assess disease activity, magnetic resonance enterography (MRE) is considered a safe and reliable imaging option. ... ...

Abstract	Purpose: Assessment of disease activity in Crohn's helps predict important clinical outcomes. Among the various modalities available to assess disease activity, magnetic resonance enterography (MRE) is considered a safe and reliable imaging option. Various MRE-based scoring systems have been developed to measure disease activity, one of which being the MRE global score (MEGS). We aimed to correlate MEGS with some of the important indices of Crohn's disease activity. Methodology: Crohn's disease patients referred for MRE were included in the study. Along with demographic profile and relevant investigations, MRE parameters related to MEGS were also assessed. Result: A total of 47 patients were recruited for the study. Their median age was 34 years (range 18-68 years), and male:female ratio was 16:31. There was modest positive correlation between MEGS and faecal calprotectin (r = 0.3, p = 0.04), CRP level (r = 0.34, p = 0.02) and Harvey Bradshaw index (r = 0.3, p = 0.043), respectively. However, there was strong correlation between segmental MEGS and Simple Endoscopic Score in those with terminal ileal disease (r = 0.81, p < 0.001). Mural thickness was the only MRE parameter that correlated with active disease (OR - 1.35, 95% CI 1.01, 1.81, p = 0.041) on multivariate analysis. There was moderate inter-observer agreement (Lin's r = 0.78, p < 0.001). Conclusion: MEGS showed modest correlation with indices of Crohn's disease activity which corroborates the complementary role of MRE in management of such patients.
MeSH term(s)	Adolescent ; Adult ; Aged ; Crohn Disease/diagnostic imaging ; Crohn Disease/pathology ; Female ; Humans ; Ileum ; Leukocyte L1 Antigen Complex ; Magnetic Resonance Imaging/methods ; Magnetic Resonance Spectroscopy ; Male ; Middle Aged ; Young Adult
Chemical Substances	Leukocyte L1 Antigen Complex
Language	English
Publishing date	2021-12-27
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2839786-1
ISSN	2366-0058 ; 2366-004X
ISSN (online)	2366-0058
ISSN	2366-004X
DOI	10.1007/s00261-021-03381-5
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Article: The prevalence of markers for hepatitis B and human immunodeficiency viruses, malarial parasites and microfilaria in blood donors in a large hospital in south India.

Singhvi, A / Pulimood, R B / John, T J / Babu, P G / Samuel, B U / Padankatti, T / Carman, R H

The Journal of tropical medicine and hygiene

1990 Volume 93, Issue 3, Page(s) 178–182

Abstract: ... 46%), Rh positive (93.93%). Hepatitis B surface antigen was found in 1.37% and 2.96% of voluntary and ...

Abstract	Blood donors (35,395) were screened at the CMC Hospital, Vellore, in South India during the 2-year period from July 1986 to June 1988. They were either relatives of patients (referred to as replacement donors) or volunteers. Paid or professional donors were not accepted. Of the replacements 21.5% and of the voluntary donors 9.7% were rejected. Mean donor age was 32.2 years and the commonest blood group was O (39.46%), Rh positive (93.93%). Hepatitis B surface antigen was found in 1.37% and 2.96% of voluntary and replacement donors respectively. Anti-HIV antibodies were found in 1.9/1000 donors. This shows an increasing prevalence of HIV infection in the Indian donor population not reported earlier. The prevalences of malarial parasites and microfilaria in replacement donors were 0.02 and 1.62% respectively. In voluntary donors, malarial parasites were not found and microfilaria were found in only 0.15%. All rejection criteria except low haemoglobin were significantly more frequent among replacement donors than among voluntary donors. In the light of these results, screening of all donor blood for HBsAg and Anti-HIV by the most sensitive techniques and for malarial and filarial parasites in endemic areas is essential.
MeSH term(s)	Adolescent ; Adult ; Blood Donors ; Blood Grouping and Crossmatching ; Blotting, Western ; Enzyme-Linked Immunosorbent Assay ; Female ; Filariasis/blood ; Filariasis/epidemiology ; HIV Antibodies/analysis ; HIV Infections/blood ; HIV Infections/epidemiology ; Hepatitis B/blood ; Hepatitis B/epidemiology ; Hepatitis B Surface Antigens/analysis ; Humans ; India/epidemiology ; Malaria/blood ; Malaria/epidemiology ; Male ; Middle Aged ; Prevalence
Chemical Substances	HIV Antibodies ; Hepatitis B Surface Antigens
Language	English
Publishing date	1990-06
Publishing country	England
Document type	Journal Article
ZDB-ID	218345-6
ISSN	0022-5304
ISSN	0022-5304
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